GH719683

EST Overview
NameGH719683
Unique NameGH719683
TypeEST
OrganismPisum sativum (pea)
Sequence length359
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Libraries
Library NameType
LIBEST_024268 TIcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of GH719683 vs. TrEMBL
Match: Q9M638_MEDTR (Malate dehydrogenase (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.540e-15
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR+GVEEFLPLGPLSDYERASLEKAKKELATSVEKG+SFIRK
Sbjct:   24 LGRNGVEEFLPLGPLSDYERASLEKAKKELATSVEKGVSFIRK 66
[Close]
BLAST of GH719683 vs. TrEMBL
Match: Q9M601_MEDTR (Malate dehydrogenase (Fragment) OS=Medicago truncatula GN=mdh PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.540e-15
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR+GVEEFLPLGPLSDYERASLEKAKKELATSVEKG+SFIRK
Sbjct:   18 LGRNGVEEFLPLGPLSDYERASLEKAKKELATSVEKGVSFIRK 60
[Close]
BLAST of GH719683 vs. TrEMBL
Match: O48903_MEDSA (Malate dehydrogenase OS=Medicago sativa GN=gmdh PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.540e-15
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR+GVEEFLPLGPLSDYERASLEKAKKELATSVEKG+SFIRK
Sbjct:  316 LGRNGVEEFLPLGPLSDYERASLEKAKKELATSVEKGVSFIRK 358
[Close]
BLAST of GH719683 vs. TrEMBL
Match: Q9SMJ6_CICAR (Malate dehydrogenase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.690e-14
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGRDGVEEFLPLGPLSDYER SLEKAKKELATSVEKGISF R+
Sbjct:  160 LGRDGVEEFLPLGPLSDYERESLEKAKKELATSVEKGISFTRQ 202
[Close]
BLAST of GH719683 vs. TrEMBL
Match: C6T6I4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.716e-11
Identity = 35/43 (81.40%), Postives = 37/43 (86.05%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR GVEE  PLGPL+DYER SLEKAKKELA S+EKGI FIRK
Sbjct:   44 LGRVGVEEIFPLGPLNDYERESLEKAKKELAASIEKGIFFIRK 86
[Close]
BLAST of GH719683 vs. TrEMBL
Match: B9HFT0_POPTR (Malate dehydrogenase OS=Populus trichocarpa GN=POPTRDRAFT_820117 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.716e-11
Identity = 32/43 (74.42%), Postives = 39/43 (90.70%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR+GVEE  PLGPL+DYERA LEKAKKELA S++KG+SF++K
Sbjct:  312 LGRNGVEEIYPLGPLNDYERAGLEKAKKELAGSIQKGVSFVKK 354
[Close]
BLAST of GH719683 vs. TrEMBL
Match: D7TG04_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_373.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00006474001 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.339e-11
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE  PLGPL++YERA LEKAKKELA+S++KGISFIRK
Sbjct:  314 LGRTGAEEIYPLGPLNEYERAGLEKAKKELASSIQKGISFIRK 356
[Close]
BLAST of GH719683 vs. TrEMBL
Match: C5YSY8_SORBI (Malate dehydrogenase OS=Sorghum bicolor GN=Sb08g022770 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.146e-10
Identity = 31/43 (72.09%), Postives = 38/43 (88.37%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G+EE LPLGPL+++ERA LEKAKKELA S++KG+SFI K
Sbjct:  323 LGRCGIEEILPLGPLNEFERAGLEKAKKELAESIQKGVSFINK 365
[Close]
BLAST of GH719683 vs. TrEMBL
Match: C0LL36_BAMOL (Malate dehydrogenase OS=Bambusa oldhamii GN=MDH PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.366e-10
Identity = 30/43 (69.77%), Postives = 37/43 (86.05%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G+EE LPLGPL+++ERA LEKAKKEL  S++KG+SFI K
Sbjct:  315 LGRHGIEEILPLGPLNEFERAGLEKAKKELGQSIQKGVSFINK 357
[Close]
BLAST of GH719683 vs. TrEMBL
Match: Q7XZE9_WHEAT (Malate dehydrogenase (Fragment) OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.153e-10
Identity = 30/43 (69.77%), Postives = 37/43 (86.05%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G E+ LPLGPL+D+ERA LEKAKKEL+ S+EKG+SF+ K
Sbjct:  203 LGRGGAEKILPLGPLNDFERAGLEKAKKELSESIEKGVSFMNK 245
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG_SOYBN (Malate dehydrogenase, glyoxysomal OS=Glycine max PE=2 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 1.255e-13
Identity = 37/43 (86.05%), Postives = 39/43 (90.70%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR GVEE LPLGPL+DYER SLEKAKKELA S+EKGISFIRK
Sbjct:  311 LGRVGVEEILPLGPLNDYERESLEKAKKELAASIEKGISFIRK 353
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG_CITLA (Malate dehydrogenase, glyoxysomal OS=Citrullus lanatus PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.697e-10
Identity = 30/42 (71.43%), Postives = 36/42 (85.71%), Query Frame = -3 
Query:  232 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIR 357
            LGR+G+EE   LGPL++YER  LEKAKKELA S+EKG+SFIR
Sbjct:  314 LGRNGIEEVYSLGPLNEYERIGLEKAKKELAGSIEKGVSFIR 355
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG_ORYSJ (Malate dehydrogenase, glyoxysomal OS=Oryza sativa subsp. japonica GN=Os12g0632700 PE=1 SV=3)

HSP 1 Score: 63.929 bits (154), Expect = 2.894e-10
Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G+EE L LGPL+++ERA LEKAKKELA S++KG++FI K
Sbjct:  314 LGRCGIEEILSLGPLNEFERAGLEKAKKELAESIQKGVAFINK 356
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG_CUCSA (Malate dehydrogenase, glyoxysomal OS=Cucumis sativus GN=MDHG PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.780e-10
Identity = 29/42 (69.05%), Postives = 36/42 (85.71%), Query Frame = -3 
Query:  232 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIR 357
            LGR+G++E   LGPL++YER  LEKAKKELA S+EKG+SFIR
Sbjct:  314 LGRNGIDEVYSLGPLNEYERIGLEKAKKELAGSIEKGVSFIR 355
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG2_BRANA (Malate dehydrogenase 2, glyoxysomal OS=Brassica napus GN=MDH2 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.876e-9
Identity = 28/43 (65.12%), Postives = 34/43 (79.07%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE   LGPL++YER  LEKAK+ELA S++KG+ FIRK
Sbjct:  316 LGRTGAEEVFQLGPLNEYERVGLEKAKEELAGSIQKGVDFIRK 358
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG1_BRANA (Malate dehydrogenase 1, glyoxysomal OS=Brassica napus GN=MDH1 PE=2 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 1.876e-9
Identity = 28/43 (65.12%), Postives = 34/43 (79.07%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE   LGPL++YER  LEKAK+ELA S++KG+ FIRK
Sbjct:  316 LGRTGAEEVFQLGPLNEYERVGLEKAKEELAGSIQKGVDFIRK 358
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG1_ARATH (Malate dehydrogenase, glyoxysomal OS=Arabidopsis thaliana GN=At5g09660 PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.458e-9
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE   LGPL++YER  LEKAK ELA S++KG+ FIRK
Sbjct:  312 LGRTGAEEVYQLGPLNEYERIGLEKAKDELAGSIQKGVEFIRK 354
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHG2_ARATH (Probable malate dehydrogenase, glyoxysomal OS=Arabidopsis thaliana GN=At2g22780 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.538e-8
Identity = 25/43 (58.14%), Postives = 34/43 (79.07%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G++E   LGPL++YER  LEKAKKEL+ S+ KG++F +K
Sbjct:  312 LGRCGIDEVYGLGPLNEYERMGLEKAKKELSVSIHKGVTFAKK 354
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHM_CITLA (Malate dehydrogenase, mitochondrial OS=Citrullus lanatus GN=MMDH PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.995e-7
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = -3 
Query:  238 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISF 357
            LG++GVE  L LGPLSD+E+  LEK K EL  S+EKGI F
Sbjct:  304 LGKNGVESVLDLGPLSDFEKEGLEKLKPELKASIEKGIQF 343
[Close]
BLAST of GH719683 vs. SwissProt
Match: MDHM2_ARATH (Malate dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=At3g15020 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.995e-7
Identity = 25/40 (62.50%), Postives = 31/40 (77.50%), Query Frame = -3 
Query:  238 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISF 357
            LG++GVEE L LGPLSD+E+  LE  K EL +S+EKGI F
Sbjct:  299 LGKNGVEEVLDLGPLSDFEKEGLEALKAELKSSIEKGIKF 338
[Close]
BLAST of GH719683 vs. TAIR peptide
Match: AT5G09660.2 (| Symbols: PMDH2 | peroxisomal NAD-malate dehydrogenase 2 | chr5:2993645-2995169 REVERSE LENGTH=333)

HSP 1 Score: 59.6918 bits (143), Expect = 4.355e-10
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE   LGPL++YER  LEKAK ELA S++KG+ FIRK
Sbjct:  291 LGRTGAEEVYQLGPLNEYERIGLEKAKDELAGSIQKGVEFIRK 333
[Close]
BLAST of GH719683 vs. TAIR peptide
Match: AT5G09660.1 (| Symbols: PMDH2 | peroxisomal NAD-malate dehydrogenase 2 | chr5:2993645-2995551 REVERSE LENGTH=354)

HSP 1 Score: 59.6918 bits (143), Expect = 4.355e-10
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G EE   LGPL++YER  LEKAK ELA S++KG+ FIRK
Sbjct:  312 LGRTGAEEVYQLGPLNEYERIGLEKAKDELAGSIQKGVEFIRK 354
[Close]
BLAST of GH719683 vs. TAIR peptide
Match: AT2G22780.1 (| Symbols: PMDH1 | peroxisomal NAD-malate dehydrogenase 1 | chr2:9689995-9691923 REVERSE LENGTH=354)

HSP 1 Score: 56.9954 bits (136), Expect = 2.823e-9
Identity = 25/43 (58.14%), Postives = 34/43 (79.07%), Query Frame = -3 
Query:  229 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISFIRK 357
            LGR G++E   LGPL++YER  LEKAKKEL+ S+ KG++F +K
Sbjct:  312 LGRCGIDEVYGLGPLNEYERMGLEKAKKELSVSIHKGVTFAKK 354
[Close]
BLAST of GH719683 vs. TAIR peptide
Match: AT3G15020.1 (| Symbols: mMDH2 | Lactate/malate dehydrogenase family protein | chr3:5056139-5057941 FORWARD LENGTH=341)

HSP 1 Score: 53.9138 bits (128), Expect = 2.390e-8
Identity = 25/40 (62.50%), Postives = 31/40 (77.50%), Query Frame = -3 
Query:  238 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISF 357
            LG++GVEE L LGPLSD+E+  LE  K EL +S+EKGI F
Sbjct:  299 LGKNGVEEVLDLGPLSDFEKEGLEALKAELKSSIEKGIKF 338
[Close]
BLAST of GH719683 vs. TAIR peptide
Match: AT1G53240.1 (| Symbols: mMDH1 | Lactate/malate dehydrogenase family protein | chr1:19854966-19856802 REVERSE LENGTH=341)

HSP 1 Score: 53.5286 bits (127), Expect = 3.121e-8
Identity = 24/40 (60.00%), Postives = 31/40 (77.50%), Query Frame = -3 
Query:  238 LGRDGVEEFLPLGPLSDYERASLEKAKKELATSVEKGISF 357
            LG++GVEE L LGPLSD+E+  LE  K EL +S+EKG+ F
Sbjct:  299 LGKNGVEEVLDLGPLSDFEKEGLEALKPELKSSIEKGVKF 338
The following BLAST results are available for this feature:
BLAST of GH719683 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9M638_MEDTR5.540e-1595.35Malate dehydrogenase (Fragment) OS=Medicago trunca... [more]
Q9M601_MEDTR5.540e-1595.35Malate dehydrogenase (Fragment) OS=Medicago trunca... [more]
O48903_MEDSA5.540e-1595.35Malate dehydrogenase OS=Medicago sativa GN=gmdh PE... [more]
Q9SMJ6_CICAR4.690e-1493.02Malate dehydrogenase (Fragment) OS=Cicer arietinum... [more]
C6T6I4_SOYBN3.716e-1181.40Putative uncharacterized protein OS=Glycine max PE... [more]
B9HFT0_POPTR3.716e-1174.42Malate dehydrogenase OS=Populus trichocarpa GN=POP... [more]
D7TG04_VITVI6.339e-1176.74Whole genome shotgun sequence of line PN40024, sca... [more]
C5YSY8_SORBI3.146e-1072.09Malate dehydrogenase OS=Sorghum bicolor GN=Sb08g02... [more]
C0LL36_BAMOL5.366e-1069.77Malate dehydrogenase OS=Bambusa oldhamii GN=MDH PE... [more]
Q7XZE9_WHEAT9.153e-1069.77Malate dehydrogenase (Fragment) OS=Triticum aestiv... [more]
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BLAST of GH719683 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
MDHG_SOYBN1.255e-1386.05Malate dehydrogenase, glyoxysomal OS=Glycine max P... [more]
MDHG_CITLA1.697e-1071.43Malate dehydrogenase, glyoxysomal OS=Citrullus lan... [more]
MDHG_ORYSJ2.894e-1067.44Malate dehydrogenase, glyoxysomal OS=Oryza sativa ... [more]
MDHG_CUCSA3.780e-1069.05Malate dehydrogenase, glyoxysomal OS=Cucumis sativ... [more]
MDHG2_BRANA1.876e-965.12Malate dehydrogenase 2, glyoxysomal OS=Brassica na... [more]
MDHG1_BRANA1.876e-965.12Malate dehydrogenase 1, glyoxysomal OS=Brassica na... [more]
MDHG1_ARATH5.458e-965.12Malate dehydrogenase, glyoxysomal OS=Arabidopsis t... [more]
MDHG2_ARATH3.538e-858.14Probable malate dehydrogenase, glyoxysomal OS=Arab... [more]
MDHM_CITLA2.995e-762.50Malate dehydrogenase, mitochondrial OS=Citrullus l... [more]
MDHM2_ARATH2.995e-762.50Malate dehydrogenase 2, mitochondrial OS=Arabidops... [more]
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BLAST of GH719683 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G09660.24.355e-1065.12| Symbols: PMDH2 | peroxisomal NAD-malate dehydrog... [more]
AT5G09660.14.355e-1065.12| Symbols: PMDH2 | peroxisomal NAD-malate dehydrog... [more]
AT2G22780.12.823e-958.14| Symbols: PMDH1 | peroxisomal NAD-malate dehydrog... [more]
AT3G15020.12.390e-862.50| Symbols: mMDH2 | Lactate/malate dehydrogenase fa... [more]
AT1G53240.13.121e-860.00| Symbols: mMDH1 | Lactate/malate dehydrogenase fa... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH719683 ID=GH719683; Name=GH719683; organism=Pisum sativum; type=EST; length=359bp
TTGAATATTTCTTTTAATCAAAGCTCAAATGGCCACGTTTATACTTCACA
AATTTTAAAAAATAAATATCTATATTGTCAAAAAAATAAACTATGTATTT
AAATTAAACACACTCTTTTATTGTAATATGAATGGTTTTTACTAGTATAT
AACTAATTTGAAAATTTTGATCTCACAACTGACATGACTTAATTCCACCA
CTTATTTTCAGGATATGCTATCAACTCATTTTCTGATGAAAGAAATGCCT
TTCTCCACGCTTGTTGCTAACTCTTTCTTTGCCTTTTCCAAACTCGCCCT
CTCATAATCACTTAAGGGACCTAGAGGAAGAAATTCCTCAACTCCGTCAC
GGCCAAGTC
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