GT627892

EST Overview
NameGT627892
Unique NameGT627892
TypeEST
OrganismLens culinaris (lentil)
Sequence length544
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Lens culinaris unigene v12010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Lens culinaris unigene v12011-01-06
Homology
[Close]
BLAST of GT627892 vs. TrEMBL
Match: C6SXD7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.188e-41
Identity = 68/85 (80.00%), Postives = 74/85 (87.06%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL+DPASKAYEQ  AP+HGWAIRKAVSAGMY LPTKEQLLKKLNEDEASAK  +Q Y +AS  LI+YIDKLFVSR+LG DW
Sbjct:  124 TEGNSLRDPASKAYEQVFAPHHGWAIRKAVSAGMYALPTKEQLLKKLNEDEASAKDHVQSYVTASAPLIQYIDKLFVSRDLGIDW 208

HSP 2 Score: 53.9138 bits (128), Expect = 3.188e-41
Identity = 27/35 (77.14%), Postives = 29/35 (82.86%), Query Frame = 3 
Query:    6 QTLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDM 110
            Q L SLI+ DVQAN+VR  GS TRNLLRVKRGLDM
Sbjct:   79 QNLQSLIELDVQANTVRKGGSHTRNLLRVKRGLDM 113
[Close]
BLAST of GT627892 vs. TrEMBL
Match: C6SW52_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.189e-41
Identity = 68/85 (80.00%), Postives = 74/85 (87.06%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL++PASKAYEQ  AP+HGWAIRKAVS GMYVLPTKEQLLKKLNEDEASAK  MQ Y +AS  LI+YIDKLFVSR+LG DW
Sbjct:  122 TEGNSLRNPASKAYEQVFAPHHGWAIRKAVSVGMYVLPTKEQLLKKLNEDEASAKGHMQSYVTASALLIRYIDKLFVSRDLGIDW 206

HSP 2 Score: 53.9138 bits (128), Expect = 3.189e-41
Identity = 27/35 (77.14%), Postives = 29/35 (82.86%), Query Frame = 3 
Query:    6 QTLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDM 110
            Q L SLI+ DVQAN+VR  GS TRNLLRVKRGLDM
Sbjct:   77 QNLQSLIELDVQANTVRKGGSHTRNLLRVKRGLDM 111
[Close]
BLAST of GT627892 vs. TrEMBL
Match: B9H9V3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831834 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.165e-37
Identity = 62/85 (72.94%), Postives = 70/85 (82.35%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSLK PASKAY Q  AP+HGWAIRKAV+AGMY LPTK QLLKKLNEDE+SA +QMQ Y +AS  +I Y+DKLF+SR LG DW
Sbjct:  121 TEGNSLKGPASKAYAQVFAPHHGWAIRKAVAAGMYALPTKAQLLKKLNEDESSAIIQMQSYVAASAPVIMYVDKLFLSRELGIDW 205

HSP 2 Score: 51.6026 bits (122), Expect = 1.165e-37
Identity = 24/34 (70.59%), Postives = 29/34 (85.29%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDM 110
            TL S++D+DV+ NSVR  GS +RNLLRVKRGLDM
Sbjct:   77 TLQSVLDKDVERNSVRKGGSHSRNLLRVKRGLDM 110
[Close]
BLAST of GT627892 vs. TrEMBL
Match: B9II75_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_748049 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.260e-36
Identity = 59/85 (69.41%), Postives = 69/85 (81.18%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSLK PASKAYEQ  AP+HGWAIRKAV+AGMY LPTK QLL  LNEDE+SA +QMQ Y +AS  +I Y++KLF++R LG DW
Sbjct:  121 TEGNSLKGPASKAYEQVFAPHHGWAIRKAVAAGMYALPTKAQLLNNLNEDESSASIQMQSYVAASAPVIMYVEKLFLTRGLGIDW 205

HSP 2 Score: 51.2174 bits (121), Expect = 1.260e-36
Identity = 25/36 (69.44%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            TL SL+++D+Q NSVR SGS +RNLLRVKRGL M K
Sbjct:   77 TLQSLLEKDIQGNSVRKSGSHSRNLLRVKRGLVMVK 112
[Close]
BLAST of GT627892 vs. TrEMBL
Match: B9T461_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0290920 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.137e-36
Identity = 61/85 (71.76%), Postives = 71/85 (83.53%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSLKDPASKAY Q  AP+HGWAIRKAV+AGMY LPTK QLL KLNEDE+SAK+QM+ Y +AS  +I +IDKLF+S+ LG DW
Sbjct:  128 TEGNSLKDPASKAYAQVFAPHHGWAIRKAVAAGMYALPTKSQLLNKLNEDESSAKIQMEYYIAASAPVILFIDKLFLSKKLGIDW 212

HSP 2 Score: 46.9802 bits (110), Expect = 2.137e-36
Identity = 20/36 (55.56%), Postives = 29/36 (80.56%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            T+  ++++D++ N VR +GS +RNLLRVKRGLDM K
Sbjct:   84 TIQEMVNKDIEENCVRKAGSHSRNLLRVKRGLDMVK 119
[Close]
BLAST of GT627892 vs. TrEMBL
Match: B4G0V8_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.310e-35
Identity = 60/85 (70.59%), Postives = 67/85 (78.82%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL+D AS AY Q  AP+HGWAIRKAVSAGMY LP+K QLLKKLNEDE SAKVQMQ +  +S  +I Y+D LF SRNLG DW
Sbjct:  122 TEGNSLRDAASVAYAQVFAPHHGWAIRKAVSAGMYALPSKSQLLKKLNEDEESAKVQMQNFVRSSAPVICYVDDLFTSRNLGIDW 206

HSP 2 Score: 50.447 bits (119), Expect = 2.310e-35
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            TL S+++RD+Q ++VR  GS TRNLLRVKRG+DM K
Sbjct:   78 TLPSMVERDIQTDTVRKPGSHTRNLLRVKRGIDMVK 113
[Close]
BLAST of GT627892 vs. TrEMBL
Match: B6T2C2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.322e-35
Identity = 60/85 (70.59%), Postives = 67/85 (78.82%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL+D AS AY Q  AP+HGWAIRKAVSAGMY LP+K QLLKKLNEDE SAKVQMQ +  +S  +I Y+D LF SRNLG DW
Sbjct:   91 TEGNSLRDAASVAYAQVFAPHHGWAIRKAVSAGMYALPSKSQLLKKLNEDEESAKVQMQNFVRSSAPVICYVDDLFTSRNLGIDW 175

HSP 2 Score: 50.447 bits (119), Expect = 2.322e-35
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            TL S+++RD+Q ++VR  GS TRNLLRVKRG+DM K
Sbjct:   47 TLPSMVERDIQTDTVRKPGSHTRNLLRVKRGIDMVK 82
[Close]
BLAST of GT627892 vs. TrEMBL
Match: D7TKH1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021615001 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.471e-34
Identity = 56/82 (68.29%), Postives = 70/82 (85.37%), Query Frame = 1 
Query:  115 NSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            NSL++PAS AY + LAP+HGWAIRKAV+AGMYVLPTK QLL +LNEDEA+A++QMQ Y +AS  +I YI+KLF++R LG DW
Sbjct:  124 NSLRNPASTAYAEVLAPHHGWAIRKAVAAGMYVLPTKAQLLARLNEDEATARIQMQNYINASAPVILYINKLFLTRELGIDW 205

HSP 2 Score: 50.447 bits (119), Expect = 1.471e-34
Identity = 22/34 (64.71%), Postives = 29/34 (85.29%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDM 110
            TL++L+D D++ N VR +GS +RNLLRVKRGLDM
Sbjct:   77 TLSALLDHDIEGNCVRKAGSNSRNLLRVKRGLDM 110
[Close]
BLAST of GT627892 vs. TrEMBL
Match: C5WY80_SORBI (Putative uncharacterized protein Sb01g006060 OS=Sorghum bicolor GN=Sb01g006060 PE=4 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 3.250e-34
Identity = 59/85 (69.41%), Postives = 67/85 (78.82%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL+D AS AY Q  AP+HGWAIRKAV+AGMY LP+K QLLKKLNEDE SAKVQMQ +  +S  +I Y+D LF SRNLG DW
Sbjct:  122 TEGNSLRDAASVAYAQVFAPHHGWAIRKAVAAGMYALPSKSQLLKKLNEDEESAKVQMQNFVRSSAPVICYVDDLFTSRNLGIDW 206

HSP 2 Score: 47.7506 bits (112), Expect = 3.250e-34
Identity = 22/36 (61.11%), Postives = 29/36 (80.56%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            TL S+++ D+Q ++VR  GS TRNLLRVKRG+DM K
Sbjct:   78 TLPSMVELDIQTDTVRKPGSHTRNLLRVKRGIDMVK 113
[Close]
BLAST of GT627892 vs. TrEMBL
Match: Q10CF2_ORYSJ (Expressed protein OS=Oryza sativa subsp. japonica GN=Os03g0784900 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.218e-33
Identity = 60/85 (70.59%), Postives = 68/85 (80.00%), Query Frame = 1 
Query:  106 TWGNSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            T GNSL+D ASKAY Q  AP+HGWAIRKAV+AGMY LP+K QLLKKLNEDE SAKVQMQ +  AS  +I Y++ LF SRNLG DW
Sbjct:  123 TEGNSLRDAASKAYAQVFAPHHGWAIRKAVAAGMYALPSKSQLLKKLNEDENSAKVQMQTFIRASGPVILYVEDLFTSRNLGMDW 207

HSP 2 Score: 43.5134 bits (101), Expect = 1.218e-33
Identity = 19/35 (54.29%), Postives = 27/35 (77.14%), Query Frame = 3 
Query:   12 LNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            L  +++ D+Q  +VR +GS TRN+LRVKRG+DM K
Sbjct:   80 LPEMVELDIQKGTVRQAGSHTRNMLRVKRGIDMVK 114
[Close]
BLAST of GT627892 vs. TAIR peptide
Match: AT2G34690.1 (| Symbols: ACD11 | Glycolipid transfer protein (GLTP) family protein | chr2:14630425-14631779 FORWARD LENGTH=206)

HSP 1 Score: 114.005 bits (284), Expect = 3.041e-34
Identity = 52/82 (63.41%), Postives = 63/82 (76.83%), Query Frame = 1 
Query:  115 NSLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            NSLKDPA+K+Y Q  AP+HGWAIRKAVS GMY LPT+  LL  L EDEA+AK+ MQ Y ++S  LI Y+D LF+S+ LG DW
Sbjct:  125 NSLKDPATKSYAQVFAPHHGWAIRKAVSLGMYALPTRAHLLNMLKEDEAAAKIHMQSYVNSSAPLITYLDNLFLSKQLGIDW 206

HSP 2 Score: 48.521 bits (114), Expect = 3.041e-34
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:    9 TLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDMGK 116
            TL  ++D+D++A+ VR +GS TRNLLRVKRGLDM K
Sbjct:   77 TLVVMMDKDIEADCVRKAGSHTRNLLRVKRGLDMVK 112
[Close]
BLAST of GT627892 vs. TAIR peptide
Match: AT4G39670.1 (| Symbols: | Glycolipid transfer protein (GLTP) family protein | chr4:18410172-18410861 FORWARD LENGTH=229)

HSP 1 Score: 95.5153 bits (236), Expect = 5.403e-26
Identity = 43/81 (53.09%), Postives = 58/81 (71.60%), Query Frame = 1 
Query:  118 SLKDPASKAYEQALAPYHGWAIRKAVSAGMYVLPTKEQLLKKLNEDEASAKVQMQIYASASEALIKYIDKLFVSRNLGTDW 360
            SLKD A+ AY +  AP+H WA+R AV AGMY LPT++QLL +LNE + S +  M+ Y  AS  +I+YIDKL++ RN+  DW
Sbjct:  149 SLKDAATTAYTEVCAPFHTWAVRTAVYAGMYTLPTRDQLLLRLNETDQSVEKNMRRYMEASRPIIEYIDKLYIERNIKLDW 229

HSP 2 Score: 39.2762 bits (90), Expect = 5.403e-26
Identity = 16/35 (45.71%), Postives = 28/35 (80.00%), Query Frame = 3 
Query:    6 QTLNSLIDRDVQANSVRNSGSGTRNLLRVKRGLDM 110
            +TL++++D DV+  +V+  GS +RNL RV++GLD+
Sbjct:  100 ETLHNILDLDVEKETVKTPGSHSRNLRRVRQGLDL 134
The following BLAST results are available for this feature:
BLAST of GT627892 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6SXD7_SOYBN3.188e-4180.00Putative uncharacterized protein OS=Glycine max PE... [more]
C6SW52_SOYBN3.189e-4180.00Putative uncharacterized protein OS=Glycine max PE... [more]
B9H9V3_POPTR1.165e-3772.94Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9II75_POPTR1.260e-3669.41Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9T461_RICCO2.137e-3671.76Putative uncharacterized protein OS=Ricinus commun... [more]
B4G0V8_MAIZE2.310e-3570.59Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
B6T2C2_MAIZE2.322e-3570.59Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
D7TKH1_VITVI1.471e-3468.29Whole genome shotgun sequence of line PN40024, sca... [more]
C5WY80_SORBI3.250e-3469.41Putative uncharacterized protein Sb01g006060 OS=So... [more]
Q10CF2_ORYSJ1.218e-3370.59Expressed protein OS=Oryza sativa subsp. japonica ... [more]
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BLAST of GT627892 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT2G34690.13.041e-3463.41| Symbols: ACD11 | Glycolipid transfer protein (GL... [more]
AT4G39670.15.403e-2653.09| Symbols: | Glycolipid transfer protein (GLTP) f... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014830Glycolipid transfer protein domainGENE3D1.10.3520.10no descriptioncoord: 8..88
score: 3.9
IPR014830Glycolipid transfer protein domainPFAMPF08718GLTPcoord: 9..54
score: 4.9
IPR014830Glycolipid transfer protein domainSUPERFAMILY110004Glycolipid transfer protein, GLTPcoord: 7..89
score: 4.2
NoneNo IPR availablePANTHERPTHR10219GLYCOLIPID TRANSFER PROTEIN-RELATEDcoord: 4..89
score: 1.5

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT627892 ID=GT627892; Name=GT627892; organism=Lens culinaris; type=EST; length=544bp
CCACTCAGACGTTAAATTCATTGATTGATCGAGATGTACAAGCCAATTCT
GTGAGGAATTCAGGCAGCGGTACTAGAAATCTTTTGAGAGTGAAACGCGG
GCTCGACATGGGGAAATTCCCTTAAGGATCCAGCTTCAAAAGCTTACGAA
CAAGCGCTTGCGCCTTATCATGGTTGGGCTATCAGGAAGGCTGTTTCCGC
TGGGATGTATGTTCTTCCTACAAAGGAACAGCTTTTGAAGAAACTCAACG
AGGATGAGGCATCTGCGAAAGTCCAAATGCAAATTTATGCATCAGCATCC
GAAGCTCTAATCAAATACATTGATAAACTCTTTGTTTCCAGAAACTTAGG
AACAGATTGGTGAACCTTGGACTGTATTCAGATTGTGTACAATATGATTT
TCTATTAGGAAGTTGTATGAGATTATTAATGTATTTATTAGTAATCATTT
CTTATAGAATAATTTATCTATATAGCAATATAGAATGAACGTTTAAGAAA
ACTTTTCTTGAGAAGGATAACATTACTTGATTTCATTTCTCTCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0017089glycolipid transfer activity
GO:0051861glycolipid binding
Vocabulary: Biological Process
TermDefinition
GO:0046836glycolipid transport
Vocabulary: INTERPRO
TermDefinition
IPR014830Glycolipid_transfer_prot_dom