GT620348

EST Overview


Name	GT620348
Unique Name	GT620348
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	743

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT620348 vs. TrEMBL
Match: B7FF76_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 4.231e-78
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: A9NPK8_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 9.425e-78
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: D7M1A3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_908777 PE=4 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 2.100e-77
Identity = 140/142 (98.59%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: D7T753_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018060001 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 6.109e-77
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDI+ETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIVETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: C6T0C3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 6.109e-77
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDI+ETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIVETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: B9SUQ5_RICCO (Mitosis protein dim1, putative OS=Ricinus communis GN=RCOM_0626720 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 6.109e-77
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDI+ETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIVETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: A9PCP7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_813981 PE=2 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 6.109e-77
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDI+ETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIVETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: B7FMH0_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 7.979e-77
Identity = 140/142 (98.59%), Postives = 141/142 (99.30%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVM FFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMSFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: Q9FE62_ARATH (AT5g08290 OS=Arabidopsis thaliana GN=F8L15_20 PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.042e-76
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQ+ILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQSILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TrEMBL
Match: B8BHK6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_34094 PE=4 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.361e-76
Identity = 136/143 (95.10%), Postives = 143/143 (100.00%), Query Frame = -1

Query:  171 KMSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 599
            +MSY+LPHLHSGWAVDQAILAEEERLV+IRFGHDWDETCMQMDEVL++VAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDI+ETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:  132 EMSYLLPHLHSGWAVDQAILAEEERLVIIRFGHDWDETCMQMDEVLAAVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIVETVYRGARKGRGLVIAPKDYSTKYRY 274

[Close]

BLAST of GT620348 vs. SwissProt
Match: TXN4A_MOUSE (Thioredoxin-like protein 4A OS=Mus musculus GN=Txnl4a PE=2 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 1.636e-71
Identity = 124/142 (87.32%), Postives = 135/142 (95.07%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSYMLPHLH+GW VDQAIL+EE+R+VVIRFGHDWD TCM+MDEVL S+AE +KNFAVIYLVDITEVPDFN MYELYDP TVMFFFRNKHIMIDLGTGNNNKINWAM+DKQE +DIIETVYRGARKGRGLV++PKDYSTKYRY
Sbjct:    1 MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPTCMKMDEVLYSIAEKVKNFAVIYLVDITEVPDFNKMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWAMEDKQEMVDIIETVYRGARKGRGLVVSPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. SwissProt
Match: TXN4A_HUMAN (Thioredoxin-like protein 4A OS=Homo sapiens GN=TXNL4A PE=1 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 1.636e-71
Identity = 124/142 (87.32%), Postives = 135/142 (95.07%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSYMLPHLH+GW VDQAIL+EE+R+VVIRFGHDWD TCM+MDEVL S+AE +KNFAVIYLVDITEVPDFN MYELYDP TVMFFFRNKHIMIDLGTGNNNKINWAM+DKQE +DIIETVYRGARKGRGLV++PKDYSTKYRY
Sbjct:    1 MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPTCMKMDEVLYSIAEKVKNFAVIYLVDITEVPDFNKMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWAMEDKQEMVDIIETVYRGARKGRGLVVSPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. SwissProt
Match: DIMI_SCHPO (Mitosis protein dim1 OS=Schizosaccharomyces pombe GN=dim1 PE=1 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.466e-61
Identity = 108/142 (76.06%), Postives = 126/142 (88.73%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY LPHLHSGW VDQAIL+E+ERLVVIRFG D DE C++ DEVL  +AE + N AVIYLVDI EVPDFN MYELYD +T+MFF+RNKH+MIDLGTGNNNKINW ++DKQE IDIIET++RGARKG+GLVI+PKDYST++RY
Sbjct:    1 MSYFLPHLHSGWHVDQAILSEQERLVVIRFGRDHDEECIKQDEVLYRIAEKVVNMAVIYLVDIDEVPDFNKMYELYDRTTIMFFYRNKHMMIDLGTGNNNKINWPLEDKQEMIDIIETIFRGARKGKGLVISPKDYSTRHRY 142

[Close]

BLAST of GT620348 vs. SwissProt
Match: DIB1_ASHGO (Spliceosomal protein DIB1 OS=Ashbya gossypii GN=DIB1 PE=3 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 9.334e-51
Identity = 93/138 (67.39%), Postives = 112/138 (81.16%), Query Frame = -1

Query:  180 SYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTK 593
            S  LPHLHSGW VDQAI+ EEERLVVIRFG D D +CM MDE+L S+AE + NFA IY+ D TEVPDFN MYEL DP TVMFF++NKH+  D GTGNNNK+N+ + +KQE IDIIETV+RGAR+ +GLV++P DY+ K
Sbjct:    3 SVFLPHLHSGWHVDQAIVTEEERLVVIRFGSDSDRSCMLMDEILYSIAEKVVNFAAIYVCDTTEVPDFNEMYELQDPMTVMFFYKNKHMRCDFGTGNNNKMNFVVDNKQEMIDIIETVFRGARRNKGLVVSPYDYNYK 140

[Close]

BLAST of GT620348 vs. SwissProt
Match: TXN4A_DICDI (Thioredoxin-like protein 4A homolog OS=Dictyostelium discoideum GN=txnl4a PE=3 SV=4)

HSP 1 Score: 190.274 bits (482), Expect = 9.660e-48
Identity = 84/132 (63.64%), Postives = 112/132 (84.85%), Query Frame = -1

Query:  171 GWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 566
            G  + + +  EE+R+VVIRFGHD++  CM+ D++L+S+AE +KN AVIY+VDITEVPD N+MYELYD    MFF+RNKHIM+DLGTGNNNKINWA+ +KQ+ IDIIETVY+GARKG+GLV +P+D+S +Y++
Sbjct:    2 GGLLIKQLSTEEDRVVVIRFGHDYNPECMKQDDILASIAEKVKNMAVIYVVDITEVPDLNSMYELYDDCPTMFFYRNKHIMVDLGTGNNNKINWALTNKQDMIDIIETVYKGARKGKGLVNSPRDFSPQYKF 133

[Close]

BLAST of GT620348 vs. SwissProt
Match: DIB1_YEAST (Spliceosomal protein DIB1 OS=Saccharomyces cerevisiae GN=DIB1 PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.648e-47
Identity = 89/138 (64.49%), Postives = 110/138 (79.71%), Query Frame = -1

Query:  180 SYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTK 593
            S +LP L +GW VDQAI+ E +RLVVIRFG   D  CM MDE+LSS+AE ++NFAVIYL DI EV DF+ MYEL DP TVMFF+ NKH+M D GTGNNNK+N+ + DKQE IDI+ET++RGARK +GLV++P DY+ K
Sbjct:    3 SVLLPQLRTGWHVDQAIVTETKRLVVIRFGRKNDRQCMIMDELLSSIAERVRNFAVIYLCDIDEVSDFDEMYELTDPMTVMFFYHNKHMMCDFGTGNNNKLNFIVDDKQEMIDILETIFRGARKNKGLVVSPYDYNHK 140

[Close]

BLAST of GT620348 vs. SwissProt
Match: DIB1_CANGA (Spliceosomal protein DIB1 OS=Candida glabrata GN=DIB1 PE=3 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 4.058e-46
Identity = 86/135 (63.70%), Postives = 107/135 (79.26%), Query Frame = -1

Query:  180 LPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTK 584
            L +LHSGW VDQAI+ EEERLVV+RFG   D  CM MDE+L+SVAE ++NF  IYL DI EVPDFN MYEL D   +MFF++NKH+M D GTGNNNK+N+   DKQE IDI+ET++RGARK +G+V++P DY+ K
Sbjct:    4 LYNLHSGWHVDQAIVTEEERLVVVRFGRTADRECMLMDEMLASVAEKVRNFVAIYLCDIDEVPDFNDMYELNDNMCLMFFYKNKHMMCDFGTGNNNKMNFLPDDKQELIDIMETIFRGARKNKGIVVSPYDYNHK 138

[Close]

BLAST of GT620348 vs. SwissProt
Match: TXN4B_HUMAN (Thioredoxin-like protein 4B OS=Homo sapiens GN=TXNL4B PE=1 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.773e-25
Identity = 57/136 (41.91%), Postives = 90/136 (66.18%), Query Frame = -1

Query:  192 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYEL-YDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKD 596
            MS++LP L S   VDQAI +  E+++V+RFG D D  C+Q+D++LS  +  +   A IYLVD+ +   +   +++ Y PSTV FFF  +H+ +D G+ ++ K   + K KQ+FID+IE +YRGA +G+ +V +P D
Sbjct:    1 MSFLLPKLTSKKEVDQAIKSTAEKVLVLRFGRDEDPVCLQLDDILSKTSSDLSKMAAIYLVDVDQTAVYTQYFDISYIPSTV-FFFNGQHMKVDYGSPDHTKFVGSFKTKQDFIDLIEVIYRGAMRGKLIVQSPID 135

[Close]

BLAST of GT620348 vs. SwissProt
Match: TXN4B_MOUSE (Thioredoxin-like protein 4B OS=Mus musculus GN=Txnl4b PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 5.702e-24
Identity = 58/143 (40.56%), Postives = 89/143 (62.24%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYEL-YDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MS++LP L S   VDQAI +  E+ +V+RFG D D  C+Q+D++LS  +  +   A IYL D+   P +   +++ Y PSTV FFF  +H+  D G+ ++ K   + K KQ+FID+IE +YRGA +G+ +V +P D     +Y
Sbjct:    1 MSFLLPKLTSKKEVDQAIKSTAEKGLVLRFGRDEDPVCLQLDDILSKTSADLSKMAAIYLGDVDHTPVYTQYFDISYIPSTV-FFFNGQHMKGDYGSPDHTKFVGSFKTKQDFIDLIEVIYRGAMRGKLIVQSPIDPKNVPKY 142

[Close]

BLAST of GT620348 vs. TAIR peptide
Match: AT5G08290.1 (| Symbols: YLS8 | mRNA splicing factor, thioredoxin-like U5 snRNP | chr5:2666043-2666936 FORWARD LENGTH=142)

HSP 1 Score: 290.426 bits (742), Expect = 6.822e-79
Identity = 138/142 (97.18%), Postives = 142/142 (100.00%), Query Frame = -1

Query:  171 MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 596
            MSY+LPHLHSGWAVDQ+ILAEEERLVVIRFGHDWDETCMQMDEVL+SVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWA+KDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY
Sbjct:    1 MSYLLPHLHSGWAVDQSILAEEERLVVIRFGHDWDETCMQMDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWALKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY 142

[Close]

BLAST of GT620348 vs. TAIR peptide
Match: AT3G24730.1 (| Symbols: | mRNA splicing factor, thioredoxin-like U5 snRNP | chr3:9030152-9030894 REVERSE LENGTH=159)

HSP 1 Score: 90.8929 bits (224), Expect = 7.933e-19
Identity = 50/138 (36.23%), Postives = 79/138 (57.25%), Query Frame = -1

Query:  198 KMSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYD----PSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAP 599
            +MSY+L  L +   +D+ I    + ++V+RFG   D  C+Q DE+L+     +  FA + LVD+ +  D     + +D    PST+ FFF   H+ +D GT ++ K   A   KQ+FID++E +YRGA KG+ +V  P
Sbjct:    8 EMSYLLKTLTTKEEIDRVIRDTIDEVLVLRFGRSSDAVCLQHDEILAKSVRDVSKFAKVALVDV-DSEDVQVYVKYFDITLFPSTI-FFFNAHHMKLDSGTADHTKWVGAFHIKQDFIDVVEAIYRGAMKGKMIVQCP 143

The following BLAST results are available for this feature:

BLAST of GT620348 vs. TrEMBL
BLAST of GT620348 vs. SwissProt
BLAST of GT620348 vs. TAIR peptide

BLAST of GT620348 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FF76_MEDTR	4.231e-78	100.00	Putative uncharacterized protein OS=Medicago trunc...	[more]
A9NPK8_PICSI	9.425e-78	99.30	Putative uncharacterized protein OS=Picea sitchens...	[more]
D7M1A3_ARALY	2.100e-77	98.59	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7T753_VITVI	6.109e-77	97.18	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6T0C3_SOYBN	6.109e-77	97.18	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SUQ5_RICCO	6.109e-77	97.18	Mitosis protein dim1, putative OS=Ricinus communis...	[more]
A9PCP7_POPTR	6.109e-77	97.18	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B7FMH0_MEDTR	7.979e-77	98.59	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q9FE62_ARATH	1.042e-76	97.18	AT5g08290 OS=Arabidopsis thaliana GN=F8L15_20 PE=2...	[more]
B8BHK6_ORYSI	1.361e-76	95.10	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GT620348 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
TXN4A_MOUSE	1.636e-71	87.32	Thioredoxin-like protein 4A OS=Mus musculus GN=Txn...	[more]
TXN4A_HUMAN	1.636e-71	87.32	Thioredoxin-like protein 4A OS=Homo sapiens GN=TXN...	[more]
DIMI_SCHPO	4.466e-61	76.06	Mitosis protein dim1 OS=Schizosaccharomyces pombe ...	[more]
DIB1_ASHGO	9.334e-51	67.39	Spliceosomal protein DIB1 OS=Ashbya gossypii GN=DI...	[more]
TXN4A_DICDI	9.660e-48	63.64	Thioredoxin-like protein 4A homolog OS=Dictyosteli...	[more]
DIB1_YEAST	1.648e-47	64.49	Spliceosomal protein DIB1 OS=Saccharomyces cerevis...	[more]
DIB1_CANGA	4.058e-46	63.70	Spliceosomal protein DIB1 OS=Candida glabrata GN=D...	[more]
TXN4B_HUMAN	1.773e-25	41.91	Thioredoxin-like protein 4B OS=Homo sapiens GN=TXN...	[more]
TXN4B_MOUSE	5.702e-24	40.56	Thioredoxin-like protein 4B OS=Mus musculus GN=Txn...	[more]

BLAST of GT620348 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G08290.1	6.822e-79	97.18	\| Symbols: YLS8 \| mRNA splicing factor, thioredoxi...	[more]
AT3G24730.1	7.933e-19	36.23	\| Symbols: \| mRNA splicing factor, thioredoxin-li...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 75..88 score
None	No IPR available	SEG	seg	seg	coord: 22..34 score
None	No IPR available	SEG	seg	seg	coord: 4..14 score
IPR004123	mRNA splicing factor, thioredoxin-like U5 snRNP	PIR	PIRSF017199	Thioredoxin-like protein, 4A/4B type	coord: 5..147 score: 1.5e
IPR004123	mRNA splicing factor, thioredoxin-like U5 snRNP	PANTHER	PTHR12052	THIOREDOXIN-LIKE PROTEN 4A, 4B	coord: 6..147 score: 7.8e
IPR004123	mRNA splicing factor, thioredoxin-like U5 snRNP	PFAM	PF02966	DIM1	coord: 9..141 score: 2.9
IPR012335	Thioredoxin fold	GENE3D	3.40.30.10	no description	coord: 4..147 score: 7.9
IPR012336	Thioredoxin-like fold	SUPERFAMILY	52833	Thioredoxin-like	coord: 7..143 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620348 ID=GT620348; Name=GT620348; organism=Lens culinaris; type=EST; length=743bp
ATAAATTCCACACATCATAGCAGTTATTAGAGTTTAAGATGACAGACAAG
TATGTGTGTAAATCAGTTTAGTGATATTTGCACACTTCATACACAGGAAT
AAAAGATAGATTGAGGTGACATCACGATAACCAAATGAGGTAGCGTCCCA
ATAGAAATCGAGATATTCTAGTAACGATACTTGGTGGAGTAATCTTTGGG
AGCAATGACAAGACCGCGTCCCTTCCTTGCACCTCTGTAGACAGTTTCAA
TGATGTCAATGAACTCTTGTTTGTCCTTCATAGCCCAATTAATCTTGTTG
TTGTTTCCAGTTCCAAGGTCTATCATGATGTGCTTGTTCCTAAAGAAGAA
CATTACCGTTGAAGGATCGTACAGCTCATACATAGTGTTAAAATCAGGCA
CTTCTGTGATGTCCACAAGGTATATCACAGCAAAGTTCTTTATTGTTTCT
GCAACCGATGACAGCACCTCATCCATCTGCATACAAGTCTCGTCCCAATC
ATGTCCAAAACGGATGACAACGAGACGCTCTTCCTCCGCAAGGATTGCCT
GATCCACAGCCCACCCAGAATGCAAGTGAGGCAACATGTACGACATCTTT
CTTGTTCTTAATCACAAACTCTACTCTAGTGAGTAAGGAGAAGAACGAGT
TGAAGGTGGTGATGATTCGTGAAGTATCAAGTTTGCTCCCCAGAGAACTT
GACTAAGCTGTTGGTGAAGTCTGGGGTGACCCAACCAGCAACA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005681	spliceosomal complex

Vocabulary: **Biological Process**
Term	Definition
GO:0007067	mitosis

Vocabulary: **INTERPRO**
Term	Definition
IPR004123	Dim1
IPR012335	Thioredoxin fold
IPR012336	Thioredoxin-like_fold

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