GT624982

EST Overview


Name	GT624982
Unique Name	GT624982
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	492

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT624982 vs. TrEMBL
Match: D7UB90_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_24.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019326001 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.253e-35
Identity = 72/94 (76.60%), Postives = 87/94 (92.55%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADP+AETAEE LIKQ+M++DLEKVL+SLN REK+VIRWR+G++DGR KTLQEIGE+M VSRER+RQIE+CAFRKLKNK+R   LQQY+
Sbjct:  480 KPSEVIADPDAETAEELLIKQFMREDLEKVLNSLNPREKQVIRWRYGLEDGRTKTLQEIGELMGVSRERIRQIESCAFRKLKNKRRTKHLQQYL 573

[Close]

BLAST of GT624982 vs. TrEMBL
Match: Q710C3_SPIOL (Sigma factor OS=Spinacia oleracea GN=sig2 PE=2 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 2.137e-35
Identity = 72/96 (75.00%), Postives = 88/96 (91.67%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYMYL 290
            KPSEV+ADPEAETAE+ L KQ+M++DLEKVLD+L+ REK+VIRWRFG++DGRMKTLQEIGE+M VSRER+RQIE+ AFRKL+NKK+   LQQYM+L
Sbjct:  480 KPSEVIADPEAETAEDLLTKQFMRQDLEKVLDTLSSREKQVIRWRFGLEDGRMKTLQEIGELMGVSRERIRQIESSAFRKLQNKKKTINLQQYMFL 575

[Close]

BLAST of GT624982 vs. TrEMBL
Match: B9SDA3_RICCO (RNA polymerase sigma factor rpoD1, putative OS=Ricinus communis GN=RCOM_1515490 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.761e-35
Identity = 72/94 (76.60%), Postives = 87/94 (92.55%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEAETAEE L+KQ+MK+DLEKVL++LN RE +V+RWRFG++DGRMKTLQEIGE+M VSRER+RQIE+ AFRKLKNKKR  QL+QY+
Sbjct:  474 KPSEVIADPEAETAEELLMKQFMKQDLEKVLNTLNPRETQVVRWRFGLEDGRMKTLQEIGEIMGVSRERIRQIESSAFRKLKNKKRTKQLKQYL 567

[Close]

BLAST of GT624982 vs. TrEMBL
Match: D7KIT0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_470915 PE=4 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.061e-34
Identity = 73/94 (77.66%), Postives = 86/94 (91.49%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEA T+E+ LIK++M++DL+KVLDSL  REK+VIRWRFGM+DGRMKTLQEIGEMM VSRERVRQIE+ AFRKLKNKKR+  LQQY+
Sbjct:  475 KPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKKRSNHLQQYL 568

[Close]

BLAST of GT624982 vs. TrEMBL
Match: Q9MTH3_SINAL (Sigma factor 2 OS=Sinapis alba GN=sig2 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.809e-34
Identity = 72/94 (76.60%), Postives = 86/94 (91.49%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEAET+E+ L+KQ+M++DL+KVLDSL+ REK+VIRWRFGM+DGRMKTLQEIGE M VSRERVRQI+  AFRKLKNKKR + LQQY+
Sbjct:  475 KPSEVIADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGMEDGRMKTLQEIGETMGVSRERVRQIDTSAFRKLKNKKRNSHLQQYL 568

[Close]

BLAST of GT624982 vs. TrEMBL
Match: O22455_ARATH (Sigma factor 2 OS=Arabidopsis thaliana GN=SIG2 PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.363e-34
Identity = 73/94 (77.66%), Postives = 85/94 (90.43%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEA T+E+ LIK++M++DL+KVLDSL  REK+VIRWRFGM+DGRMKTLQEIGEMM VSRERVRQIE+ AFRKLKNKKR   LQQY+
Sbjct:  477 KPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKKRNNHLQQYL 570

[Close]

BLAST of GT624982 vs. TrEMBL
Match: O22056_ARATH (At1g08540 OS=Arabidopsis thaliana GN=SIG1 PE=2 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 2.363e-34
Identity = 73/94 (77.66%), Postives = 85/94 (90.43%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEA T+E+ LIK++M++DL+KVLDSL  REK+VIRWRFGM+DGRMKTLQEIGEMM VSRERVRQIE+ AFRKLKNKKR   LQQY+
Sbjct:  475 KPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKKRNNHLQQYL 568

[Close]

BLAST of GT624982 vs. TrEMBL
Match: Q8GWC1_ARATH (Putative plastid RNA polymerase sigma-subunit SIG1 OS=Arabidopsis thaliana GN=At1g08540/F22O13_2 PE=2 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.030e-34
Identity = 73/94 (77.66%), Postives = 84/94 (89.36%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEA T E+ LIK++M++DL+KVLDSL  REK+VIRWRFGM+DGRMKTLQEIGEMM VSRERVRQIE+ AFRKLKNKKR   LQQY+
Sbjct:  475 KPSEVIADPEAVTPEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKKRNNHLQQYL 568

[Close]

BLAST of GT624982 vs. TrEMBL
Match: B9IPZ1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_780378 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 7.601e-33
Identity = 70/94 (74.47%), Postives = 83/94 (88.30%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            K SEV ADPEAETAE+ L+K++MKKDLE+VL+SL+ RE +VIRWRFGM+DGRMKTLQEIGE+M VSRER+RQIE  AFRKLKNK R  QL+QY+
Sbjct:  469 KLSEVTADPEAETAEDLLMKEFMKKDLERVLESLSPRENQVIRWRFGMEDGRMKTLQEIGELMGVSRERIRQIELSAFRKLKNKNRTKQLRQYL 562

[Close]

BLAST of GT624982 vs. TrEMBL
Match: B9I6F1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834586 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.693e-32
Identity = 71/94 (75.53%), Postives = 81/94 (86.17%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            K SEV ADPEAETAE+ L+K++MKKDL KVL SL+ RE +V+RWRFGM+DGRMKTLQEIGE+M VSRERVRQIE  AFRKLKNK R  QL+QYM
Sbjct:  472 KLSEVTADPEAETAEDLLMKEFMKKDLAKVLGSLSPRENQVVRWRFGMEDGRMKTLQEIGELMGVSRERVRQIELSAFRKLKNKNRTKQLRQYM 565

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_ANASP (RNA polymerase sigma factor rpoD OS=Anabaena sp. (strain PCC 7120) GN=rpoD PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 7.279e-21
Identity = 46/87 (52.87%), Postives = 70/87 (80.46%), Query Frame = 3

Query:   24 DPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + ET E+ + K  +++DLEKVLDSL+ RE+ V+R R+G+DDGRMKTL+EIG++ +V+RER+RQIEA A RKL++  R + L++Y+
Sbjct:  303 ESDGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYI 389

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_SYNY3 (RNA polymerase sigma factor rpoD OS=Synechocystis sp. (strain PCC 6803) GN=rpoD PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 8.047e-20
Identity = 44/87 (50.57%), Postives = 69/87 (79.31%), Query Frame = 3

Query:   24 DPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + ET E+ + K  +++DLE VLD+L+ RE+ V+R R+G+DDGRMKTL+EIG++ +V+RER+RQIEA A RKL++  R + L++Y+
Sbjct:  338 EADGETPEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSILKEYI 424

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_MICAE (RNA polymerase sigma factor rpoD1 OS=Microcystis aeruginosa GN=rpoD1 PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 8.047e-20
Identity = 44/87 (50.57%), Postives = 69/87 (79.31%), Query Frame = 3

Query:   24 DPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + ET E+ + K  +++DLE VLD+L+ RE+ V+R R+G+DDGRMKTL+EIG++ +V+RER+RQIEA A RKL++  R + L++Y+
Sbjct:  329 EADGETPEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSILKEYI 415

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD1_SYNE7 (RNA polymerase sigma factor rpoD1 OS=Synechococcus elongatus (strain PCC 7942) GN=rpoD1 PE=3 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 3.994e-19
Identity = 43/87 (49.43%), Postives = 68/87 (78.16%), Query Frame = 3

Query:   24 DPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + ET E+ + K  +++DLE VL +L+ RE+ V+R R+G+DDGRMKTL+EIG++ +V+RER+RQIEA A RKL++  R + L++Y+
Sbjct:  312 EADGETPEDEVAKNLLREDLEGVLSTLSPRERDVLRLRYGLDDGRMKTLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSILKEYI 398

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAES (RNA polymerase sigma factor rpoD OS=Staphylococcus epidermidis (strain ATCC 12228) GN=rpoD PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAEQ (RNA polymerase sigma factor rpoD OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=rpoD PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAAW (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain MW2) GN=rpoD PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAAS (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain MSSA476) GN=rpoD PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAAR (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain MRSA252) GN=rpoD PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. SwissProt
Match: RPOD_STAAN (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain N315) GN=rpoD PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.813e-19
Identity = 42/91 (46.15%), Postives = 67/91 (73.63%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            + + D EA++  +H   + +K+ LE VLD+L  RE+ V+R RFG+DDGR +TL+E+G++  V+RER+RQIEA A RKL++  R+ +L+ +M
Sbjct:  277 DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFM 367

[Close]

BLAST of GT624982 vs. TAIR peptide
Match: AT1G08540.1 (| Symbols: SIGB, SIG1, SIG2, SIGA, ATSIG1, ABC1, ATSIG2 | RNApolymerase sigma subunit 2 | chr1:2703461-2706696 FORWARD LENGTH=572)

HSP 1 Score: 148.288 bits (373), Expect = 1.999e-36
Identity = 73/94 (77.66%), Postives = 85/94 (90.43%), Query Frame = 3

Query:    3 KPSEVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            KPSEV+ADPEA T+E+ LIK++M++DL+KVLDSL  REK+VIRWRFGM+DGRMKTLQEIGEMM VSRERVRQIE+ AFRKLKNKKR   LQQY+
Sbjct:  475 KPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKKRNNHLQQYL 568

[Close]

BLAST of GT624982 vs. TAIR peptide
Match: AT3G53920.1 (| Symbols: SIGC, SIG3 | RNApolymerase sigma-subunit C | chr3:19961041-19963820 REVERSE LENGTH=571)

HSP 1 Score: 70.4774 bits (171), Expect = 5.296e-13
Identity = 34/91 (37.36%), Postives = 60/91 (65.93%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            E   D   E+ EE +++Q  ++D+  +L+ L  REK+V+  R+G+ D R K+L+EIG+++ VS+E +R+IE  A  KL+++  A  L+ Y+
Sbjct:  479 EFTPDTTMESPEEAVMRQSARRDIHDLLEGLEPREKQVMVLRYGLQDYRPKSLEEIGKLLKVSKEWIRKIERRAMAKLRDQPNAEDLRYYL 569

[Close]

BLAST of GT624982 vs. TAIR peptide
Match: AT2G36990.1 (| Symbols: SIGF, SIG6, ATSIG6, SOLDAT8 | RNApolymerase sigma-subunit F | chr2:15537502-15540016 REVERSE LENGTH=547)

HSP 1 Score: 68.9366 bits (167), Expect = 1.541e-12
Identity = 34/90 (37.78%), Postives = 56/90 (62.22%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQY 281
            E+  D   ET    + KQ M+  +  +L+ L+ +E+R+I+ RFG+D G+ ++L EIGE+  +S+ERVRQ+E+ A  +LK    +  L  Y
Sbjct:  453 EITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQRSLSEIGEIYGLSKERVRQLESRALYRLKQNMNSHGLHAY 542

[Close]

BLAST of GT624982 vs. TAIR peptide
Match: AT5G13730.1 (| Symbols: SIG4, SIGD | sigma factor 4 | chr5:4429132-4430744 REVERSE LENGTH=419)

HSP 1 Score: 66.2402 bits (160), Expect = 9.988e-12
Identity = 33/90 (36.67%), Postives = 55/90 (61.11%), Query Frame = 3

Query:   12 EVMADPEAETAEEHLIKQYMKKDLEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQY 281
            E++  P+    EE + +++MK ++E++L SL  RE RV+   FG++     + +EIG+ + +SRERVRQI   A +KL+N      L+ Y
Sbjct:  325 EIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGLNGETPMSFEEIGKSLKLSRERVRQINGIALKKLRNVHNVNDLKIY 414

[Close]

BLAST of GT624982 vs. TAIR peptide
Match: AT5G24120.1 (| Symbols: SIGE, SIG5, ATSIG5 | sigma factor E | chr5:8157794-8159746 REVERSE LENGTH=517)

HSP 1 Score: 56.9954 bits (136), Expect = 6.060e-9
Identity = 31/68 (45.59%), Postives = 44/68 (64.71%), Query Frame = 3

Query:   81 LEKVLDSLNQREKRVIRWRFGMDDGRMKTLQEIGEMMDVSRERVRQIEACAFRKLKNKKRAAQLQQYM 284
            L+ VLDSL  +E  VIR R+G+D    +TL EI   +++SRE VR+ E  A  KLK++ R   L+QY+
Sbjct:  449 LDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDYLRQYI 516

The following BLAST results are available for this feature:

BLAST of GT624982 vs. TrEMBL
BLAST of GT624982 vs. SwissProt
BLAST of GT624982 vs. TAIR peptide

BLAST of GT624982 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7UB90_VITVI	1.253e-35	76.60	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q710C3_SPIOL	2.137e-35	75.00	Sigma factor OS=Spinacia oleracea GN=sig2 PE=2 SV=...	[more]
B9SDA3_RICCO	4.761e-35	76.60	RNA polymerase sigma factor rpoD1, putative OS=Ric...	[more]
D7KIT0_ARALY	1.061e-34	77.66	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9MTH3_SINAL	1.809e-34	76.60	Sigma factor 2 OS=Sinapis alba GN=sig2 PE=2 SV=1	[more]
O22455_ARATH	2.363e-34	77.66	Sigma factor 2 OS=Arabidopsis thaliana GN=SIG2 PE=...	[more]
O22056_ARATH	2.363e-34	77.66	At1g08540 OS=Arabidopsis thaliana GN=SIG1 PE=2 SV=...	[more]
Q8GWC1_ARATH	4.030e-34	77.66	Putative plastid RNA polymerase sigma-subunit SIG1...	[more]
B9IPZ1_POPTR	7.601e-33	74.47	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I6F1_POPTR	1.693e-32	75.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GT624982 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPOD_ANASP	7.279e-21	52.87	RNA polymerase sigma factor rpoD OS=Anabaena sp. (...	[more]
RPOD_SYNY3	8.047e-20	50.57	RNA polymerase sigma factor rpoD OS=Synechocystis ...	[more]
RPOD_MICAE	8.047e-20	50.57	RNA polymerase sigma factor rpoD1 OS=Microcystis a...	[more]
RPOD1_SYNE7	3.994e-19	49.43	RNA polymerase sigma factor rpoD1 OS=Synechococcus...	[more]
RPOD_STAES	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAEQ	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAW	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAS	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAR	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAN	6.813e-19	46.15	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]

BLAST of GT624982 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G08540.1	1.999e-36	77.66	\| Symbols: SIGB, SIG1, SIG2, SIGA, ATSIG1, ABC1, A...	[more]
AT3G53920.1	5.296e-13	37.36	\| Symbols: SIGC, SIG3 \| RNApolymerase sigma-subuni...	[more]
AT2G36990.1	1.541e-12	37.78	\| Symbols: SIGF, SIG6, ATSIG6, SOLDAT8 \| RNApolyme...	[more]
AT5G13730.1	9.988e-12	36.67	\| Symbols: SIG4, SIGD \| sigma factor 4 \| chr5:4429...	[more]
AT5G24120.1	6.060e-9	45.59	\| Symbols: SIGE, SIG5, ATSIG5 \| sigma factor E \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000943	RNA polymerase sigma-70 factor	PRINTS	PR00046	SIGMA70FCT	coord: 34..46 score: 2.1e-14coord: 55..70 score: 2.1e-14coord: 70..81 score: 2.1
IPR000943	RNA polymerase sigma-70 factor	PROSITE	PS00716	SIGMA70_2	coord: 55..81 score
IPR007630	RNA polymerase sigma-70 region 4	PFAM	PF04545	Sigma70_r4	coord: 30..83 score: 2
IPR011991	Winged helix-turn-helix transcription repressor DNA-binding	GENE3D	1.10.10.10	no description	coord: 25..94 score: 1.5
IPR013324	RNA polymerase sigma factor, region 3/4	SUPERFAMILY	88659	Sigma3 and sigma4 domains of RNA polymerase sigma factors	coord: 16..94 score: 1.5
None	No IPR available	SEG	seg	seg	coord: 16..29 score: NAcoord: 30..48 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT624982 ID=GT624982; Name=GT624982; organism=Lens culinaris; type=EST; length=492bp
TTAAACCTTCAGAAGTAATGGCTGATCCGGAAGCTGAAACAGCGGAAGAA
CATCTCATTAAGCAGTACATGAAAAAGGACCTAGAAAAGGTACTAGACAG
CCTGAATCAGAGAGAGAAACGTGTGATTCGATGGAGATTTGGTATGGACG
ATGGAAGGATGAAGACACTGCAAGAGATTGGGGAAATGATGGACGTGAGT
AGGGAGAGAGTTAGACAAATTGAGGCATGTGCTTTTAGGAAGCTTAAGAA
CAAGAAGAGAGCCGCACAATTGCAGCAGTATATGTATTTATAACCCAGCC
AAATATTGATTTAGCTTATTTCAGGTCCTGATATCTTTGTTCTTTACATG
TATGTTGTTCCTCTACTTTTTCTTCATTATTTAAGAGAAAACAAAAAGGT
TATTTCAGTATACTAGGGATCAGAAAATCAATGTAAACACGAGCAAATAA
CTTGACCAAGAGTTCATCATTTTTTAATGTATTAAAATTTTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	DNA-binding transcription factor activity
GO:0016987	sigma factor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006352	DNA-templated transcription initiation

Vocabulary: **INTERPRO**
Term	Definition
IPR000943	RNA_pol_sigma70
IPR007630	RNA_pol_sigma70_r4
IPR011991	ArsR-like_HTH
IPR013324	RNA_pol_sigma_r3/r4-like

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