FG531764

EST Overview


Name	FG531764
Unique Name	FG531764
Type	EST
Organism	Pisum sativum (pea)
Sequence length	413

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG531764 vs. TrEMBL
Match: B9S0K1_RICCO (Isocitrate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1355470 PE=3 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 3.736e-64
Identity = 119/130 (91.54%), Postives = 128/130 (98.46%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTAL+LSSVTMLRH++LHDKADRIQ+AIL+TI+EGKYRTADLGG+S TT+FTKAI DH+
Sbjct:  235 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALMLSSVTMLRHLELHDKADRIQSAILDTISEGKYRTADLGGSSSTTDFTKAICDHL 364

[Close]

BLAST of FG531764 vs. TrEMBL
Match: O65852_TOBAC (Isocitrate dehydrogenase (NAD+) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.087e-63
Identity = 117/130 (90.00%), Postives = 125/130 (96.15%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFD+LVMPNLYGDIISDLCAG+IGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLS+VTMLRH++LHDKADRIQ A+LNTIAEGKYRT DLGGTS TT+FT AI DH+
Sbjct:  235 DNCCMMLVKNPALFDILVMPNLYGDIISDLCAGVIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRHLELHDKADRIQGAVLNTIAEGKYRTGDLGGTSSTTDFTNAICDHL 364

[Close]

BLAST of FG531764 vs. TrEMBL
Match: D7TQM9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_40.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025704001 PE=4 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.854e-63
Identity = 117/130 (90.00%), Postives = 127/130 (97.69%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKN+ANPTAL+LS+VTMLRH+ LHDKAD+IQNAILNTI+EG+YRTADLGG+S TT+FTKAI DH+
Sbjct:  237 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALMLSAVTMLRHLKLHDKADQIQNAILNTISEGRYRTADLGGSSTTTDFTKAICDHL 366

[Close]

BLAST of FG531764 vs. TrEMBL
Match: B9MTD0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_589452 PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 7.045e-63
Identity = 118/130 (90.77%), Postives = 126/130 (96.92%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLS+VTMLRH++L+DKA+RIQNAIL TIAEGKYRTADLGG+S TT+FTKAI  H+
Sbjct:  231 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRHLELYDKAERIQNAILTTIAEGKYRTADLGGSSSTTDFTKAICGHL 360

[Close]

BLAST of FG531764 vs. TrEMBL
Match: B9IFP1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_825258 PE=3 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 3.496e-62
Identity = 116/130 (89.23%), Postives = 126/130 (96.92%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE GIALAEAVHGSAPDIAGKNLANPTALLLS+VTMLRH++L+DKA+RIQ+AILNTIAEGKYRTADLGG+S TT+FTKAI  H+
Sbjct:  234 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEAGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRHLELYDKAERIQDAILNTIAEGKYRTADLGGSSSTTDFTKAICGHL 363

[Close]

BLAST of FG531764 vs. TrEMBL
Match: A9P904_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 3.496e-62
Identity = 116/130 (89.23%), Postives = 126/130 (96.92%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE GIALAEAVHGSAPDIAGKNLANPTALLLS+VTMLRH++L+DKA+RIQ+AILNTIAEGKYRTADLGG+S TT+FTKAI  H+
Sbjct:  234 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEAGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRHLELYDKAERIQDAILNTIAEGKYRTADLGGSSSTTDFTKAICGHL 363

[Close]

BLAST of FG531764 vs. TrEMBL
Match: D7LWM4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487101 PE=4 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.624e-59
Identity = 111/130 (85.38%), Postives = 123/130 (94.62%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVHGSAPDIAGKNLANPTALLLS V MLRH+ L+++A++I +AI+NTIAEGKYRTADLGG+S TTEFTKAI DH+
Sbjct:  244 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKLNEQAEQIHSAIINTIAEGKYRTADLGGSSTTTEFTKAICDHL 373

[Close]

BLAST of FG531764 vs. TrEMBL
Match: Q84JA9_BRANA (NAD-dependent isocitrate dehydrogenase alpha subunit (Fragment) OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 4.726e-59
Identity = 110/130 (84.62%), Postives = 122/130 (93.85%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVHGSAPDIAGKNLANPTALLLS V MLRH+ L+++A++I  AI+NTIAEGKYRTADLGG+S TT+FTKAI DH+
Sbjct:  201 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKLNEQAEQIHRAIINTIAEGKYRTADLGGSSTTTDFTKAICDHL 330

[Close]

BLAST of FG531764 vs. TrEMBL
Match: C5XH85_SORBI (Putative uncharacterized protein Sb03g011050 OS=Sorghum bicolor GN=Sb03g011050 PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.375e-58
Identity = 110/130 (84.62%), Postives = 118/130 (90.77%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCM LVKNP LFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI LAEAVHGSAPDIAGKNLANPTAL+LS+V MLRH+  +DKADRI NAIL TIAEGKYRTADLGG + T+EFT A+ +HI
Sbjct:  236 DNCCMTLVKNPGLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGICLAEAVHGSAPDIAGKNLANPTALMLSAVMMLRHLQFNDKADRIHNAILQTIAEGKYRTADLGGKASTSEFTNAVCEHI 365

[Close]

BLAST of FG531764 vs. TrEMBL
Match: B6TJM1_MAIZE (3-isopropylmalate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.375e-58
Identity = 110/130 (84.62%), Postives = 118/130 (90.77%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCM LVKNP LFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI LAEAVHGSAPDIAGKNLANPTAL+LS+V +LRH+  +DKADRI NAIL TIAEGKYRTADLGG + T+EFT A+ DHI
Sbjct:  236 DNCCMTLVKNPGLFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGICLAEAVHGSAPDIAGKNLANPTALMLSAVMLLRHMQFNDKADRIHNAILQTIAEGKYRTADLGGKASTSEFTNAVCDHI 365

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH5_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis thaliana GN=IDH5 PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 2.328e-60
Identity = 110/130 (84.62%), Postives = 122/130 (93.85%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVHGSAPDIAGKNLANPTALLLS V MLRH+  +++A++I +AI+NTIAEGKYRTADLGG+S TTEFTKAI DH+
Sbjct:  245 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSSTTTEFTKAICDHL 374

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH6_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis thaliana GN=IDH6 PE=1 SV=2)

HSP 1 Score: 223.787 bits (569), Expect = 2.179e-58
Identity = 109/130 (83.85%), Postives = 120/130 (92.31%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEAVHGSAPDIAG NLANPTALLLS V MLRH+ L+ +A++I +AI+NTIAEGKYRTADLGG+S TT+FTKAI DH+
Sbjct:  245 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEAVHGSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGSSTTTDFTKAICDHL 374

[Close]

BLAST of FG531764 vs. SwissProt
Match: LEU3_SOLTU (3-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 3.600e-53
Identity = 101/107 (94.39%), Postives = 105/107 (98.13%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGK 323
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSV+MLRH++LHDKADRIQ+AIL TIA GK
Sbjct:  238 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDKADRIQDAILKTIAGGK 344

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDHA_DICDI (Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostelium discoideum GN=idhA PE=3 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 2.586e-43
Identity = 84/130 (64.62%), Postives = 102/130 (78.46%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DN CM LV +P   DV+V+PNLYGDI+SDLCAGLIGGLGLTPS NIGE G A+ EAVHG+APDIAGKN ANPTAL+LSS+ MLRH+    +A  I+NA+LNT+ EGK +T DLGG S  +E+T  ++  I
Sbjct:  219 DNNCMQLVLDPNQMDVMVLPNLYGDIVSDLCAGLIGGLGLTPSGNIGENGSAIFEAVHGTAPDIAGKNKANPTALILSSIMMLRHLGHFHEASIIENAVLNTLTEGKVKTGDLGGNSSCSEYTDELVKKI 348

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_DROME (Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophila melanogaster GN=l(1)G0156 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 6.369e-42
Identity = 83/126 (65.87%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V+NP  +DVLVMPNLYGDI+SD+CAGL+GGLGLTPS N+G  G AL E+VHG+APDIAGK+LANPTALLLS+V MLRH++L+  AD+I+ A   TI EGKY T DLGG +K +EFT  I
Sbjct:  249 DTVCLNMVQNPGKYDVLVMPNLYGDILSDMCAGLVGGLGLTPSGNMGLNG-ALFESVHGTAPDIAGKDLANPTALLLSAVMMLRHMELNTYADKIERAAFETIKEGKYLTGDLGGRAKCSEFTNEI 373

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_BOVIN (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Bos taurus GN=IDH3A PE=1 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 1.419e-41
Identity = 81/126 (64.29%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V++P+ FDVLVMPNLYGDI+SDLCAGLIGGLG+TPS NIG  G+A+ E+VHG+APDIAGK++ANPTALLLS+V MLRH+ L D A +I+ A   TI +GK  T DLGG SK ++FT+ I
Sbjct:  233 DTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNSKCSDFTEEI 358

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_PONAB (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Pongo abelii GN=IDH3A PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 2.420e-41
Identity = 81/126 (64.29%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V++P+ FDVLVMPNLYGDI+SDLCAGLIGGLG+TPS NIG  G+A+ E+VHG+APDIAGK++ANPTALLLS+V MLRH+ L D A RI+ A   TI +GK  T DLGG +K ++FT+ I
Sbjct:  233 DTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEI 358

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_MACFA (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (Fragment) OS=Macaca fascicularis GN=IDH3A PE=2 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 2.420e-41
Identity = 81/126 (64.29%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V++P+ FDVLVMPNLYGDI+SDLCAGLIGGLG+TPS NIG  G+A+ E+VHG+APDIAGK++ANPTALLLS+V MLRH+ L D A RI+ A   TI +GK  T DLGG +K ++FT+ I
Sbjct:  214 DTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEI 339

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_HUMAN (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Homo sapiens GN=IDH3A PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 2.420e-41
Identity = 81/126 (64.29%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V++P+ FDVLVMPNLYGDI+SDLCAGLIGGLG+TPS NIG  G+A+ E+VHG+APDIAGK++ANPTALLLS+V MLRH+ L D A RI+ A   TI +GK  T DLGG +K ++FT+ I
Sbjct:  233 DTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEI 358

[Close]

BLAST of FG531764 vs. SwissProt
Match: IDH3A_MOUSE (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Mus musculus GN=Idh3a PE=1 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 5.392e-41
Identity = 80/126 (63.49%), Postives = 102/126 (80.95%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAI 380
            D  C+ +V++P+ FDVLVMPNLYGDI+SDLCAGLIGGLG+TPS NIG  G+A+ E+VHG+APDIAGK++ANPTALLLS+V MLRH+ L D A +I+ A   TI +GK  T DLGG +K ++FT+ I
Sbjct:  233 DTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIEAACFATIKDGKSLTKDLGGNAKCSDFTEEI 358

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT5G03290.1 (| Symbols: IDH-V | isocitrate dehydrogenase V | chr5:794043-795939 FORWARD LENGTH=374)

HSP 1 Score: 230.335 bits (586), Expect = 2.711e-61
Identity = 110/130 (84.62%), Postives = 122/130 (93.85%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVHGSAPDIAGKNLANPTALLLS V MLRH+  +++A++I +AI+NTIAEGKYRTADLGG+S TTEFTKAI DH+
Sbjct:  245 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSSTTTEFTKAICDHL 374

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT3G09810.1 (| Symbols: IDH-VI | isocitrate dehydrogenase VI | chr3:3008753-3011070 FORWARD LENGTH=374)

HSP 1 Score: 223.787 bits (569), Expect = 2.537e-59
Identity = 109/130 (83.85%), Postives = 120/130 (92.31%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEAVHGSAPDIAG NLANPTALLLS V MLRH+ L+ +A++I +AI+NTIAEGKYRTADLGG+S TT+FTKAI DH+
Sbjct:  245 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEAVHGSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGSSTTTDFTKAICDHL 374

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT4G35260.1 (| Symbols: IDH1, IDH-I | isocitrate dehydrogenase 1 | chr4:16774494-16776233 REVERSE LENGTH=367)

HSP 1 Score: 119.783 bits (299), Expect = 5.160e-28
Identity = 63/130 (48.46%), Postives = 79/130 (60.77%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIAL---AEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAII 383
            DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG G+ P  N+G         A A +     I  +N ANP ALLLSS  MLRH+     ADR++ A+   IAEGK RT DLGGTS T E   A+I
Sbjct:  234 DNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQEVVDAVI 363

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT4G35650.1 (| Symbols: IDH-III | isocitrate dehydrogenase III | chr4:16908584-16909973 FORWARD LENGTH=368)

HSP 1 Score: 113.235 bits (282), Expect = 4.829e-26
Identity = 62/130 (47.69%), Postives = 79/130 (60.77%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIG-EGGIALAEAVHGSAPD--IAGKNLANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAII 383
            DNCCM LV  P  FDV+V PNLYG++I++  AG+ GG G+ P  N+G E  I    A  G+  +  +  +  ANP ALLLSS  MLRH+     ADR++ A+   I EGKYRT DLGG   T E   A+I
Sbjct:  235 DNCCMQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNVGNDKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQEVVDAVI 364

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT2G17130.2 (| Symbols: IDH2, IDH-II | isocitrate dehydrogenase subunit 2 | chr2:7461062-7462466 REVERSE LENGTH=363)

HSP 1 Score: 113.235 bits (282), Expect = 4.829e-26
Identity = 61/136 (44.85%), Postives = 80/136 (58.82%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNL------ANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG G+ P  N+G      A    G++    GK+       ANP ALLLSS  MLRH+     ADR++ A+   IAEG  RT DLGG S T E   A+I ++
Sbjct:  230 DNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGA---EYAVFEQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANL 362

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT2G17130.1 (| Symbols: IDH2, IDH-II | isocitrate dehydrogenase subunit 2 | chr2:7461062-7462466 REVERSE LENGTH=367)

HSP 1 Score: 113.235 bits (282), Expect = 4.829e-26
Identity = 61/136 (44.85%), Postives = 80/136 (58.82%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNL------ANPTALLLSSVTMLRHIDLHDKADRIQNAILNTIAEGKYRTADLGGTSKTTEFTKAIIDHI 392
            DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG G+ P  N+G      A    G++    GK+       ANP ALLLSS  MLRH+     ADR++ A+   IAEG  RT DLGG S T E   A+I ++
Sbjct:  234 DNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGA---EYAVFEQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANL 366

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT5G14200.3 (| Symbols: | isopropylmalate dehydrogenase 1 | chr5:4576220-4577962 FORWARD LENGTH=388)

HSP 1 Score: 98.9821 bits (245), Expect = 9.425e-22
Identity = 47/114 (41.23%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRH-IDLHDKADRIQNAILNTIAEGKYRTADL 341
            DN  M L+++P  FD +V  N++GDI+SD  + + G +G+ PS ++GE G  L E +HGSAPDIAG++ ANP A +LS+  +L++ +     A RI++A+++ + +G +RT D+
Sbjct:  268 DNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKG-FRTGDI 380

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT5G14200.1 (| Symbols: ATIMD1, IMD1 | isopropylmalate dehydrogenase 1 | chr5:4576220-4578111 FORWARD LENGTH=409)

HSP 1 Score: 98.9821 bits (245), Expect = 9.425e-22
Identity = 47/114 (41.23%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRH-IDLHDKADRIQNAILNTIAEGKYRTADL 341
            DN  M L+++P  FD +V  N++GDI+SD  + + G +G+ PS ++GE G  L E +HGSAPDIAG++ ANP A +LS+  +L++ +     A RI++A+++ + +G +RT D+
Sbjct:  268 DNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKG-FRTGDI 380

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT1G31180.1 (| Symbols: ATIMD3, IMD3, IPMDH1 | isopropylmalate dehydrogenase 3 | chr1:11142843-11144617 REVERSE LENGTH=404)

HSP 1 Score: 97.4413 bits (241), Expect = 2.742e-21
Identity = 48/114 (42.11%), Postives = 79/114 (69.30%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHIDLHDKADR-IQNAILNTIAEGKYRTADL 341
            DN  M LV++P  FD +V  N++GDI+SD  + + G +G+ PS ++GE G  L E +HGSAPDIAG++ ANP A +LS+  +L++    +KA + I++A+++ + +G +RT D+
Sbjct:  265 DNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKMIEDAVVDALNKG-FRTGDI 377

[Close]

BLAST of FG531764 vs. TAIR peptide
Match: AT1G80560.1 (| Symbols: ATIMD2, IMD2 | isopropylmalate dehydrogenase 2 | chr1:30287833-30290126 FORWARD LENGTH=405)

HSP 1 Score: 95.1301 bits (235), Expect = 1.361e-20
Identity = 47/114 (41.23%), Postives = 75/114 (65.79%), Query Frame = 3

Query:    3 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRH-IDLHDKADRIQNAILNTIAEGKYRTADL 341
            DN  M LV++P  FD +V  N++GDI+SD  + + G +G+ PS ++ + G  L E +HGSAPDIAG++ ANP A +LS+  +L++ +     A RI++A+L  +  G +RT D+
Sbjct:  264 DNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVLVALNNG-FRTGDI 376

The following BLAST results are available for this feature:

BLAST of FG531764 vs. TrEMBL
BLAST of FG531764 vs. SwissProt
BLAST of FG531764 vs. TAIR peptide

BLAST of FG531764 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S0K1_RICCO	3.736e-64	91.54	Isocitrate dehydrogenase, putative OS=Ricinus comm...	[more]
O65852_TOBAC	1.087e-63	90.00	Isocitrate dehydrogenase (NAD+) OS=Nicotiana tabac...	[more]
D7TQM9_VITVI	1.854e-63	90.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9MTD0_POPTR	7.045e-63	90.77	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IFP1_POPTR	3.496e-62	89.23	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9P904_POPTR	3.496e-62	89.23	Putative uncharacterized protein OS=Populus tricho...	[more]
D7LWM4_ARALY	1.624e-59	85.38	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q84JA9_BRANA	4.726e-59	84.62	NAD-dependent isocitrate dehydrogenase alpha subun...	[more]
C5XH85_SORBI	1.375e-58	84.62	Putative uncharacterized protein Sb03g011050 OS=So...	[more]
B6TJM1_MAIZE	1.375e-58	84.62	3-isopropylmalate dehydrogenase OS=Zea mays PE=2 S...	[more]

BLAST of FG531764 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
IDH5_ARATH	2.328e-60	84.62	Isocitrate dehydrogenase [NAD] catalytic subunit 5...	[more]
IDH6_ARATH	2.179e-58	83.85	Isocitrate dehydrogenase [NAD] catalytic subunit 6...	[more]
LEU3_SOLTU	3.600e-53	94.39	3-isopropylmalate dehydrogenase, chloroplastic OS=...	[more]
IDHA_DICDI	2.586e-43	64.62	Isocitrate dehydrogenase [NAD] regulatory subunit ...	[more]
IDH3A_DROME	6.369e-42	65.87	Probable isocitrate dehydrogenase [NAD] subunit al...	[more]
IDH3A_BOVIN	1.419e-41	64.29	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_PONAB	2.420e-41	64.29	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_MACFA	2.420e-41	64.29	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_HUMAN	2.420e-41	64.29	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_MOUSE	5.392e-41	63.49	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]

BLAST of FG531764 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G03290.1	2.711e-61	84.62	\| Symbols: IDH-V \| isocitrate dehydrogenase V \| ch...	[more]
AT3G09810.1	2.537e-59	83.85	\| Symbols: IDH-VI \| isocitrate dehydrogenase VI \| ...	[more]
AT4G35260.1	5.160e-28	48.46	\| Symbols: IDH1, IDH-I \| isocitrate dehydrogenase ...	[more]
AT4G35650.1	4.829e-26	47.69	\| Symbols: IDH-III \| isocitrate dehydrogenase III ...	[more]
AT2G17130.2	4.829e-26	44.85	\| Symbols: IDH2, IDH-II \| isocitrate dehydrogenase...	[more]
AT2G17130.1	4.829e-26	44.85	\| Symbols: IDH2, IDH-II \| isocitrate dehydrogenase...	[more]
AT5G14200.3	9.425e-22	41.23	\| Symbols: \| isopropylmalate dehydrogenase 1 \| ch...	[more]
AT5G14200.1	9.425e-22	41.23	\| Symbols: ATIMD1, IMD1 \| isopropylmalate dehydrog...	[more]
AT1G31180.1	2.742e-21	42.11	\| Symbols: ATIMD3, IMD3, IPMDH1 \| isopropylmalate ...	[more]
AT1G80560.1	1.361e-20	41.23	\| Symbols: ATIMD2, IMD2 \| isopropylmalate dehydrog...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 39..50 score
None	No IPR available	PANTHER	PTHR11835:SF5	ISOCITRATE DEHYDROGENASE [NAD]	coord: 1..130 score: 1.2
None	No IPR available	SUPERFAMILY	53659	Isocitrate/Isopropylmalate dehydrogenase-like	coord: 1..130 score: 5.2
IPR001804	Isocitrate/isopropylmalate dehydrogenase	GENE3D	3.40.718.10	no description	coord: 1..130 score: 2.8
IPR001804	Isocitrate/isopropylmalate dehydrogenase	PANTHER	PTHR11835	DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE	coord: 1..130 score: 1.2
IPR001804	Isocitrate/isopropylmalate dehydrogenase	PFAM	PF00180	Iso_dh	coord: 1..126 score: 2.3
IPR019818	Isocitrate/isopropylmalate dehydrogenase, conserved site	PROSITE	PS00470	IDH_IMDH	coord: 21..40 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531764 ID=FG531764; Name=FG531764; organism=Pisum sativum; type=EST; length=413bp
TTGACAATTGCTGCATGATGCTTGTGAAAAATCCTGCACTTTTTGATGTG
TTGGTGATGCCTAATCTTTATGGTGACATAATCAGTGACCTTTGTGCTGG
CTTGATTGGAGGATTAGGCTTGACACCCAGCTGCAACATTGGCGAGGGAG
GTATTGCACTTGCTGAGGCTGTACATGGTTCAGCACCTGATATTGCCGGG
AAGAATTTGGCTAATCCAACTGCTTTGCTGCTAAGTTCGGTTACTATGTT
GCGCCATATCGATCTCCATGACAAAGCAGACCGAATTCAAAATGCCATCC
TCAACACAATTGCAGAAGGGAAGTACCGAACTGCTGATCTCGGTGGCACT
TCAAAGACAACTGAATTTACAAAAGCAATTATTGATCATATTTGATTTTT
GCTTTCAAGTTTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0051287	NAD binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001804	Isocitrate/isopropylmalate_DH
IPR019818	IsoCit/isopropylmalate_DH_CS

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