GT623421

EST Overview


Name	GT623421
Unique Name	GT623421
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	362

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT623421 vs. TrEMBL
Match: B9H2I1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555677 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.456e-12
Identity = 37/56 (66.07%), Postives = 45/56 (80.36%), Query Frame = 3

Query:    9 GHETDEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            G + DE++DFL+QIRTKSF LR T   K + S+GPTAN +V+AILEKANAIRQ VA
Sbjct:  231 GKDIDEKDDFLHQIRTKSFTLRRTGKAKPSLSSGPTANNKVSAILEKANAIRQAVA 286

[Close]

BLAST of GT623421 vs. TrEMBL
Match: B9I1L8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_728200 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.700e-11
Identity = 38/57 (66.67%), Postives = 45/57 (78.95%), Query Frame = 3

Query:    9 GHETDEREDFLYQIRTKSFNLRPTVTGKSNT-STGPTANVQVTAILEKANAIRQVVA 176
            G + DE+EDFL+QIRTKSF LR T T K  T  +GPTA+ +V+AILEKANAIRQ VA
Sbjct:  868 GKDIDEKEDFLHQIRTKSFTLRRTATAKPTTLFSGPTASNKVSAILEKANAIRQAVA 924

[Close]

BLAST of GT623421 vs. TrEMBL
Match: D7TJL3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021213001 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.398e-10
Identity = 36/55 (65.45%), Postives = 39/55 (70.91%), Query Frame = 3

Query:    9 GHETDEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVV 173
            G + DEREDFL QIRTKSF+LR T T +      P  NV VTAILEKANAIRQ V
Sbjct:  773 GKKMDEREDFLEQIRTKSFSLRRTATPRLTVMPTPATNVSVTAILEKANAIRQAV 827

[Close]

BLAST of GT623421 vs. TrEMBL
Match: C7IWQ9_ORYSJ (Os01g0208600 protein OS=Oryza sativa subsp. japonica GN=Os01g0208600 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.343e-10
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 3

Query:    6 MGHET---DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            +G++T   DERE+ L QIR+K+FNLR T   K+NTS+  TAN  V AILEKANAIRQ VA
Sbjct: 1262 VGNDTKNLDEREELLQQIRSKTFNLRRTNASKTNTSSPTTANSSVVAILEKANAIRQAVA 1321

[Close]

BLAST of GT623421 vs. TrEMBL
Match: B8ACP1_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00845 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.343e-10
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 3

Query:    6 MGHET---DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            +G++T   DERE+ L QIR+K+FNLR T   K+NTS+  TAN  V AILEKANAIRQ VA
Sbjct:  766 VGNDTKNLDEREELLQQIRSKTFNLRRTNASKTNTSSPTTANSSVVAILEKANAIRQAVA 825

[Close]

BLAST of GT623421 vs. TrEMBL
Match: A2ZQJ1_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_00831 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.343e-10
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 3

Query:    6 MGHET---DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            +G++T   DERE+ L QIR+K+FNLR T   K+NTS+  TAN  V AILEKANAIRQ VA
Sbjct: 1164 VGNDTKNLDEREELLQQIRSKTFNLRRTNASKTNTSSPTTANSSVVAILEKANAIRQAVA 1223

[Close]

BLAST of GT623421 vs. TrEMBL
Match: Q7XXS7_ORYSJ (Putative uncharacterized protein (Fragment) OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.190e-9
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 3

Query:    6 MGHET---DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            +G++T   DERE+ L QIR+K+FNLR T   K+NTS+  TAN  V AILEKANAIRQ VA
Sbjct:  191 VGNDTKNLDEREELLQQIRSKTFNLRRTNAFKTNTSSPTTANSSVVAILEKANAIRQAVA 250

[Close]

BLAST of GT623421 vs. TrEMBL
Match: B9S9B2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1015180 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.030e-9
Identity = 34/51 (66.67%), Postives = 38/51 (74.51%), Query Frame = 3

Query:   21 DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVV 173
            DE+EDFL+QIR KSF LR TV  K   + GP AN +VTAILEKA AIRQ V
Sbjct: 1047 DEKEDFLHQIRRKSFTLRRTVAAKPTFAAGPAANDKVTAILEKAIAIRQAV 1097

[Close]

BLAST of GT623421 vs. TrEMBL
Match: B9SL38_RICCO (Protein SCAR2, putative OS=Ricinus communis GN=RCOM_0850090 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.531e-8
Identity = 31/59 (52.54%), Postives = 41/59 (69.49%), Query Frame = 3

Query:    3 KMGHETDEREDFLYQIRTKSFNLRPTVTGKSNTS--TGPTANVQVTAILEKANAIRQVV 173
            ++G + DER+  L QIRTKSFNL+PT   + +     GP  N++V AILEKANAIRQ +
Sbjct: 1398 QVGPKIDERDSLLEQIRTKSFNLKPTAVTRHSIQGIQGPKTNLKVAAILEKANAIRQAL 1456

[Close]

BLAST of GT623421 vs. TrEMBL
Match: D7SR38_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_58.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030247001 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.234e-7
Identity = 29/58 (50.00%), Postives = 40/58 (68.97%), Query Frame = 3

Query:    3 KMGHETDEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            ++G + DER+  L QIR KSFNL+P    + +   GP  N++V A+LEKANAIRQ +A
Sbjct:   72 QIGPKVDERDSLLEQIRAKSFNLKPAAVPRPSIQ-GPRTNLKVAAMLEKANAIRQALA 128

[Close]

BLAST of GT623421 vs. SwissProt
Match: SCRL2_ORYSJ (SCAR-like protein 2 OS=Oryza sativa subsp. japonica GN=Os01g0208600 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.608e-11
Identity = 37/60 (61.67%), Postives = 45/60 (75.00%), Query Frame = 3

Query:    6 MGHET---DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            +G++T   DERE+ L QIR+K+FNLR T   K+NTS+  TAN  V AILEKANAIRQ VA
Sbjct: 1262 VGNDTKNLDEREELLQQIRSKTFNLRRTNASKTNTSSPTTANSSVVAILEKANAIRQAVA 1321

[Close]

BLAST of GT623421 vs. SwissProt
Match: SCAR3_ARATH (Protein SCAR3 OS=Arabidopsis thaliana GN=SCAR3 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.066e-8
Identity = 30/54 (55.56%), Postives = 37/54 (68.52%), Query Frame = 3

Query:   24 EREDFLYQIRTKSFNLRP---TVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            E  DFL QIRT+ FNLRP   T T  +  +T P  N +++AILEKAN+IRQ VA
Sbjct:  954 ETGDFLQQIRTQQFNLRPVVMTTTSSATATTDPIINTKISAILEKANSIRQAVA 1007

[Close]

BLAST of GT623421 vs. SwissProt
Match: SCAR2_ARATH (Protein SCAR2 OS=Arabidopsis thaliana GN=SCAR2 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.852e-8
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 3

Query:   21 DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            D+++  L QIR KS NL+P VT + +  TGP  +++V AILEKAN IR  +A
Sbjct: 1334 DDKDSLLAQIRNKSVNLKPAVTTRPSIQTGPRTDLRVAAILEKANTIRMAMA 1385

[Close]

BLAST of GT623421 vs. SwissProt
Match: SCAR1_ARATH (Protein SCAR1 OS=Arabidopsis thaliana GN=SCAR1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.984e-7
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 3

Query:    3 KMGH-ETDERE--DFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            K  H  T+E E  DFL+QIRTK FNLR  V  K+++S   T N  ++ ILEKAN+IRQ VA
Sbjct:  746 KQSHGNTNETEAGDFLHQIRTKQFNLRRVVRTKTSSSE-TTMNTNISVILEKANSIRQAVA 805

[Close]

BLAST of GT623421 vs. TAIR peptide
Match: AT1G29170.1 (| Symbols: WAVE2, ATSCAR3, SCAR3 | SCAR family protein | chr1:10190352-10194900 REVERSE LENGTH=1020)

HSP 1 Score: 57.7658 bits (138), Expect = 1.707e-9
Identity = 30/54 (55.56%), Postives = 37/54 (68.52%), Query Frame = 3

Query:   24 EREDFLYQIRTKSFNLRP---TVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            E  DFL QIRT+ FNLRP   T T  +  +T P  N +++AILEKAN+IRQ VA
Sbjct:  954 ETGDFLQQIRTQQFNLRPVVMTTTSSATATTDPIINTKISAILEKANSIRQAVA 1007

[Close]

BLAST of GT623421 vs. TAIR peptide
Match: AT2G38440.1 (| Symbols: ITB1, SCAR2, DIS3, WAVE4, ATSCAR2 | SCAR homolog 2 | chr2:16095550-16100851 FORWARD LENGTH=1399)

HSP 1 Score: 55.8398 bits (133), Expect = 6.485e-9
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 3

Query:   21 DEREDFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            D+++  L QIR KS NL+P VT + +  TGP  +++V AILEKAN IR  +A
Sbjct: 1334 DDKDSLLAQIRNKSVNLKPAVTTRPSIQTGPRTDLRVAAILEKANTIRMAMA 1385

[Close]

BLAST of GT623421 vs. TAIR peptide
Match: AT2G34150.2 (| Symbols: WAVE1 | SCAR family protein | chr2:14419432-14423259 REVERSE LENGTH=821)

HSP 1 Score: 53.9138 bits (128), Expect = 2.464e-8
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 3

Query:    3 KMGH-ETDERE--DFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            K  H  T+E E  DFL+QIRTK FNLR  V  K+++S   T N  ++ ILEKAN+IRQ VA
Sbjct:  746 KQSHGNTNETEAGDFLHQIRTKQFNLRRVVRTKTSSSE-TTMNTNISVILEKANSIRQAVA 805

[Close]

BLAST of GT623421 vs. TAIR peptide
Match: AT2G34150.1 (| Symbols: ATRANGAP2, ATSCAR1, SCAR1, WAVE1 | SCAR family protein | chr2:14419432-14422361 REVERSE LENGTH=700)

HSP 1 Score: 53.9138 bits (128), Expect = 2.464e-8
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 3

Query:    3 KMGH-ETDERE--DFLYQIRTKSFNLRPTVTGKSNTSTGPTANVQVTAILEKANAIRQVVA 176
            K  H  T+E E  DFL+QIRTK FNLR  V  K+++S   T N  ++ ILEKAN+IRQ VA
Sbjct:  625 KQSHGNTNETEAGDFLHQIRTKQFNLRRVVRTKTSSSE-TTMNTNISVILEKANSIRQAVA 684

[Close]

BLAST of GT623421 vs. TAIR peptide
Match: AT5G01730.1 (| Symbols: WAVE3, ATSCAR4, SCAR4 | SCAR family protein 4 | chr5:273019-277561 REVERSE LENGTH=1170)

HSP 1 Score: 50.447 bits (119), Expect = 2.724e-7
Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = 3

Query:    6 MGHETDEREDFLYQIRTKSFNLRPT-VTGKSNTSTG-PTANVQVTAILEKANAIRQVVA 176
            +G   DE +  L  IR+KSFNLRP   +G+ N     P  N++V AILEKAN +RQ +A
Sbjct: 1099 VGARVDENDSLLEIIRSKSFNLRPADASGRPNFQVAVPKTNLKVAAILEKANTLRQAMA 1157

The following BLAST results are available for this feature:

BLAST of GT623421 vs. TrEMBL
BLAST of GT623421 vs. SwissProt
BLAST of GT623421 vs. TAIR peptide

BLAST of GT623421 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9H2I1_POPTR	7.456e-12	66.07	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I1L8_POPTR	3.700e-11	66.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TJL3_VITVI	2.398e-10	65.45	Whole genome shotgun sequence of line PN40024, sca...	[more]
C7IWQ9_ORYSJ	5.343e-10	61.67	Os01g0208600 protein OS=Oryza sativa subsp. japoni...	[more]
B8ACP1_ORYSI	5.343e-10	61.67	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2ZQJ1_ORYSJ	5.343e-10	61.67	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q7XXS7_ORYSJ	1.190e-9	61.67	Putative uncharacterized protein (Fragment) OS=Ory...	[more]
B9S9B2_RICCO	2.030e-9	66.67	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9SL38_RICCO	8.531e-8	52.54	Protein SCAR2, putative OS=Ricinus communis GN=RCO...	[more]
D7SR38_VITVI	4.234e-7	50.00	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of GT623421 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
SCRL2_ORYSJ	2.608e-11	61.67	SCAR-like protein 2 OS=Oryza sativa subsp. japonic...	[more]
SCAR3_ARATH	2.066e-8	55.56	Protein SCAR3 OS=Arabidopsis thaliana GN=SCAR3 PE=...	[more]
SCAR2_ARATH	7.852e-8	50.00	Protein SCAR2 OS=Arabidopsis thaliana GN=SCAR2 PE=...	[more]
SCAR1_ARATH	2.984e-7	54.10	Protein SCAR1 OS=Arabidopsis thaliana GN=SCAR1 PE=...	[more]

BLAST of GT623421 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G29170.1	1.707e-9	55.56	\| Symbols: WAVE2, ATSCAR3, SCAR3 \| SCAR family pro...	[more]
AT2G38440.1	6.485e-9	50.00	\| Symbols: ITB1, SCAR2, DIS3, WAVE4, ATSCAR2 \| SCA...	[more]
AT2G34150.2	2.464e-8	54.10	\| Symbols: WAVE1 \| SCAR family protein \| chr2:1441...	[more]
AT2G34150.1	2.464e-8	54.10	\| Symbols: ATRANGAP2, ATSCAR1, SCAR1, WAVE1 \| SCAR...	[more]
AT5G01730.1	2.724e-7	47.46	\| Symbols: WAVE3, ATSCAR4, SCAR4 \| SCAR family pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 44..60 score
None	No IPR available	SEG	seg	seg	coord: 59..72 score
None	No IPR available	SEG	seg	seg	coord: 6..17 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623421 ID=GT623421; Name=GT623421; organism=Lens culinaris; type=EST; length=362bp
TGAAAATGGGACATGAGACAGATGAAAGAGAAGATTTCCTTTATCAAATC
AGAACCAAATCCTTCAACCTGAGGCCTACAGTAACAGGAAAGTCGAACAC
TTCAACGGGTCCCACTGCAAATGTCCAAGTTACAGCAATTTTGGAGAAAG
CAAATGCAATCCGTCAGGTTGTCGCAAGTGATGAAGGCGAAGACGACGAT
GATACCTGGAGTGATACATGATTTCTTTTTCTTTTTTGAAATTCTTTTAA
TTTTCATACATGAGTAGAGTCATCTGTTTCTTTTAGCCTCTTGAAGTGAA
GTTGCATTGCATGAGGCTTTGTTGTATGTACGCATGTGTGAATAAATCGT
GTTTATTTTTGT

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