GT620547

EST Overview


Name	GT620547
Unique Name	GT620547
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	767

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT620547 vs. TrEMBL
Match: A0FK64_MEDTR (PHD4 (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.578e-17
Identity = 46/65 (70.77%), Postives = 47/65 (72.31%), Query Frame = 1

Query:    1 SNESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            +NESW                  GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   52 TNESWEVTLPAEEVPPELPEPALGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: C6TE22_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 7.501e-17
Identity = 45/64 (70.31%), Postives = 46/64 (71.88%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NE+W                  GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   53 NETWEVTLPAEEVPPELPEPALGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: C6T7X8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 7.501e-17
Identity = 45/64 (70.31%), Postives = 46/64 (71.88%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NE+W                  GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   53 NETWEVTLPAEEVPPELPEPALGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: C6TCB0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.671e-16
Identity = 44/64 (68.75%), Postives = 46/64 (71.88%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NE+W                  GINFARDGMNRRDWLSLVAVHSDSWL+SVAFYLGARLNRNER
Sbjct:   82 NEAWEVTLPAEEVPPELPEPALGINFARDGMNRRDWLSLVAVHSDSWLVSVAFYLGARLNRNER 145

[Close]

BLAST of GT620547 vs. TrEMBL
Match: A9PGU1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_560917 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.671e-16
Identity = 44/64 (68.75%), Postives = 46/64 (71.88%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NE+W                  GINFARDGMNR+DWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   53 NETWEVTLPAEEVPPELPEPALGINFARDGMNRKDWLSLVAVHSDSWLLSVAFYLGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: B9IGA0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_750025 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.350e-16
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 1

Query:   70 GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            GINFARDGMNR+DWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   73 GINFARDGMNRKDWLSLVAVHSDSWLLSVAFYLGARLNRNER 114

[Close]

BLAST of GT620547 vs. TrEMBL
Match: A9PA67_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.350e-16
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 1

Query:   70 GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            GINFARDGMNR+DWLSLVAVHSDSWLLSVAFYLGARLNRNER
Sbjct:   75 GINFARDGMNRKDWLSLVAVHSDSWLLSVAFYLGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: D7SY14_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_105.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011162001 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.083e-15
Identity = 42/64 (65.62%), Postives = 45/64 (70.31%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            N++W                  GINFARDGMNR+DWLSLVAVHSDSWLLS AFYLGARLNRNER
Sbjct:   54 NDTWEVTLPAEEVPPELPEPALGINFARDGMNRKDWLSLVAVHSDSWLLSAAFYLGARLNRNER 117

[Close]

BLAST of GT620547 vs. TrEMBL
Match: B9SQ16_RICCO (Phd/F-box containing protein, putative OS=Ricinus communis GN=RCOM_0643160 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.415e-15
Identity = 42/64 (65.62%), Postives = 45/64 (70.31%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NESW                  GINFARDGM+R+DWLSLVAVHSDSWL+SVAFY GARLNRNER
Sbjct:   53 NESWEVNLPAEEVPPELPEPALGINFARDGMDRKDWLSLVAVHSDSWLISVAFYFGARLNRNER 116

[Close]

BLAST of GT620547 vs. TrEMBL
Match: Q6Q7P5_SOLLC (Nucleic acid-binding protein OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 2.413e-15
Identity = 42/64 (65.62%), Postives = 44/64 (68.75%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NESW                  GINFARDGM RRDWLSLVA+H+DSWLLSVAFY GARLNRNER
Sbjct:   56 NESWEVAVPAEEVPPELPEPVLGINFARDGMERRDWLSLVAMHTDSWLLSVAFYFGARLNRNER 119

[Close]

BLAST of GT620547 vs. SwissProt
Match: Y5621_ARATH (PHD finger protein At5g26210 OS=Arabidopsis thaliana GN=At5g26210 PE=1 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 9.899e-11
Identity = 32/58 (55.17%), Postives = 36/58 (62.07%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR 177
            NE W                  GINFARDGM  +DWLSLVAVHSD+WLL+VAF+ GAR
Sbjct:   55 NEHWEVNLPAEEVPPELPEPVLGINFARDGMAEKDWLSLVAVHSDAWLLAVAFFFGAR 112

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT3G11200.2 (| Symbols: AL2 | alfin-like 2 | chr3:3508387-3510195 REVERSE LENGTH=233)

HSP 1 Score: 84.7297 bits (208), Expect = 5.990e-17
Identity = 41/64 (64.06%), Postives = 43/64 (67.19%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NESW                  GINFARDGM R+DWLSLVAVHSD WLLSV+FY GARLNRNER
Sbjct:   43 NESWEVNLPAEEVPPELPEPALGINFARDGMQRKDWLSLVAVHSDCWLLSVSFYFGARLNRNER 106

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT3G11200.1 (| Symbols: AL2 | alfin-like 2 | chr3:3508387-3510418 REVERSE LENGTH=246)

HSP 1 Score: 84.7297 bits (208), Expect = 5.990e-17
Identity = 41/64 (64.06%), Postives = 43/64 (67.19%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            NESW                  GINFARDGM R+DWLSLVAVHSD WLLSV+FY GARLNRNER
Sbjct:   56 NESWEVNLPAEEVPPELPEPALGINFARDGMQRKDWLSLVAVHSDCWLLSVSFYFGARLNRNER 119

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT5G05610.2 (| Symbols: AL1 | alfin-like 1 | chr5:1677331-1678942 REVERSE LENGTH=241)

HSP 1 Score: 78.5666 bits (192), Expect = 4.293e-15
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 1

Query:   70 GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            GINFARDGM R+DWLSLVAVHSD WLLSV+ Y GARLNRNER
Sbjct:   76 GINFARDGMQRKDWLSLVAVHSDCWLLSVSSYFGARLNRNER 117

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT5G05610.1 (| Symbols: AL1 | alfin-like 1 | chr5:1677331-1678942 REVERSE LENGTH=241)

HSP 1 Score: 78.5666 bits (192), Expect = 4.293e-15
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 1

Query:   70 GINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNER 195
            GINFARDGM R+DWLSLVAVHSD WLLSV+ Y GARLNRNER
Sbjct:   76 GINFARDGMQRKDWLSLVAVHSDCWLLSVSSYFGARLNRNER 117

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT2G02470.2 (| Symbols: AL6 | alfin-like 6 | chr2:652837-654621 FORWARD LENGTH=247)

HSP 1 Score: 73.559 bits (179), Expect = 1.381e-13
Identity = 37/66 (56.06%), Postives = 41/66 (62.12%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR--LNRNER 195
            NE+W                  GINFARDGM  +DW+SLVAVHSDSWLLSVAFY GAR    +NER
Sbjct:   55 NETWEVNLPVEEVPPELPEPALGINFARDGMQEKDWVSLVAVHSDSWLLSVAFYFGARFGFGKNER 120

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT2G02470.1 (| Symbols: AL6 | alfin-like 6 | chr2:652837-654621 FORWARD LENGTH=256)

HSP 1 Score: 73.559 bits (179), Expect = 1.381e-13
Identity = 37/66 (56.06%), Postives = 41/66 (62.12%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR--LNRNER 195
            NE+W                  GINFARDGM  +DW+SLVAVHSDSWLLSVAFY GAR    +NER
Sbjct:   55 NETWEVNLPVEEVPPELPEPALGINFARDGMQEKDWVSLVAVHSDSWLLSVAFYFGARFGFGKNER 120

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT1G14510.1 (| Symbols: AL7 | alfin-like 7 | chr1:4962171-4964154 REVERSE LENGTH=252)

HSP 1 Score: 73.1738 bits (178), Expect = 1.804e-13
Identity = 36/66 (54.55%), Postives = 41/66 (62.12%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR--LNRNER 195
            NE+W                  GINFARDGM  +DW+SLVAVHSDSWL+SVAFY GAR    +NER
Sbjct:   55 NETWEVNLPVEEVPPELPEPALGINFARDGMQEKDWISLVAVHSDSWLISVAFYFGARFGFGKNER 120

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT5G20510.1 (| Symbols: AL5 | alfin-like 5 | chr5:6939991-6942846 REVERSE LENGTH=260)

HSP 1 Score: 67.3958 bits (163), Expect = 9.897e-12
Identity = 32/58 (55.17%), Postives = 36/58 (62.07%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR 177
            NE W                  GINFARDGM  R+WLSLVAVHSD+WLLSV+FY G+R
Sbjct:   56 NEVWEVNLPAEEVPPELPEPALGINFARDGMQERNWLSLVAVHSDAWLLSVSFYFGSR 113

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT5G26210.1 (| Symbols: AL4 | alfin-like 4 | chr5:9158566-9160221 REVERSE LENGTH=255)

HSP 1 Score: 67.3958 bits (163), Expect = 9.897e-12
Identity = 32/58 (55.17%), Postives = 36/58 (62.07%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGAR 177
            NE W                  GINFARDGM  +DWLSLVAVHSD+WLL+VAF+ GAR
Sbjct:   55 NEHWEVNLPAEEVPPELPEPVLGINFARDGMAEKDWLSLVAVHSDAWLLAVAFFFGAR 112

[Close]

BLAST of GT620547 vs. TAIR peptide
Match: AT3G42790.1 (| Symbols: AL3 | alfin-like 3 | chr3:14878128-14879618 REVERSE LENGTH=250)

HSP 1 Score: 66.6254 bits (161), Expect = 1.688e-11
Identity = 29/60 (48.33%), Postives = 38/60 (63.33%), Query Frame = 1

Query:    4 NESWXXXXXXXXXXXXXXXXXXGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLN 183
            NE W                  GINFARDG++ ++WLSLVA+HSD+WLLSV+FY G+R +
Sbjct:   56 NEEWEVNLPAEEVPPELPEPALGINFARDGLSEKEWLSLVAIHSDAWLLSVSFYFGSRFS 115

The following BLAST results are available for this feature:

BLAST of GT620547 vs. TrEMBL
BLAST of GT620547 vs. SwissProt
BLAST of GT620547 vs. TAIR peptide

BLAST of GT620547 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A0FK64_MEDTR	2.578e-17	70.77	PHD4 (Fragment) OS=Medicago truncatula PE=2 SV=1	[more]
C6TE22_SOYBN	7.501e-17	70.31	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T7X8_SOYBN	7.501e-17	70.31	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TCB0_SOYBN	1.671e-16	68.75	Putative uncharacterized protein OS=Glycine max PE...	[more]
A9PGU1_POPTR	1.671e-16	68.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IGA0_POPTR	6.350e-16	97.62	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PA67_POPTR	6.350e-16	97.62	Putative uncharacterized protein OS=Populus tricho...	[more]
D7SY14_VITVI	1.083e-15	65.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SQ16_RICCO	1.415e-15	65.63	Phd/F-box containing protein, putative OS=Ricinus ...	[more]
Q6Q7P5_SOLLC	2.413e-15	65.63	Nucleic acid-binding protein OS=Solanum lycopersic...	[more]

BLAST of GT620547 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
Y5621_ARATH	9.899e-11	55.17	PHD finger protein At5g26210 OS=Arabidopsis thalia...	[more]

BLAST of GT620547 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G11200.2	5.990e-17	64.06	\| Symbols: AL2 \| alfin-like 2 \| chr3:3508387-35101...	[more]
AT3G11200.1	5.990e-17	64.06	\| Symbols: AL2 \| alfin-like 2 \| chr3:3508387-35104...	[more]
AT5G05610.2	4.293e-15	85.71	\| Symbols: AL1 \| alfin-like 1 \| chr5:1677331-16789...	[more]
AT5G05610.1	4.293e-15	85.71	\| Symbols: AL1 \| alfin-like 1 \| chr5:1677331-16789...	[more]
AT2G02470.2	1.381e-13	56.06	\| Symbols: AL6 \| alfin-like 6 \| chr2:652837-654621...	[more]
AT2G02470.1	1.381e-13	56.06	\| Symbols: AL6 \| alfin-like 6 \| chr2:652837-654621...	[more]
AT1G14510.1	1.804e-13	54.55	\| Symbols: AL7 \| alfin-like 7 \| chr1:4962171-49641...	[more]
AT5G20510.1	9.897e-12	55.17	\| Symbols: AL5 \| alfin-like 5 \| chr5:6939991-69428...	[more]
AT5G26210.1	9.897e-12	55.17	\| Symbols: AL4 \| alfin-like 4 \| chr5:9158566-91602...	[more]
AT3G42790.1	1.688e-11	48.33	\| Symbols: AL3 \| alfin-like 3 \| chr3:14878128-1487...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021998	Protein of unknown function DUF3594	PFAM	PF12165	DUF3594	coord: 2..65 score: 1.8
None	No IPR available	SEG	seg	seg	coord: 6..23 score
None	No IPR available	SEG	seg	seg	coord: 33..45 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620547 ID=GT620547; Name=GT620547; organism=Lens culinaris; type=EST; length=767bp
TCAAATGAATCATGGGAAGTGACTCTTCCAGCAGAGGAGGTTCCACCTGA
GCTTCCAGAGCCAGCTCTTGGAATCAATTTTGCAAGGGATGGCATGAACC
GTAGGGATTGGCTTTCTCTTGTTGCTGTGCACAGTGATTCATGGTTGCTT
TCTGTGGCTTTTTATCTTGGAGCTCGTCTTAACCGCAATGAAAGGTAGTA
CTATTTACAGGGTATAAGTATGTGGTTATTATAATTTTTGTTGGTTTTGG
CTCATTATATGTAATTCACATGCTTAAGAAGCACGGTGCTAAATGTTGAG
AACCGAGGCCTGCAATTGTAGTAGCTGAGTTGCATCAATATCATCATTGT
TATCTGTTGGGCGGTGATTCTGGATAAGTTTGTGTATATATGTTCCTTGG
ATGGGTTAAGACCATTTTTTATTTATCATCCATAAGTTACAATTTAGGGA
AGTTGATAATTAACTTAAGGTTGTGTCTAAGGTGACTCTGTTGTAACGTT
TAGATATAACTTAGAATTGAACAATTATGTTGAATAACTTATGTATTTTA
TCTCTCCCCATTGGTACTCCCCCTCCTTACCACACTGTGTTCGGAAGGGA
ATTACAGGCTTCATAGATTGTTGAGGGCAAATACCTGCAACTAAGTTTGA
TTTATCATTTGTATGGTAGAATGTGTTACTGTTGCTCTGGTCTTGCTGTT
TCTCCCTTATAACTTTCCCATTCTAATGAGCTTATTTGTTTTAGCGTTTC
CGTCAACTGGTTTGTAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR021998	Alfin_N

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