Pisum_sativum_v1_Contig2064

Contig Overview
NamePisum_sativum_v1_Contig2064
Unique NamePisum_sativum_v1_Contig2064
Typecontig
OrganismPisum sativum (pea)
Sequence length521
Unigenes
This contig is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1contig
Relationships

The following EST feature(s) are a part of this contig:

Feature NameUnique NameSpeciesType
GH720694GH720694Pisum sativumEST
GH720000GH720000Pisum sativumEST


Analyses
This contig is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
InterProScan analysis for Pisum sativum unigene v12010-12-29
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
GH720694ESTGH720694:1..521 -
GH720000ESTGH720000:23..500 +
Homology
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: CHIT2_TULBA (Chitinase 2 OS=Tulipa bakeri PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.647e-14
Identity = 51/146 (34.93%), Postives = 75/146 (51.37%), Query Frame = -1 
Query:   39 IQMDFILGFASEHYIDGKG----DGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGV---GTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAP 455
            +   FIL FA + Y  G      +G FNP W+ +  S  +V   K+ +  V+V +S+GG    G    FNP     W+DNA  S+  II+ Y         +DGIDI Y+      + F+ CIGQ++ +LK +  ++F   VSIAP
Sbjct:   25 VDFHFILAFAID-YTSGSSPTPTNGNFNPFWDTNNLSPSQVAAIKRTYNNVKVSVSLGGNSVGGERVFFNPSSVSSWVDNAVSSLTKIIKQYH--------LDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNGVVSF---VSIAP 158
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: CHIT1_TULBA (Chitinase 1 OS=Tulipa bakeri PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.795e-13
Identity = 49/146 (33.56%), Postives = 75/146 (51.37%), Query Frame = -1 
Query:   39 IQMDFILGFASEHYIDGKG----DGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGV---GTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAP 455
            +   FIL FA + Y  G      +G F P W+ +  S  +V   K+ H  V+V +S+GG    G    F+P     W++NA  S+  II+ Y         +DGIDI Y+      + F+ CIGQ++ +LK ++ ++F   VSIAP
Sbjct:   51 VDFHFILAFAID-YTSGSSPTPTNGNFKPFWDTNNLSPSQVAAVKRTHSNVKVSLSLGGDSVGGKNVFFSPSSVSSWVENAVSSLTRIIKQYH--------LDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSF---VSIAP 184
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Match: RUAP_SOYBN (RuBisCO-associated protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.763e-10
Identity = 38/128 (29.69%), Postives = 68/128 (53.12%), Query Frame = -1 
Query:   75 LGFASEHYIDGKGDGRFNPTWEVDPSSIERVKKFKQGHP----EVRVFISIGGVGTEFPF---NPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDK 437
            L  A ++  +   +G+F P W+ +  + E +KKFK+ +      V+V +SIG    +FPF   +   +  W+  A  S++ II+ Y+        +DGID+ Y+ I ++  DF   +G ++R LK +K
Sbjct:   33 LSLARDYDGNNSTNGKFIPYWDTEKVTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYN--------LDGIDVSYEDIAANEADFVNSVGGLVRNLKQNK 152
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41675_VICNA (Narbonin OS=Vicia narbonensis PE=2 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.724e-28
Identity = 72/157 (45.86%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   I++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDFPTEIINT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q08884_VICNA (Narbonin OS=Vicia narbonensis PE=1 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.724e-28
Identity = 72/157 (45.86%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   I++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDFPTEIINT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q7DLM0_VICNA (Narbonin OS=Vicia narbonensis PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 8.296e-28
Identity = 71/157 (45.22%), Postives = 100/157 (63.69%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   +++  E ++  +ILGFA E Y + GKG G F  +W+V+    E+VK  K+ HPEV+V ISIGG G   PF+P E  VW+ NA +S++ II+ Y DD    N+IDGIDIHY+ I S  + F+  +GQ+I +LK D  L    VVSIAP++
Sbjct:   19 LHDSPTEIVNT--ETLEFHYILGFAIESYYESGKGTGTFEESWDVELFGPEKVKNLKRRHPEVKVVISIGGRGVNTPFDPAEENVWVSNAKESLKLIIQKYSDDSG--NLIDGIDIHYEHIRSD-EPFATLMGQLITELKKDDDLNIN-VVSIAPSE 169
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q08883_9FABA (Narbonin OS=Vicia pannonica PE=3 SV=2)

HSP 1 Score: 125.561 bits (314), Expect = 1.848e-27
Identity = 72/157 (45.86%), Postives = 98/157 (62.42%), Query Frame = -1 
Query:   33 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 500
            +H  P   ID+  E ++  FILGFA+E Y + GK  G F  +W+V+    E VK  K  HPEV+V ISI G   + PF+P E  +W+  A KS++ II+ Y ++    N+IDGIDI+Y+ INS  + F  CIGQVIR+LK D  L    VVSIAP++
Sbjct:   19 LHDFPHEIIDT--ENLEFHFILGFATESYYESGKSTGNFEESWDVELFGPENVKNLKTKHPEVKVVISIRGHDDKTPFDPDEENIWVWKAVKSLKQIIKKYRNESG--NMIDGIDINYEHINSDDELFVNCIGQVIRELKKDDDLNID-VVSIAPSE 170
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41680_9FABA (Narbonin OS=Vicia pannonica PE=2 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 2.414e-27
Identity = 69/162 (42.59%), Postives = 104/162 (64.20%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   ID+  E ++  FILGFA+E+Y    KG G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++IDGIDI+Y+ I SS + F+  +GQ+I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPSEIIDT--ENLEFHFILGFATENYNQARKGTGTFEESWDSESFGLENVKKLKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNESG--SMIDGIDINYEHI-SSDEPFARLVGQLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q39888_SOYBN (Putative narbonin OS=Glycine max PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 9.173e-27
Identity = 68/162 (41.98%), Postives = 103/162 (63.58%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYID-GKGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   ID+  E ++  FILGFA+E Y + GK  G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++IDGIDI+Y+ I SS + F+  +G +I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPHEIIDT--ENLEFHFILGFATESYYESGKSTGNFEESWDSESFGLENVKKLKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNESG--SMIDGIDINYEHI-SSDEPFARLVGLLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41679_9FABA (Narbonin (Fragment) OS=Vicia pannonica PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 7.765e-26
Identity = 67/162 (41.36%), Postives = 103/162 (63.58%), Query Frame = -1 
Query:   18 IHHIPKHRIDSDVERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQVVESY 500
            +H  P   I +  E ++  FILGFA+E+Y    KG G F  +W+ +   +E VKK K+ +PEV+V ISIGG G + PF+P E  VW++NA +S++ I + Y ++    ++I+GIDI+Y+ I SS + F+  +GQ+I +LK D  L    VVSIAP++   S+
Sbjct:   19 LHDFPSEIIGT--ENLEFHFILGFATENYNQARKGTGTFEESWDSESFGLENVKKSKERYPEVKVVISIGGRGVQTPFHPAEENVWVENAQESLKQIFQKYSNENG--SMIEGIDINYEHI-SSDEPFARLVGQLITELKKDDDLNIN-VVSIAPSETNSSH 174
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41704_VICSA (Putative narbonin-like 2S protein OS=Vicia sativa PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.951e-25
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E I+  FILGFA+E Y +G KG G F  +W  +    ++VK  K  HPEV+V ISIGG G E PF+P E  +W+ NA KS++ II+ Y ++    N+IDGIDI+Y+ I S  + F   IGQ+I +LK ++ L    VVSIAP++
Sbjct:   29 ETIEFHFILGFATEKYNEGRKGTGNFEESWMDEFFGPDKVKNLKTKHPEVKVVISIGGRGVETPFDPAEQNIWVSNAVKSLKLIIQKYKNESG--NLIDGIDINYEHIKSD-EAFPRLIGQLITELKKERDLNIH-VVSIAPSE 168
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41660_VICFA (Putative narbonin-like 2S protein OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.951e-25
Identity = 67/144 (46.53%), Postives = 93/144 (64.58%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E I+  FILGFA+E Y +G KG G F  +W  +    ++VK  K  HPEV+V ISIGG G E PF+P E  +W+ NA KS++ II+ Y ++    N+IDGIDI+Y+ I S  + F   IGQ+I +LK ++ L    VVSIAP++
Sbjct:   29 ETIEFHFILGFATEKYNEGRKGTGNFEESWMDEFFGPDKVKNLKTKHPEVKVVISIGGRGVETPFDPAEQNIWVSNAVKSLKLIIQKYKNESG--NLIDGIDINYEHIKSD-EAFPRLIGQLITELKKERDLNIH-VVSIAPSE 168
[Close]
BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Match: Q41663_VICFA (Nodulin homologous to narbonin OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.574e-25
Identity = 66/144 (45.83%), Postives = 95/144 (65.97%), Query Frame = -1 
Query:   33 ERIQMDFILGFASEHYIDG-KGDGRFNPTWEVDPSSIERVKKFKQGHPEVRVFISIGGVGTEFPFNPVENLVWIDNAAKSIQHIIELYDDDQSDQNIIDGIDIHYDVINSSHDDFSYCIGQVIRQLKLDKHLAFKMVVSIAPTQ 461
            E  +  FILGFA+E+Y +  KG G F  +W+ +    ++VK  K+ HPEV+V ISIGG G E PF+P E +VW+ NA +S++ II+ Y ++    N+IDGIDI+Y  I S  + F   IGQ+IR+LK ++ L    VVSIAP++
Sbjct:   29 ETTEFHFILGFATENYSESRKGSGNFVESWKDEFFGPDKVKILKRKHPEVKVVISIGGHGLETPFDPAEQIVWVSNAVRSLKLIIQKYKNESG--NLIDGIDINYGNIKSD-EAFPRLIGQLIRELKKERDLNIH-VVSIAPSE 168
The following BLAST results are available for this feature:
BLAST of Pisum_sativum_v1_Contig2064 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
CHIT2_TULBA7.647e-1434.93Chitinase 2 OS=Tulipa bakeri PE=1 SV=1[more]
CHIT1_TULBA3.795e-1333.56Chitinase 1 OS=Tulipa bakeri PE=1 SV=1[more]
RUAP_SOYBN1.763e-1029.69RuBisCO-associated protein OS=Glycine max PE=2 SV=... [more]
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BLAST of Pisum_sativum_v1_Contig2064 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q41675_VICNA3.724e-2845.86Narbonin OS=Vicia narbonensis PE=2 SV=2[more]
Q08884_VICNA3.724e-2845.86Narbonin OS=Vicia narbonensis PE=1 SV=2[more]
Q7DLM0_VICNA8.296e-2845.22Narbonin OS=Vicia narbonensis PE=3 SV=1[more]
Q08883_9FABA1.848e-2745.86Narbonin OS=Vicia pannonica PE=3 SV=2[more]
Q41680_9FABA2.414e-2742.59Narbonin OS=Vicia pannonica PE=2 SV=2[more]
Q39888_SOYBN9.173e-2741.98Putative narbonin OS=Glycine max PE=3 SV=1[more]
Q41679_9FABA7.765e-2641.36Narbonin (Fragment) OS=Vicia pannonica PE=2 SV=1[more]
Q41704_VICSA2.951e-2546.53Putative narbonin-like 2S protein OS=Vicia sativa ... [more]
Q41660_VICFA2.951e-2546.53Putative narbonin-like 2S protein OS=Vicia faba PE... [more]
Q41663_VICFA6.574e-2545.83Nodulin homologous to narbonin OS=Vicia faba PE=2 ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 7..22
score
IPR0006772S globulinPRINTSPR005512SGLOBULINcoord: 17..36
score: 2.5e-15coord: 59..77
score: 2.5e-15coord: 82..96
score: 2.5
IPR001223Glycoside hydrolase, family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 40..160
score: 7.3
IPR013781Glycoside hydrolase, subgroup, catalytic coreGENE3D3.20.20.80no descriptioncoord: 27..162
score: 3.2
IPR017853Glycoside hydrolase, catalytic coreSUPERFAMILY51445(Trans)glycosidasescoord: 27..161
score: 2.1

Sequences
The following sequences are available for this feature:

contig sequence

>Pisum_sativum_v1_Contig2064 ID=Pisum_sativum_v1_Contig2064; Name=Pisum_sativum_v1_Contig2064; organism=Pisum sativum; type=contig; length=521bp
GCGTGGTCGCGGCCGAGGTACGATTCCACCACTTGTGTTGGAGCAATAGA
TACTACCATTTTGAATGCTAAATGTTTATCAAGTTTGAGTTGTCTTATAA
CTTGTCCAATACAGTAGGAAAAATCATCATGACTTGAATTGATCACATCA
TAATGAATATCAATACCATCAATTATATTTTGATCGCTTTGATCATCGTC
ATAGAGTTCGATGATGTGTTGGATCGATTTTGCAGCATTATCGATCCACA
CCAAATTTTCAACTGGATTGAATGGAAATTCAGTGCCAACACCTCCAATG
CTGATAAACACCCTCACTTCTGGATGACCTTGTTTAAACTTTTTCACCCG
TTCAATACTGGAAGGATCAACCTCCCAAGTGGGGTTGAAACGTCCGTCGC
CTTTTCCATCAATGTAATGCTCGCTGGCAAATCCGAGAATAAAGTCCATT
TGTATTCTCTCGACATCGGAATCGATTCTGTGTTTTGGTATATGATGTAT
GTACCTGCCCGNGGCGGCCGC
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Annotated Terms
The following terms have been associated with this contig:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0043169cation binding
Vocabulary: INTERPRO
TermDefinition
IPR000677Chitinase-like
IPR001223Glyco_hydro18_cat
IPR013781Glyco_hydro_catalytic_dom
IPR017853Glycoside_hydrolase_SF