GT620534

EST Overview


Name	GT620534
Unique Name	GT620534
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	728

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT620534 vs. TrEMBL
Match: B9RN21_RICCO (Pectin acetylesterase, putative OS=Ricinus communis GN=RCOM_1342620 PE=4 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 2.616e-85
Identity = 156/232 (67.24%), Postives = 181/232 (78.02%), Query Frame = 3

Query:   63 IRMKLLILFACLLSGFISS-------RSETLFSFSQNDAVSANPLQL---LMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            + M +++L+ C+L G +         +SE +  F ++ A S  P      LMV LTLIQGAD+KGAVCLDGTLP YHL RG  SG NSWLI LEGGGWCNTIR+CVYRK T RGSSK+ME  +PFTGILSNKP+ENPDFFNWNRVKLRYCDGASFSGD  +EAA L FRGQ+IW AA+E LM++GM NA QALLSGCSAGGLASI+HCDEF+ L P+S+KVKC SDAG FLD
Sbjct:    7 MEMGIMLLYGCILIGMVLGGSVDVILKSEVMLPFLESGASSPAPPAAPKPLMVGLTLIQGADAKGAVCLDGTLPAYHLHRGSGSGQNSWLIQLEGGGWCNTIRSCVYRKTTRRGSSKFMEKVLPFTGILSNKPDENPDFFNWNRVKLRYCDGASFSGDYHDEAAQLYFRGQRIWSAAMENLMAEGMLNATQALLSGCSAGGLASILHCDEFRDLFPQSTKVKCLSDAGLFLD 238

[Close]

BLAST of GT620534 vs. TrEMBL
Match: B9RFS1_RICCO (Pectin acetylesterase, putative OS=Ricinus communis GN=RCOM_1436870 PE=4 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 4.933e-84
Identity = 145/182 (79.67%), Postives = 164/182 (90.11%), Query Frame = 3

Query:  183 LMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            LMV LTLI+ A ++GAVCLDGTLPGYHL RG+ SGANSWLI LEGGGWCN IRNCVYRKKT RGSSKYME Q+ FTGILSNKP+ENPDFFNWNRVKLRYCDGASFSGD+EN+AA LQFRGQ+IWLAA+++LMS+GM  A QALLSGCSAGGLASI+HCDEF++L P+ ++VKC SDAG FLD
Sbjct:   53 LMVGLTLIRSAGARGAVCLDGTLPGYHLHRGYGSGANSWLIQLEGGGWCNNIRNCVYRKKTRRGSSKYMEKQLAFTGILSNKPQENPDFFNWNRVKLRYCDGASFSGDNENKAAQLQFRGQRIWLAAMQDLMSKGMRYANQALLSGCSAGGLASILHCDEFRNLFPRRTRVKCLSDAGLFLD 234

[Close]

BLAST of GT620534 vs. TrEMBL
Match: A5C8P7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020113 PE=4 SV=1)

HSP 1 Score: 311.997 bits (798), Expect = 3.198e-83
Identity = 150/200 (75.00%), Postives = 168/200 (84.00%), Query Frame = 3

Query:  129 TLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            T  SFS +  VSA   + +MV LTLI  A +KGAVCLDGTLPGYHL RG+ SGANSWLI LEGGGWCN+IR CVYRKKT RGSS YME QIPFTGILSN PEENPDFFNWNRVKLRYCDGASF+GDS+N+AA L FRGQ+IW AAIE+LMS+GM  A QALLSGCSAGGLA+I+HCDEF+   P+++KVKC SDAG FLD
Sbjct:   41 TELSFSDSYGVSAAS-RPMMVGLTLIHAAAAKGAVCLDGTLPGYHLHRGYGSGANSWLIQLEGGGWCNSIRTCVYRKKTRRGSSIYMEKQIPFTGILSNNPEENPDFFNWNRVKLRYCDGASFTGDSQNQAAQLNFRGQRIWSAAIEDLMSKGMRYANQALLSGCSAGGLAAILHCDEFRGFFPRNTKVKCLSDAGLFLD 239

[Close]

BLAST of GT620534 vs. TrEMBL
Match: B9I994_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_572510 PE=4 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 4.176e-83
Identity = 155/238 (65.13%), Postives = 182/238 (76.47%), Query Frame = 3

Query:   66 RMKLLILFACLLSGFISSRSETLF-----------------SFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            ++++L LF C L G + S S  +F                 + S + +V+  PL   MV LTLIQGADS GAVCLDGTLPGYHLDRG  +G +SWL+ LEGGGWC+TIRNCVYRK T RGSSK  E Q+PFTGILS+K EENPDFFNWNRVK+RYCDGASFSGDS+NEA+ L FRGQ+IW AA+E LM++GM NA QALLSGCSAGGLASIIHCDEF+ L P+S+KVKC SDAG FL+
Sbjct:    3 QVQVLSLFVCTLIGNVLSGSNHVFQDLKDREGVLSFLEYYAAVSPSSSVTDKPL---MVGLTLIQGADSSGAVCLDGTLPGYHLDRGSGTGKDSWLVQLEGGGWCDTIRNCVYRKTTRRGSSKLFEKQLPFTGILSDKAEENPDFFNWNRVKVRYCDGASFSGDSQNEASQLYFRGQRIWSAAMEYLMAEGMQNATQALLSGCSAGGLASIIHCDEFRELFPQSTKVKCLSDAGMFLN 237

[Close]

BLAST of GT620534 vs. TrEMBL
Match: D7UAB5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031342001 PE=4 SV=1)

HSP 1 Score: 307.76 bits (787), Expect = 6.031e-82
Identity = 143/181 (79.01%), Postives = 158/181 (87.29%), Query Frame = 3

Query:  186 MVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            MV LTLI  A +KGAVCLDGTLPGYHL RG+ SGANSWLI LEGGGWCN+IR CVYRKKT RGSS YME QIPFTGILSN PEENPDFFNWNRVKLRYCDGASF+GDS+N+AA L FRGQ+IW AAIE+LMS+GM  A QALLSGCSAGGLA+I+HCDEF+   P+++KVKC SDAG FLD
Sbjct:    1 MVGLTLIHAAAAKGAVCLDGTLPGYHLHRGYGSGANSWLIQLEGGGWCNSIRTCVYRKKTRRGSSIYMEKQIPFTGILSNNPEENPDFFNWNRVKLRYCDGASFTGDSQNQAAQLNFRGQRIWSAAIEDLMSKGMRYANQALLSGCSAGGLAAILHCDEFRGFFPRNTKVKCLSDAGLFLD 181

[Close]

BLAST of GT620534 vs. TrEMBL
Match: B9I6B6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_240173 PE=4 SV=1)

HSP 1 Score: 306.99 bits (785), Expect = 1.029e-81
Identity = 142/182 (78.02%), Postives = 160/182 (87.91%), Query Frame = 3

Query:  183 LMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            L+V LTLI+ A +KGAVCLDGTLPGYH  RG+ SGANSWLI LEGGGWCNT+R CVYRKKT RGSS YME Q+ FTGILSNKPEENPDFFNWNRVKLRYCDGASF+GDSE++AA LQFRGQ+IW AA+E+LMS GM  A QALLSGCSAGGLASI+HCDEF+ L P++++VKC SDAG FLD
Sbjct:    3 LLVGLTLIKSAAAKGAVCLDGTLPGYHWHRGYGSGANSWLIQLEGGGWCNTVRACVYRKKTRRGSSNYMEKQVAFTGILSNKPEENPDFFNWNRVKLRYCDGASFTGDSEHKAAQLQFRGQRIWSAAMEDLMSSGMRYANQALLSGCSAGGLASILHCDEFRDLFPRTTRVKCLSDAGLFLD 184

[Close]

BLAST of GT620534 vs. TrEMBL
Match: D7LS88_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_907665 PE=4 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 8.708e-81
Identity = 140/181 (77.35%), Postives = 158/181 (87.29%), Query Frame = 3

Query:  186 MVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            MV LTLI+GADSKGAVCLDGTLPGYHLDRGF SGANSW IHLEGGGWCN  R+CVYRK + RGSSK+ME  +PFTGILSNKPEENPDFFNWNR+KLRYCDGASFSGDS++E++ + +RGQ+IW  A+EE +S GM  A QALLSGCSAGGLASI+HCDE++ LLP S KVKC SDAG FLD
Sbjct:    7 MVPLTLIEGADSKGAVCLDGTLPGYHLDRGFGSGANSWPIHLEGGGWCNNHRSCVYRKTSPRGSSKFMEKALPFTGILSNKPEENPDFFNWNRIKLRYCDGASFSGDSQDESSQIFYRGQRIWQVAMEEFLSLGMKQANQALLSGCSAGGLASILHCDEYRELLPSSRKVKCLSDAGMFLD 187

[Close]

BLAST of GT620534 vs. TrEMBL
Match: Q2HRU2_MEDTR (Pectinacetylesterase OS=Medicago truncatula GN=MtrDRAFT_AC157894g3v2 PE=4 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 1.137e-80
Identity = 137/182 (75.27%), Postives = 160/182 (87.91%), Query Frame = 3

Query:  183 LMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            L+V LTL+  A +KGAVCLDGTLPGYHL RG+ SGA+SWL++LEGGGWCN +R+CVYRKKT RGSS YME +IPFTGILSNKPEENPDFFNWNR KLRYCDG SF+GD E++ A LQFRGQ+IW AA+E+L+S+GMH A QALLSGCSAGGLA+IIHCDEF+ L P+++KVKC SDAG FLD
Sbjct:   54 LLVGLTLVHNAAAKGAVCLDGTLPGYHLHRGYGSGADSWLVNLEGGGWCNNVRSCVYRKKTRRGSSLYMEKEIPFTGILSNKPEENPDFFNWNRAKLRYCDGGSFAGDGEDQDAELQFRGQRIWAAAMEDLISKGMHFANQALLSGCSAGGLATIIHCDEFRGLFPRTTKVKCLSDAGLFLD 235

[Close]

BLAST of GT620534 vs. TrEMBL
Match: D7T6B8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017717001 PE=4 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.485e-80
Identity = 140/181 (77.35%), Postives = 159/181 (87.85%), Query Frame = 3

Query:  186 MVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            MV L+LI GA S GAVCLDGTLPGYHL RG+ SGANSWLIHLEGGGWCNTIR CV+RKKT  GSS +ME  IPFTGILSN+ EENPDF+NWNRVKLRYCDGASFSGDS+NEA  L FRGQ+IWLAA+++LMS+GMH A QALLSGCSAGGLA+I+HCDEF+ L P++++VKC SDAG FLD
Sbjct:    1 MVGLSLIAGAASTGAVCLDGTLPGYHLHRGYGSGANSWLIHLEGGGWCNTIRTCVFRKKTPHGSSTHMEKLIPFTGILSNRAEENPDFYNWNRVKLRYCDGASFSGDSQNEAKTLYFRGQRIWLAAMKDLMSKGMHYANQALLSGCSAGGLAAILHCDEFRELFPRTTRVKCLSDAGLFLD 181

[Close]

BLAST of GT620534 vs. TrEMBL
Match: D7LSL8_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_665764 PE=4 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 3.309e-80
Identity = 143/202 (70.79%), Postives = 167/202 (82.67%), Query Frame = 3

Query:  123 SETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            +ET  SF +   ++ +   + MV LTLI+GADSKGAVCLDGTLPGYHLDRGF SGANSW IHLEGGGWCN  R+CVYRK + RGSSK+ME  +PFTGILSNKPEENPDFF+WNR+KLRYCDGASFSGDS++E++ + +RGQ+IW  A+EE +S GM  A QALLSGCSAGGLASI+HCDE+  LLP S KVKC SDAG FLD
Sbjct:   10 TETAISFLEYKLMAPS---VPMVPLTLIEGADSKGAVCLDGTLPGYHLDRGFGSGANSWPIHLEGGGWCNNHRSCVYRKTSPRGSSKFMEKALPFTGILSNKPEENPDFFHWNRIKLRYCDGASFSGDSQDESSQIFYRGQRIWQMAMEEFLSLGMKQANQALLSGCSAGGLASILHCDEYMELLPSSRKVKCLSDAGMFLD 208

[Close]

BLAST of GT620534 vs. SwissProt
Match: NOTUM_MOUSE (Protein notum homolog OS=Mus musculus GN=Notum PE=2 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 1.265e-20
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 3

Query:  234 CLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRG--SSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDS-ENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPK----SSKVKCFSDAGFFLD 728
            C DG+  GY+L      G+  WL+ LEGG +C    NC  R  T R   SSK   +    TGILS++PEENP ++N N V + YC    +SG S +++     F G  I    + EL+ +G+  A+  LL+G SAGG   +++ D    LL +    S +V+  +D+G+FLD
Sbjct:  108 CNDGSPAGYYLKES--KGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLD 277

[Close]

BLAST of GT620534 vs. SwissProt
Match: NOTUM_HUMAN (Protein notum homolog OS=Homo sapiens GN=NOTUM PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.399e-19
Identity = 63/172 (36.63%), Postives = 92/172 (53.49%), Query Frame = 3

Query:  234 CLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRG--SSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSG-DSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPK----SSKVKCFSDAGFFLD 728
            C DG+  GY+L      G+  WL+ LEGG +C    NC  R  T R   SS+        TGILS++PEENP ++N N V + YC    +SG  S++E     F G  I    + EL+ +G+  A+  LL+G SAGG   +++ D     L K    + +V+  +D+G+FLD
Sbjct:  101 CNDGSPAGYYLKES--RGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLD 270

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT3G62060.1 (| Symbols: | Pectinacetylesterase family protein | chr3:22980123-22982734 FORWARD LENGTH=419)

HSP 1 Score: 299.286 bits (765), Expect = 1.419e-81
Identity = 142/202 (70.30%), Postives = 166/202 (82.18%), Query Frame = 3

Query:  123 SETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            +ET  SF +   ++ +   + M+ LTLI GADSKGAVCLDGTLPGYHLDRGF SGANSWLI LEGGGWCN  R+CVYRK + RGSSK+ME  + FTGILSN+ EENPDFFNWNR+KLRYCDGASFSGDS++E++ L +RGQ+IW  A+EE +S GM  A QALLSGCSAGGLASI+HCDEF+ LLP S+KVKC SDAG FLD
Sbjct:   36 TETAISFLEYKLMAPS---VPMIPLTLIHGADSKGAVCLDGTLPGYHLDRGFGSGANSWLIQLEGGGWCNNHRSCVYRKTSRRGSSKFMEKALAFTGILSNRSEENPDFFNWNRIKLRYCDGASFSGDSQDESSQLFYRGQRIWQVAMEEFLSLGMKQANQALLSGCSAGGLASILHCDEFRELLPSSTKVKCLSDAGMFLD 234

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT1G57590.1 (| Symbols: | Pectinacetylesterase family protein | chr1:21327458-21329707 REVERSE LENGTH=444)

HSP 1 Score: 298.13 bits (762), Expect = 3.162e-81
Identity = 140/195 (71.79%), Postives = 164/195 (84.10%), Query Frame = 3

Query:  144 SQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            +QND  S    +  MV LTLIQ A +KGAVCLDG+LPGYHL RGF SGAN+WL+ LEGGGWC+TIRNCVYRK T RGSS YME +IPFTGILS+K  +NPDF+NWNRVK+RYCDG SFSGDSEN+AA LQFRG++IWLAA+E+LM++GM  A+QALLSGCSAGGLA I+ CD+F  L P S++VKC SDAGFFLD
Sbjct:   67 TQNDVFSE--AKFPMVGLTLIQSAAAKGAVCLDGSLPGYHLHRGFGSGANNWLVQLEGGGWCDTIRNCVYRKTTRRGSSSYMEKEIPFTGILSDKAADNPDFYNWNRVKVRYCDGGSFSGDSENKAAQLQFRGKRIWLAAMEDLMAKGMRQAKQALLSGCSAGGLAVILRCDDFGKLFPPSTRVKCLSDAGFFLD 259

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT5G26670.1 (| Symbols: | Pectinacetylesterase family protein | chr5:9318456-9320816 FORWARD LENGTH=416)

HSP 1 Score: 295.819 bits (756), Expect = 1.569e-80
Identity = 144/198 (72.73%), Postives = 165/198 (83.33%), Query Frame = 3

Query:  135 FSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            F FS+  + + NPL   MV LTLI+GA SKGAVCLDGTLPGYHL RG  SGANSWLI LEGGGWC+ IRNCVYRKK+ RGSS YME QI FTGILSNK +ENPDFFNWNRVKLRYCDG SFSGDS+N+AA LQFRG+KIW AA+++L ++GM NA+QALLSGCSAGGLA I+ CDEF++L    ++VKC SDAG FLD
Sbjct:   39 FGFSKFSS-NFNPL---MVGLTLIRGAGSKGAVCLDGTLPGYHLHRGHGSGANSWLIQLEGGGWCDNIRNCVYRKKSRRGSSNYMEKQIQFTGILSNKAQENPDFFNWNRVKLRYCDGGSFSGDSQNKAARLQFRGEKIWRAAMDDLKAKGMRNAKQALLSGCSAGGLAVILRCDEFRNLFSGWTRVKCLSDAGLFLD 232

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT3G05910.2 (| Symbols: | Pectinacetylesterase family protein | chr3:1764389-1767240 REVERSE LENGTH=311)

HSP 1 Score: 293.893 bits (751), Expect = 5.963e-80
Identity = 145/201 (72.14%), Postives = 164/201 (81.59%), Query Frame = 3

Query:  126 ETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            E  F FS+  + + NPL   MV LTLI+GADS GAVCLDGTLPGYHL RG  SGANSWLI LEGGGWCN IR CVYRK T RGSS YME Q+ FTGILS+K +ENPDFFNWNRVKLRYCDGASFSGD +N+AA LQFRG++IW AAI++L + GM  A QALLSGCSAGGLA+I+ CDEF++L P S+KVKC SDAG FLD
Sbjct:   36 ELEFGFSKYSS-NLNPL---MVGLTLIRGADS-GAVCLDGTLPGYHLHRGHGSGANSWLIQLEGGGWCNNIRTCVYRKTTRRGSSNYMEKQLQFTGILSDKAQENPDFFNWNRVKLRYCDGASFSGDGQNQAAQLQFRGERIWRAAIDDLKANGMRYANQALLSGCSAGGLAAILRCDEFRNLFPGSTKVKCLSDAGLFLD 231

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT3G05910.1 (| Symbols: | Pectinacetylesterase family protein | chr3:1764509-1767240 REVERSE LENGTH=415)

HSP 1 Score: 293.893 bits (751), Expect = 5.963e-80
Identity = 145/201 (72.14%), Postives = 164/201 (81.59%), Query Frame = 3

Query:  126 ETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            E  F FS+  + + NPL   MV LTLI+GADS GAVCLDGTLPGYHL RG  SGANSWLI LEGGGWCN IR CVYRK T RGSS YME Q+ FTGILS+K +ENPDFFNWNRVKLRYCDGASFSGD +N+AA LQFRG++IW AAI++L + GM  A QALLSGCSAGGLA+I+ CDEF++L P S+KVKC SDAG FLD
Sbjct:   36 ELEFGFSKYSS-NLNPL---MVGLTLIRGADS-GAVCLDGTLPGYHLHRGHGSGANSWLIQLEGGGWCNNIRTCVYRKTTRRGSSNYMEKQLQFTGILSDKAQENPDFFNWNRVKLRYCDGASFSGDGQNQAAQLQFRGERIWRAAIDDLKANGMRYANQALLSGCSAGGLAAILRCDEFRNLFPGSTKVKCLSDAGLFLD 231

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT2G46930.1 (| Symbols: | Pectinacetylesterase family protein | chr2:19283625-19286090 FORWARD LENGTH=416)

HSP 1 Score: 291.967 bits (746), Expect = 2.266e-79
Identity = 140/218 (64.22%), Postives = 170/218 (77.98%), Query Frame = 3

Query:   75 LLILFACLLSGFISSRSETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            L I+   + SG +    + + S  ++  V  +P QLLMV LTLIQ A SKGAVCLDGTLPGYHL  G  SGAN WLI LEGGGWCNT R+C++RK T RGSS +ME  + FTGILSNK  ENPDFFNWNRVKLRYCDGASF+GDS++E++ L +RGQ+IW +A+EEL+S+GM  AEQALLSGCSAGGLASI+HCD+F+ L P ++ VKC SDAG F+D
Sbjct:   16 LFIVLGVIGSGNVRDTDDEI-SLLESQLVVTSPSQLLMVPLTLIQAAASKGAVCLDGTLPGYHLHPGSGSGANRWLIQLEGGGWCNTRRSCIFRKTTRRGSSNHMEKVLAFTGILSNKSNENPDFFNWNRVKLRYCDGASFTGDSQDESSQLYYRGQRIWHSAMEELLSKGMQKAEQALLSGCSAGGLASILHCDQFKELFPGTTTVKCLSDAGMFMD 232

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BLAST of GT620534 vs. TAIR peptide
Match: AT1G09550.1 (| Symbols: | Pectinacetylesterase family protein | chr1:3089733-3092254 REVERSE LENGTH=388)

HSP 1 Score: 265.003 bits (676), Expect = 2.966e-71
Identity = 125/217 (57.60%), Postives = 169/217 (77.88%), Query Frame = 3

Query:   78 LILFACLLSGFISSRSETLFSFSQNDAVSANPLQLLMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSENEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            LILF+ L  G + +       F++++  +AN   +LMV LTL+Q A +KGAVCLDG++PGYHL RG+ SGAN+W+I L+GG WC++I+NC  RK +  GSS  ME ++ F G+LSNK  ENPDF+NWN+VK+RYCDGASF GDSEN+AA LQ+RG++I+LA +E+LM +GM  A+QALLSGCS+GGL++I+ CD+F +L P ++ VKC SDAGFFLD
Sbjct:   15 LILFSILAHGEMKT-------FNESNGTNAN---VLMVGLTLVQAAAAKGAVCLDGSVPGYHLCRGYGSGANNWIIQLQGGAWCDSIQNCQSRKGSGYGSSTLMEKELAFLGLLSNKAAENPDFYNWNKVKVRYCDGASFDGDSENKAAQLQYRGKRIFLAVMEDLMEKGMRQAKQALLSGCSSGGLSAILRCDDFNNLFPPTTTVKCMSDAGFFLD 221

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BLAST of GT620534 vs. TAIR peptide
Match: AT4G19420.1 (| Symbols: | Pectinacetylesterase family protein | chr4:10587504-10589807 REVERSE LENGTH=397)

HSP 1 Score: 254.218 bits (648), Expect = 5.236e-68
Identity = 117/184 (63.59%), Postives = 144/184 (78.26%), Query Frame = 3

Query:  183 LMVNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSE--NEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            L VN+T ++ A +KGAVCLDG+ P YHLDRG  +G NSWLI LEGGGWCN + NCV R  T  GSSK M   + F+ ILSNK + NPDF+NWNRVK+RYCDGASF+GD E  N A  L FRG ++WLA ++EL+++GM NAE A+LSGCSAGGLAS++HCD F++LLP  +KVKC SDAGFFL+
Sbjct:   23 LFVNITFVRNAVAKGAVCLDGSPPAYHLDRGSGTGINSWLIQLEGGGWCNNVTNCVSRMHTRLGSSKKMVENLAFSAILSNKKQYNPDFYNWNRVKVRYCDGASFTGDVEAVNPATNLHFRGARVWLAVMQELLAKGMINAENAVLSGCSAGGLASLMHCDSFRALLPMGTKVKCLSDAGFFLN 206

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BLAST of GT620534 vs. TAIR peptide
Match: AT5G23870.1 (| Symbols: | Pectinacetylesterase family protein | chr5:8046309-8049999 REVERSE LENGTH=415)

HSP 1 Score: 244.202 bits (622), Expect = 5.418e-65
Identity = 108/182 (59.34%), Postives = 143/182 (78.57%), Query Frame = 3

Query:  189 VNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSE--NEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            V++TL++ A + GA CLDG+LP YHLDRGF +G+N+W++  EGGGWCN I +CV R KT RGS++YM   + FTG+LSN   +NPDF+NWN+V+LRYCDGASF+GDS+  N  + L FRGQ+IW A I +L+ +G+  A +ALL+GCSAGGL++ +HCD F S LPK++ VKC SDAGFFLD
Sbjct:   35 VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTRRGSTRYMSKTVVFTGVLSNNASQNPDFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFRGQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGFFLD 216

[Close]

BLAST of GT620534 vs. TAIR peptide
Match: AT5G23870.3 (| Symbols: | Pectinacetylesterase family protein | chr5:8046060-8049999 REVERSE LENGTH=451)

HSP 1 Score: 244.202 bits (622), Expect = 5.418e-65
Identity = 108/182 (59.34%), Postives = 143/182 (78.57%), Query Frame = 3

Query:  189 VNLTLIQGADSKGAVCLDGTLPGYHLDRGFESGANSWLIHLEGGGWCNTIRNCVYRKKTHRGSSKYMENQIPFTGILSNKPEENPDFFNWNRVKLRYCDGASFSGDSE--NEAAGLQFRGQKIWLAAIEELMSQGMHNAEQALLSGCSAGGLASIIHCDEFQSLLPKSSKVKCFSDAGFFLD 728
            V++TL++ A + GA CLDG+LP YHLDRGF +G+N+W++  EGGGWCN I +CV R KT RGS++YM   + FTG+LSN   +NPDF+NWN+V+LRYCDGASF+GDS+  N  + L FRGQ+IW A I +L+ +G+  A +ALL+GCSAGGL++ +HCD F S LPK++ VKC SDAGFFLD
Sbjct:   35 VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTRRGSTRYMSKTVVFTGVLSNNASQNPDFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFRGQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGFFLD 216

The following BLAST results are available for this feature:

BLAST of GT620534 vs. TrEMBL
BLAST of GT620534 vs. SwissProt
BLAST of GT620534 vs. TAIR peptide

BLAST of GT620534 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RN21_RICCO	2.616e-85	67.24	Pectin acetylesterase, putative OS=Ricinus communi...	[more]
B9RFS1_RICCO	4.933e-84	79.67	Pectin acetylesterase, putative OS=Ricinus communi...	[more]
A5C8P7_VITVI	3.198e-83	75.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9I994_POPTR	4.176e-83	65.13	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7UAB5_VITVI	6.031e-82	79.01	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9I6B6_POPTR	1.029e-81	78.02	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7LS88_ARALY	8.708e-81	77.35	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q2HRU2_MEDTR	1.137e-80	75.27	Pectinacetylesterase OS=Medicago truncatula GN=Mtr...	[more]
D7T6B8_VITVI	1.485e-80	77.35	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LSL8_ARALY	3.309e-80	70.79	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]

BLAST of GT620534 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
NOTUM_MOUSE	1.265e-20	37.21	Protein notum homolog OS=Mus musculus GN=Notum PE=...	[more]
NOTUM_HUMAN	1.399e-19	36.63	Protein notum homolog OS=Homo sapiens GN=NOTUM PE=...	[more]

BLAST of GT620534 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G62060.1	1.419e-81	70.30	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT1G57590.1	3.162e-81	71.79	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT5G26670.1	1.569e-80	72.73	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT3G05910.2	5.963e-80	72.14	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT3G05910.1	5.963e-80	72.14	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT2G46930.1	2.266e-79	64.22	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT1G09550.1	2.966e-71	57.60	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT4G19420.1	5.236e-68	63.59	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT5G23870.1	5.418e-65	59.34	\| Symbols: \| Pectinacetylesterase family protein ...	[more]
AT5G23870.3	5.418e-65	59.34	\| Symbols: \| Pectinacetylesterase family protein ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004963	Pectinacetylesterase	PANTHER	PTHR21562:SF1	PECTIN ACETYLESTERASE	coord: 14..231 score: 1e
None	No IPR available	PANTHER	PTHR21562	NOTUM-RELATED	coord: 14..231 score: 1e
None	No IPR available	PFAM	PF03283	PAE	coord: 49..231 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620534 ID=GT620534; Name=GT620534; organism=Lens culinaris; type=EST; length=728bp
CAGAAACTGAGAGTGTGAAGAACCTTAGTAGTTAGTGGCAATCAATCAGT
ATTAGAGTAGACATTAGAATGAAGCTGCTGATTTTGTTTGCATGCTTACT
CTCTGGGTTTATCTCCTCTAGATCTGAGACACTGTTTTCTTTCTCACAAA
ACGATGCCGTTTCAGCTAATCCATTGCAACTTCTTATGGTTAACCTCACT
CTCATTCAAGGAGCTGATTCTAAAGGAGCTGTTTGTTTGGATGGAACACT
GCCTGGTTACCATTTGGATCGCGGATTTGAATCTGGTGCAAATAGTTGGC
TTATTCATCTCGAGGGAGGAGGATGGTGTAACACTATCAGAAACTGTGTG
TATAGAAAAAAGACTCATCGTGGTTCCTCTAAATACATGGAAAATCAAAT
ACCATTCACAGGAATATTGAGTAATAAACCAGAAGAAAACCCTGATTTCT
TTAACTGGAATAGAGTCAAATTAAGGTACTGTGATGGAGCATCTTTTAGT
GGAGATAGTGAAAATGAGGCTGCAGGGCTTCAATTTCGAGGACAAAAAAT
ATGGCTAGCTGCAATAGAGGAATTAATGTCTCAAGGAATGCATAATGCCG
AACAGGCCCTTCTCTCTGGATGTTCTGCGGGTGGCCTTGCATCCATAATA
CATTGTGATGAGTTCCAGAGCTTGTTACCAAAATCTTCCAAAGTGAAGTG
TTTTAGTGATGCAGGATTTTTTCTTGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR004963	PAE/NOTUM

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