GT628078

EST Overview


Name	GT628078
Unique Name	GT628078
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	517

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT628078 vs. TrEMBL
Match: Q9AUZ8_ORYSJ (Putative uncharacterized protein OSJNBa0026O12.1 OS=Oryza sativa subsp. japonica GN=OSJNBa0026O12.1 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.019e-23
Identity = 49/57 (85.96%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQERE+P +TEWDRFAHREYIRLSMEEDGED SN SGDVW+ESLEAPF
Sbjct:  522 FETRDPFLIRQERENPTLTEWDRFAHREYIRLSMEEDGEDASNGSGDVWDESLEAPF 578

[Close]

BLAST of GT628078 vs. TrEMBL
Match: Q7XDE0_ORYSJ (EF hand family protein, expressed OS=Oryza sativa subsp. japonica GN=Os10g0476600 PE=2 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 6.019e-23
Identity = 49/57 (85.96%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQERE+P +TEWDRFAHREYIRLSMEEDGED SN SGDVW+ESLEAPF
Sbjct:  485 FETRDPFLIRQERENPTLTEWDRFAHREYIRLSMEEDGEDASNGSGDVWDESLEAPF 541

[Close]

BLAST of GT628078 vs. TrEMBL
Match: C5WYG5_SORBI (Putative uncharacterized protein Sb01g019370 OS=Sorghum bicolor GN=Sb01g019370 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.019e-23
Identity = 49/57 (85.96%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQERE+P +TEWDRFAHREYIRLSMEEDGED SN SGDVW+ESLEAPF
Sbjct:  485 FETRDPFLIRQERENPTLTEWDRFAHREYIRLSMEEDGEDASNGSGDVWDESLEAPF 541

[Close]

BLAST of GT628078 vs. TrEMBL
Match: B8BH78_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_34023 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.019e-23
Identity = 49/57 (85.96%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQERE+P +TEWDRFAHREYIRLSMEEDGED SN SGDVW+ESLEAPF
Sbjct:  405 FETRDPFLIRQERENPTLTEWDRFAHREYIRLSMEEDGEDASNGSGDVWDESLEAPF 461

[Close]

BLAST of GT628078 vs. TrEMBL
Match: D7U325_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028366001 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.341e-22
Identity = 48/57 (84.21%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQEREDP +TEWDRFAHREYIRLSMEEDGED+SN S +VW+ESLEAPF
Sbjct:  491 FETRDPFLIRQEREDPTLTEWDRFAHREYIRLSMEEDGEDVSNGSAEVWDESLEAPF 547

[Close]

BLAST of GT628078 vs. TrEMBL
Match: B9SV22_RICCO (Calcium ion binding protein, putative OS=Ricinus communis GN=RCOM_0259220 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.095e-22
Identity = 49/57 (85.96%), Postives = 53/57 (92.98%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FESRDP+LIRQEREDP +TEWDRFAHREYIRLSMEED ED+SN S DVW+ESLEAPF
Sbjct:  489 FESRDPFLIRQEREDPTLTEWDRFAHREYIRLSMEEDVEDVSNGSVDVWDESLEAPF 545

[Close]

BLAST of GT628078 vs. TrEMBL
Match: B8LS08_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.095e-22
Identity = 47/57 (82.46%), Postives = 53/57 (92.98%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQEREDP +TEWDRFAH+EYIRLSMEEDGED SN S +VW+ESLEAPF
Sbjct:  490 FETRDPFLIRQEREDPTLTEWDRFAHKEYIRLSMEEDGEDASNGSAEVWDESLEAPF 546

[Close]

BLAST of GT628078 vs. TrEMBL
Match: B9H8Q2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_818733 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.634e-21
Identity = 46/57 (80.70%), Postives = 52/57 (91.23%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FESRDP+LIRQERE+P +TEWDRFAHREYIRLSMEED ED+SN S +VW+ES EAPF
Sbjct:  485 FESRDPFLIRQEREEPTLTEWDRFAHREYIRLSMEEDVEDVSNGSAEVWDESFEAPF 541

[Close]

BLAST of GT628078 vs. TrEMBL
Match: Q69M01_ORYSJ (Putative uncharacterized protein P0453H10.11 OS=Oryza sativa subsp. japonica GN=P0453H10.11 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 7.358e-21
Identity = 45/57 (78.95%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQ RE+P++T+WDRFA REYIRL+MEEDGED SNASGDVW+ESLE+PF
Sbjct:  496 FEARDPFLIRQMREEPSLTDWDRFARREYIRLAMEEDGEDASNASGDVWDESLESPF 552

[Close]

BLAST of GT628078 vs. TrEMBL
Match: Q0E365_ORYSJ (Os02g0191700 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os02g0191700 PE=4 SV=2)

HSP 1 Score: 103.605 bits (257), Expect = 7.358e-21
Identity = 45/57 (78.95%), Postives = 54/57 (94.74%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQ RE+P++T+WDRFA REYIRL+MEEDGED SNASGDVW+ESLE+PF
Sbjct:   80 FEARDPFLIRQMREEPSLTDWDRFARREYIRLAMEEDGEDASNASGDVWDESLESPF 136

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT1G03960.1 (| Symbols: | Calcium-binding EF hand family protein | chr1:1014085-1017472 FORWARD LENGTH=529)

HSP 1 Score: 98.2117 bits (243), Expect = 2.677e-21
Identity = 43/57 (75.44%), Postives = 50/57 (87.72%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQEREDPN+TEWDRFA REY RLSMEED +++SN S DVW+E LE PF
Sbjct:  473 FETRDPFLIRQEREDPNLTEWDRFAQREYARLSMEEDVDEVSNGSADVWDEPLEPPF 529

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT1G03960.2 (| Symbols: | Calcium-binding EF hand family protein | chr1:1014824-1017472 FORWARD LENGTH=389)

HSP 1 Score: 98.2117 bits (243), Expect = 2.677e-21
Identity = 43/57 (75.44%), Postives = 50/57 (87.72%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQEREDPN+TEWDRFA REY RLSMEED +++SN S DVW+E LE PF
Sbjct:  333 FETRDPFLIRQEREDPNLTEWDRFAQREYARLSMEEDVDEVSNGSADVWDEPLEPPF 389

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT5G28900.1 (| Symbols: | Calcium-binding EF-hand family protein | chr5:10925852-10929892 FORWARD LENGTH=536)

HSP 1 Score: 97.4413 bits (241), Expect = 4.566e-21
Identity = 45/58 (77.59%), Postives = 52/58 (89.66%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEE-SLEAPF 172
            FE+RDP+LIRQERE+P +T+WDRFAHREYIRLSMEED ED SN S +VW+E SLEAPF
Sbjct:  479 FETRDPFLIRQERENPTLTDWDRFAHREYIRLSMEEDVEDASNGSAEVWDESSLEAPF 536

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT5G44090.1 (| Symbols: | Calcium-binding EF-hand family protein | chr5:17743264-17747909 REVERSE LENGTH=538)

HSP 1 Score: 97.4413 bits (241), Expect = 4.566e-21
Identity = 42/57 (73.68%), Postives = 51/57 (89.47%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQERE+P +TEWDRFA REY+RLSMEED E++SN S DVW+E LE+PF
Sbjct:  482 FETRDPFLIRQERENPTLTEWDRFAQREYVRLSMEEDVEEVSNGSADVWDEPLESPF 538

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT5G28850.2 (| Symbols: | Calcium-binding EF-hand family protein | chr5:10877360-10881278 REVERSE LENGTH=536)

HSP 1 Score: 96.2857 bits (238), Expect = 1.017e-20
Identity = 44/58 (75.86%), Postives = 52/58 (89.66%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEE-SLEAPF 172
            FE+RDP+LIRQERE+P +T+WDRFAHREYIRLSMEED ED SN S +VW++ SLEAPF
Sbjct:  479 FETRDPFLIRQERENPTLTDWDRFAHREYIRLSMEEDVEDASNGSAEVWDDSSLEAPF 536

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT5G28850.1 (| Symbols: | Calcium-binding EF-hand family protein | chr5:10877360-10879416 REVERSE LENGTH=324)

HSP 1 Score: 96.2857 bits (238), Expect = 1.017e-20
Identity = 44/58 (75.86%), Postives = 52/58 (89.66%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEE-SLEAPF 172
            FE+RDP+LIRQERE+P +T+WDRFAHREYIRLSMEED ED SN S +VW++ SLEAPF
Sbjct:  267 FETRDPFLIRQERENPTLTDWDRFAHREYIRLSMEEDVEDASNGSAEVWDDSSLEAPF 324

[Close]

BLAST of GT628078 vs. TAIR peptide
Match: AT1G54450.1 (| Symbols: | Calcium-binding EF-hand family protein | chr1:20336570-20339535 REVERSE LENGTH=535)

HSP 1 Score: 95.5153 bits (236), Expect = 1.735e-20
Identity = 42/57 (73.68%), Postives = 48/57 (84.21%), Query Frame = 2

Query:    2 FESRDPYLIRQEREDPNMTEWDRFAHREYIRLSMEEDGEDMSNASGDVWEESLEAPF 172
            FE+RDP+LIRQER +P  TEWDRFAHREYIRLSMEED ED SN S + W++SLE PF
Sbjct:  479 FETRDPFLIRQERANPTWTEWDRFAHREYIRLSMEEDVEDASNGSAEAWDDSLEVPF 535

The following BLAST results are available for this feature:

BLAST of GT628078 vs. TrEMBL
BLAST of GT628078 vs. TAIR peptide

BLAST of GT628078 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9AUZ8_ORYSJ	6.019e-23	85.96	Putative uncharacterized protein OSJNBa0026O12.1 O...	[more]
Q7XDE0_ORYSJ	6.019e-23	85.96	EF hand family protein, expressed OS=Oryza sativa ...	[more]
C5WYG5_SORBI	6.019e-23	85.96	Putative uncharacterized protein Sb01g019370 OS=So...	[more]
B8BH78_ORYSI	6.019e-23	85.96	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7U325_VITVI	1.341e-22	84.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SV22_RICCO	5.095e-22	85.96	Calcium ion binding protein, putative OS=Ricinus c...	[more]
B8LS08_PICSI	5.095e-22	82.46	Putative uncharacterized protein OS=Picea sitchens...	[more]
B9H8Q2_POPTR	5.634e-21	80.70	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q69M01_ORYSJ	7.358e-21	78.95	Putative uncharacterized protein P0453H10.11 OS=Or...	[more]
Q0E365_ORYSJ	7.358e-21	78.95	Os02g0191700 protein (Fragment) OS=Oryza sativa su...	[more]

BLAST of GT628078 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G03960.1	2.677e-21	75.44	\| Symbols: \| Calcium-binding EF hand family prote...	[more]
AT1G03960.2	2.677e-21	75.44	\| Symbols: \| Calcium-binding EF hand family prote...	[more]
AT5G28900.1	4.566e-21	77.59	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT5G44090.1	4.566e-21	73.68	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT5G28850.2	1.017e-20	75.86	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT5G28850.1	1.017e-20	75.86	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT1G54450.1	1.735e-20	73.68	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 27..40 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT628078 ID=GT628078; Name=GT628078; organism=Lens culinaris; type=EST; length=517bp
GTTTGAATCTCGTGATCCGTATCTTATACGTCAGGAACGCGAGGATCCAA
ATATGACAGAGTGGGACCGTTTTGCACACAGGGAGTATATTAGGCTTTCT
ATGGAAGAAGATGGTGAAGACATGTCAAATGCCAGTGGAGATGTCTGGGA
AGAATCACTTGAGGCCCCTTTCTGAAATTCAATTAGGTGAATCAAAGGCT
AAGTTGTGCATGTAAGAATCTTCACACTCTACAACTCGAACATGGATGCA
TTTGGAGGTATATGTCTAATAGTGTTCTGGAGAGGTTCTCCTGAAATTTG
TTTAGAAAGAAAATGAGAGTAGCTACAGGGTAAGAGCTGGTGCAGATTTA
TTGTTCTTGTTCATATACTAGTACTTATTTTTTAGACTAATCTATATCAG
AGTTTAATTTCTGTAAAAAGAAACCGGAGGGTTTTTGGTTTATCCCTTTT
GAATTTTGGTTGTTTGAAGGTTTTGATTTTTTTACAAAATTTATCTCAAT
TAATTGTACTTAACTTT

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