GT626492

EST Overview


Name	GT626492
Unique Name	GT626492
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	737

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT626492 vs. TrEMBL
Match: B9GG35_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_797542 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 6.947e-33
Identity = 83/154 (53.90%), Postives = 112/154 (72.73%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLS--GKRRISVSKPNPI---PKEEMEKGSVDMSGSMTLLDFMGW-DDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI QSSLD+SKIQ+N+DVGHA+LESYSRILE+LA TVLSRI+DVL AD  T+N S  G +R ++ +   +   P+E MEK S D + SMTL DFMGW  +Q +S  KKD F  SD++ ++ + + ++KL  + TN+K SYL+ LG +RSP +RH
Sbjct:  298 GIPQSSLDVSKIQYNKDVGHAILESYSRILETLASTVLSRIEDVLDADYVTRNPSHAGHKRNTLKEAPQVPASPREGMEKNSEDTNASMTLSDFMGWGSEQNESAAKKDPFG-SDELLKD-DDRYKQKLTNISTNRKPSYLDNLGALRSPTARH 449

[Close]

BLAST of GT626492 vs. TrEMBL
Match: B9RQK7_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1493800 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.003e-31
Identity = 84/154 (54.55%), Postives = 112/154 (72.73%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLS--GKRRISVSK---PNPIPKEEMEKGSVDMSGSMTLLDFMGWD-DQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI QSSLDISKIQ+NRDVG A+LESYSRILESLA+TV+SRI+DVL AD  T+N S  G++R  + +    +  P+E+  + S D + SMTL DFMGW+ D  ++D KK     SD++ ++ +TKL +KL ++VT KK SYLE LG +RSP +RH
Sbjct:  403 GIPQSSLDISKIQYNRDVGQAILESYSRILESLAYTVMSRIEDVLYADYVTRNPSYAGQKRNPLKETPSESTTPREDSARTS-DAANSMTLSDFMGWNFDSNEADGKKGPAGNSDEIVKD-DTKLTEKLASIVTIKKPSYLENLGGVRSPTARH 554

[Close]

BLAST of GT626492 vs. TrEMBL
Match: B9GXB4_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_854418 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 4.979e-31
Identity = 83/154 (53.90%), Postives = 104/154 (67.53%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRIS-VSKPNP----IPKEEMEKGSVDMSGSMTLLDFMGW-DDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            G+ QSSLD+SKIQ+N DVGHA+LESYSRILE+LA TV+SRI+DVL AD   +N S     S   K  P     PKE MEK S D + SMTL DFMGW  +Q D+  KKD F  SD++ ++ + K  +KL  + TN+K SYL+ LG  RSP +RH
Sbjct:  303 GLPQSSLDVSKIQYNEDVGHAILESYSRILETLASTVMSRIEDVLYADHVARNPSHAGHTSNTLKEAPQVLVSPKEGMEKNSEDTNASMTLSDFMGWSQEQNDTTAKKDPFG-SDELLKD-DDKCTQKLANISTNRKPSYLDNLGASRSPTARH 454

[Close]

BLAST of GT626492 vs. TrEMBL
Match: Q5DK69_SOLLC (Pollen-specific kinase partner protein OS=Solanum lycopersicum GN=KPP PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 1.226e-29
Identity = 84/157 (53.50%), Postives = 104/157 (66.24%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADS---NTQNLSGKR-----RISVSKPNPIPKEEMEK-GSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI QSSLDISKIQ+NRD+G A+LESYSRI+ES AFTV+SRI+DV+QAD    +  N   KR      + VS      KEE+EK  S +   SMTLLDFMGW  + D D +KD+ E  +   +E + KL  K P +VTNK+VSYLE L   RSP +RH
Sbjct:  349 GIPQSSLDISKIQYNRDIGQAILESYSRIIESRAFTVMSRIEDVMQADDLAHDPSNAEVKRFPMGDSLGVSDGMFNDKEEVEKLSSAETPNSMTLLDFMGW-GEGDEDTEKDTKE--EIRSKESDAKLLSKPPNIVTNKRVSYLENLTGSRSPTARH 502

[Close]

BLAST of GT626492 vs. TrEMBL
Match: D7KXP2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_477280 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.356e-28
Identity = 85/152 (55.92%), Postives = 99/152 (65.13%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWD-DQ---PDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI+QS+LDISKIQFN+DVG AVLESYSRILESLA+TVLSRIDDVL+AD      +G +R             ME     + GSMTL DFMGWD DQ    D D KKD   +SDD        L K+  +VVT KK SYLETLG ++SP +RH
Sbjct:  389 GISQSALDISKIQFNKDVGQAVLESYSRILESLAYTVLSRIDDVLEADR-----AGNKR----------NTPMESEDETLVGSMTLSDFMGWDFDQAGNADLDSKKD---LSDD-------PLVKEKLSVVTTKKTSYLETLGGVKSPTARH 515

[Close]

BLAST of GT626492 vs. TrEMBL
Match: D7U0I0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017070001 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.313e-28
Identity = 79/152 (51.97%), Postives = 103/152 (67.76%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWD-DQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSY---LETLGVMRSPESRH 737
            G+ QS LDI+KIQFN+DVGHA+LESYSRILE+LAFTVLSRI+DVL +  + + L+           P PKEE+EK S     SMTL DFMGW+ ++ D + KK S   S+++ +  E K   K+  ++TNKKVSY   LE LG +RSP +RH
Sbjct:  294 GLDQSQLDINKIQFNQDVGHAILESYSRILETLAFTVLSRIEDVLGSSLDPEPLN----------FPNPKEEIEKLSTGTPQSMTLSDFMGWNLEKEDGEEKKGSGGNSEELSKNDE-KPMTKIANIITNKKVSYIEKLENLGGLRSPTARH 434

[Close]

BLAST of GT626492 vs. TrEMBL
Match: Q9CA89_ARATH (At1g79860 OS=Arabidopsis thaliana GN=At1g79860 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.958e-27
Identity = 80/149 (53.69%), Postives = 95/149 (63.76%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWD-DQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI+QSSLDISKIQFN DVG AVLESYSRILESLA+TVLSRIDDVL+AD          R    +  P+  EE       + GSMTL DFMGWD DQ  +   +   ++ DD        L K+  +VVT KK SYLETLG ++SP +RH
Sbjct:  389 GISQSSLDISKIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD----------RAGNKRNTPLEAEE-----ETLVGSMTLSDFMGWDFDQAANAELESKKDLPDD-------PLIKEKLSVVTTKKTSYLETLGGVKSPTARH 515

[Close]

BLAST of GT626492 vs. TrEMBL
Match: D7KIQ8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_337379 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 3.692e-26
Identity = 81/153 (52.94%), Postives = 103/153 (67.32%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQ----NLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVT-NKKVSYLETLGVMRSPESRH 737
            GI+QSSLDISKIQFNRDVG A+LESYSR+LESLA TV+SRI+DVL AD  TQ    N   K R S+ K N   +EE    + DM+ S TL D M W ++ +++MKK+SF      + + E  L  K+  ++T NKK SYLE LG MRSP +R+
Sbjct:  394 GISQSSLDISKIQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSL-KENEKLREERLTFTEDMA-SGTLSDVMQWGNK-NNEMKKESF------FGDREKPLLSKVTGIMTNNKKSSYLENLGAMRSPTARY 537

[Close]

BLAST of GT626492 vs. TrEMBL
Match: Q9M811_ARATH (Putative uncharacterized protein F9I5.12 OS=Arabidopsis thaliana GN=At1g52240 PE=4 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 6.298e-26
Identity = 80/153 (52.29%), Postives = 103/153 (67.32%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQ----NLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVT-NKKVSYLETLGVMRSPESRH 737
            GI+QSSLDISKIQFNRDVG A+LESYSR+LESLA TV+SRI+DVL AD  TQ    N   K R S+ K N   +EE    + DM+ S TL D M W ++ +++MKK+SF      + + E  L  K+  ++T NKK SYL+ LG MRSP +R+
Sbjct:  395 GISQSSLDISKIQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSL-KENEKLREERLSFTEDMA-SGTLSDVMQWGNK-NNEMKKESF------FGDREKPLLSKVTGIMTNNKKSSYLDNLGAMRSPTARY 538

[Close]

BLAST of GT626492 vs. TrEMBL
Match: C5XZZ8_SORBI (Putative uncharacterized protein Sb04g010270 OS=Sorghum bicolor GN=Sb04g010270 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 5.332e-25
Identity = 78/158 (49.37%), Postives = 100/158 (63.29%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLS---GKRRISVSKPNPI-----PKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETL--GVMRSPESRH 737
            G  QS LDISKIQ+NRDVG+A+LESYSR+LESLA++V+SRI+DVL AD+  QNL+     RR+  S   P       KEE+EK + +   SMTL DFMGW    D  MK+      D    + E  L KK P++   KK SY+++L  G MRSP +RH
Sbjct:  367 GTPQSQLDISKIQYNRDVGYALLESYSRVLESLAYSVMSRIEDVLSADAAAQNLTATEAARRMMESAELPAARKLDAKEELEKLN-EAPASMTLFDFMGWHFDQDELMKRREDGTLD---ADGEAMLLKKAPSMAP-KKFSYVDSLAAGGMRSPSARH 519

[Close]

BLAST of GT626492 vs. SwissProt
Match: ROGF1_ARATH (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 5.453e-11
Identity = 32/64 (50.00%), Postives = 50/64 (78.12%), Query Frame = -1

Query:  546 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSK 737
            G+ Q++LD++KIQ+N+DVG ++LESYSR++ES+AF + +RIDDVL  D      + +R ISV++
Sbjct:  405 GLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDD-----AMRRSISVTE 463

[Close]

BLAST of GT626492 vs. SwissProt
Match: ROGF2_ARATH (Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana GN=ROPGEF2 PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 2.992e-9
Identity = 30/47 (63.83%), Postives = 38/47 (80.85%), Query Frame = -1

Query:  594 IAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQAD 734
            ++Q+SLDI KI  N+DVG AVLESYSR+LE LAF +++ IDDVL  D
Sbjct:  432 LSQTSLDICKIHCNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVD 478

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT1G79860.1 (| Symbols: ATROPGEF12, ROPGEF12, MEE64 | RHO guanyl-nucleotide exchange factor 12 | chr1:30042172-30044092 REVERSE LENGTH=515)

HSP 1 Score: 126.716 bits (317), Expect = 1.287e-29
Identity = 80/149 (53.69%), Postives = 95/149 (63.76%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWD-DQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI+QSSLDISKIQFN DVG AVLESYSRILESLA+TVLSRIDDVL+AD          R    +  P+  EE       + GSMTL DFMGWD DQ  +   +   ++ DD        L K+  +VVT KK SYLETLG ++SP +RH
Sbjct:  389 GISQSSLDISKIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD----------RAGNKRNTPLEAEE-----ETLVGSMTLSDFMGWDFDQAANAELESKKDLPDD-------PLIKEKLSVVTTKKTSYLETLGGVKSPTARH 515

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT1G52240.1 (| Symbols: ATROPGEF11, ROPGEF11, PIRF1 | RHO guanyl-nucleotide exchange factor 11 | chr1:19455766-19459235 REVERSE LENGTH=607)

HSP 1 Score: 121.709 bits (304), Expect = 4.140e-28
Identity = 80/153 (52.29%), Postives = 103/153 (67.32%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQ----NLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVT-NKKVSYLETLGVMRSPESRH 737
            GI+QSSLDISKIQFNRDVG A+LESYSR+LESLA TV+SRI+DVL AD  TQ    N   K R S+ K N   +EE    + DM+ S TL D M W ++ +++MKK+SF      + + E  L  K+  ++T NKK SYL+ LG MRSP +R+
Sbjct:  463 GISQSSLDISKIQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSL-KENEKLREERLSFTEDMA-SGTLSDVMQWGNK-NNEMKKESF------FGDREKPLLSKVTGIMTNNKKSSYLDNLGAMRSPTARY 606

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT3G16130.1 (| Symbols: ATROPGEF13, ROPGEF13, PIRF2 | RHO guanyl-nucleotide exchange factor 13 | chr3:5466246-5468512 FORWARD LENGTH=576)

HSP 1 Score: 101.679 bits (252), Expect = 4.434e-22
Identity = 69/157 (43.95%), Postives = 94/157 (59.87%), Query Frame = -1

Query:  306 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLS-GKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVET------KLEKKLPAVVT------NKKVSYLETLGVMRSP 737
            GI+QS+LDISKIQFNRD+G A++ESYSRILESLA TV+SRI+DVL+AD  TQN      +I + K    P++E E             +F   +D+P    +K +  +S+ +   +ET      K +KKL   V+      NKK +YLE+LG  RSP
Sbjct:  428 GISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRIEDVLEADQLTQNPELAMCKIHIVKETESPEKEEEP------------NFCLLEDRPKK--QKPTISLSEVMQWNIETNEPRKEKSDKKLLTRVSSMIMSNNKKTTYLESLGTTRSP 570

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT5G19560.1 (| Symbols: ATROPGEF10, ROPGEF10 | ROP uanine nucleotide exchange factor 10 | chr5:6603291-6606130 FORWARD LENGTH=493)

HSP 1 Score: 101.293 bits (251), Expect = 5.791e-22
Identity = 69/153 (45.10%), Postives = 89/153 (58.17%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQ-NLSGKRRISVSKPNPIPKEEMEK-GSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEK---KLPAVVTNKKVSYLETLGVMRSPESRH 737
            GI QSSL++SKI+ N+DVG A+LESYSR+LESLA  ++SRI+DVL+AD   Q  L G+             EE EK  + +   S  L DF+GW  +  SD KK S     + + +VE + EK   K P  +  KK SYL  L  MRSP  RH
Sbjct:  344 GIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLEADRLVQRQLMGEAETRSESEAESEYEETEKVVAAETPNSRKLSDFIGW--RLSSDTKKHSSMSDIEFFHKVEQEKEKPMMKSPRALP-KKFSYLAKLENMRSPSDRH 493

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT4G13240.1 (| Symbols: ATROPGEF9, ROPGEF9 | RHO guanyl-nucleotide exchange factor 9 | chr4:7680153-7682414 FORWARD LENGTH=517)

HSP 1 Score: 100.523 bits (249), Expect = 9.877e-22
Identity = 70/163 (42.94%), Postives = 87/163 (53.37%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADS------NTQNLSGKRRISVSKPNP------IPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSY---LETLGVMRSPESRH 737
            G+ QSSLDISKIQ+N+DVGHAVLESYSRILESL +T +SRIDDVL ADS        +  S   +I+    +          EE+EK     S   TLLDF+GW D                     +++   K P  +T KK+SY   LE L   RSP+ RH
Sbjct:  372 GLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARKQCTGEETSDGGKIATETDSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGWSDNSSKG----------------QSEKPPKSPR-MTPKKLSYLEKLENLNGFRSPKDRH 517

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT3G24620.1 (| Symbols: ATROPGEF8, ROPGEF8 | RHO guanyl-nucleotide exchange factor 8 | chr3:8980695-8982793 REVERSE LENGTH=523)

HSP 1 Score: 97.8265 bits (242), Expect = 6.402e-21
Identity = 68/159 (42.77%), Postives = 82/159 (51.57%), Query Frame = -1

Query:  294 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADS--------NTQNLSGKRRISVSKPNPIPKEEMEKGSVDMSGSMTLLDFMGWDDQPDSDMKKDSFEISDDVYQEVETKLEKKLPAVVTNKKVSY---LETLGVMRSPESRH 737
            G+ QSSLDISKIQFN+DVG AVLESYSRILESLA+TV+SRI+DVL  D+          +   G R       +       E+       S TLLDFMGW+D       K +                 K P  +T KK+SY   LE L   RSP+ RH
Sbjct:  383 GLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGWNDNSSKGGDKPT-----------------KSPN-LTPKKLSYLEKLENLNGFRSPKDRH 523

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT2G45890.1 (| Symbols: ATROPGEF4, ROPGEF4, RHS11 | RHO guanyl-nucleotide exchange factor 4 | chr2:18883540-18885440 FORWARD LENGTH=463)

HSP 1 Score: 71.2478 bits (173), Expect = 6.417e-13
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = -1

Query:  579 IAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQN 734
            ++Q+SLDI KIQFNRDVG AVLESYSR+LE LA+ V+S IDDVL  D   +N
Sbjct:  409 LSQTSLDICKIQFNRDVGKAVLESYSRVLEGLAYNVVSWIDDVLYVDRTVRN 460

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT5G02010.1 (| Symbols: ATROPGEF7, ROPGEF7 | RHO guanyl-nucleotide exchange factor 7 | chr5:383493-385698 FORWARD LENGTH=546)

HSP 1 Score: 70.4774 bits (171), Expect = 1.095e-12
Identity = 31/55 (56.36%), Postives = 47/55 (85.45%), Query Frame = -1

Query:  573 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLS 737
            G+ Q++LD+SKIQ+N+D+G ++LESYSR+LESLAF +++RIDD+L  D  T++ S
Sbjct:  383 GLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVARIDDLLFVDDLTRHSS 437

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT4G38430.1 (| Symbols: ATROPGEF1, ROPGEF1 | rho guanyl-nucleotide exchange factor 1 | chr4:17986643-17988659 FORWARD LENGTH=548)

HSP 1 Score: 68.1662 bits (165), Expect = 5.433e-12
Identity = 32/64 (50.00%), Postives = 50/64 (78.12%), Query Frame = -1

Query:  546 GIAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQADSNTQNLSGKRRISVSK 737
            G+ Q++LD++KIQ+N+DVG ++LESYSR++ES+AF + +RIDDVL  D      + +R ISV++
Sbjct:  405 GLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDD-----AMRRSISVTE 463

[Close]

BLAST of GT626492 vs. TAIR peptide
Match: AT4G00460.2 (| Symbols: ATROPGEF3, ROPGEF3 | RHO guanyl-nucleotide exchange factor 3 | chr4:211146-213094 REVERSE LENGTH=473)

HSP 1 Score: 66.2402 bits (160), Expect = 2.064e-11
Identity = 31/47 (65.96%), Postives = 40/47 (85.11%), Query Frame = -1

Query:  594 IAQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVLSRIDDVLQAD 734
            ++Q+SLDI KIQ+N+DVG AVLESYSR+LE LAF +++ IDDVL  D
Sbjct:  420 LSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVD 466

The following BLAST results are available for this feature:

BLAST of GT626492 vs. TrEMBL
BLAST of GT626492 vs. SwissProt
BLAST of GT626492 vs. TAIR peptide

BLAST of GT626492 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GG35_POPTR	6.947e-33	53.90	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RQK7_RICCO	1.003e-31	54.55	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9GXB4_POPTR	4.979e-31	53.90	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q5DK69_SOLLC	1.226e-29	53.50	Pollen-specific kinase partner protein OS=Solanum ...	[more]
D7KXP2_ARALY	1.356e-28	55.92	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7U0I0_VITVI	2.313e-28	51.97	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9CA89_ARATH	1.958e-27	53.69	At1g79860 OS=Arabidopsis thaliana GN=At1g79860 PE=...	[more]
D7KIQ8_ARALY	3.692e-26	52.94	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9M811_ARATH	6.298e-26	52.29	Putative uncharacterized protein F9I5.12 OS=Arabid...	[more]
C5XZZ8_SORBI	5.332e-25	49.37	Putative uncharacterized protein Sb04g010270 OS=So...	[more]

BLAST of GT626492 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
ROGF1_ARATH	5.453e-11	50.00	Rop guanine nucleotide exchange factor 1 OS=Arabid...	[more]
ROGF2_ARATH	2.992e-9	63.83	Rop guanine nucleotide exchange factor 2 OS=Arabid...	[more]

BLAST of GT626492 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G79860.1	1.287e-29	53.69	\| Symbols: ATROPGEF12, ROPGEF12, MEE64 \| RHO guany...	[more]
AT1G52240.1	4.140e-28	52.29	\| Symbols: ATROPGEF11, ROPGEF11, PIRF1 \| RHO guany...	[more]
AT3G16130.1	4.434e-22	43.95	\| Symbols: ATROPGEF13, ROPGEF13, PIRF2 \| RHO guany...	[more]
AT5G19560.1	5.791e-22	45.10	\| Symbols: ATROPGEF10, ROPGEF10 \| ROP uanine nucle...	[more]
AT4G13240.1	9.877e-22	42.94	\| Symbols: ATROPGEF9, ROPGEF9 \| RHO guanyl-nucleot...	[more]
AT3G24620.1	6.402e-21	42.77	\| Symbols: ATROPGEF8, ROPGEF8 \| RHO guanyl-nucleot...	[more]
AT2G45890.1	6.417e-13	67.31	\| Symbols: ATROPGEF4, ROPGEF4, RHS11 \| RHO guanyl-...	[more]
AT5G02010.1	1.095e-12	56.36	\| Symbols: ATROPGEF7, ROPGEF7 \| RHO guanyl-nucleot...	[more]
AT4G38430.1	5.433e-12	50.00	\| Symbols: ATROPGEF1, ROPGEF1 \| rho guanyl-nucleot...	[more]
AT4G00460.2	2.064e-11	65.96	\| Symbols: ATROPGEF3, ROPGEF3 \| RHO guanyl-nucleot...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005512	Rop nucleotide exchanger, PRONE	PFAM	PF03759	PRONE	coord: 1..53 score: 1.1
IPR005512	Rop nucleotide exchanger, PRONE	PROFILE	PS51334	PRONE	coord: 1..58 score: 21

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT626492 ID=GT626492; Name=GT626492; organism=Lens culinaris; type=EST; length=737bp
ATACAATGTTGTTGCTGTTATTTTCAATTGAATCAAAGATAATCATGTCT
ACATAAAAATTATTACCACAAAACAATAGAAAAGATACATTGTGGTGTCT
TAAAAAATTCTCAAATAGGTAGCTACCTACCAGACTACAAATCCTTCAGA
GGAAGATATATCTAGGACAAATGTTTCGAATGGTTCTGCCATCCCTATGT
ACAAAATTAGATAATGGATCAAGAATTAACAATGAAGATGACTCCAACAA
AATTATACACCTCTATGAGCCTAAATATAGCGTCATTGTCTTAATGACGC
GACTCGGGACTCCTCATGACTCCCAAGGTTTCAAGGTATGAAACTTTCTT
GTTGGTCACTACGGCCGGAAGCTTTTTTTCCAGCTTCGTTTCGACTTCTT
GGTAAACATCGTCTGAAATTTCAAAAGAGTCCTTCTTCATGTCTGAGTCG
GGTTGATCATCCCAGCCCATGAAATCCAATAGTGTCATCGAACCAGACAT
GTCTACGCTGCCTTTCTCCATCTCTTCTTTTGGAATAGGATTTGGCTTCG
AAACTGATATTCTCCTTTTTCCTGATAGATTTTGAGTGTTAGAATCGGCT
TGAAGTACATCGTCTATTCTCGATAGTACTGTGAATGCCAAACTTTCCAA
TATTCTAGAATAGCTTTCAAGAACAGCGTGCCCTACATCCCTGTTGAACT
GGATTTTGCTTATATCCAATGAAGATTGAGCAATGCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005089	Rho guanyl-nucleotide exchange factor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005512	PRONE_dom

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