GT625229

EST Overview


Name	GT625229
Unique Name	GT625229
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	727

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT625229 vs. TrEMBL
Match: B7FLK5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 326.25 bits (835), Expect = 1.639e-87
Identity = 174/239 (72.80%), Postives = 181/239 (75.73%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAA 718
            TQSNPENTVGV+TMAGKG+RVL+TPTSDLGKIL CMHGLEIGGE+NLAA IQVAQLALKHRQNKKQQQRI+VF GSPV HEKKMLEMIGRKLKKNSVALDIVNFGE+DEGKTEKLEALL AVNNNDSSHMVHVPPGPNALSDVLISTPIFT                           VDPNLDPELALALRVSM                       GGEQ A SQDATMTE+TST A
Sbjct:   41 TQSNPENTVGVMTMAGKGVRVLSTPTSDLGKILGCMHGLEIGGEINLAAAIQVAQLALKHRQNKKQQQRIVVFCGSPVTHEKKMLEMIGRKLKKNSVALDIVNFGEEDEGKTEKLEALLTAVNNNDSSHMVHVPPGPNALSDVLISTPIFTGDGEGGSGFAAAAAAASAGGVSGYDFGVDPNLDPELALALRVSMEEERARQEAAAKKAAEDASKQEKGGEQQATSQDATMTERTSTEA 279

[Close]

BLAST of GT625229 vs. TrEMBL
Match: C6TGJ3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 5.271e-86
Identity = 173/241 (71.78%), Postives = 181/241 (75.10%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVGVLTMAGKG+RVL TPTSDLGKILACMHGL+IGGE+NLAAGIQVAQLALKHRQNKKQQQRIIVF+GSPVKHEKKMLEMIGRKLKKNSVALDIVNFGE+DEGKTEKLEALLAAVNNND+SH+VHVP GPNALSDVLISTPIFT                           VDPNLDPELALALRVSM                       G EQ A SQ ATMTE+   A SE
Sbjct:   41 TQSNPENTVGVLTMAGKGVRVLVTPTSDLGKILACMHGLDIGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEEDEGKTEKLEALLAAVNNNDTSHIVHVPSGPNALSDVLISTPIFTGDGEGGSGFAAAAATAAAGGVSGFEFGVDPNLDPELALALRVSMEEERARQEAAAKKAAEDAANLEKGDEQQASSQHATMTERAGAATSE 281

[Close]

BLAST of GT625229 vs. TrEMBL
Match: D7SNX7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_23.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018756001 PE=4 SV=1)

HSP 1 Score: 312.383 bits (799), Expect = 2.448e-83
Identity = 166/241 (68.88%), Postives = 181/241 (75.10%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVGVLTMAGKG+RVL TPTSDLGKILACMHGLE+GGE+NLAAGIQVAQLALKHRQNKKQQQRIIVF+GSPVK++KK+LEMIGRKLKKNSVA+DIV+FGEDD+GK EKLEALL +VNNNDSSH+VHVP GPNALSDVLISTPIFT                           VDPNLDPELALALRVSM                        GEQ + SQDATMTE  + AAS+
Sbjct:   41 TQSNPENTVGVLTMAGKGVRVLVTPTSDLGKILACMHGLEVGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGSPVKYDKKVLEMIGRKLKKNSVAIDIVDFGEDDDGKPEKLEALLGSVNNNDSSHIVHVPAGPNALSDVLISTPIFTGDGEGGSGFAAAAAAAAAGGVAGFDFGVDPNLDPELALALRVSMEEERARQEAAAKKAAEEASRQEKEGEQQSSSQDATMTEHANVAASD 281

[Close]

BLAST of GT625229 vs. TrEMBL
Match: B9SXW5_RICCO (26S proteasome non-atpase regulatory subunit, putative OS=Ricinus communis GN=RCOM_0974240 PE=4 SV=1)

HSP 1 Score: 308.531 bits (789), Expect = 3.536e-82
Identity = 164/241 (68.05%), Postives = 180/241 (74.69%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVGVLTMAGKG+RVL TPTSDLGKILACMHGLEIGGE+NLAAGIQVAQLALKHRQNKKQQQRIIVF+GS +KH+KK LEMIG+KLKKN+VALDIV+FGE+D+ K EKLEALLAAVN+ND+SH+VHVPPGP+ALSDVLISTP+FT                           VDPNLDPELALALRVSM                       GGEQ + SQDATMTE  S  ASE
Sbjct:   41 TQSNPENTVGVLTMAGKGVRVLVTPTSDLGKILACMHGLEIGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGSSIKHDKKALEMIGKKLKKNNVALDIVDFGEEDDEKAEKLEALLAAVNSNDTSHIVHVPPGPSALSDVLISTPVFTGDGEGGSGFAAAAAAAAAGGVSGFEFGVDPNLDPELALALRVSMEEERARQEAAAKKAAEEAAKQEKGGEQPSSSQDATMTESASVTASE 281

[Close]

BLAST of GT625229 vs. TrEMBL
Match: O81340_MESCR (26S proteasome regulatory subunit S5A OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 1.755e-81
Identity = 161/241 (66.80%), Postives = 181/241 (75.10%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVG+LTMAGKG+RVL TPTSDLGKILACMHGL++GGE+NLAAGIQ+AQLALKHRQNKKQQQRIIVF+GSPVK++KK+LE IGRKLKKNSVALD+V+FGED++GK+EKLEAL+AAVNNND+SH+VHVP GPNALSDVLISTPIFT                           VDPNLDPELALALRVSM                       GGEQ + SQDATM E +S A  E
Sbjct:   41 TQSNPENTVGILTMAGKGVRVLVTPTSDLGKILACMHGLDMGGEMNLAAGIQIAQLALKHRQNKKQQQRIIVFAGSPVKYDKKVLETIGRKLKKNSVALDVVDFGEDEDGKSEKLEALVAAVNNNDTSHIVHVPAGPNALSDVLISTPIFTGDGEGGSGFAAAAAAAAAGGVSGFDFGVDPNLDPELALALRVSMEEERARQEAAAKRAAEEAGRQEKGGEQQSSSQDATMGESSSAANPE 281

[Close]

BLAST of GT625229 vs. TrEMBL
Match: C6T7C4_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 305.834 bits (782), Expect = 2.292e-81
Identity = 159/195 (81.54%), Postives = 166/195 (85.13%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            TQSNPENTVGVLTMAGKG+RVL TPTSDLGKILACMHGL+IGGE+NLAAGIQVAQLALKHRQNKKQQQRIIVF+GSPVKHEKKMLEMIGRKLKKNSVALDIVNFGE+DEGKTEKLEALLAAVNNND+SH+VHVP GPNALSDVLISTPIFT                           VDPNLDPELALALRVSM
Sbjct:   41 TQSNPENTVGVLTMAGKGVRVLVTPTSDLGKILACMHGLDIGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEEDEGKTEKLEALLAAVNNNDTSHIVHVPSGPNALSDVLISTPIFTGDGEGGSGFAAAAAAAAAGGVSRFEFGVDPNLDPELALALRVSM 235

[Close]

BLAST of GT625229 vs. TrEMBL
Match: B9GYZ3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_553979 PE=4 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 7.372e-80
Identity = 160/241 (66.39%), Postives = 177/241 (73.44%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVG+LTMAGKG+RVL TPTSDLGKILACMHGLEIGGE+NL AGIQVAQLALKHRQNKKQQQRIIVF GSP+KH+KK+LEMIGRKLKKN+VALDIV+FGE+ +GK EKLEALLAAVN+ND+SH+VHVPPGP+ALSDVLI+TPIFT                           VDPNLDPELALALRVSM                         EQ + SQDATMTE  S  A +
Sbjct:   41 TQSNPENTVGILTMAGKGVRVLVTPTSDLGKILACMHGLEIGGEVNLVAGIQVAQLALKHRQNKKQQQRIIVFVGSPIKHDKKVLEMIGRKLKKNNVALDIVDFGEEGDGKAEKLEALLAAVNSNDTSHIVHVPPGPSALSDVLINTPIFTGDGEGGSGFAAAAAAAAADGVSGFEFGVDPNLDPELALALRVSMEEERARQEAAAKTAAEEAAKQEKAREQPSSSQDATMTENNSVPAPD 281

[Close]

BLAST of GT625229 vs. TrEMBL
Match: B9T6I9_RICCO (26S proteasome non-atpase regulatory subunit, putative OS=Ricinus communis GN=RCOM_1131910 PE=4 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.489e-72
Identity = 145/241 (60.17%), Postives = 173/241 (71.78%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAASE 724
            TQSNPENTVG+LTMAGKG+R+L TPT+DLGKILACMH +E+GGE+N+A  IQVAQLALKHRQNK QQQRIIVF+GSPVK++KKMLEMIG+KLKKN+V+LDIVNFGE+++ K EKLEAL++AVNNNDSSH+VH+PPGPNA+SDVLISTP+F                            VDPNLDPELALALRVSM                         E  + SQDATM +  +  A++
Sbjct:   41 TQSNPENTVGILTMAGKGVRILTTPTTDLGKILACMHDVEMGGEINMANAIQVAQLALKHRQNKNQQQRIIVFAGSPVKYDKKMLEMIGKKLKKNNVSLDIVNFGEEEDEKPEKLEALVSAVNNNDSSHIVHIPPGPNAISDVLISTPVFN--GDGGGSGFAAAAAAAAAGGGEFDFGVDPNLDPELALALRVSMEEERARQEAAAKRAAEESSTQGKVDEPSSTSQDATMVDNANDRAAK 279

[Close]

BLAST of GT625229 vs. TrEMBL
Match: C6THY3_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.945e-72
Identity = 137/149 (91.95%), Postives = 145/149 (97.32%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPI 448
            TQSNPENTVGVLTMAGKG+RVL TPTSDLGKILACMHGLEIGGE+NLAAGIQVAQLALKHRQNKKQQQRIIVF+G PV HEKKMLEMIGRKLKKNSVALD+VNFGE+DEGKTEKLEALL+AVNNND+SH+VHVP GPNALSDVLISTPI
Sbjct:   41 TQSNPENTVGVLTMAGKGVRVLVTPTSDLGKILACMHGLEIGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGGPVNHEKKMLEMIGRKLKKNSVALDVVNFGEEDEGKTEKLEALLSAVNNNDTSHIVHVPSGPNALSDVLISTPI 189

[Close]

BLAST of GT625229 vs. TrEMBL
Match: B9HR77_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_804127 PE=4 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 1.820e-70
Identity = 142/240 (59.17%), Postives = 171/240 (71.25%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXGGEQHAISQDATMTEQTSTAAS 721
            TQSNPENTVG+LTMAGK +RVL T TSDLGKIL+CMHGLE+GGE+NL+AGIQVAQLALKHRQNK QQQRIIVF+GSP+K++KKMLE IG+KLKKN+V+LDIV+FGE+++GK EKLEAL AAVN+NDSSH+VH+PPG  A+SD L++TP+FT                           VDPNLDPELALALRVSM                       G E  + SQD TM ++ + A +
Sbjct:   41 TQSNPENTVGILTMAGKQVRVLTTLTSDLGKILSCMHGLEVGGEMNLSAGIQVAQLALKHRQNKNQQQRIIVFAGSPIKYDKKMLETIGKKLKKNNVSLDIVDFGEEEDGKPEKLEALFAAVNSNDSSHIVHIPPGGTAISDALMNTPVFT-GDGEGGSGFAAAAAAAAAGGGDFDFGVDPNLDPELALALRVSMEEERARQEAAAKRAADEAARQEKGEEPSSKSQDTTMVDKAAEATN 279

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_ARATH (26S proteasome non-ATPase regulatory subunit 4 OS=Arabidopsis thaliana GN=MBP1 PE=1 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 1.333e-70
Identity = 137/196 (69.90%), Postives = 157/196 (80.10%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGE-DDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            TQSNPENTVG+LTMAGKG+RVL TPTSDLGKILACMHGL++GGE+NL A IQ+AQLALKHRQNK Q+QRIIVF+GSP+K+EKK LE++G++LKKNSV+LDIVNFGE DDE K +KLEALL AVNNND SH+VHVP G NALSDVL+STP+FT                           VDPN+DPELALALRVSM
Sbjct:   41 TQSNPENTVGILTMAGKGVRVLTTPTSDLGKILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLKKNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNNDGSHIVHVPSGANALSDVLLSTPVFT--GDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELALALRVSM 234

[Close]

BLAST of GT625229 vs. SwissProt
Match: RPN10_YEAST (26S proteasome regulatory subunit RPN10 OS=Saccharomyces cerevisiae GN=RPN10 PE=1 SV=3)

HSP 1 Score: 158.688 bits (400), Expect = 2.997e-38
Identity = 87/195 (44.62%), Postives = 123/195 (63.08%), Query Frame = 2

Query:    8 SNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNN--NDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            SNPENTVG+++ AG   RVL+T T++ GKILA +H  +I G+L++A  +Q+AQL LKHRQNK Q QRI+ F  SP+   +  L  + + LKKN+VA+DI+NFGE ++  TE L+  +AAVNN   ++SH++ V PGP  L + + S+PI                             VDP++DPELA+ALR+SM
Sbjct:   43 SNPENTVGLISGAGANPRVLSTFTAEFGKILAGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAVDIINFGEIEQ-NTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILEEGSSGMGAFGGSGGDSDANGTFMDFGVDPSMDPELAMALRLSM 236

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_DICDI (26S proteasome non-ATPase regulatory subunit 4 OS=Dictyostelium discoideum GN=psmD4 PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.678e-38
Identity = 84/195 (43.08%), Postives = 123/195 (63.08%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            TQSNPE+ V +++MAGK   VL T T +L KIL+    L+I G+++ +  +Q+AQLAL+HRQN  Q  RII F GSP+K  K+ L  + ++LKKN VA+DI+NFGE  E  ++KLEA +  VNNND SH++ VPPGP+ LSD+++ +PI                             ++ + DP+LA+AL++S+
Sbjct:   41 TQSNPESAVSIMSMAGKKPEVLVTLTQELSKILSGAQELKINGKIDFSTTMQIAQLALRHRQNNHQHPRIIAFVGSPLKETKEELIQLAKRLKKNGVAVDIINFGEVTE-NSDKLEAFINDVNNNDESHLLTVPPGPHILSDIILQSPI-----------------VESGSGQFGSEFINADTDPDLAMALKLSL 217

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_MOUSE (26S proteasome non-ATPase regulatory subunit 4 OS=Mus musculus GN=Psmd4 PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 5.291e-35
Identity = 90/197 (45.69%), Postives = 118/197 (59.90%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            T+SNPEN VG++T+A     VL T T D G+IL+ +H ++  G++    GI+VA LALKHRQ K  + RII F GSPV+  +K L  + ++LKK  V +DI+NFGE +E  TEKL A +  +N  D   SH+V VPPGP +L+D LIS+PI                             VDP+ DPELALALRVSM
Sbjct:   41 TRSNPENNVGLITLAND-CEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGE-EEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGP-SLADALISSPILA----------GEGGAMLGLGASDFEFGVDPSADPELALALRVSM 224

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_HUMAN (26S proteasome non-ATPase regulatory subunit 4 OS=Homo sapiens GN=PSMD4 PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 5.291e-35
Identity = 90/197 (45.69%), Postives = 118/197 (59.90%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            T+SNPEN VG++T+A     VL T T D G+IL+ +H ++  G++    GI+VA LALKHRQ K  + RII F GSPV+  +K L  + ++LKK  V +DI+NFGE +E  TEKL A +  +N  D   SH+V VPPGP +L+D LIS+PI                             VDP+ DPELALALRVSM
Sbjct:   41 TRSNPENNVGLITLAND-CEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGE-EEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGP-SLADALISSPILA----------GEGGAMLGLGASDFEFGVDPSADPELALALRVSM 224

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_BOVIN (26S proteasome non-ATPase regulatory subunit 4 OS=Bos taurus GN=PSMD4 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 5.291e-35
Identity = 90/197 (45.69%), Postives = 118/197 (59.90%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            T+SNPEN VG++T+A     VL T T D G+IL+ +H ++  G++    GI+VA LALKHRQ K  + RII F GSPV+  +K L  + ++LKK  V +DI+NFGE +E  TEKL A +  +N  D   SH+V VPPGP +L+D LIS+PI                             VDP+ DPELALALRVSM
Sbjct:   41 TRSNPENNVGLITLAND-CEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGE-EEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGP-SLADALISSPILA----------GEGGAMLGLGASDFEFGVDPSADPELALALRVSM 224

[Close]

BLAST of GT625229 vs. SwissProt
Match: RPN10_SCHPO (26S proteasome regulatory subunit rpn10 OS=Schizosaccharomyces pombe GN=rpn10 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.303e-33
Identity = 79/192 (41.15%), Postives = 111/192 (57.81%), Query Frame = 2

Query:   11 NPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            NPEN  G++T+     +VL+T T D GK L+ MH L + G      GIQ+AQLALKHR+NK Q+QRI+ F GSP+  ++K L  + +++KKN+VA+DI++ GE        L+  + A N++DS H+V +PP P  LSD++  +PI                             VDPNLD ELALAL +SM
Sbjct:   44 NPENMCGLMTIGDNSPQVLSTLTRDYGKFLSAMHDLPVRGNAKFGDGIQIAQLALKHRENKIQRQRIVAFVGSPIVEDEKNLIRLAKRMKKNNVAIDIIHIGELQ--NESALQHFIDAANSSDSCHLVSIPPSPQLLSDLVNQSPI---------------GQGVVASQNQFEYGVDPNLDVELALALELSM 218

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_DROME (26S proteasome non-ATPase regulatory subunit 4 OS=Drosophila melanogaster GN=Pros54 PE=1 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 8.447e-33
Identity = 88/196 (44.90%), Postives = 115/196 (58.67%), Query Frame = 2

Query:    5 QSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            +SNPEN VG++T++   + VLAT TSD G+I + MH ++  GE+NL  GI++A L LKHRQ K  + RI+VF GSP+ HE+  L    ++LKK  V +DIV+FG D     E L A + A+N  D   SH+V VP G + LSD L+S+PI                             VDPN DPELALALRVSM
Sbjct:   42 RSNPENNVGLMTLSNT-VEVLATLTSDAGRIFSKMHLVQPKGEINLLTGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKKEKVNVDIVSFG-DHGNNNEILTAFINALNGKDGTGSHLVSVPRG-SVLSDALLSSPII---------QGEDGMGGAGLGGNVFEFGVDPNEDPELALALRVSM 225

[Close]

BLAST of GT625229 vs. SwissProt
Match: PIPSL_HUMAN (Putative PIP5K1A and PSMD4-like protein OS=Homo sapiens GN=PIPSL PE=5 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 6.054e-31
Identity = 85/197 (43.15%), Postives = 111/197 (56.35%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            T+SNPEN VG++T+      VL T T D G+IL+ +H ++  G++    GI VA LALKHRQ    + RII F G+PV+  +K L  + + LKK  V +DI+NFGE +E  TEKL A +  +N  D   SH+V VPPGP +L+D LIS PI                             VDP+ DPELAL LRV M
Sbjct:  526 TRSNPENNVGLITL-DNDCEVLTTLTPDTGRILSKLHTVQPKGKITFCMGIHVAHLALKHRQGNNHKIRIIAFVGNPVEDNEKNLVKLAKCLKKEKVNVDIINFGE-EEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGP-SLADALISFPILA----------GEGGAMMGLGASDFEFGVDPSADPELALVLRVFM 709

[Close]

BLAST of GT625229 vs. SwissProt
Match: PSMD4_SCHMA (26S proteasome non-ATPase regulatory subunit 4 OS=Schistosoma mansoni PE=2 SV=2)

HSP 1 Score: 111.694 bits (278), Expect = 4.202e-24
Identity = 70/196 (35.71%), Postives = 106/196 (54.08%), Query Frame = 2

Query:    5 QSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGEDDEGKTEKLEALLAAVNNND--SSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            Q NPENT+G+L++A     VL T T+D+ KI   +H +E  G +   + I++A LAL+HRQ + Q+ RI+ F GSP+  ++K L  + ++LKK  V +DI+NFGE +E   +KL   +  +N  D   SH++ V PG   L D L+++P+                             +D   DP+L  ALRVSM
Sbjct:   42 QRNPENTIGLLSLANT--EVLCTLTNDVSKIYNRLHLVEPKGRIIFCSSIRIAHLALRHRQLRHQKMRIVCFIGSPILEDEKELTRLAKRLKKEKVNVDIINFGE-NETNEQKLSEFIDTLNGKDGTGSHLISVAPG-TVLHDTLMTSPVVA----------GEDGSGMAGAGLGLEFGLDGAEDPDLLYALRVSM 223

[Close]

BLAST of GT625229 vs. TAIR peptide
Match: AT4G38630.1 (| Symbols: RPN10, MCB1, ATMCB1, MBP1 | regulatory particle non-ATPase 10 | chr4:18057357-18059459 REVERSE LENGTH=386)

HSP 1 Score: 266.159 bits (679), Expect = 1.331e-71
Identity = 137/196 (69.90%), Postives = 157/196 (80.10%), Query Frame = 2

Query:    2 TQSNPENTVGVLTMAGKGLRVLATPTSDLGKILACMHGLEIGGELNLAAGIQVAQLALKHRQNKKQQQRIIVFSGSPVKHEKKMLEMIGRKLKKNSVALDIVNFGE-DDEGKTEKLEALLAAVNNNDSSHMVHVPPGPNALSDVLISTPIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXVDPNLDPELALALRVSM 586
            TQSNPENTVG+LTMAGKG+RVL TPTSDLGKILACMHGL++GGE+NL A IQ+AQLALKHRQNK Q+QRIIVF+GSP+K+EKK LE++G++LKKNSV+LDIVNFGE DDE K +KLEALL AVNNND SH+VHVP G NALSDVL+STP+FT                           VDPN+DPELALALRVSM
Sbjct:   41 TQSNPENTVGILTMAGKGVRVLTTPTSDLGKILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLKKNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNNDGSHIVHVPSGANALSDVLLSTPVFT--GDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELALALRVSM 234

The following BLAST results are available for this feature:

BLAST of GT625229 vs. TrEMBL
BLAST of GT625229 vs. SwissProt
BLAST of GT625229 vs. TAIR peptide

BLAST of GT625229 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FLK5_MEDTR	1.639e-87	72.80	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TGJ3_SOYBN	5.271e-86	71.78	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7SNX7_VITVI	2.448e-83	68.88	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SXW5_RICCO	3.536e-82	68.05	26S proteasome non-atpase regulatory subunit, puta...	[more]
O81340_MESCR	1.755e-81	66.80	26S proteasome regulatory subunit S5A OS=Mesembrya...	[more]
C6T7C4_SOYBN	2.292e-81	81.54	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
B9GYZ3_POPTR	7.372e-80	66.39	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T6I9_RICCO	1.489e-72	60.17	26S proteasome non-atpase regulatory subunit, puta...	[more]
C6THY3_SOYBN	1.945e-72	91.95	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
B9HR77_POPTR	1.820e-70	59.17	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GT625229 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PSMD4_ARATH	1.333e-70	69.90	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
RPN10_YEAST	2.997e-38	44.62	26S proteasome regulatory subunit RPN10 OS=Sacchar...	[more]
PSMD4_DICDI	6.678e-38	43.08	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
PSMD4_MOUSE	5.291e-35	45.69	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
PSMD4_HUMAN	5.291e-35	45.69	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
PSMD4_BOVIN	5.291e-35	45.69	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
RPN10_SCHPO	1.303e-33	41.15	26S proteasome regulatory subunit rpn10 OS=Schizos...	[more]
PSMD4_DROME	8.447e-33	44.90	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]
PIPSL_HUMAN	6.054e-31	43.15	Putative PIP5K1A and PSMD4-like protein OS=Homo sa...	[more]
PSMD4_SCHMA	4.202e-24	35.71	26S proteasome non-ATPase regulatory subunit 4 OS=...	[more]

BLAST of GT625229 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G38630.1	1.331e-71	69.90	\| Symbols: RPN10, MCB1, ATMCB1, MBP1 \| regulatory ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003903	Ubiquitin interacting motif	PROFILE	PS50330	UIM	coord: 182..201 score: 8
None	No IPR available	PANTHER	PTHR10223	26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4	coord: 1..211 score: 1.2e
None	No IPR available	SEG	seg	seg	coord: 152..178 score: NAcoord: 196..218 score
None	No IPR available	SUPERFAMILY	53300	vWA-like	coord: 1..140 score: 3.5
None	No IPR available	SEG	seg	seg	coord: 25..47 score: NAcoord: 65..91 score
None	No IPR available	SEG	seg	seg	coord: 4..33 score: NAcoord: 63..74 score
None	No IPR available	SEG	seg	seg	coord: 19..40 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT625229 ID=GT625229; Name=GT625229; organism=Lens culinaris; type=EST; length=727bp
AACTCAGTCTAATCCTGAAAATACTGTTGGAGTTCTAACTATGGCGGGGA
AAGGTCTTCGGGTTTTGGCTACTCCTACCAGTGATTTGGGCAAAATCTTA
GCTTGTATGCATGGTCTAGAAATAGGCGGTGAGTTGAACCTAGCTGCTGG
CATTCAGGTGGCACAGTTGGCTCTTAAGCATCGGCAGAATAAGAAGCAGC
AGCAAAGGATTATTGTCTTTTCTGGAAGTCCTGTCAAGCACGAGAAGAAA
ATGTTAGAGATGATTGGGAGAAAGTTGAAAAAGAATAGTGTAGCACTTGA
CATTGTCAACTTTGGTGAAGATGACGAGGGGAAGACAGAGAAGCTTGAAG
CACTCCTTGCAGCGGTAAACAATAATGATAGTAGCCATATGGTTCATGTT
CCACCTGGTCCAAATGCTCTTTCGGACGTACTTATAAGTACACCTATTTT
TACTGGTGACGGAGAAGGTGGAAGTGGTTTTGCAGCAGCTGCAGCAGCAG
CATCTGCTGGTGGCGCATCTGGATATGATTTTGGTGTTGATCCAAACTTG
GATCCTGAATTGGCTCTTGCTCTAAGAGTTTCAATGGAAGAGGAGAGAGC
TAGGCAGGAAGCAGCTGCGAAAAAGGCTGCAGAGGATGCTTCCAAACAGG
AGAAAGGTGGTGAACAGCACGCTATTTCCCAGGATGCAACTATGACTGAG
CAAACCAGTACTGCAGCTTCTGAAGTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR003903	UIM_dom

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