GT624734

EST Overview


Name	GT624734
Unique Name	GT624734
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	701

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT624734 vs. TrEMBL
Match: B9RK58_RICCO (Ectonucleotide pyrophosphatase/phosphodiesterase, putative OS=Ricinus communis GN=RCOM_1046730 PE=4 SV=1)

HSP 1 Score: 355.525 bits (911), Expect = 2.314e-96
Identity = 158/201 (78.61%), Postives = 180/201 (89.55%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPLTKL HPVVLLISSDGFRFGYQFKT TP+I RLI+NGTEAETGL+PVFP+LTFPNHYSI TGLYPAYHGIINNHF+DP +G+ F MGSH+PKWWLGEP+W+TV N+GLKA+TYFWPGSEV+KG+W+CP  +C+ YNGSV FE RVD +L +FD  S+EIP FMTLYFEDPDHQGH+VG DDPEIT+AV RID MIGRLI
Sbjct:   92 RPLTKLNHPVVLLISSDGFRFGYQFKTPTPNIHRLINNGTEAETGLVPVFPTLTFPNHYSIVTGLYPAYHGIINNHFVDPNTGEHFTMGSHEPKWWLGEPLWETVANHGLKAATYFWPGSEVHKGSWDCPEDFCMFYNGSVPFEERVDKILSYFDLPSNEIPVFMTLYFEDPDHQGHQVGPDDPEITEAVGRIDRMIGRLI 292

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BLAST of GT624734 vs. TrEMBL
Match: B9H8W6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_653311 PE=4 SV=1)

HSP 1 Score: 348.206 bits (892), Expect = 3.694e-94
Identity = 158/201 (78.61%), Postives = 175/201 (87.06%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPLTKL H VVLLISSDGFRFGYQFKT TP+I RLI+NGTEAETGLIPVFPSLTFPNHYSI TGLYPAYHGIINNHF+DP +G+ F M SH+PKWWLGEPIW+TV   GLKASTYFWPGSEV+KG+W CP K+C+ YNGSV F+ RVD+VL +FD    EIP FMTLYFEDPDHQGHKVG DDPEIT+AV+ ID MIG+LI
Sbjct:   99 RPLTKLNHSVVLLISSDGFRFGYQFKTPTPNIHRLIANGTEAETGLIPVFPSLTFPNHYSIVTGLYPAYHGIINNHFVDPKTGEVFTMASHEPKWWLGEPIWETVAKQGLKASTYFWPGSEVHKGSWTCPQKFCMFYNGSVPFDERVDTVLSYFDLPDSEIPVFMTLYFEDPDHQGHKVGPDDPEITEAVAGIDRMIGKLI 299

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BLAST of GT624734 vs. TrEMBL
Match: Q9SU83_ARATH (Nucleotide pyrophosphatase-like protein OS=Arabidopsis thaliana GN=T16L4.190 PE=2 SV=1)

HSP 1 Score: 340.502 bits (872), Expect = 7.702e-92
Identity = 153/201 (76.12%), Postives = 176/201 (87.56%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPL KL  PVVLLISSDGFRFGYQFKT  PSI RLI+NGTEAETGLIPVFP+LTFPNHYSI TGLYPAYHGIINNHF+DP +G+ F M SH+P+WWLGEP+W+TVVN GLKA+TYFWPGSEV+KG+WNCP   C +YNGSV F++RVD++L +FD  S+EIP FMTLYFEDPDHQGH+VG DDP+IT+AV  ID +IGRLI
Sbjct:   99 RPLKKLDKPVVLLISSDGFRFGYQFKTKLPSIHRLIANGTEAETGLIPVFPTLTFPNHYSIVTGLYPAYHGIINNHFVDPETGNVFTMASHEPEWWLGEPLWETVVNQGLKAATYFWPGSEVHKGSWNCPQGLCQNYNGSVPFDDRVDTILSYFDLPSNEIPSFMTLYFEDPDHQGHQVGPDDPQITEAVVNIDRLIGRLI 299

[Close]

BLAST of GT624734 vs. TrEMBL
Match: D7MCJ0_ARALY (Type I phosphodiesterase/nucleotide pyrophosphatase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_491794 PE=4 SV=1)

HSP 1 Score: 335.109 bits (858), Expect = 3.236e-90
Identity = 152/201 (75.62%), Postives = 172/201 (85.57%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPL KL  PVVLLISSDGFRFGYQFKT  PSI RLI+NGTEAETGLIPVFP+LTFPNHYSI TGLYPAYHGIINN F DP +G+ F M SH+P+WWLGEP+W+TVVN GLKA+TYFWPGSEV+KG+WNCP   C  YNGSV F++RVD++L +FD  S EIP FMTLYFEDPDHQGH+VG DDP+IT+AV  ID +IGRLI
Sbjct:   99 RPLKKLDKPVVLLISSDGFRFGYQFKTKLPSIHRLIANGTEAETGLIPVFPTLTFPNHYSIVTGLYPAYHGIINNRFFDPETGNVFTMASHEPEWWLGEPLWETVVNQGLKAATYFWPGSEVHKGSWNCPQGLCQSYNGSVPFDDRVDTILSYFDLPSSEIPSFMTLYFEDPDHQGHQVGPDDPQITEAVVNIDRLIGRLI 299

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BLAST of GT624734 vs. TrEMBL
Match: Q9FS13_SPIOL (Nucleotide pyrophosphatase-like protein OS=Spinacia oleracea GN=Spnplp-1 PE=2 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 1.606e-89
Identity = 147/201 (73.13%), Postives = 172/201 (85.57%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            R LTKL HP+V+LISSDGFRFGYQFK  TP+I RLI+NGT AE GLIPVFP+LTFPNHYSI TGLYP +HGIINN+F+DP++GD+F M SH+PKWWLGEP+W+TV  +G KA+TYFWPGSEV K  W+CPS YC+HYNGS+ FE RVD+V+ +FD   +EIP FMTLYFEDPDHQGH+VG DDPEIT+AV  ID MIGRLI
Sbjct:   86 RSLTKLTHPIVILISSDGFRFGYQFKAPTPNIHRLINNGTSAELGLIPVFPTLTFPNHYSIVTGLYPPHHGIINNYFVDPITGDKFSMASHEPKWWLGEPLWETVAKHGKKAATYFWPGSEVIKPDWDCPSNYCIHYNGSIPFEERVDTVISYFDLPINEIPVFMTLYFEDPDHQGHQVGPDDPEITQAVINIDKMIGRLI 286

[Close]

BLAST of GT624734 vs. TrEMBL
Match: B9N5F9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_828210 PE=4 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 1.606e-89
Identity = 151/201 (75.12%), Postives = 173/201 (86.07%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            R LTKL HPVVLLISSDGFRFGYQFKT TP+I RLI NGTEAETGLIPVFP+LTFPNHYSI TGLYPA+HGIINN+F DP +G+ F M SH+PKWWLGEP+W+TV N+GLKA+TYFWPGSEV+KG+W CP  +C+ YNGSV F+ RVD+VL +FD  + EIP FMTLYFEDPDHQGH VG D PEIT+AV+ ID MIG+LI
Sbjct:   89 RSLTKLNHPVVLLISSDGFRFGYQFKTHTPNIHRLIVNGTEAETGLIPVFPTLTFPNHYSIVTGLYPAHHGIINNYFDDPKTGEVFTMASHEPKWWLGEPLWETVANHGLKAATYFWPGSEVHKGSWTCPPGFCMFYNGSVPFDERVDTVLSYFDLPASEIPVFMTLYFEDPDHQGHNVGPDGPEITEAVAGIDRMIGKLI 289

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BLAST of GT624734 vs. TrEMBL
Match: Q7F5L2_ORYSJ (Os01g0196600 protein OS=Oryza sativa subsp. japonica GN=P0419B01.3 PE=4 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 3.349e-87
Identity = 144/201 (71.64%), Postives = 168/201 (83.58%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            R   KL+ PVV+LISSDGFRFGYQ K +TP I RLI NGT A TGL+P+FP+LTFPNHYSIATGLYP+ HGIINN+F DP+SGD F M SH+PKWWLGEP+W T  + G++A+TYFWPGSEV KG+W+CP KYC HYNGSV FE RVD++L +FD  SDE+P F+TLYFEDPDHQGH+VG DDP IT+AV RID MIGRLI
Sbjct:   82 RAQVKLEKPVVILISSDGFRFGYQHKAATPHIHRLIGNGTSAATGLVPIFPTLTFPNHYSIATGLYPSSHGIINNYFPDPISGDYFTMSSHEPKWWLGEPLWVTAADQGIQAATYFWPGSEVKKGSWDCPDKYCRHYNGSVPFEERVDAILGYFDLPSDEMPQFLTLYFEDPDHQGHQVGPDDPAITEAVVRIDEMIGRLI 282

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BLAST of GT624734 vs. TrEMBL
Match: Q42974_ORYSA (Nucleotide pyrophosphatase OS=Oryza sativa GN=OSE4 PE=2 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 3.349e-87
Identity = 144/201 (71.64%), Postives = 168/201 (83.58%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            R   KL+ PVV+LISSDGFRFGYQ K +TP I RLI NGT A TGL+P+FP+LTFPNHYSIATGLYP+ HGIINN+F DP+SGD F M SH+PKWWLGEP+W T  + G++A+TYFWPGSEV KG+W+CP KYC HYNGSV FE RVD++L +FD  SDE+P F+TLYFEDPDHQGH+VG DDP IT+AV RID MIGRLI
Sbjct:   82 RAQVKLEKPVVILISSDGFRFGYQHKAATPHIHRLIGNGTSAATGLVPIFPTLTFPNHYSIATGLYPSSHGIINNYFPDPISGDYFTMSSHEPKWWLGEPLWVTAADQGIQAATYFWPGSEVKKGSWDCPDKYCRHYNGSVPFEERVDAILGYFDLPSDEMPQFLTLYFEDPDHQGHQVGPDDPAITEAVVRIDEMIGRLI 282

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BLAST of GT624734 vs. TrEMBL
Match: B8ADR2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00764 PE=4 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 3.349e-87
Identity = 144/201 (71.64%), Postives = 168/201 (83.58%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            R   KL+ PVV+LISSDGFRFGYQ K +TP I RLI NGT A TGL+P+FP+LTFPNHYSIATGLYP+ HGIINN+F DP+SGD F M SH+PKWWLGEP+W T  + G++A+TYFWPGSEV KG+W+CP KYC HYNGSV FE RVD++L +FD  SDE+P F+TLYFEDPDHQGH+VG DDP IT+AV RID MIGRLI
Sbjct:   82 RAQVKLEKPVVILISSDGFRFGYQHKAATPHIHRLIGNGTSAATGLVPIFPTLTFPNHYSIATGLYPSSHGIINNYFPDPISGDYFTMSSHEPKWWLGEPLWVTAADQGIQAATYFWPGSEVKKGSWDCPDKYCRHYNGSVPFEERVDAILGYFDLPSDEMPQFLTLYFEDPDHQGHQVGPDDPAITEAVVRIDEMIGRLI 282

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BLAST of GT624734 vs. TrEMBL
Match: C5XLV5_SORBI (Putative uncharacterized protein Sb03g002880 OS=Sorghum bicolor GN=Sb03g002880 PE=4 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 2.653e-84
Identity = 142/201 (70.65%), Postives = 164/201 (81.59%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPL+KL  PVVLLISSDGFRFGYQ+K   P I RL +NGT A  GLIPVFP+LTFPNHYSI TGLYP+ HGIINN+F DP+SGD F M +HDPK+WLGEP+W+T  N GL A+T+FWPGSEV KG+WNCP KYC  YNGSV FE RVD++L +FD  SD++P F+TLYFEDPDHQGH+VG DDP IT AV  ID M+GRLI
Sbjct:   73 RPLSKLPKPVVLLISSDGFRFGYQYKAPLPHIRRLFANGTSAAEGLIPVFPTLTFPNHYSIVTGLYPSSHGIINNYFPDPISGDYFNMRNHDPKFWLGEPLWETAANQGLLAATFFWPGSEVKKGSWNCPEKYCQLYNGSVPFEERVDTILGYFDLPSDQMPQFLTLYFEDPDHQGHQVGPDDPAITDAVVHIDEMLGRLI 273

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BLAST of GT624734 vs. SwissProt
Match: ENPP1_HUMAN (Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 OS=Homo sapiens GN=ENPP1 PE=1 SV=2)

HSP 1 Score: 163.696 bits (413), Expect = 8.772e-40
Identity = 91/199 (45.73%), Postives = 117/199 (58.79%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGYQFKTS--TPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHD---PKWWLGEPIWKTVVNNGLKASTYFWPGSEVN-KGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P  LL S DGFR  Y        P I++L   GT  +  + PV+P+ TFPNHYSI TGLYP  HGII+N   DP     F + S +   P+W+ GEPIW T    GLK+ T+FWPGS+V   G +  P  Y + YNGSV FE R+ +VL++     DE P F TLY E+PD  GH  G    E+ KA+ R+D M+G L+
Sbjct:  210 PPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKN-MRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIF--PDIYKM-YNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLM 404

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BLAST of GT624734 vs. SwissProt
Match: ENPP3_HUMAN (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 OS=Homo sapiens GN=ENPP3 PE=1 SV=2)

HSP 1 Score: 160.614 bits (405), Expect = 7.426e-39
Identity = 87/199 (43.72%), Postives = 122/199 (61.31%), Query Frame = 2

Query:  119 PVVLLISSDGFR--FGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGS---HDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVN-KGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P V+L S DGFR  + Y + T  P+I +L + G  ++  +  ++P+ TFPNHY+I TGLYP  HGII+N+  D      F + S   ++P WW G+P+W T +  GLKA+TYFWPGSEV   G++  PS Y + YNGSV FE R+ ++LK+ D    E P F T+YFE+PD  GH  G     + KA+  +D   G L+
Sbjct:  159 PPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKY-MRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSF--PSIY-MPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLM 353

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP3_BOVIN (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 OS=Bos taurus GN=ENPP3 PE=1 SV=2)

HSP 1 Score: 160.229 bits (404), Expect = 9.699e-39
Identity = 87/199 (43.72%), Postives = 119/199 (59.80%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGY--QFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHD---PKWWLGEPIWKTVVNNGLKASTYFWPGSEVN-KGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P V+L S DGFR  Y   + T  P+I +L + G  ++  L P +P+ TFPNHY+I TGLYP  HGII+N+  D      F + S +   P WW G+PIW T +  GLK  TYFWPGS+V   GT+  PS Y + YN SV++E R+ ++LK+ D    E PDF T+Y E+PD QGH  G     + +A+  +D   G L+
Sbjct:  159 PPVILFSMDGFRAEYLQTWSTLVPNINKLKTCGVHSQY-LRPAYPTKTFPNHYTIVTGLYPESHGIIDNNMYDINLNKNFSLSSKEKDNPAWWQGQPIWLTAMYQGLKVGTYFWPGSDVAINGTF--PSIYKI-YNRSVTYEERIFTLLKWLDLPKAERPDFYTIYVEEPDSQGHNYGPVSAGVIQALQLVDKTFGLLM 353

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP3_PONAB (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 OS=Pongo abelii GN=ENPP3 PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 2.389e-37
Identity = 85/199 (42.71%), Postives = 119/199 (59.80%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGY--QFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGS---HDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVN-KGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P V+L S DGFR  Y   + T  P+I +L + G  ++  +  ++P+ TFPNHY+I  GLYP  HGII+N+  D      F + S   ++P WW G+P+W T +  GLKA+TYFWPGSE    G++  PS Y + YNGSV FE R+ ++LK+ D    E P F T+YFE+PD  GH  G     + KA+  +D   G L+
Sbjct:  159 PPVILFSMDGFRAEYLHTWDTLMPNINKLKTCGIHSKY-MRAMYPTKTFPNHYTIVMGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEAAINGSF--PSIY-MPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDFSGHAGGPVSARVIKALQIVDHAFGMLM 353

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP1_MOUSE (Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 OS=Mus musculus GN=Enpp1 PE=1 SV=4)

HSP 1 Score: 152.91 bits (385), Expect = 1.548e-36
Identity = 85/202 (42.08%), Postives = 117/202 (57.92%), Query Frame = 2

Query:  107 KLKHPVVLLISSDGFRFGYQFKTS--TPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHD---PKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            + + P  LL S DGFR  Y        P I++L + GT  +  + P++P+ TFPNHYSI TGLYP  HGII+N   DP     F + S +   P W+ G+PIW T  +  +K+ TYFWPGS+V       P  Y + YNGSV FE R+ +VL++    S E P F TLY E+PD  GH  G    E+ KA+ ++D ++G L+
Sbjct:  188 EFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKN-MRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGI-LPDIYKV-YNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLM 386

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP1_RAT (Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 OS=Rattus norvegicus GN=Enpp1 PE=2 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 3.450e-36
Identity = 85/202 (42.08%), Postives = 117/202 (57.92%), Query Frame = 2

Query:  107 KLKHPVVLLISSDGFRFGYQFKTS--TPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHD---PKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            + + P  LL S DGFR  Y        P I++L + GT  +  + PV+P+ TFPNHYSI TGLYP  HGII+N   DP     F + S +   P W+ G+PIW T  +  +++ TYFWPGS+V       P  Y + YNGSV FE R+ +VL++    S E P F TLY E+PD  GH  G    E+ KA+ ++D ++G L+
Sbjct:  188 EFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKN-MRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVRSGTYFWPGSDVEIDGI-LPDIYKV-YNGSVPFEERILAVLEWLQLPSYERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDHIVGMLM 386

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP3_RAT (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 OS=Rattus norvegicus GN=Enpp3 PE=1 SV=2)

HSP 1 Score: 149.058 bits (375), Expect = 2.236e-35
Identity = 79/198 (39.90%), Postives = 118/198 (59.60%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGY--QFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGS---HDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P V+L S DGFR  Y   + T  P+I +L + G  ++  +  ++P+ TFPNHY+I TGLYP  HGII+N+  D      F + S    +P WW G+PIW T +  GLKA++Y+WPGS+V     + P+ Y  +Y+ SV +E+R+ ++L++ D    E P F T+Y E+PD  GHK G     + KA+  +D   G L+
Sbjct:  160 PPVILFSMDGFRAEYLQTWSTLLPNINKLKTCGLHSKY-MRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVYLNKNFSLSSVEKSNPAWWSGQPIWLTAMYQGLKAASYYWPGSDVAVNG-SFPNIY-RNYSNSVPYESRIATLLQWLDLPKAERPSFYTIYVEEPDSAGHKSGPVSAGVIKALQLVDDAFGMLM 354

[Close]

BLAST of GT624734 vs. SwissProt
Match: YGK5_SCHPO (Uncharacterized pyrophosphatase/phosphodiesterase C725.05c OS=Schizosaccharomyces pombe GN=SPBC725.05c PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 8.497e-35
Identity = 75/203 (36.95%), Postives = 108/203 (53.20%), Query Frame = 2

Query:  104 TKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMG----SHDPKWW-LGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            T +    V++IS DGFR  Y ++  TP++  L          LIP FPS+TFPNHY+I TGLYP  HGI++N+F DPV+G QF       + DP WW  GEPIW     N ++++ + WPG+EV    +     Y   +N   +   + D +L++ D    + P  +  Y    D  GH  G D PE+   +  +D +IG LI
Sbjct:   69 TNVFQSTVIVISLDGFRADYLYRGFTPNLLSLAERNVHVPF-LIPSFPSITFPNHYTIVTGLYPESHGIVSNNFFDPVTGKQFVNSMPECNKDPTWWDKGEPIWVNAERNNVRSAVHMWPGNEVENHGYR--PTYSDGFNFDTTLREKKDRILEWLDLPDKDRPQLLLAYAPHVDMVGHAFGPDSPELNIIIQEVDIVIGELI 268

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP3_MOUSE (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 OS=Mus musculus GN=Enpp3 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.602e-33
Identity = 76/198 (38.38%), Postives = 115/198 (58.08%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGY--QFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGS---HDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P V+L S DGFR  Y   + T  P+I +L + G  ++  +  ++P+ TFPNHY+  TGLYP  HGII+N+  D      F + S    +P WW G+PIW T +  GLKA+ Y+WPGS+V     + P+ Y  +Y+ SV +E R+ ++L++ D    + P F T+Y E+PD  GH  G     + KA+  +D+  G L+
Sbjct:  159 PPVILFSMDGFRAEYLQTWSTLLPNINKLKTCGIHSKY-MRAMYPTKTFPNHYTSVTGLYPESHGIIDNNMYDVHLNKNFSLSSVEKSNPAWWSGQPIWLTAMYQGLKAACYYWPGSDVAVNG-SFPTIY-RNYSNSVPYERRITTLLQWLDLPKADRPSFYTIYVEEPDSAGHSSGPVSAGVIKALQSVDNAFGMLM 353

[Close]

BLAST of GT624734 vs. SwissProt
Match: ENPP2_RAT (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 OS=Rattus norvegicus GN=Enpp2 PE=1 SV=2)

HSP 1 Score: 129.798 bits (325), Expect = 1.404e-29
Identity = 70/198 (35.35%), Postives = 103/198 (52.02%), Query Frame = 2

Query:  119 PVVLLISSDGFRFGYQFKTST--PSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDP---KWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P +++ S DGFR  Y  K S   P+I +L S GT A   + PV+P+ TFPN Y++ATGLYP  HGI+ N   DPV    F++   +    +WW G+P+W T    G++A T+FW                    + S+  E R+ ++L++     +E P     Y E PD  GHK G   PE+T  +  ID  +G+L+
Sbjct:  163 PPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPY-MRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDASFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFW--------------------SVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLM 339

[Close]

BLAST of GT624734 vs. TAIR peptide
Match: AT4G29680.1 (| Symbols: | Alkaline-phosphatase-like family protein | chr4:14538067-14539557 REVERSE LENGTH=496)

HSP 1 Score: 340.502 bits (872), Expect = 5.264e-94
Identity = 153/201 (76.12%), Postives = 176/201 (87.56%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            RPL KL  PVVLLISSDGFRFGYQFKT  PSI RLI+NGTEAETGLIPVFP+LTFPNHYSI TGLYPAYHGIINNHF+DP +G+ F M SH+P+WWLGEP+W+TVVN GLKA+TYFWPGSEV+KG+WNCP   C +YNGSV F++RVD++L +FD  S+EIP FMTLYFEDPDHQGH+VG DDP+IT+AV  ID +IGRLI
Sbjct:   99 RPLKKLDKPVVLLISSDGFRFGYQFKTKLPSIHRLIANGTEAETGLIPVFPTLTFPNHYSIVTGLYPAYHGIINNHFVDPETGNVFTMASHEPEWWLGEPLWETVVNQGLKAATYFWPGSEVHKGSWNCPQGLCQNYNGSVPFDDRVDTILSYFDLPSNEIPSFMTLYFEDPDHQGHQVGPDDPQITEAVVNIDRLIGRLI 299

[Close]

BLAST of GT624734 vs. TAIR peptide
Match: AT4G29700.1 (| Symbols: | Alkaline-phosphatase-like family protein | chr4:14543739-14545124 REVERSE LENGTH=461)

HSP 1 Score: 291.197 bits (744), Expect = 3.662e-79
Identity = 135/200 (67.50%), Postives = 152/200 (76.00%), Query Frame = 2

Query:   98 PLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYCLHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            P  KL  PVVL+IS DGFRFGYQFKT TP+I  LIS GTEA+ GLIPVFP++TFPNHYSIATGLYPAYHGII N F DPV+G+ F  G   PKWWLGEP+W T VN GLKA TYFWPGSEV K +W CP  YC H+N SV  E RVDSVL  FD   DE+PD + LYF++PD  GH  G DDP +T AVSR+D MIGR+I
Sbjct:   51 PFKKLNKPVVLMISCDGFRFGYQFKTDTPNIDLLISGGTEAKHGLIPVFPTMTFPNHYSIATGLYPAYHGIIMNKFTDPVTGEVFNKGLQ-PKWWLGEPLWVTAVNQGLKAVTYFWPGSEVLKSSWTCPEGYCPHFNLSVPLEERVDSVLSHFDHLEDEVPDLLMLYFDEPDQSGHNYGPDDPRVTTAVSRVDKMIGRVI 249

[Close]

BLAST of GT624734 vs. TAIR peptide
Match: AT4G29690.1 (| Symbols: | Alkaline-phosphatase-like family protein | chr4:14541084-14542457 REVERSE LENGTH=457)

HSP 1 Score: 287.73 bits (735), Expect = 4.049e-78
Identity = 134/202 (66.34%), Postives = 160/202 (79.21%), Query Frame = 2

Query:   95 RPLTKLKHPVVLLISSDGFRFGYQFKTSTPSITRLISNGTEAETGLIPVFPSLTFPNHYSIATGLYPAYHGIINNHFIDPVSGDQFYMGSHDPKWWLGEPIWKTVVNNGLKASTYFWPGSEVNKGTWNCPSKYC-LHYNGSVSFENRVDSVLKFFDFASDEIPDFMTLYFEDPDHQGHKVGSDDPEITKAVSRIDSMIGRLI 697
            +P  KL  PVVLLIS DGFRFGYQFKT TP+I  LIS GTEA+TGLIPVFP++TFPNHYSIATGLYPA HGII N F DPVSG+ F   + +PKWWLGEP+W T VN GL A+TYFWPG++V+KG+WNCP  +C   YN SV  E RVD++L +FD    EIPDFM LYF++PD QGH+ G DDP +T+AVS++D MIGR+I
Sbjct:   47 QPPKKLNKPVVLLISCDGFRFGYQFKTETPNIDLLISRGTEAKTGLIPVFPTMTFPNHYSIATGLYPASHGIIMNKFTDPVSGELFNR-NLNPKWWLGEPLWVTAVNQGLMAATYFWPGADVHKGSWNCPKGFCKAPYNVSVPLEERVDTILNYFDLPEREIPDFMALYFDEPDIQGHEYGPDDPRVTEAVSKVDKMIGRII 247

The following BLAST results are available for this feature:

BLAST of GT624734 vs. TrEMBL
BLAST of GT624734 vs. SwissProt
BLAST of GT624734 vs. TAIR peptide

BLAST of GT624734 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RK58_RICCO	2.314e-96	78.61	Ectonucleotide pyrophosphatase/phosphodiesterase, ...	[more]
B9H8W6_POPTR	3.694e-94	78.61	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SU83_ARATH	7.702e-92	76.12	Nucleotide pyrophosphatase-like protein OS=Arabido...	[more]
D7MCJ0_ARALY	3.236e-90	75.62	Type I phosphodiesterase/nucleotide pyrophosphatas...	[more]
Q9FS13_SPIOL	1.606e-89	73.13	Nucleotide pyrophosphatase-like protein OS=Spinaci...	[more]
B9N5F9_POPTR	1.606e-89	75.12	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q7F5L2_ORYSJ	3.349e-87	71.64	Os01g0196600 protein OS=Oryza sativa subsp. japoni...	[more]
Q42974_ORYSA	3.349e-87	71.64	Nucleotide pyrophosphatase OS=Oryza sativa GN=OSE4...	[more]
B8ADR2_ORYSI	3.349e-87	71.64	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5XLV5_SORBI	2.653e-84	70.65	Putative uncharacterized protein Sb03g002880 OS=So...	[more]

BLAST of GT624734 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ENPP1_HUMAN	8.772e-40	45.73	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP3_HUMAN	7.426e-39	43.72	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP3_BOVIN	9.699e-39	43.72	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP3_PONAB	2.389e-37	42.71	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP1_MOUSE	1.548e-36	42.08	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP1_RAT	3.450e-36	42.08	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP3_RAT	2.236e-35	39.90	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
YGK5_SCHPO	8.497e-35	36.95	Uncharacterized pyrophosphatase/phosphodiesterase ...	[more]
ENPP3_MOUSE	1.602e-33	38.38	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]
ENPP2_RAT	1.404e-29	35.35	Ectonucleotide pyrophosphatase/phosphodiesterase f...	[more]

BLAST of GT624734 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G29680.1	5.264e-94	76.12	\| Symbols: \| Alkaline-phosphatase-like family pro...	[more]
AT4G29700.1	3.662e-79	67.50	\| Symbols: \| Alkaline-phosphatase-like family pro...	[more]
AT4G29690.1	4.049e-78	66.34	\| Symbols: \| Alkaline-phosphatase-like family pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002591	Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase	PFAM	PF01663	Phosphodiest	coord: 42..232 score: 2
IPR017849	Alkaline phosphatase-like, alpha/beta/alpha	GENE3D	3.40.720.10	no description	coord: 33..232 score: 1.1
IPR017850	Alkaline-phosphatase-like, core domain	SUPERFAMILY	53649	Alkaline phosphatase-like	coord: 24..232 score: 3.3
None	No IPR available	PANTHER	PTHR10151	ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE	coord: 63..232 score: 1.8e
None	No IPR available	PANTHER	PTHR10151:SF25	ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE	coord: 63..232 score: 1.8e
None	No IPR available	SEG	seg	seg	coord: 7..31 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT624734 ID=GT624734; Name=GT624734; organism=Lens culinaris; type=EST; length=701bp
CGCCTTTGCATTCCTCTTCCTCTCTTCATCATCTTCTCCTTCTTCTCCTC
CACCACAATCAACTTCAACCACTCACAACAACACTTCTCTTTCTCGTCCT
CTCACCAAACTCAAACACCCTGTAGTCCTTCTAATCTCCTCTGACGGTTT
TCGATTCGGATACCAATTCAAAACCTCTACACCTAGCATAACTCGTTTGA
TTTCAAACGGAACAGAAGCTGAAACAGGCTTAATCCCTGTTTTTCCATCC
TTAACTTTCCCAAATCATTACTCTATCGCTACAGGTCTCTACCCTGCTTA
TCATGGCATCATCAACAACCATTTCATAGATCCGGTTTCCGGTGATCAAT
TCTACATGGGTAGCCATGATCCCAAATGGTGGCTTGGTGAACCCATATGG
AAAACTGTGGTCAACAATGGGTTGAAAGCTTCTACCTATTTCTGGCCTGG
TTCTGAGGTAAACAAAGGTACATGGAACTGTCCTTCTAAATATTGTTTGC
ATTATAATGGTTCTGTTTCTTTTGAGAATAGGGTTGATTCTGTTTTGAAG
TTTTTCGATTTTGCTAGCGACGAAATTCCTGATTTTATGACTCTTTATTT
TGAGGATCCTGATCATCAAGGTCATAAAGTTGGATCTGATGATCCTGAGA
TTACTAAAGCTGTTTCTAGGATTGATAGCATGATAGGTAGGTTGATTACT
G

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002591	Phosphodiest/P_Trfase
IPR017849	Alkaline_Pase-like_a/b/a
IPR017850	Alkaline_phosphatase_core_sf

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