Pisum_sativum_v1_Contig2250

Contig Overview


Name	Pisum_sativum_v1_Contig2250
Unique Name	Pisum_sativum_v1_Contig2250
Type	contig
Organism	Pisum sativum (pea)
Sequence length	719

Unigenes

This contig is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	contig

Relationships

The following EST feature(s) are a part of this contig:


Feature Name	Unique Name	Species	Type
FG531255	FG531255	Pisum sativum	EST
EX569060	EX569060	Pisum sativum	EST
EX569061	EX569061	Pisum sativum	EST
FG533772	FG533772	Pisum sativum	EST
FG530863	FG530863	Pisum sativum	EST

Analyses

This contig is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
FG531255	EST	FG531255:71..441 +
EX569060	EST	EX569060:78..480 +
EX569061	EST	EX569061:1..623 +
FG533772	EST	FG533772:70..717 +
FG530863	EST	FG530863:71..719 +

Homology

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA2_ARATH (Nascent polypeptide-associated complex subunit alpha-like protein 2 OS=Arabidopsis thaliana GN=At3g49470 PE=2 SV=2)

HSP 1 Score: 164.466 bits (415), Expect = 5.335e-40
Identity = 91/163 (55.83%), Postives = 108/163 (66.26%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQG-AIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            +++D AQG +              AMLKLG+KPVTGVSRVTIKRTKN+LFFISKPDVFKSP+SETY+IFGEAKIED            F+MP++G+   + +   A   AQ                DIDLVMTQAGVSR+KAVKALK+H+GDIV AIMELTT
Sbjct:   55 EEDDDAQGVSGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNVLFFISKPDVFKSPHSETYVIFGEAKIEDLSSQLQTQAAQQFRMPEIGATSQRAEASTATVEAQVEEDEEEIDETGVEARDIDLVMTQAGVSRSKAVKALKSHDGDIVSAIMELTT 217

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA4_ARATH (Nascent polypeptide-associated complex subunit alpha-like protein 4 OS=Arabidopsis thaliana GN=At4g10480 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.521e-37
Identity = 87/139 (62.59%), Postives = 95/139 (68.35%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD-QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            AMLKLG+KPVT VSRVTIKR+KN+LF ISKPDVFKSPNSETY+IFGEAKI+D            FKMPD+ S++   D   A   AQ                DIDLVMTQAGVSR KAVKALK  NGDIV AIMELTT
Sbjct:   74 AMLKLGMKPVTDVSRVTIKRSKNVLFVISKPDVFKSPNSETYVIFGEAKIDDMSSQLQAQAAQRFKMPDVASMIPNTDGSEAATVAQEEEDDEDVDETGVEAKDIDLVMTQAGVSRPKAVKALKESNGDIVSAIMELTT 212

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA1_ARATH (Nascent polypeptide-associated complex subunit alpha-like protein 1 OS=Arabidopsis thaliana GN=At3g12390 PE=1 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 6.104e-36
Identity = 87/163 (53.37%), Postives = 103/163 (63.19%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXX-AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D+ DG  G A G             AMLKLG+KP+TGVSRVT+K++KNILF ISKPDVFKSP S+TY+IFGEAKIED            FK PD+ +V++K +  + A  Q              P DI+LVMTQAGVSR  AVKALK  +GDIV AIMELTT
Sbjct:   44 DEADGLDGEAGGKSKQSRSEKKSRKAMLKLGMKPITGVSRVTVKKSKNILFVISKPDVFKSPASDTYVIFGEAKIEDLSSQIQSQAAEQFKAPDLSNVISKGESSSAAVVQ---DDEEVDEEGVEPKDIELVMTQAGVSRPNAVKALKAADGDIVSAIMELTT 203

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA3_ARATH (Nascent polypeptide-associated complex subunit alpha-like protein 3 OS=Arabidopsis thaliana GN=At5g13850 PE=1 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 8.814e-35
Identity = 80/140 (57.14%), Postives = 97/140 (69.29%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD--QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            AMLKLG+KP+TGVSRVT+K++KNILF ISKPDVFKSP S+TY+IFGEAKIED            FK P++ +V+++ +    A A A               P DI+LVMTQAGVS+ +AVKALK  NGDIV AIMELTT
Sbjct:   65 AMLKLGMKPITGVSRVTVKKSKNILFVISKPDVFKSPASDTYVIFGEAKIEDLSSQLQSQAAEQFKAPNLSNVISQGETSSAATAAAVQDDDDEEVDEEGVEPKDIELVMTQAGVSKPRAVKALKLANGDIVSAIMELTT 204

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA_PINTA (Nascent polypeptide-associated complex subunit alpha-like protein OS=Pinus taeda PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.963e-34
Identity = 85/162 (52.47%), Postives = 96/162 (59.26%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D   G QGG               AMLKLG+KPV+GVSRVTIK++KNILF IS PDVFKSP S+TYI FGEAKIED            FK P++  V  K +    A                 P DI+LVMTQAGVSRTKAVKALK  +GDIV AIM+LTT
Sbjct:   48 DDAQGEQGGEGKSKQSRSEKKCRKAMLKLGMKPVSGVSRVTIKKSKNILFVISNPDVFKSPTSDTYIAFGEAKIEDLSSQLQTQAAEQFKAPNLSHVTMKPESSTAA----QEDEDEVDDTGVEPKDIELVMTQAGVSRTKAVKALKAADGDIVSAIMDLTT 205

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA5_ARATH (Nascent polypeptide-associated complex subunit alpha-like protein 5 OS=Arabidopsis thaliana GN=At1g33040 PE=2 SV=2)

HSP 1 Score: 144.436 bits (363), Expect = 5.713e-34
Identity = 81/163 (49.69%), Postives = 99/163 (60.74%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQ-DQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D E   +GG E             A+LKLG+KPV+ VSRVTIKR KN+LF ISKPDV+KSPN+ETY+IFGEAK++D            FKMPD+ S++     +  +A                   DIDLVMTQAGVS+ KAV ALK ++GDIV AIMELTT
Sbjct:   47 DVEVEGEGGNENAKQSRSEKKSRKAVLKLGMKPVSDVSRVTIKRAKNVLFVISKPDVYKSPNAETYVIFGEAKVDDLSSQLQTQAAQRFKMPDVTSMLPNAGSEATMAPLAEEEDEDDVDDTGVEARDIDLVMTQAGVSKAKAVSALKANDGDIVSAIMELTT 209

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA_ORENI (Nascent polypeptide-associated complex subunit alpha OS=Oreochromis niloticus GN=naca PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.363e-27
Identity = 71/137 (51.82%), Postives = 87/137 (63.50%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            AM KLGL+ VTGV+RVTI+++KNILF I+KPDV+KSP S+TYI+FGEAKIED            FK+P   +V   Q+       Q                DI+LVM+QA VSR KAV+ALK +N DIV AIMELT
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVPG-ETVSNVQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 214

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA_DROME (Nascent polypeptide-associated complex subunit alpha OS=Drosophila melanogaster GN=Nacalpha PE=1 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 2.570e-26
Identity = 67/138 (48.55%), Postives = 84/138 (60.87%), Query Frame = 1

Query:   76 MLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQP--XXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            MLKLGLK + GV+RVTI+++KNILF I+ PDV+K+P+S+TYI+FGEAKIED            FK P+     A    GA     P                 DI+LV+TQA  +R KA+KALK +N DIV AIMELT
Sbjct:   80 MLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAG--AADSVGATTSVAPIAEEDEEDVDDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELT 215

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA_XENTR (Nascent polypeptide-associated complex subunit alpha OS=Xenopus tropicalis GN=naca PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 5.726e-26
Identity = 69/137 (50.36%), Postives = 86/137 (62.77%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            AM KLGL+ VTGV+RVTI+++KNILF I+KPDV+KSP S+TYI+FGEAKIED            FK+    +V   Q+       Q                DI+LVM+QA VSR KAV+ALK ++ DIV AIMELT
Sbjct:   78 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQG-EAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 213

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Match: NACA_XENLA (Nascent polypeptide-associated complex subunit alpha OS=Xenopus laevis GN=naca PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 5.726e-26
Identity = 69/137 (50.36%), Postives = 86/137 (62.77%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            AM KLGL+ VTGV+RVTI+++KNILF I+KPDV+KSP S+TYI+FGEAKIED            FK+    +V   Q+       Q                DI+LVM+QA VSR KAV+ALK ++ DIV AIMELT
Sbjct:   77 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQG-ETVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 212

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: C6SYH0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 2.568e-53
Identity = 117/163 (71.78%), Postives = 120/163 (73.62%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQ-GAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            DKED AQGGAEG            AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIED            F MPD+GSV+AKQDQ  A A AQP             PHDIDLVMTQAGVSR+KAVKALKTHNGDIVGAIMELTT
Sbjct:   63 DKEDDAQGGAEGGKQSRSEKKSRKAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDLSSQLQTQAAQQFGMPDVGSVLAKQDQDAAAAAAQPEEEEEEVDETGVEPHDIDLVMTQAGVSRSKAVKALKTHNGDIVGAIMELTT 225

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: C6SY77_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 7.471e-53
Identity = 115/162 (70.99%), Postives = 119/162 (73.46%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            DKED AQGGAEG            AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETY+IFGEAKIED            F+MPDMGSV AKQ+  A A AQP             PHDIDLVMTQAGVSR+KAVKALKTHNGDIVGAIMELTT
Sbjct:   62 DKEDDAQGGAEGSKQSRSEKKSRKAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMGSVTAKQEDAAAAAAQP-EEEEEVDETGVEPHDIDLVMTQAGVSRSKAVKALKTHNGDIVGAIMELTT 222

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: C6TI89_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 4.842e-52
Identity = 114/162 (70.37%), Postives = 118/162 (72.84%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            DKED AQGGAEG            AMLK GLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETY+IFGEAKIED            F+MPDMGSV AKQ+  A A AQP             PHDIDLVMTQAGVSR+KAVKALKTHNGDIVGAIMELTT
Sbjct:   63 DKEDDAQGGAEGSKQSRSEKKSRKAMLKPGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMGSVTAKQEDAAAAAAQP-EEEEEVDETGVEPHDIDLVMTQAGVSRSKAVKALKTHNGDIVGAIMELTT 223

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: B7FMW4_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 1.840e-51
Identity = 116/163 (71.17%), Postives = 120/163 (73.62%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIA-GAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            DKED A  GA+G            AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIED            F+MPDMGSVMAKQDQGA A GA P             PHDIDLVMTQAGVS++KAVKALKTHNGDIVGAIMELTT
Sbjct:   66 DKED-ALAGADGSKQSRSEKKSRKAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDLSSQLQTQAAQQFRMPDMGSVMAKQDQGASASGAIPEEEEEEIDETGVEPHDIDLVMTQAGVSKSKAVKALKTHNGDIVGAIMELTT 227

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: B9S5M4_RICCO (Nascent polypeptide associated complex alpha subunit, putative OS=Ricinus communis GN=RCOM_0755870 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 2.845e-44
Identity = 102/163 (62.58%), Postives = 108/163 (66.26%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD-QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            DKE G  G                AMLKLG+KPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETY+IFGEAKIED            F+MPDM SV+ K D  GA A AQ              P DIDLVMTQAGV R+KAVKALKTH+GDIV AIMELTT
Sbjct:   66 DKEAGTPGANGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMASVLPKSDLSGAAAAAQADEEEEEVDETGVEPRDIDLVMTQAGVPRSKAVKALKTHSGDIVSAIMELTT 228

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: B9IDG2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_574643 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 9.154e-43
Identity = 99/163 (60.74%), Postives = 107/163 (65.64%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQG-AIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D +D   G                AMLKLG+KPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETY+IFGEAKIED            F+MPDM S++ K D   A A A P             P DIDLVMTQAGVSRTKAVKAL+T+NGDIV AIMELTT
Sbjct:   63 DDDDDTPGANGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMSSMLPKSDASTAAASAPPEEEEEEVDDTGVEPRDIDLVMTQAGVSRTKAVKALQTNNGDIVSAIMELTT 225

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: A9PB21_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 9.154e-43
Identity = 99/163 (60.74%), Postives = 107/163 (65.64%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQG-AIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D +D   G                AMLKLG+KPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETY+IFGEAKIED            F+MPDM S++ K D   A A A P             P DIDLVMTQAGVSRTKAVKAL+T+NGDIV AIMELTT
Sbjct:   62 DDDDDTPGANGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMSSMLPKSDASTAAASAPPEEEEEEVDDTGVEPRDIDLVMTQAGVSRTKAVKALQTNNGDIVSAIMELTT 224

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: B6SU89_MAIZE (Nascent polypeptide-associated complex alpha subunit-like protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 3.256e-40
Identity = 92/161 (57.14%), Postives = 102/161 (63.35%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            D  DG  G  EG            AM+KLG+KPVTGV+R+TIKR KNILF +SKPDVFKSP SETY+IFGEAKIED            F+M D+  VMAKQD  A A A P             P DIDLVMTQA V+R KAVKALK H+GDIV AIMELT
Sbjct:   68 DDADGGAGATEGTKQSRSEKKSRKAMMKLGMKPVTGVTRITIKRAKNILFVVSKPDVFKSPTSETYVIFGEAKIEDLSSQLQAQAAQQFRMQDLSKVMAKQD-AAAAAAAPADEEEEVDEAGIEPRDIDLVMTQASVTRAKAVKALKAHDGDIVSAIMELT 227

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: Q8LMR3_ORYSJ (Nascent polypeptide-associated complex alpha subunit, putative, expressed OS=Oryza sativa subsp. japonica GN=OJ1705B08.10 PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 6.140e-39
Identity = 93/163 (57.06%), Postives = 103/163 (63.19%), Query Frame = 1

Query:    1 DKEDGAQG--GAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            + EDG QG  G EG            AM+KLG+KPVTGVSR+TIKR KNILF +SKPDVFKSP SETY+IFGEAKIED            F+M D+  VM+K D    A A P             P DIDLVMTQA VSR KAVKALK H+GDIV AIMELT
Sbjct:   61 EDEDGEQGAIGNEGSKQSRSEKKSRKAMMKLGMKPVTGVSRITIKRAKNILFVVSKPDVFKSPTSETYVIFGEAKIEDLSSQLQAQAAQQFRMQDLSKVMSKPD---AAAAAPADEEEEVDETGIEPRDIDLVMTQASVSRAKAVKALKAHDGDIVSAIMELT 220

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Match: A2XBV9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09778 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 6.140e-39
Identity = 93/163 (57.06%), Postives = 103/163 (63.19%), Query Frame = 1

Query:    1 DKEDGAQG--GAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELT 483
            + EDG QG  G EG            AM+KLG+KPVTGVSR+TIKR KNILF +SKPDVFKSP SETY+IFGEAKIED            F+M D+  VM+K D    A A P             P DIDLVMTQA VSR KAVKALK H+GDIV AIMELT
Sbjct:   61 EDEDGEQGAIGNEGSKQSRSEKKSRKAMMKLGMKPVTGVSRITIKRAKNILFVVSKPDVFKSPTSETYVIFGEAKIEDLSSQLQAQAAQQFRMQDLSKVMSKPD---AAAAAPADEEEEVDETGIEPRDIDLVMTQASVSRAKAVKALKAHDGDIVSAIMELT 220

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT3G49470.1 (| Symbols: NACA2 | nascent polypeptide-associated complex subunit alpha-like protein 2 | chr3:18341072-18342273 FORWARD LENGTH=217)

HSP 1 Score: 164.466 bits (415), Expect = 5.398e-41
Identity = 91/163 (55.83%), Postives = 108/163 (66.26%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQG-AIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            +++D AQG +              AMLKLG+KPVTGVSRVTIKRTKN+LFFISKPDVFKSP+SETY+IFGEAKIED            F+MP++G+   + +   A   AQ                DIDLVMTQAGVSR+KAVKALK+H+GDIV AIMELTT
Sbjct:   55 EEDDDAQGVSGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNVLFFISKPDVFKSPHSETYVIFGEAKIEDLSSQLQTQAAQQFRMPEIGATSQRAEASTATVEAQVEEDEEEIDETGVEARDIDLVMTQAGVSRSKAVKALKSHDGDIVSAIMELTT 217

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT4G10480.2 (| Symbols: | Nascent polypeptide-associated complex (NAC), alpha subunit family protein | chr4:6478089-6479079 REVERSE LENGTH=211)

HSP 1 Score: 154.451 bits (389), Expect = 5.586e-38
Identity = 87/139 (62.59%), Postives = 95/139 (68.35%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD-QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            AMLKLG+KPVT VSRVTIKR+KN+LF ISKPDVFKSPNSETY+IFGEAKI+D            FKMPD+ S++   D   A   AQ                DIDLVMTQAGVSR KAVKALK  NGDIV AIMELTT
Sbjct:   73 AMLKLGMKPVTDVSRVTIKRSKNVLFVISKPDVFKSPNSETYVIFGEAKIDDMSSQLQAQAAQRFKMPDVASMIPNTDGSEAATVAQEEEDDEDVDETGVEAKDIDLVMTQAGVSRPKAVKALKESNGDIVSAIMELTT 211

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT4G10480.1 (| Symbols: | Nascent polypeptide-associated complex (NAC), alpha subunit family protein | chr4:6478089-6479079 REVERSE LENGTH=212)

HSP 1 Score: 154.451 bits (389), Expect = 5.586e-38
Identity = 87/139 (62.59%), Postives = 95/139 (68.35%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD-QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            AMLKLG+KPVT VSRVTIKR+KN+LF ISKPDVFKSPNSETY+IFGEAKI+D            FKMPD+ S++   D   A   AQ                DIDLVMTQAGVSR KAVKALK  NGDIV AIMELTT
Sbjct:   74 AMLKLGMKPVTDVSRVTIKRSKNVLFVISKPDVFKSPNSETYVIFGEAKIDDMSSQLQAQAAQRFKMPDVASMIPNTDGSEAATVAQEEEDDEDVDETGVEAKDIDLVMTQAGVSRPKAVKALKESNGDIVSAIMELTT 212

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT3G12390.1 (| Symbols: | Nascent polypeptide-associated complex (NAC), alpha subunit family protein | chr3:3942344-3943595 FORWARD LENGTH=203)

HSP 1 Score: 150.984 bits (380), Expect = 6.176e-37
Identity = 87/163 (53.37%), Postives = 103/163 (63.19%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXX-AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQDQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D+ DG  G A G             AMLKLG+KP+TGVSRVT+K++KNILF ISKPDVFKSP S+TY+IFGEAKIED            FK PD+ +V++K +  + A  Q              P DI+LVMTQAGVSR  AVKALK  +GDIV AIMELTT
Sbjct:   44 DEADGLDGEAGGKSKQSRSEKKSRKAMLKLGMKPITGVSRVTVKKSKNILFVISKPDVFKSPASDTYVIFGEAKIEDLSSQIQSQAAEQFKAPDLSNVISKGESSSAAVVQ---DDEEVDEEGVEPKDIELVMTQAGVSRPNAVKALKAADGDIVSAIMELTT 203

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT5G13850.1 (| Symbols: NACA3 | nascent polypeptide-associated complex subunit alpha-like protein 3 | chr5:4471361-4472676 FORWARD LENGTH=204)

HSP 1 Score: 147.132 bits (370), Expect = 8.918e-36
Identity = 80/140 (57.14%), Postives = 97/140 (69.29%), Query Frame = 1

Query:   73 AMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQD--QGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            AMLKLG+KP+TGVSRVT+K++KNILF ISKPDVFKSP S+TY+IFGEAKIED            FK P++ +V+++ +    A A A               P DI+LVMTQAGVS+ +AVKALK  NGDIV AIMELTT
Sbjct:   65 AMLKLGMKPITGVSRVTVKKSKNILFVISKPDVFKSPASDTYVIFGEAKIEDLSSQLQSQAAEQFKAPNLSNVISQGETSSAATAAAVQDDDDEEVDEEGVEPKDIELVMTQAGVSKPRAVKALKLANGDIVSAIMELTT 204

[Close]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Match: AT1G33040.1 (| Symbols: NACA5 | nascent polypeptide-associated complex subunit alpha-like protein 5 | chr1:11966547-11967696 FORWARD LENGTH=209)

HSP 1 Score: 144.436 bits (363), Expect = 5.780e-35
Identity = 81/163 (49.69%), Postives = 99/163 (60.74%), Query Frame = 1

Query:    1 DKEDGAQGGAEGXXXXXXXXXXXXAMLKLGLKPVTGVSRVTIKRTKNILFFISKPDVFKSPNSETYIIFGEAKIEDXXXXXXXXXXXXFKMPDMGSVMAKQ-DQGAIAGAQPXXXXXXXXXXXXXPHDIDLVMTQAGVSRTKAVKALKTHNGDIVGAIMELTT 486
            D E   +GG E             A+LKLG+KPV+ VSRVTIKR KN+LF ISKPDV+KSPN+ETY+IFGEAK++D            FKMPD+ S++     +  +A                   DIDLVMTQAGVS+ KAV ALK ++GDIV AIMELTT
Sbjct:   47 DVEVEGEGGNENAKQSRSEKKSRKAVLKLGMKPVSDVSRVTIKRAKNVLFVISKPDVYKSPNAETYVIFGEAKVDDLSSQLQTQAAQRFKMPDVTSMLPNAGSEATMAPLAEEEDEDDVDDTGVEARDIDLVMTQAGVSKAKAVSALKANDGDIVSAIMELTT 209

The following BLAST results are available for this feature:

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide

BLAST of Pisum_sativum_v1_Contig2250 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NACA2_ARATH	5.335e-40	55.83	Nascent polypeptide-associated complex subunit alp...	[more]
NACA4_ARATH	5.521e-37	62.59	Nascent polypeptide-associated complex subunit alp...	[more]
NACA1_ARATH	6.104e-36	53.37	Nascent polypeptide-associated complex subunit alp...	[more]
NACA3_ARATH	8.814e-35	57.14	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_PINTA	1.963e-34	52.47	Nascent polypeptide-associated complex subunit alp...	[more]
NACA5_ARATH	5.713e-34	49.69	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_ORENI	1.363e-27	51.82	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_DROME	2.570e-26	48.55	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_XENTR	5.726e-26	50.36	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_XENLA	5.726e-26	50.36	Nascent polypeptide-associated complex subunit alp...	[more]

BLAST of Pisum_sativum_v1_Contig2250 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SYH0_SOYBN	2.568e-53	71.78	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SY77_SOYBN	7.471e-53	70.99	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TI89_SOYBN	4.842e-52	70.37	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FMW4_MEDTR	1.840e-51	71.17	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9S5M4_RICCO	2.845e-44	62.58	Nascent polypeptide associated complex alpha subun...	[more]
B9IDG2_POPTR	9.154e-43	60.74	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PB21_POPTR	9.154e-43	60.74	Putative uncharacterized protein OS=Populus tricho...	[more]
B6SU89_MAIZE	3.256e-40	57.14	Nascent polypeptide-associated complex alpha subun...	[more]
Q8LMR3_ORYSJ	6.140e-39	57.06	Nascent polypeptide-associated complex alpha subun...	[more]
A2XBV9_ORYSI	6.140e-39	57.06	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of Pisum_sativum_v1_Contig2250 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G49470.1	5.398e-41	55.83	\| Symbols: NACA2 \| nascent polypeptide-associated ...	[more]
AT4G10480.2	5.586e-38	62.59	\| Symbols: \| Nascent polypeptide-associated compl...	[more]
AT4G10480.1	5.586e-38	62.59	\| Symbols: \| Nascent polypeptide-associated compl...	[more]
AT3G12390.1	6.176e-37	53.37	\| Symbols: \| Nascent polypeptide-associated compl...	[more]
AT5G13850.1	8.918e-36	57.14	\| Symbols: NACA3 \| nascent polypeptide-associated ...	[more]
AT1G33040.1	5.780e-35	49.69	\| Symbols: NACA5 \| nascent polypeptide-associated ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000449	Ubiquitin-associated/translation elongation factor EF1B, N-terminal	PFAM	PF00627	UBA	coord: 126..160 score: 0.0
IPR002715	Nascent polypeptide-associated complex NAC	PFAM	PF01849	NAC	coord: 19..76 score: 3.2
IPR002715	Nascent polypeptide-associated complex NAC	PROFILE	PS51151	NAC_AB	coord: 16..81 score: 17
IPR016641	Nascent polypeptide-associated complex, alpha subunit	PIR	PIRSF015901	Nascent polypeptide-associated complex, alpha subunit	coord: 1..162 score: 8.4
None	No IPR available	SEG	seg	seg	coord: 13..24 score: NAcoord: 77..88 score: NAcoord: 112..124 score
None	No IPR available	SEG	seg	seg	coord: 34..53 score
None	No IPR available	SEG	seg	seg	coord: 83..93 score

Sequences

The following sequences are available for this feature:

contig sequence

contig sequence

>Pisum_sativum_v1_Contig2250 ID=Pisum_sativum_v1_Contig2250; Name=Pisum_sativum_v1_Contig2250; organism=Pisum sativum; type=contig; length=719bp
GACAAGGAAGATGGCGCTCAAGGTGGTGCTGAGGGTTCAAAGCAAAGCAG
AAGCGAGAAGAAGAGTCGAAAAGCAATGCTGAAGCTCGGACTGAAACCTG
TTACTGGCGTTAGTCGGGTCACAATCAAGAGAACCAAAAATATTCTTTTT
TTCATCTCAAAACCCGATGTATTCAAGAGTCCAAATTCTGAGACCTACAT
TATATTCGGGGAAGCAAAAATAGAAGACCTGAGCTCTCAGTTGCAAACTC
AAGCTGCTCAGCAGTTTAAGATGCCAGATATGGGATCTGTAATGGCAAAA
CAAGATCAAGGTGCAATAGCTGGTGCACAGCCTGAGGAGGAAGAAGAGGA
GGTCGATGAAACTGGCGTGGAGCCCCATGACATTGATTTGGTCATGACAC
AAGCAGGAGTTTCCCGGACCAAGGCCGTCAAGGCTCTCAAGACTCACAAT
GGAGACATTGTTGGTGCCATTATGGAGCTCACTACTTAATTGGGCTTTGT
TTAATTGGGCTGTGTTCTATTTGTATTTTTTTGTGTGGAATTATTTTTGA
ACTAGTAGTTTCTTTAAGTTGCTGTAGCTACAGACCATATCACCTTATCA
AGCGTCTATATGTCTAGGTTTACCATTTGCCTGATAATTTTATTAAGTTT
TGTGTTTCCTAGCAATCAAGGAGTGACATTCAGATGCTTTTTTGTTACAG
AATGATCACATGTTTGTCT

Annotated Terms

The following terms have been associated with this contig:

Vocabulary: **INTERPRO**
Term	Definition
IPR000449	Ubiquitin-associated/translation elongation factor EF1B, N-terminal
IPR002715	Nas_poly-pep-assoc_cplx_dom
IPR016641	EGD2/NACA

Search form

Pisum_sativum_v1_Contig2250