FG531214

EST Overview
NameFG531214
Unique NameFG531214
TypeEST
OrganismPisum sativum (pea)
Sequence length405
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Libraries
Library NameType
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTscdna_library
Homology
[Close]
BLAST of FG531214 vs. TrEMBL
Match: Q1KT07_MEDTR (3-hydroxy-3-methylglutaryl CoA synthase OS=Medicago truncatula GN=HMGS PE=2 SV=1)

HSP 1 Score: 280.026 bits (715), Expect = 5.271e-74
Identity = 132/134 (98.51%), Postives = 134/134 (100.00%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTC+KNF
Sbjct:   95 SIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCFKNF 228
[Close]
BLAST of FG531214 vs. TrEMBL
Match: Q6QLW8_HEVBR (HMG-CoA synthase 2 OS=Hevea brasiliensis GN=HMGS2 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 2.292e-69
Identity = 122/134 (91.04%), Postives = 131/134 (97.76%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDS NACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGP+API+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKFGNTDIEGVDSANACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPEAPIAFESKFRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: A9ZMZ7_HEVBR (Hydroxymethylglutaryl-CoA synthase OS=Hevea brasiliensis GN=HbHMGS2 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 2.292e-69
Identity = 122/134 (91.04%), Postives = 132/134 (98.51%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGP+API+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKFGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPEAPIAFESKFRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: Q94ET0_HEVBR (Hydroxymethylglutaryl coenzyme A synthase OS=Hevea brasiliensis PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.993e-69
Identity = 121/134 (90.30%), Postives = 132/134 (98.51%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIA+L+GPDAPI+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKFGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAILVGPDAPIAFESKFRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: Q944F8_HEVBR (Hydroxymethylglutaryl coenzyme A synthase OS=Hevea brasiliensis PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.993e-69
Identity = 121/134 (90.30%), Postives = 132/134 (98.51%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIA+L+GPDAPI+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKFGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAILVGPDAPIAFESKFRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: B9GKQ4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_548531 PE=4 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.993e-69
Identity = 124/134 (92.54%), Postives = 130/134 (97.01%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPI FESK RGSHM+HVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK F
Sbjct:   91 SIKTFLMQIFEKCGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKFRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKTF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: A9ZMZ6_HEVBR (Hydroxymethylglutaryl-CoA synthase OS=Hevea brasiliensis GN=HbHMGS1 PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.993e-69
Identity = 121/134 (90.30%), Postives = 132/134 (98.51%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIA+L+GPDAPI+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKFGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAILVGPDAPIAFESKFRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: A9PBS1_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.993e-69
Identity = 124/134 (92.54%), Postives = 130/134 (97.01%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK GNTDIEGVDSTNACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPI FESK RGSHM+HVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK F
Sbjct:   91 SIKTFLMQIFEKCGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKFRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKTF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: B9RC08_RICCO (Hydroxymethylglutaryl-CoA synthase, putative OS=Ricinus communis GN=RCOM_1683050 PE=4 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 3.909e-69
Identity = 122/134 (91.04%), Postives = 132/134 (98.51%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK+GNTDIEGVDS+NACYGGTAALFNCVNWVES+SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPI+FESK RGSHM+H YDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+F
Sbjct:   91 SIKTFLMQIFEKHGNTDIEGVDSSNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESKYRGSHMSHAYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHF 224
[Close]
BLAST of FG531214 vs. TrEMBL
Match: B9GW51_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_646543 PE=4 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 5.106e-69
Identity = 123/134 (91.79%), Postives = 130/134 (97.01%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +IK+FLMQIFEK GNTDIEGVDSTNACYGGTAALFNCVNWVES SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAML+GPDAPI+FESK RGSHM+HVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK F
Sbjct:   91 SIKTFLMQIFEKCGNTDIEGVDSTNACYGGTAALFNCVNWVESCSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPITFESKFRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQF 224
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS_ARATH (Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana GN=HMGS PE=2 SV=2)

HSP 1 Score: 260.766 bits (665), Expect = 1.628e-69
Identity = 121/133 (90.98%), Postives = 129/133 (96.99%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKN 401
            +IK+FLMQ+FEK GNTD+EGVDSTNACYGGTAAL NCVNWVESNSWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAMLIGPDAPI FESKLR SHMAHVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+
Sbjct:   91 SIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKH 223
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_CHICK (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Gallus gallus GN=HMGCS1 PE=1 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 9.586e-54
Identity = 93/131 (70.99%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++K+ LMQ+FE+ GNTD+EG+D+TNACYGGTAALFN +NW+ES+SWDGRY LVV  D AVYA G ARPTGGA A+AML+G +AP+ FE  LRG+HM H YDFYKPD+ SEYPVVDGKLS  CYL ALD CY
Sbjct:  103 SVKTVLMQLFEESGNTDVEGIDTTNACYGGTAALFNAINWIESSSWDGRYALVVAGDIAVYATGNARPTGGAGAVAMLVGSNAPLIFERGLRGTHMQHAYDFYKPDMVSEYPVVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_RAT (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Rattus norvegicus GN=Hmgcs1 PE=1 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 1.252e-53
Identity = 93/131 (70.99%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++KS LMQ+FE+ GNTDIEG+D+TNACYGGTAA+FN VNW+ES+SWDGRY LVV  D A+YA G ARPTGG  A+A+LIGP+AP+ F+  LRG+HM H YDFYKPD+ SEYPVVDGKLS  CYL ALD CY
Sbjct:  103 SVKSNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAIYASGNARPTGGVGAVALLIGPNAPVIFDRGLRGTHMQHAYDFYKPDMLSEYPVVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_MOUSE (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Mus musculus GN=Hmgcs1 PE=1 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 1.635e-53
Identity = 94/131 (71.76%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++KS LMQ+FE+ GNTDIEG+D+TNACYGGTAA+FN VNWVES+SWDGRY LVV  D A+YA G ARPTGG  A+A+LIGP+AP+ F+  LRG+HM H YDFYKPD+ SEYPVVDGKLS  CYL ALD CY
Sbjct:  103 SVKSNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWVESSSWDGRYALVVAGDIAIYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPVVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_PONAB (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Pongo abelii GN=HMGCS1 PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 2.136e-53
Identity = 93/131 (70.99%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++K+ LMQ+FE+ GNTDIEG+D+TNACYGGTAA+FN VNW+ES+SWDGRY LVV  D AVYA G ARPTGG  A+A+LIGP+AP+ FE  LRG+HM H YDFYKPD+ SEYP+VDGKLS  CYL ALD CY
Sbjct:  103 SVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_HUMAN (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Homo sapiens GN=HMGCS1 PE=1 SV=2)

HSP 1 Score: 207.223 bits (526), Expect = 2.136e-53
Identity = 93/131 (70.99%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++K+ LMQ+FE+ GNTDIEG+D+TNACYGGTAA+FN VNW+ES+SWDGRY LVV  D AVYA G ARPTGG  A+A+LIGP+AP+ FE  LRG+HM H YDFYKPD+ SEYP+VDGKLS  CYL ALD CY
Sbjct:  103 SVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS1_CRIGR (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Cricetulus griseus GN=HMGCS1 PE=3 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 2.789e-53
Identity = 92/131 (70.23%), Postives = 110/131 (83.97%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            ++KS LMQ+FE+ GNTDIEG+D+TNACYGGTAA+FN VNW+ES+SWDGRY LVV  D A+YA G ARPTGG  A+A+LIGP+AP+ F+  LRG+HM H YDFYKPD+ SEYP+VDGKLS  CYL ALD CY
Sbjct:  103 SVKSNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAIYATGNARPTGGVGAVALLIGPNAPLIFDRGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCY 233
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS2_HUMAN (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Homo sapiens GN=HMGCS2 PE=1 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.060e-52
Identity = 91/133 (68.42%), Postives = 111/133 (83.46%), Query Frame = 3 
Query:    6 IKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKNF 404
            +K+ LM++F+  GNTDIEG+D+TNACYGGTA+LFN  NW+ES+SWDGRY +VVC D AVY  G ARPTGGA A+AMLIGP AP++ E  LRG+HM +VYDFYKP+LASEYP+VDGKLS  CYL ALD CY ++
Sbjct:  141 VKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGRYAMVVCGDIAVYPSGNARPTGGAGAVAMLIGPKAPLALERGLRGTHMENVYDFYKPNLASEYPIVDGKLSIQCYLRALDRCYTSY 273
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS2_BOVIN (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Bos taurus GN=HMGCS2 PE=2 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 5.260e-52
Identity = 90/130 (69.23%), Postives = 108/130 (83.08%), Query Frame = 3 
Query:    6 IKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            +K+ LM++F+  GNTDIEG+D+TNACYGGTA+LFN  NW+ES+SWDGRY LVVC D AVY  G ARPTGGA A+AML+GP+AP+  E  LRG+HM +VYDFYKPD+ SEYP+VDGKLS  CYL ALD CY
Sbjct:  141 VKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGRYALVVCGDIAVYPSGNARPTGGAGAVAMLVGPEAPLVLERGLRGTHMENVYDFYKPDVTSEYPLVDGKLSIQCYLRALDKCY 270
[Close]
BLAST of FG531214 vs. SwissProt
Match: HMCS2_MOUSE (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Mus musculus GN=Hmgcs2 PE=1 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 5.816e-51
Identity = 90/130 (69.23%), Postives = 106/130 (81.54%), Query Frame = 3 
Query:    6 IKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCY 395
            +K+ LM++F+  GNTDIEG+D+TNACYGGTA+LFN  NW+ES+ WDGRY LVVC D AVY  G ARPTGGA A+AMLIGP AP+  E  LRG+HM + YDFYKP+LASEYP+VDGKLS  CYL ALD CY
Sbjct:  141 VKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSYWDGRYALVVCGDIAVYPSGNARPTGGAGAVAMLIGPKAPLVLEQGLRGTHMENAYDFYKPNLASEYPLVDGKLSIQCYLRALDRCY 270
[Close]
BLAST of FG531214 vs. TAIR peptide
Match: AT4G11820.2 (| Symbols: MVA1, FKP1 | hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase | chr4:7109124-7111901 REVERSE LENGTH=461)

HSP 1 Score: 260.766 bits (665), Expect = 1.799e-70
Identity = 121/133 (90.98%), Postives = 129/133 (96.99%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKN 401
            +IK+FLMQ+FEK GNTD+EGVDSTNACYGGTAAL NCVNWVESNSWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAMLIGPDAPI FESKLR SHMAHVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+
Sbjct:   91 SIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKH 223
[Close]
BLAST of FG531214 vs. TAIR peptide
Match: AT4G11820.1 (| Symbols: MVA1, HMGS, EMB2778, FKP1 | hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase | chr4:7109124-7111213 REVERSE LENGTH=406)

HSP 1 Score: 260.766 bits (665), Expect = 1.799e-70
Identity = 121/133 (90.98%), Postives = 129/133 (96.99%), Query Frame = 3 
Query:    3 TIKSFLMQIFEKYGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPISFESKLRGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDTCYKN 401
            +IK+FLMQ+FEK GNTD+EGVDSTNACYGGTAAL NCVNWVESNSWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAMLIGPDAPI FESKLR SHMAHVYDFYKP+LASEYPVVDGKLSQTCYLMALD+CYK+
Sbjct:   36 SIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKH 168
The following BLAST results are available for this feature:
BLAST of FG531214 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q1KT07_MEDTR5.271e-7498.513-hydroxy-3-methylglutaryl CoA synthase OS=Medicag... [more]
Q6QLW8_HEVBR2.292e-6991.04HMG-CoA synthase 2 OS=Hevea brasiliensis GN=HMGS2 ... [more]
A9ZMZ7_HEVBR2.292e-6991.04Hydroxymethylglutaryl-CoA synthase OS=Hevea brasil... [more]
Q94ET0_HEVBR2.993e-6990.30Hydroxymethylglutaryl coenzyme A synthase OS=Hevea... [more]
Q944F8_HEVBR2.993e-6990.30Hydroxymethylglutaryl coenzyme A synthase OS=Hevea... [more]
B9GKQ4_POPTR2.993e-6992.54Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
A9ZMZ6_HEVBR2.993e-6990.30Hydroxymethylglutaryl-CoA synthase OS=Hevea brasil... [more]
A9PBS1_POPTR2.993e-6992.54Putative uncharacterized protein OS=Populus tricho... [more]
B9RC08_RICCO3.909e-6991.04Hydroxymethylglutaryl-CoA synthase, putative OS=Ri... [more]
B9GW51_POPTR5.106e-6991.79Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of FG531214 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
HMCS_ARATH1.628e-6990.98Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis ... [more]
HMCS1_CHICK9.586e-5470.99Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_RAT1.252e-5370.99Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_MOUSE1.635e-5371.76Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_PONAB2.136e-5370.99Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_HUMAN2.136e-5370.99Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_CRIGR2.789e-5370.23Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS2_HUMAN1.060e-5268.42Hydroxymethylglutaryl-CoA synthase, mitochondrial ... [more]
HMCS2_BOVIN5.260e-5269.23Hydroxymethylglutaryl-CoA synthase, mitochondrial ... [more]
HMCS2_MOUSE5.816e-5169.23Hydroxymethylglutaryl-CoA synthase, mitochondrial ... [more]
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BLAST of FG531214 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G11820.21.799e-7090.98| Symbols: MVA1, FKP1 | hydroxymethylglutaryl-CoA ... [more]
AT4G11820.11.799e-7090.98| Symbols: MVA1, HMGS, EMB2778, FKP1 | hydroxymeth... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 59..70
score
NoneNo IPR availablePANTHERPTHR11877HYDROXYMETHYLGLUTARYL-COA SYNTHASEcoord: 2..134
score: 1.9
IPR000590Hydroxymethylglutaryl-coenzyme A synthase, active sitePROSITEPS01226HMG_COA_SYNTHASEcoord: 15..30
score
IPR013528Hydroxymethylglutaryl-coenzyme A synthase, N-terminalPFAMPF01154HMG_CoA_synt_Ncoord: 2..84
score: 1.7
IPR013746Hydroxymethylglutaryl-coenzyme A synthase C-terminalPFAMPF08540HMG_CoA_synt_Ccoord: 85..134
score: 5.6
IPR016038Thiolase-like, subgroupGENE3D3.40.47.10no descriptioncoord: 8..79
score: 1.2
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 85..134
score: 3.8e-15coord: 1..84
score: 1.3

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG531214 ID=FG531214; Name=FG531214; organism=Pisum sativum; type=EST; length=405bp
AAACCATCAAGTCATTCTTGATGCAGATCTTTGAGAAATATGGAAATACT
GACATTGAAGGTGTTGATTCAACTAATGCATGCTATGGAGGAACTGCCGC
TTTGTTCAATTGTGTCAATTGGGTGGAGAGCAATTCATGGGATGGACGCT
ATGGACTTGTTGTCTGCACAGACAGTGCCGTCTATGCTGAAGGTCCTGCT
CGACCTACCGGAGGAGCTGCTGCTATTGCCATGCTAATTGGTCCTGATGC
TCCTATTTCTTTTGAAAGCAAATTGAGAGGAAGTCATATGGCTCATGTTT
ATGATTTTTACAAACCTGACCTTGCTAGTGAATATCCGGTTGTCGACGGA
AAGCTTTCACAAACATGTTACCTCATGGCCCTTGATACTTGCTATAAAAA
TTTCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004421hydroxymethylglutaryl-CoA synthase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008299isoprenoid biosynthetic process
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR000590HMG_CoA_synt_AS
IPR013528HMG_CoA_synth_N
IPR013746HMG_CoA_synt_C_dom
IPR016038Thiolase-like, subgroup
IPR016039Thiolase-like