GT627855

EST Overview


Name	GT627855
Unique Name	GT627855
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	786

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT627855 vs. TrEMBL
Match: Q9FVA2_PEA (Glutathione synthetase OS=Pisum sativum GN=gshs PE=2 SV=1)

HSP 1 Score: 416.772 bits (1070), Expect = 1.068e-114
Identity = 209/222 (94.14%), Postives = 215/222 (96.85%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H  DQ LLD+IAYDALVWASLHGL+MGDKSS+KSGTVPGVGLVHAPFAL PVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKM+H+NKKE+IRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAE VPVNNA  QNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV
Sbjct:   85 HRFDQQLLDSIAYDALVWASLHGLVMGDKSSKKSGTVPGVGLVHAPFALLPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMLHVNKKEEIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAENVPVNNAVIQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 306

[Close]

BLAST of GT627855 vs. TrEMBL
Match: A2Q600_MEDTR (Eukaryotic glutathione synthase; Eukaryotic glutathione synthase, ATP-binding OS=Medicago truncatula GN=MtrDRAFT_AC172101g16v1 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 4.499e-105
Identity = 192/222 (86.49%), Postives = 204/222 (91.89%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H IDQ LLDNIAYDALVWASLHGLLMGDKS QKSG VPGVGL+HAPF+LFP S PESK+KQACDLAPIFNELVDRVSLD  FLQESLSRTK+VDEFTSRLLDIHSKM+ LNKKE+IR GLHRSDYMLDEQTKSLLQIELNTISSSFAGF +LVT+LHRYIL+ HGKLLGLD+EKVP NNA +QNAE LAKAW EYNNP+AVIM VVQAEERNMYDQH LSAV
Sbjct:   89 HNIDQQLLDNIAYDALVWASLHGLLMGDKSYQKSGMVPGVGLMHAPFSLFPTSFPESKWKQACDLAPIFNELVDRVSLDGKFLQESLSRTKQVDEFTSRLLDIHSKMLQLNKKEEIRFGLHRSDYMLDEQTKSLLQIELNTISSSFAGFSTLVTQLHRYILTCHGKLLGLDSEKVPANNAVSQNAEALAKAWSEYNNPRAVIMTVVQAEERNMYDQHFLSAV 310

[Close]

BLAST of GT627855 vs. TrEMBL
Match: Q94GA0_MEDTR (Glutathione synthetase GSHS1 OS=Medicago truncatula GN=gshs1 PE=4 SV=1)

HSP 1 Score: 382.874 bits (982), Expect = 1.710e-104
Identity = 191/222 (86.04%), Postives = 203/222 (91.44%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H IDQ LLDNIAYDALVWASLHGLLMGDKS QKSG VPGVGL+HAPF+LFP S PESK+KQACDLAPIFNELVDRVSLD  FLQESL RTK+VDEFTSRLLDIHSKM+ LNKKE+IR GLHRSDYMLDEQTKSLLQIELNTISSSFAGF +LVT+LHRYIL+ HGKLLGLD+EKVP NNA +QNAE LAKAW EYNNP+AVIM VVQAEERNMYDQH LSAV
Sbjct:   89 HNIDQQLLDNIAYDALVWASLHGLLMGDKSYQKSGMVPGVGLMHAPFSLFPTSFPESKWKQACDLAPIFNELVDRVSLDGKFLQESLPRTKQVDEFTSRLLDIHSKMLQLNKKEEIRFGLHRSDYMLDEQTKSLLQIELNTISSSFAGFSTLVTQLHRYILTCHGKLLGLDSEKVPANNAVSQNAEALAKAWSEYNNPRAVIMTVVQAEERNMYDQHFLSAV 310

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BLAST of GT627855 vs. TrEMBL
Match: Q93XE5_LOTJA (Glutathione synthetase OS=Lotus japonicus PE=2 SV=2)

HSP 1 Score: 369.007 bits (946), Expect = 2.555e-100
Identity = 186/219 (84.93%), Postives = 201/219 (91.78%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            DQ LL  IA DALVWASLHGL+MGDKS Q+SGTVPGVGLVHAPFALFP   PES+++QACDLAPIFNELVDRVSLD  FLQESLSRTK+VDEFTSRLLDIHSKM+ +NKKE+IRLGLHRSDYMLDE+TKSLLQIELNTISSSFAG GSLVTELHRYILSR+GKLLGLD+ KVP NNAATQ AEGLA+AW EYN+P+AVIM VVQAEERNMYDQH LSAV
Sbjct:   86 DQQLLQKIADDALVWASLHGLVMGDKSVQRSGTVPGVGLVHAPFALFPTLFPESQWRQACDLAPIFNELVDRVSLDGKFLQESLSRTKEVDEFTSRLLDIHSKMLAINKKEEIRLGLHRSDYMLDEKTKSLLQIELNTISSSFAGLGSLVTELHRYILSRYGKLLGLDSGKVPANNAATQFAEGLARAWTEYNDPRAVIMIVVQAEERNMYDQHFLSAV 304

[Close]

BLAST of GT627855 vs. TrEMBL
Match: Q6XPU3_LOTJA (Homoglutathione synthetase OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 5.903e-89
Identity = 162/222 (72.97%), Postives = 191/222 (86.04%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H ID  LL+N+ YDALV+A+L+GLL+GDKS Q+SG VPGVGLVH PF+L P  LPES +KQAC+LAP+FNELVDRVSLD  FLQESLSRTKK DEFTSRLLDIHSKM+ +NKKE+IR+GLHRSDYMLDE+TK LLQIE+NTIS+SF+G G + TELHR ILS +GKLLGLD+ +VP N+A  + AE LAKAW+EYNNP+AVIM +VQ EE NMYDQH +SAV
Sbjct:   78 HQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLVHLPFSLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDIHSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSHYGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAV 299

[Close]

BLAST of GT627855 vs. TrEMBL
Match: Q9XEB8_MEDTR (Homoglutathione synthetase GSHS2 OS=Medicago truncatula GN=gshs2 PE=2 SV=2)

HSP 1 Score: 327.02 bits (837), Expect = 1.113e-87
Identity = 159/222 (71.62%), Postives = 194/222 (87.39%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H ID  +L+NI YDALV+A+L+GLL+GDKS ++SG VPGVGLVH PF+L P  LPE+ +KQACDL+P+FNELVDRVSLD  FLQ+SLS+TKK DEFTSRLLDIH+KM+ +NKKE+IR+GL RSDYMLDE+TKSLLQIE+NTIS+SF+G G ++TELHR ILS +GKLLGLD+E+VPVN A ++ AE LAKAW EYNNP+AVI+ VVQ EERNMYDQH +SA+
Sbjct:   79 HQIDDKVLENIVYDALVFATLNGLLVGDKSVERSGRVPGVGLVHLPFSLLPPPLPETHWKQACDLSPLFNELVDRVSLDGKFLQQSLSKTKKADEFTSRLLDIHAKMLEMNKKEEIRMGLFRSDYMLDEKTKSLLQIEMNTISTSFSGVGVVMTELHRNILSHYGKLLGLDSERVPVNTADSRYAEALAKAWKEYNNPRAVIIVVVQGEERNMYDQHFVSAI 300

[Close]

BLAST of GT627855 vs. TrEMBL
Match: A2Q601_MEDTR (Eukaryotic glutathione synthase; Eukaryotic glutathione synthase, ATP-binding OS=Medicago truncatula GN=MtrDRAFT_AC172101g15v1 PE=4 SV=1)

HSP 1 Score: 327.02 bits (837), Expect = 1.113e-87
Identity = 159/222 (71.62%), Postives = 194/222 (87.39%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H ID  +L+NI YDALV+A+L+GLL+GDKS ++SG VPGVGLVH PF+L P  LPE+ +KQACDL+P+FNELVDRVSLD  FLQ+SLS+TKK DEFTSRLLDIH+KM+ +NKKE+IR+GL RSDYMLDE+TKSLLQIE+NTIS+SF+G G ++TELHR ILS +GKLLGLD+E+VPVN A ++ AE LAKAW EYNNP+AVI+ VVQ EERNMYDQH +SA+
Sbjct:   35 HQIDDKVLENIVYDALVFATLNGLLVGDKSVERSGRVPGVGLVHLPFSLLPPPLPETHWKQACDLSPLFNELVDRVSLDGKFLQQSLSKTKKADEFTSRLLDIHAKMLEMNKKEEIRMGLFRSDYMLDEKTKSLLQIEMNTISTSFSGVGVVMTELHRNILSHYGKLLGLDSERVPVNTADSRYAEALAKAWKEYNNPRAVIIVVVQGEERNMYDQHFVSAI 256

[Close]

BLAST of GT627855 vs. TrEMBL
Match: Q9FV27_PEA (Putative homoglutathione synthetase OS=Pisum sativum GN=hgshs PE=2 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 1.608e-86
Identity = 157/222 (70.72%), Postives = 191/222 (86.04%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H ID+ ++  I YDALV+ +L+GLL+GDKS Q+SG VPGVGLVH PF+L P   P++ + QAC+LAP+FNELVDRVSLDANFLQ+SLSRTKKVDEFTSRLLDIHSKM+ +NKKE+IRLGL RSDYM DE+T+SLLQIE+NTIS+SF+G G ++T+LHR ILSR+GK LGLD+E+VP N A ++ AE LAKAW EYNNP AVI+ VVQAEERNMYDQH +SA+
Sbjct:   28 HEIDEKVVQQIVYDALVFTTLNGLLVGDKSVQRSGRVPGVGLVHLPFSLLPPPFPQTHWNQACELAPLFNELVDRVSLDANFLQQSLSRTKKVDEFTSRLLDIHSKMLEINKKEEIRLGLFRSDYMFDEKTQSLLQIEMNTISTSFSGVGCIITQLHRNILSRYGKSLGLDSERVPTNTADSKYAEALAKAWSEYNNPSAVIIVVVQAEERNMYDQHFVSAI 249

[Close]

BLAST of GT627855 vs. TrEMBL
Match: D7TV25_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_30.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00022056001 PE=4 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 3.032e-85
Identity = 155/210 (73.81%), Postives = 184/210 (87.62%), Query Frame = 2

Query:  149 IAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLS 778
            + YDALVW+SLHGL++GD++ +++GTVPGVG+VHAPFAL P+S PES ++QAC+L PIFNELVDRVSLD  FLQE+LSRTKKVD FTSRLLDIHSKM  +NKKE+IRLGLHRSDYMLDEQTK LLQIELNTISSSF G   LVTELHR +L+ +GK LGLD++++P N+A TQ AE LA+AW EYNNP+A++MFVVQ EERNMYDQH LS
Sbjct:    1 MVYDALVWSSLHGLVVGDRTVKRAGTVPGVGMVHAPFALLPMSFPESHWQQACELTPIFNELVDRVSLDWKFLQEALSRTKKVDSFTSRLLDIHSKMQEMNKKEEIRLGLHRSDYMLDEQTKLLLQIELNTISSSFPGLSCLVTELHRSLLNHYGKHLGLDSKRIPGNSAVTQFAEALAQAWKEYNNPRALVMFVVQTEERNMYDQHWLS 210

[Close]

BLAST of GT627855 vs. TrEMBL
Match: C9VXR2_VIGUN (Homoglutathione synthetase OS=Vigna unguiculata PE=2 SV=1)

HSP 1 Score: 318.161 bits (814), Expect = 5.171e-85
Identity = 156/222 (70.27%), Postives = 185/222 (83.33%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            H+IDQ LL+NI YDALVW++L+ LL+GDKS Q+SGTVPGVGLVH P  L P   PES +KQAC+LAPIFNELVDRVSLD  FLQESLSRTK  DEFTSRLLDIHSKM+ +NKKEDIRL + RSDYM+DE+TKSLLQIE+NTIS+SFA  G+LVT LH+ ++S++G +LGLD  +VP NNA  Q AE LAK W EYNNP+A I+ VVQ EERNMY+QH +SA+
Sbjct:   29 HSIDQKLLENIVYDALVWSTLNCLLVGDKSVQRSGTVPGVGLVHLPLCLLPGPFPESHWKQACELAPIFNELVDRVSLDGKFLQESLSRTKNADEFTSRLLDIHSKMLQINKKEDIRLAIVRSDYMVDEKTKSLLQIEMNTISTSFALMGTLVTGLHKSLISQYGNILGLDPNRVPANNAVDQAAEALAKGWSEYNNPRAAILVVVQVEERNMYEQHYISAL 250

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_BRAJU (Glutathione synthetase, chloroplastic OS=Brassica juncea GN=GSH2 PE=2 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 3.143e-84
Identity = 147/219 (67.12%), Postives = 183/219 (83.56%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            D   +  + YDALVW+SLHGL++GDK+ Q+SGTVPGVG++HAP AL P   PES + QAC++APIFNELVDR+SLD  F+Q+SLSRT+K D FTSRLL+IHSKM+  NK+E+IRLGLHRSDYMLDE+TKSLLQIE+NTIS SF GFG LVTELH+ +L  HG  LGLD+++VP N + +Q A+ +AKAW+EYNNP+AV+M VVQ +ERNMYDQH LS+V
Sbjct:   66 DDGFVQKLVYDALVWSSLHGLVVGDKTHQRSGTVPGVGMMHAPIALLPTPFPESYWNQACEVAPIFNELVDRISLDGKFIQDSLSRTRKADVFTSRLLEIHSKMLESNKREEIRLGLHRSDYMLDEETKSLLQIEMNTISCSFPGFGRLVTELHQSLLRSHGDHLGLDSKRVPRNGSTSQFADAMAKAWLEYNNPRAVVMVVVQPDERNMYDQHWLSSV 284

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_ARATH (Glutathione synthetase, chloroplastic OS=Arabidopsis thaliana GN=GSH2 PE=2 SV=3)

HSP 1 Score: 308.145 bits (788), Expect = 3.475e-83
Identity = 144/220 (65.45%), Postives = 181/220 (82.27%), Query Frame = 2

Query:  125 IDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            +D   +  + YDALVW+SLHGL++GDKS QKSG VPGVGL+HAP AL P + PE+ +KQAC++ P+FNEL+DRVSLD  FLQ+SLSRTKKVD FTSRLLDIHSKM+  NKKEDIRLGLHR DYMLDE+T SLLQIE+NTIS SF G   LV++LH+ +L  +G  +G+D+E+VP+N +  Q A+ LAKAW+EY+NP+AV+M +VQ EERNMYDQH LS++
Sbjct:   74 LDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPIALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGKFLQDSLSRTKKVDVFTSRLLDIHSKMLERNKKEDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVSQLHQSLLRSYGDQIGIDSERVPINTSTIQFADALAKAWLEYSNPRAVVMVIVQPEERNMYDQHLLSSI 293

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_SOLLC (Glutathione synthetase, chloroplastic OS=Solanum lycopersicum GN=GSH2 PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.118e-81
Identity = 149/221 (67.42%), Postives = 179/221 (81.00%), Query Frame = 2

Query:  119 HTIDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSA 781
            H ID  L+  +A DALVW  L GLL+GD++S++SGT+PGV +VHAP AL P+S PES +KQAC++APIFNELVDRVS D  FLQ+SLSRT+K D FTSRLL+IHSKM+ +NK E+IRLGLHRSDYMLDEQTK LLQIELNTISSSF G   LV+ELHR +L ++ + +  D  ++P NNA  Q AE LAKAW EY +P+AVI+FVVQAEERNMYDQH LSA
Sbjct:   79 HDIDSKLVQKLANDALVWCPLRGLLVGDRNSERSGTIPGVDMVHAPVALIPMSFPESHWKQACEVAPIFNELVDRVSQDGEFLQQSLSRTRKADPFTSRLLEIHSKMLEINKLEEIRLGLHRSDYMLDEQTKLLLQIELNTISSSFPGLSCLVSELHRSLLQQYREDIASDPNRIPANNAVNQFAEALAKAWNEYGDPRAVIIFVVQAEERNMYDQHWLSA 299

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_HUMAN (Glutathione synthetase OS=Homo sapiens GN=GSS PE=1 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 4.307e-33
Identity = 84/219 (38.36%), Postives = 130/219 (59.36%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLD---EQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSL 775
            D+  L+ +A  A+  A   G+L+       S  V    + +APF LFP  +P +  +QA  +   FN LVD VS +A FL+++LS T K D+FT+RL DIH +++     + + LGL+RSDYM     + + +L QIE+NTIS+SF G  S    +HR++LS   K    +A K+  NN +   A G+AKAW  Y +P A+++ + Q +ERN++DQ ++
Sbjct:   11 DKQQLEELARQAVDRALAEGVLLRTSQEPTSSEV----VSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKT--KEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAI 223

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_RAT (Glutathione synthetase OS=Rattus norvegicus GN=Gss PE=1 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 9.595e-33
Identity = 82/219 (37.44%), Postives = 131/219 (59.82%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLD---EQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSL 775
            D+  L+ +A  A+  A   G+L+    +  S  V    + +APF LFP  +P +  +QA  +   FN LVD VS ++ FL+++LS T K DE+T+RL DI+ +++     + + LGL+RSDYM     + +K+L QIE+NTIS+SF G  S    +HR++L+   K    +A K+  NN +   A G+AKAW  Y +  AV++ + Q +ERN++DQ ++
Sbjct:   11 DEKQLEELAQQAIDRALAEGVLLRSAKNPSSSDV----VTYAPFTLFPSPVPSTLLEQAYAVQMDFNILVDAVSQNSAFLEQTLSSTIKKDEYTARLFDIYKQVLKEGIAQTVFLGLNRSDYMFQCSADGSKALKQIEINTISASFGGLASRTPAVHRHVLNVLNKT--NEASKILSNNPSKGLALGIAKAWELYGSANAVVLLIAQEKERNIFDQRAI 223

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_BOVIN (Glutathione synthetase OS=Bos taurus GN=GSS PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.253e-32
Identity = 83/219 (37.90%), Postives = 129/219 (58.90%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLD---EQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSL 775
            D+  L+ +A  A+  A   G+L+    +  S  V    + +APF LFP  +P +  +QA  +   FN LVD VS +A FL+++LS T K D FT+RL DIH +++     + + LGL+RSDYM     + + +L QIE+NT+S+SF G  S    +HR++LS  GK    +A K+  NN +   A G+AKAW  Y +  A ++ + Q +ERN++DQ ++
Sbjct:   11 DEQQLEELARQAVDRALAEGVLLRTSQAPSSSHV----VSYAPFTLFPSPVPSALLEQAYAVQADFNLLVDAVSQNAVFLEQTLSSTIKRDSFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQCNPDGSAALKQIEINTVSASFGGLASRTPAVHRHVLSVLGKT--KEAAKILSNNPSKGLAMGIAKAWELYGSANAQVLLIAQEKERNIFDQRAI 223

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_XENLA (Glutathione synthetase OS=Xenopus laevis GN=gss PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.138e-32
Identity = 85/221 (38.46%), Postives = 129/221 (58.37%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYML---DEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSA 781
            D  LL+ +A  A+  A L G+LM  K S  S  V    +  APFAL P  +P++ F+QA  +   FN LVDR+S D +FL++ LS T KVD+F  RL  IH ++   +  +++ LG++RSDYM    D+ T +L QIE+NTI++SF G  S    +H+++L    K    ++  +  N+A      G+A AW  Y +  A +MF+V+ E+RN+ DQ  + A
Sbjct:   11 DTKLLEELAPIAIDAALLQGVLMRTKESPNSSDV----VSFAPFALLPSPVPKALFEQAKCVQEDFNTLVDRISQDTSFLEQVLSSTIKVDDFIRRLFAIHKQVQQEDCTQEVFLGINRSDYMFDCRDDGTPALKQIEINTIAASFGGLASRTPAVHQHVLKFLRK--SEESSSILTNDAVEGIGWGIAHAWALYGSVDATVMFLVENEQRNILDQRFIEA 225

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_MOUSE (Glutathione synthetase OS=Mus musculus GN=Gss PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 8.123e-32
Identity = 81/219 (36.99%), Postives = 129/219 (58.90%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLD---EQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSL 775
            D+  L+ +A  A+  A   G+L+       S  V    + +APF LFP  +P +  +QA  +   FN LVD VS +  FL+++LS T K D++T+RL DI+ +++     + + LGL+RSDYM     + +K+L QIE+NTIS+SF G  S    +HR++L+   K    +A K+  NN +   A G+AKAW  Y +  AV++ + Q +ERN++DQ ++
Sbjct:   11 DEKQLEELAKQAIDRALAEGVLLRSAQHPSSSDV----VTYAPFTLFPSPVPSALLEQAYAVQMDFNILVDAVSQNPAFLEQTLSSTIKKDDYTARLFDIYKQVLKEGIAQTVFLGLNRSDYMFQCGADGSKALKQIEINTISASFGGLASRTPAVHRHVLNVLNKT--KEASKILSNNPSKGLALGIAKAWELYGSANAVVLLIAQEKERNIFDQRAV 223

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_MACFA (Glutathione synthetase OS=Macaca fascicularis GN=GSS PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.363e-31
Identity = 81/219 (36.99%), Postives = 128/219 (58.45%), Query Frame = 2

Query:  128 DQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQT---KSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSL 775
            ++  L+ +A  A+  A   G+L+       S  V    + +APF LFP  +P +  +QA  +   FN LVD V+ +A FL+++LS T + D+FT+RL DIH +++     + + LGL+RSDYM    T    +L QIE+NTIS+SF G  S    +HR++LS   K    +A K+  NN +   A G+AKAW  Y +  A+++ + Q +ERN++DQ ++
Sbjct:   11 NEQQLEELARQAVDRALAEGVLLRTSQEPTSSEV----VSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVNQNAAFLEQTLSSTIEQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSTDGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKT--KEAGKILSNNPSKGLALGIAKAWELYGSTNALVLLIAQEKERNIFDQRAI 223

[Close]

BLAST of GT627855 vs. SwissProt
Match: GSHB_DICDI (Glutathione synthetase OS=Dictyostelium discoideum GN=gshB PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 8.406e-29
Identity = 76/218 (34.86%), Postives = 123/218 (56.42%), Query Frame = 2

Query:  140 LDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYM----LDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHG-KLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPK-AVIMFVVQAEERNMYDQHSL 775
            L+++    + WA  +GL+M  K +++      V + H PF+L+P  + +  F +AC LA  +N LV  +S D +FLQ +L      D+FT  LL+I  K++    K+ I LG+ RSDYM    ++ + + + Q+ELNTISSS A   + V  LH+Y++ R+     G D    P N +  + ++ +A A   YN  K +V++ ++Q  ERN+YDQ  L
Sbjct:    5 LNDLKEQGIDWAFANGLIMIKKPTEEEAKNNVVNVTHVPFSLYPSKMNKKLFNEACKLAEDYNLLVHNISKDYDFLQNTLKDV--FDDFTQMLLNIQRKVVKEGIKQKISLGIFRSDYMFHNKIEGEEERIYQVELNTISSSLAVVSNRVFNLHKYLIGRNDLNDNGYDLLNHPTNQSDKEISDSIALAHKLYNKEKSSVVLMIIQEGERNIYDQKGL 220

[Close]

BLAST of GT627855 vs. TAIR peptide
Match: AT5G27380.1 (| Symbols: GSH2, GSHB | glutathione synthetase 2 | chr5:9668211-9670912 REVERSE LENGTH=539)

HSP 1 Score: 308.145 bits (788), Expect = 3.459e-84
Identity = 144/220 (65.45%), Postives = 181/220 (82.27%), Query Frame = 2

Query:  125 IDQHLLDNIAYDALVWASLHGLLMGDKSSQKSGTVPGVGLVHAPFALFPVSLPESKFKQACDLAPIFNELVDRVSLDANFLQESLSRTKKVDEFTSRLLDIHSKMIHLNKKEDIRLGLHRSDYMLDEQTKSLLQIELNTISSSFAGFGSLVTELHRYILSRHGKLLGLDAEKVPVNNAATQNAEGLAKAWIEYNNPKAVIMFVVQAEERNMYDQHSLSAV 784
            +D   +  + YDALVW+SLHGL++GDKS QKSG VPGVGL+HAP AL P + PE+ +KQAC++ P+FNEL+DRVSLD  FLQ+SLSRTKKVD FTSRLLDIHSKM+  NKKEDIRLGLHR DYMLDE+T SLLQIE+NTIS SF G   LV++LH+ +L  +G  +G+D+E+VP+N +  Q A+ LAKAW+EY+NP+AV+M +VQ EERNMYDQH LS++
Sbjct:   74 LDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPIALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGKFLQDSLSRTKKVDVFTSRLLDIHSKMLERNKKEDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVSQLHQSLLRSYGDQIGIDSERVPINTSTIQFADALAKAWLEYSNPRAVVMVIVQPEERNMYDQHLLSSI 293

The following BLAST results are available for this feature:

BLAST of GT627855 vs. TrEMBL
BLAST of GT627855 vs. SwissProt
BLAST of GT627855 vs. TAIR peptide

BLAST of GT627855 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FVA2_PEA	1.068e-114	94.14	Glutathione synthetase OS=Pisum sativum GN=gshs PE...	[more]
A2Q600_MEDTR	4.499e-105	86.49	Eukaryotic glutathione synthase; Eukaryotic glutat...	[more]
Q94GA0_MEDTR	1.710e-104	86.04	Glutathione synthetase GSHS1 OS=Medicago truncatul...	[more]
Q93XE5_LOTJA	2.555e-100	84.93	Glutathione synthetase OS=Lotus japonicus PE=2 SV=...	[more]
Q6XPU3_LOTJA	5.903e-89	72.97	Homoglutathione synthetase OS=Lotus japonicus PE=2...	[more]
Q9XEB8_MEDTR	1.113e-87	71.62	Homoglutathione synthetase GSHS2 OS=Medicago trunc...	[more]
A2Q601_MEDTR	1.113e-87	71.62	Eukaryotic glutathione synthase; Eukaryotic glutat...	[more]
Q9FV27_PEA	1.608e-86	70.72	Putative homoglutathione synthetase OS=Pisum sativ...	[more]
D7TV25_VITVI	3.032e-85	73.81	Whole genome shotgun sequence of line PN40024, sca...	[more]
C9VXR2_VIGUN	5.171e-85	70.27	Homoglutathione synthetase OS=Vigna unguiculata PE...	[more]

BLAST of GT627855 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GSHB_BRAJU	3.143e-84	67.12	Glutathione synthetase, chloroplastic OS=Brassica ...	[more]
GSHB_ARATH	3.475e-83	65.45	Glutathione synthetase, chloroplastic OS=Arabidops...	[more]
GSHB_SOLLC	1.118e-81	67.42	Glutathione synthetase, chloroplastic OS=Solanum l...	[more]
GSHB_HUMAN	4.307e-33	38.36	Glutathione synthetase OS=Homo sapiens GN=GSS PE=1...	[more]
GSHB_RAT	9.595e-33	37.44	Glutathione synthetase OS=Rattus norvegicus GN=Gss...	[more]
GSHB_BOVIN	1.253e-32	37.90	Glutathione synthetase OS=Bos taurus GN=GSS PE=2 S...	[more]
GSHB_XENLA	2.138e-32	38.46	Glutathione synthetase OS=Xenopus laevis GN=gss PE...	[more]
GSHB_MOUSE	8.123e-32	36.99	Glutathione synthetase OS=Mus musculus GN=Gss PE=2...	[more]
GSHB_MACFA	2.363e-31	36.99	Glutathione synthetase OS=Macaca fascicularis GN=G...	[more]
GSHB_DICDI	8.406e-29	34.86	Glutathione synthetase OS=Dictyostelium discoideum...	[more]

BLAST of GT627855 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G27380.1	3.459e-84	65.45	\| Symbols: GSH2, GSHB \| glutathione synthetase 2 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004887	Glutathione synthase, substrate-binding, eukaryotic	GENE3D	3.40.50.1760	no description	coord: 217..259 score: 1.8
IPR005615	Glutathione synthase, eukaryotic	PANTHER	PTHR11130	GLUTATHIONE SYNTHETASE	coord: 27..260 score: 2.6
IPR005615	Glutathione synthase, eukaryotic	PFAM	PF03917	GSH_synth_ATP	coord: 38..238 score: 6.3
IPR014042	Glutathione synthase, alpha-helical, eukaryotic	GENE3D	1.10.1080.10	no description	coord: 120..187 score: 4
IPR014049	Glutathione synthase, N-terminal, eukaryotic	GENE3D	3.30.1490.80	no description	coord: 36..119 score: 3.2
None	No IPR available	SEG	seg	seg	coord: 2..17 score: NAcoord: 25..37 score
None	No IPR available	SUPERFAMILY	56059	Glutathione synthetase ATP-binding domain-like	coord: 35..235 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 1..19 score
None	No IPR available	SEG	seg	seg	coord: 4..17 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627855 ID=GT627855; Name=GT627855; organism=Lens culinaris; type=EST; length=786bp
CTTCCCCAAACCTCTACTCTCTCCTCACCATCACCACCTCACCATGCCAC
CTGTCGTTATTAACGGCAAACAAACCGTAAACGTCGACGATTCTTCTTCT
TCCGTTACCGTTAAATCTCACACCATCGATCAACACCTCCTCGATAACAT
CGCTTACGATGCTCTCGTTTGGGCTTCTCTTCATGGCCTCCTCATGGGCG
ATAAATCTTCCCAGAAATCGGGAACTGTTCCTGGTGTTGGATTGGTGCAT
GCTCCATTTGCGTTATTTCCCGTGTCATTGCCGGAAAGTAAATTTAAACA
AGCTTGTGATTTAGCACCTATATTCAATGAGCTTGTTGATCGTGTTAGTT
TGGATGCTAACTTTCTTCAGGAATCTCTCTCCAGGACTAAGAAAGTGGAC
GAATTTACCTCTAGGCTTTTAGATATTCATTCCAAGATGATACACCTCAA
CAAAAAAGAGGATATACGGTTGGGGTTGCATCGTTCGGATTATATGCTTG
ATGAACAGACGAAATCACTCTTGCAAATAGAGCTCAACACTATTTCATCT
TCATTTGCTGGTTTTGGTAGTCTTGTTACTGAGCTTCATAGGTACATACT
TTCCCGTCATGGAAAATTGCTTGGTCTGGATGCAGAAAAGGTTCCTGTCA
ATAACGCTGCCACTCAGAATGCAGAGGGCTTGGCTAAAGCTTGGATTGAG
TATAATAACCCCAAGGCTGTCATTATGTTTGTGGTTCAGGCTGAAGAGCG
AAACATGTATGATCAGCACTCTCTTTCTGCAGTTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004363	glutathione synthase activity
GO:0005524	ATP binding
GO:0016874	ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006750	glutathione biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR004887	GSH_synth_subst-bd
IPR005615	Glutathione_synthase
IPR014042	Glutathione_synthase_a-hlx
IPR014049	Glutathione_synthase_N_euk

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