GH720661

EST Overview


Name	GH720661
Unique Name	GH720661
Type	EST
Organism	Pisum sativum (pea)
Sequence length	400

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Libraries


Library Name	Type
LIBEST_024272 BACI	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720661 vs. TrEMBL
Match: B9SWB7_RICCO (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, putative OS=Ricinus communis GN=RCOM_0154370 PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 4.225e-47
Identity = 90/126 (71.43%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYEELEILK +DATM+LHDLP TED++ RVHAARRLFE+LS+NSL+FPVIHHI FPNGIHRDDLVIGAG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  519 LVVSVRGDEPYEELEILKQIDATMILHDLPNTEDKIGRVHAARRLFEFLSDNSLNFPVIHHINFPNGIHRDDLVIGAGANAGALLVDGLGDGILLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 644

[Close]

BLAST of GH720661 vs. TrEMBL
Match: A9ZN14_HEVBR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Hevea brasiliensis GN=HbHDS PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 7.206e-47
Identity = 88/126 (69.84%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +    RGDEPYEELEILK +DATM+LHDLP+TED++ RVHAARRLFE+LS+N+L+FPVIHHIQFPN IHRDDLVIGAG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSARGDEPYEELEILKDIDATMILHDLPFTEDKIGRVHAARRLFEFLSDNALNFPVIHHIQFPNAIHRDDLVIGAGTNAGALLVDGLGDGILLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: D7SKY1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025182001 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.576e-46
Identity = 88/126 (69.84%), Postives = 104/126 (82.54%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYE+LEILK VDATM+LHDLP TE+++SRVH+ARRLFEYLS+N+L+FPVIHHIQFP GIHRDDLVIGAG++               EA ++DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEDLEILKEVDATMILHDLPLTEEKISRVHSARRLFEYLSDNALNFPVIHHIQFPKGIHRDDLVIGAGTNAGALLVDGLGDGLLLEAPEQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: A5BBY6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005654 PE=3 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.576e-46
Identity = 88/126 (69.84%), Postives = 104/126 (82.54%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYE+LEILK VDATM+LHDLP TE+++SRVH+ARRLFEYLS+N+L+FPVIHHIQFP GIHRDDLVIGAG++               EA ++DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEDLEILKEVDATMILHDLPLTEEKISRVHSARRLFEYLSDNALNFPVIHHIQFPKGIHRDDLVIGAGTNAGALLVDGLGDGLLLEAPEQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q9FF59_ARATH (GcpE protein OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  492 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 617

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8RXG8_ARATH (At5g60600 OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8LPQ4_ARATH (AT5g60600/mup24_10 OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8GZR7_ARATH (GcpE OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  516 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 641

[Close]

BLAST of GH720661 vs. TrEMBL
Match: B3H725_ARATH (Uncharacterized protein At5g60600.3 OS=Arabidopsis thaliana GN=At5g60600 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q84XR5_CATRO (GCPE protein OS=Catharanthus roseus PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 3.954e-45
Identity = 87/126 (69.05%), Postives = 101/126 (80.16%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYEEL+ILK VD TM+LH+LP+TE+++ RVHAARRLFEYLSENSL+FPVIHH+QF N IHRDDLVIGAGS                EA D+DF+F+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEELDILKNVDVTMILHNLPHTEEKIGRVHAARRLFEYLSENSLNFPVIHHMQFANAIHRDDLVIGAGSDAGALLVDGLGDGVMLEAHDQDFEFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.3 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363019 FORWARD LENGTH=717)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.2 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=740)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  516 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 641

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.1 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=741)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

The following BLAST results are available for this feature:

BLAST of GH720661 vs. TrEMBL
BLAST of GH720661 vs. TAIR peptide

BLAST of GH720661 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SWB7_RICCO	4.225e-47	71.43	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
A9ZN14_HEVBR	7.206e-47	69.84	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
D7SKY1_VITVI	3.576e-46	69.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BBY6_VITVI	3.576e-46	69.84	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q9FF59_ARATH	7.967e-46	69.05	GcpE protein OS=Arabidopsis thaliana PE=3 SV=1	[more]
Q8RXG8_ARATH	7.967e-46	69.05	At5g60600 OS=Arabidopsis thaliana GN=At5g60600 PE=...	[more]
Q8LPQ4_ARATH	7.967e-46	69.05	AT5g60600/mup24_10 OS=Arabidopsis thaliana GN=At5g...	[more]
Q8GZR7_ARATH	7.967e-46	69.05	GcpE OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=...	[more]
B3H725_ARATH	7.967e-46	69.05	Uncharacterized protein At5g60600.3 OS=Arabidopsis...	[more]
Q84XR5_CATRO	3.954e-45	69.05	GCPE protein OS=Catharanthus roseus PE=2 SV=1	[more]

BLAST of GH720661 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G60600.3	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.2	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.1	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 83..97 score
None	No IPR available	SEG	seg	seg	coord: 33..51 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720661 ID=GH720661; Name=GH720661; organism=Pisum sativum; type=EST; length=400bp
TAGCGTGGTCGCGGCCGAGGTACGTGGCGACGAGCCTTATGAAGAACTGG
AAATTCTCAAAGGTGTTGATGCAACTATGGTTCTCCATGACTTGCCTTAT
ACTGAAGACAGAGTTAGTAGAGTGCACGCAGCAAGGCGGTTATTCGAGTA
TCTATCAGAAAACTCGCTAGACTTTCCTGTCATTCACCACATTCAGTTTC
CAAATGGAATTCACAGGGATGACCTAGTGATTGGTGCTGGCTCTCATGCT
GGAGCCCTGCTGGTTGACGGGCTTGGAGACGGTCTTCTTTTAGAAGCTTC
AGACAAAGATTTTGACTTCATTAGAAATACATCTTTCAATTTGCTGCAAG
GATGCAGAATGAGAAATACAAAAACAGAGTACCTGCCCGGGCGGCCGCTC

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