GT627433

EST Overview


Name	GT627433
Unique Name	GT627433
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	383

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT627433 vs. TrEMBL
Match: B7FGM9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.303e-27
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS 173
            KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS
Sbjct:  133 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS 189

[Close]

BLAST of GT627433 vs. TrEMBL
Match: C6TN19_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.128e-26
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS 173
            KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA AGLFPMPDPKTS
Sbjct:  133 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGASAGLFPMPDPKTS 189

[Close]

BLAST of GT627433 vs. TrEMBL
Match: D7TIB6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033465001 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.928e-24
Identity = 54/58 (93.10%), Postives = 56/58 (96.55%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRG-AGAGLFPMPDPKTS 173
            KMSHRGL GNDPTDCSMAFLYFLCSISIRTNLQKFLGF+PPRG AGAGLFPMPDPKT+
Sbjct:  136 KMSHRGLNGNDPTDCSMAFLYFLCSISIRTNLQKFLGFSPPRGAAGAGLFPMPDPKTN 193

[Close]

BLAST of GT627433 vs. TrEMBL
Match: B9SWJ7_RICCO (Transmembrane and coiled-coil domains protein, putative OS=Ricinus communis GN=RCOM_0279040 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.479e-23
Identity = 53/58 (91.38%), Postives = 56/58 (96.55%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRG-AGAGLFPMPDPKTS 173
            KMSHRGLQG+D TDCSMAFLYFLCSISIRTNLQKFLGF+PPRG AGAGLFPMPDPKT+
Sbjct:  135 KMSHRGLQGDDATDCSMAFLYFLCSISIRTNLQKFLGFSPPRGAAGAGLFPMPDPKTN 192

[Close]

BLAST of GT627433 vs. TrEMBL
Match: Q4TZT9_GOSHI (Fb27 OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.740e-22
Identity = 51/57 (89.47%), Postives = 54/57 (94.74%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRG-AGAGLFPMPDPKT 170
            KMSHRGL+G+D TDCSM FLYFLCSISIRTNLQKFLGF+PPRG AGAGLFPMPDPKT
Sbjct:  137 KMSHRGLKGDDSTDCSMVFLYFLCSISIRTNLQKFLGFSPPRGAAGAGLFPMPDPKT 193

[Close]

BLAST of GT627433 vs. TrEMBL
Match: Q9LHL8_ARATH (Genomic DNA, chromosome 3, P1 clone: MEC18 OS=Arabidopsis thaliana GN=At3g12030/T21B14_117 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.973e-22
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+DPTDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDPTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. TrEMBL
Match: Q8LE05_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.973e-22
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+DPTDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDPTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. TrEMBL
Match: D7KZN5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478555 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.973e-22
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+DPTDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDPTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. TrEMBL
Match: Q9FG04_ARATH (AT5g06660/F15M7_19 OS=Arabidopsis thaliana GN=At5g06660 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.750e-21
Identity = 49/59 (83.05%), Postives = 53/59 (89.83%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+D TDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDSTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. TrEMBL
Match: Q8LDF3_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.750e-21
Identity = 49/59 (83.05%), Postives = 53/59 (89.83%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+D TDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDSTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_RAT (Transmembrane and coiled-coil domains protein 1 OS=Rattus norvegicus GN=Tmco1 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.469e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDP 164
            +SHR L G+D TDCS  FLY LC++SIR N+QK LG AP R A   AG F  P P
Sbjct:  128 LSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPP 182

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_PONAB (Transmembrane and coiled-coil domain-containing protein 1 OS=Pongo abelii GN=TMCO1 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.469e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDP 164
            +SHR L G+D TDCS  FLY LC++SIR N+QK LG AP R A   AG F  P P
Sbjct:  128 LSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPP 182

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_MOUSE (Transmembrane and coiled-coil domain-containing protein 1 OS=Mus musculus GN=Tmco1 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.469e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDP 164
            +SHR L G+D TDCS  FLY LC++SIR N+QK LG AP R A   AG F  P P
Sbjct:  128 LSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPP 182

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_HUMAN (Transmembrane and coiled-coil domain-containing protein 1 OS=Homo sapiens GN=TMCO1 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.469e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDP 164
            +SHR L G+D TDCS  FLY LC++SIR N+QK LG AP R A   AG F  P P
Sbjct:  128 LSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPP 182

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_BOVIN (Transmembrane and coiled-coil domain-containing protein 1 OS=Bos taurus GN=TMCO1 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.469e-10
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDP 164
            +SHR L G+D TDCS  FLY LC++SIR N+QK LG AP R A   AG F  P P
Sbjct:  128 LSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPP 182

[Close]

BLAST of GT627433 vs. SwissProt
Match: TMCO1_DICDI (Transmembrane and coiled-coil domain-containing protein 1 homolog OS=Dictyostelium discoideum GN=tmco1 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.152e-9
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 3

Query:    6 MSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPK 167
            +SHR + G+D TDCSM F+Y +CS+ IR N+Q  LG APP+   A  + +P+ K
Sbjct:  130 ISHRTIAGDDYTDCSMTFIYAICSMFIRNNIQLILGTAPPKTKQANPWALPEEK 183

[Close]

BLAST of GT627433 vs. TAIR peptide
Match: AT3G12030.1 (| Symbols: | Protein of unknown function DUF106, transmembrane | chr3:3834465-3835055 REVERSE LENGTH=196)

HSP 1 Score: 106.686 bits (265), Expect = 3.774e-24
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+DPTDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDPTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

[Close]

BLAST of GT627433 vs. TAIR peptide
Match: AT5G06660.1 (| Symbols: | Protein of unknown function DUF106, transmembrane | chr5:2046863-2047453 FORWARD LENGTH=196)

HSP 1 Score: 103.605 bits (257), Expect = 3.195e-23
Identity = 49/59 (83.05%), Postives = 53/59 (89.83%), Query Frame = 3

Query:    3 KMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGA--GAGLFPMPDPKTS 173
            KMSHRGL+G+D TDCSMAFLY LCSISIRTNLQKFLGF+PPRGA    GLFPMPDPKT+
Sbjct:  138 KMSHRGLKGDDSTDCSMAFLYLLCSISIRTNLQKFLGFSPPRGAAGAGGLFPMPDPKTN 196

The following BLAST results are available for this feature:

BLAST of GT627433 vs. TrEMBL
BLAST of GT627433 vs. SwissProt
BLAST of GT627433 vs. TAIR peptide

BLAST of GT627433 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FGM9_MEDTR	6.303e-27	100.00	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TN19_SOYBN	3.128e-26	98.25	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TIB6_VITVI	2.928e-24	93.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SWJ7_RICCO	2.479e-23	91.38	Transmembrane and coiled-coil domains protein, put...	[more]
Q4TZT9_GOSHI	2.740e-22	89.47	Fb27 OS=Gossypium hirsutum PE=2 SV=1	[more]
Q9LHL8_ARATH	7.973e-22	84.75	Genomic DNA, chromosome 3, P1 clone: MEC18 OS=Arab...	[more]
Q8LE05_ARATH	7.973e-22	84.75	Putative uncharacterized protein OS=Arabidopsis th...	[more]
D7KZN5_ARALY	7.973e-22	84.75	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9FG04_ARATH	6.750e-21	83.05	AT5g06660/F15M7_19 OS=Arabidopsis thaliana GN=At5g...	[more]
Q8LDF3_ARATH	6.750e-21	83.05	Putative uncharacterized protein OS=Arabidopsis th...	[more]

BLAST of GT627433 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
TMCO1_RAT	6.469e-10	58.18	Transmembrane and coiled-coil domains protein 1 OS...	[more]
TMCO1_PONAB	6.469e-10	58.18	Transmembrane and coiled-coil domain-containing pr...	[more]
TMCO1_MOUSE	6.469e-10	58.18	Transmembrane and coiled-coil domain-containing pr...	[more]
TMCO1_HUMAN	6.469e-10	58.18	Transmembrane and coiled-coil domain-containing pr...	[more]
TMCO1_BOVIN	6.469e-10	58.18	Transmembrane and coiled-coil domain-containing pr...	[more]
TMCO1_DICDI	7.152e-9	48.15	Transmembrane and coiled-coil domain-containing pr...	[more]

BLAST of GT627433 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G12030.1	3.774e-24	84.75	\| Symbols: \| Protein of unknown function DUF106, ...	[more]
AT5G06660.1	3.195e-23	83.05	\| Symbols: \| Protein of unknown function DUF106, ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002809	Protein of unknown function DUF106, transmembrane	PFAM	PF01956	DUF106	coord: 2..40 score: 7.1
IPR008559	Uncharacterised conserved protein UCP023322, transmembrane eukaryotic	PANTHER	PTHR20917	PNAS-RELATED	coord: 1..57 score: 5.3
None	No IPR available	SEG	seg	seg	coord: 17..31 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627433 ID=GT627433; Name=GT627433; organism=Lens culinaris; type=EST; length=383bp
TGAAGATGAGCCATCGAGGATTGCAAGGTAATGATCCCACTGATTGTTCT
ATGGCATTTCTATACTTCTTGTGTTCCATTAGTATCAGAACAAATTTGCA
GAAGTTCTTGGGTTTTGCACCGCCAAGGGGTGCAGGTGCTGGTCTCTTTC
CAATGCCAGACCCCAAGACCAGTTGATCTAGCCTTGTTATCTTAGCATTT
TATCTCGCTATTTCTAGGTTGATGTTAAACTGGAACTGCATAACTTTAGT
TATTTATATTTCATTCGGTTCTCTTAGGGAGACCAGCATGATTTTAGTTG
TTTTAACTGATACTGAAGAAGTCAACTTCAGTTTTATGATGGTTATTTGC
TTGTTTTTAAGTACAATAAAGAGTTTCTTTGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR002809	EMC3/TMCO1
IPR008559	TMCO1

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