GT625826

EST Overview


Name	GT625826
Unique Name	GT625826
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	485

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT625826 vs. TrEMBL
Match: B9RQ07_RICCO (Peroxisomal abc transporter, putative OS=Ricinus communis GN=RCOM_1662980 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.963e-22
Identity = 51/59 (86.44%), Postives = 54/59 (91.53%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIK 178
            LDECTNATSVDVEE LY LAK MDITV+TSSQRPALIPFHS+ELR IDGEGNW+LR IK
Sbjct: 1280 LDECTNATSVDVEEQLYRLAKDMDITVVTSSQRPALIPFHSVELRFIDGEGNWELRTIK 1338

[Close]

BLAST of GT625826 vs. TrEMBL
Match: C5YY24_SORBI (Putative uncharacterized protein Sb09g000670 OS=Sorghum bicolor GN=Sb09g000670 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.502e-20
Identity = 49/60 (81.67%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFH++EL+LIDGEGNW+L  I+Q
Sbjct: 1205 LDECTNATSVDVEEHLYRLATSMGITVITSSQRPALIPFHALELKLIDGEGNWELCAIQQ 1264

[Close]

BLAST of GT625826 vs. TrEMBL
Match: C0PJC8_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.502e-20
Identity = 49/60 (81.67%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFH++EL+LIDGEGNW+L  I+Q
Sbjct:  103 LDECTNATSVDVEEHLYRLATSMGITVITSSQRPALIPFHALELKLIDGEGNWELCAIQQ 162

[Close]

BLAST of GT625826 vs. TrEMBL
Match: B9H532_POPTR (Peroxisomal membrane ABC transporter family, PMP family OS=Populus trichocarpa GN=POPTRDRAFT_831368 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.502e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY LA  M IT ITSSQRPALIPFHS+ELRLIDGEG+W+LR IKQ
Sbjct: 1250 LDECTNATSVDVEEQLYRLASDMGITFITSSQRPALIPFHSLELRLIDGEGHWELRAIKQ 1309

[Close]

BLAST of GT625826 vs. TrEMBL
Match: Q8GU57_ORYSJ (Peroxisomal membrane protein ABC transporter homologue OS=Oryza sativa subsp. japonica GN=pmp2 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.961e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFHS+EL+LIDGEGNW+L  I Q
Sbjct: 1185 LDECTNATSVDVEEHLYKLATSMGITVITSSQRPALIPFHSLELKLIDGEGNWELCEIHQ 1244

[Close]

BLAST of GT625826 vs. TrEMBL
Match: Q65X28_ORYSJ (Putative ABC transporter OS=Oryza sativa subsp. japonica GN=OSJNBa0068N01.7 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.961e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFHS+EL+LIDGEGNW+L  I Q
Sbjct:  709 LDECTNATSVDVEEHLYKLATSMGITVITSSQRPALIPFHSLELKLIDGEGNWELCEIHQ 768

[Close]

BLAST of GT625826 vs. TrEMBL
Match: Q0DLD4_ORYSJ (Os05g0107600 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os05g0107600 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.961e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFHS+EL+LIDGEGNW+L  I Q
Sbjct: 1091 LDECTNATSVDVEEHLYKLATSMGITVITSSQRPALIPFHSLELKLIDGEGNWELCEIHQ 1150

[Close]

BLAST of GT625826 vs. TrEMBL
Match: B9FM21_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_16830 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.961e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFHS+EL+LIDGEGNW+L  I Q
Sbjct: 1262 LDECTNATSVDVEEHLYKLATSMGITVITSSQRPALIPFHSLELKLIDGEGNWELCEIHQ 1321

[Close]

BLAST of GT625826 vs. TrEMBL
Match: B8AWX1_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18139 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.961e-20
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEEHLY LA  M ITVITSSQRPALIPFHS+EL+LIDGEGNW+L  I Q
Sbjct: 1262 LDECTNATSVDVEEHLYKLATSMGITVITSSQRPALIPFHSLELKLIDGEGNWELCEIHQ 1321

[Close]

BLAST of GT625826 vs. TrEMBL
Match: D7M963_ARALY (Peroxisomal abc transporter OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_490770 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.562e-20
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY +A+ M +T ITSSQRPALIPFHS+ELRLIDGEGNW+LR I+Q
Sbjct: 1275 LDECTNATSVDVEEQLYRVARDMGVTFITSSQRPALIPFHSLELRLIDGEGNWELRSIEQ 1334

[Close]

BLAST of GT625826 vs. SwissProt
Match: AB1D_ARATH (ABC transporter D family member 1 OS=Arabidopsis thaliana GN=ABCC1 PE=1 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.846e-21
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY +A+ M +T ITSSQRPALIPFHS+ELRLIDGEGNW+LR I+Q
Sbjct: 1275 LDECTNATSVDVEEQLYRVARDMGVTFITSSQRPALIPFHSLELRLIDGEGNWELRSIEQ 1334

HSP 2 Score: 53.9138 bits (128), Expect = 4.414e-7
Identity = 26/55 (47.27%), Postives = 34/55 (61.82%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQL 166
            LDECT+A + D+EE      + M  + IT S RPAL+ FH + L L DGEG W +
Sbjct:  605 LDECTSAVTTDMEERFAAKVRAMGTSCITISHRPALVAFHDVVLSL-DGEGGWSV 658

[Close]

BLAST of GT625826 vs. SwissProt
Match: PXA1_YEAST (Peroxisomal long-chain fatty acid import protein 2 OS=Saccharomyces cerevisiae GN=PXA1 PE=1 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 1.161e-7
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDG-EGNWQLRLI 175
            LDE TNA SVD+E++L+ L K+     I+ SQRP LI +H M L + +  +G WQL+ +
Sbjct:  772 LDEATNAISVDMEDYLFNLLKRYRFNFISISQRPTLIKYHEMLLEIGENRDGKWQLQAV 830

[Close]

BLAST of GT625826 vs. SwissProt
Match: ABCD3_RAT (ATP-binding cassette sub-family D member 3 OS=Rattus norvegicus GN=Abcd3 PE=1 SV=3)

HSP 1 Score: 53.5286 bits (127), Expect = 5.764e-7
Identity = 26/60 (43.33%), Postives = 39/60 (65.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECT+A SVDVE+++Y   +K+ IT+ T S R +L   H   L + DG GN++ + I +
Sbjct:  594 LDECTSAVSVDVEDYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHM-DGRGNYEFKKITE 652

[Close]

BLAST of GT625826 vs. TAIR peptide
Match: AT4G39850.2 (| Symbols: PXA1 | peroxisomal ABC transporter 1 | chr4:18489220-18496762 FORWARD LENGTH=1338)

HSP 1 Score: 101.679 bits (252), Expect = 2.121e-22
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY +A+ M +T ITSSQRPALIPFHS+ELRLIDGEGNW+LR I+Q
Sbjct: 1276 LDECTNATSVDVEEQLYRVARDMGVTFITSSQRPALIPFHSLELRLIDGEGNWELRSIEQ 1335

HSP 2 Score: 53.9138 bits (128), Expect = 5.072e-8
Identity = 26/55 (47.27%), Postives = 34/55 (61.82%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQL 166
            LDECT+A + D+EE      + M  + IT S RPAL+ FH + L L DGEG W +
Sbjct:  605 LDECTSAVTTDMEERFAAKVRAMGTSCITISHRPALVAFHDVVLSL-DGEGGWSV 658

[Close]

BLAST of GT625826 vs. TAIR peptide
Match: AT4G39850.3 (| Symbols: PXA1 | peroxisomal ABC transporter 1 | chr4:18489220-18496762 FORWARD LENGTH=1352)

HSP 1 Score: 101.679 bits (252), Expect = 2.121e-22
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY +A+ M +T ITSSQRPALIPFHS+ELRLIDGEGNW+LR I+Q
Sbjct: 1290 LDECTNATSVDVEEQLYRVARDMGVTFITSSQRPALIPFHSLELRLIDGEGNWELRSIEQ 1349

HSP 2 Score: 53.9138 bits (128), Expect = 5.072e-8
Identity = 26/55 (47.27%), Postives = 34/55 (61.82%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQL 166
            LDECT+A + D+EE      + M  + IT S RPAL+ FH + L L DGEG W +
Sbjct:  605 LDECTSAVTTDMEERFAAKVRAMGTSCITISHRPALVAFHDVVLSL-DGEGGWSV 658

[Close]

BLAST of GT625826 vs. TAIR peptide
Match: AT4G39850.1 (| Symbols: PXA1, PED3, CTS, ACN2, AtABCD1 | peroxisomal ABC transporter 1 | chr4:18489220-18496762 FORWARD LENGTH=1337)

HSP 1 Score: 101.679 bits (252), Expect = 2.121e-22
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQLRLIKQ 181
            LDECTNATSVDVEE LY +A+ M +T ITSSQRPALIPFHS+ELRLIDGEGNW+LR I+Q
Sbjct: 1275 LDECTNATSVDVEEQLYRVARDMGVTFITSSQRPALIPFHSLELRLIDGEGNWELRSIEQ 1334

HSP 2 Score: 53.9138 bits (128), Expect = 5.072e-8
Identity = 26/55 (47.27%), Postives = 34/55 (61.82%), Query Frame = 2

Query:    2 LDECTNATSVDVEEHLYGLAKKMDITVITSSQRPALIPFHSMELRLIDGEGNWQL 166
            LDECT+A + D+EE      + M  + IT S RPAL+ FH + L L DGEG W +
Sbjct:  605 LDECTSAVTTDMEERFAAKVRAMGTSCITISHRPALVAFHDVVLSL-DGEGGWSV 658

The following BLAST results are available for this feature:

BLAST of GT625826 vs. TrEMBL
BLAST of GT625826 vs. SwissProt
BLAST of GT625826 vs. TAIR peptide

BLAST of GT625826 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RQ07_RICCO	7.963e-22	86.44	Peroxisomal abc transporter, putative OS=Ricinus c...	[more]
C5YY24_SORBI	1.502e-20	81.67	Putative uncharacterized protein Sb09g000670 OS=So...	[more]
C0PJC8_MAIZE	1.502e-20	81.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B9H532_POPTR	1.502e-20	83.33	Peroxisomal membrane ABC transporter family, PMP f...	[more]
Q8GU57_ORYSJ	1.961e-20	83.33	Peroxisomal membrane protein ABC transporter homol...	[more]
Q65X28_ORYSJ	1.961e-20	83.33	Putative ABC transporter OS=Oryza sativa subsp. ja...	[more]
Q0DLD4_ORYSJ	1.961e-20	83.33	Os05g0107600 protein (Fragment) OS=Oryza sativa su...	[more]
B9FM21_ORYSJ	1.961e-20	83.33	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AWX1_ORYSI	1.961e-20	83.33	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7M963_ARALY	2.562e-20	80.00	Peroxisomal abc transporter OS=Arabidopsis lyrata ...	[more]

BLAST of GT625826 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
AB1D_ARATH	1.846e-21	80.00	ABC transporter D family member 1 OS=Arabidopsis t...	[more]
PXA1_YEAST	1.161e-7	45.76	Peroxisomal long-chain fatty acid import protein 2...	[more]
ABCD3_RAT	5.764e-7	43.33	ATP-binding cassette sub-family D member 3 OS=Ratt...	[more]

BLAST of GT625826 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G39850.2	2.121e-22	80.00	\| Symbols: PXA1 \| peroxisomal ABC transporter 1 \| ...	[more]
AT4G39850.3	2.121e-22	80.00	\| Symbols: PXA1 \| peroxisomal ABC transporter 1 \| ...	[more]
AT4G39850.1	2.121e-22	80.00	\| Symbols: PXA1, PED3, CTS, ACN2, AtABCD1 \| peroxi...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11384	ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER	coord: 1..58 score: 4

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT625826 ID=GT625826; Name=GT625826; organism=Lens culinaris; type=EST; length=485bp
CTTGGACGAGTGTACCAATGCAACAAGTGTTGACGTTGAAGAACACCTAT
ATGGGCTAGCAAAGAAAATGGATATCACTGTTATTACTTCCTCACAACGA
CCTGCTTTAATACCATTTCACTCCATGGAATTGCGGCTGATTGATGGCGA
GGGTAATTGGCAACTCCGTTTGATCAAGCAATGATTCTTCGGTAGGTAGT
GTTGATGCAGACTCTGATAGTTAGTATAAGGTTGGTCGATGATGGTATCC
CTATCTTCTTTCATTTTGGGTTTACCATATTACTATATTCTCCCAGCTTC
AAGTTTTGCAGCAACAACCAAAATGGTTCATACAAAGTTGAAACACAACT
GTCATTATTGCTGCAGTATATTAATATCAATCATGTACAGTCTCCCCCTC
ATTCTTAGGTAGTTAATATTTATTCATATTTTGTAACCATTGTTTTAATA
ATAAAAAAATAGCTTGAAATATAGTAGCTCTTTGA

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