GT627899

EST Overview
NameGT627899
Unique NameGT627899
TypeEST
OrganismLens culinaris (lentil)
Sequence length475
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Lens culinaris unigene v12010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Lens culinaris unigene v12011-01-06
Homology
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BLAST of GT627899 vs. TrEMBL
Match: B9SNW9_RICCO (1-aminocyclopropane-1-carboxylate deaminase, putative OS=Ricinus communis GN=RCOM_0582700 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.009e-34
Identity = 69/83 (83.13%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            KE+A ATGVVLDPVYSGKAAYAM+KD+ ENPKKWEGRKILF+HTGGLLGLYDKVDQ+ S V NW RMDV ESVPR  GTGKMF
Sbjct:  345 KEVATATGVVLDPVYSGKAAYAMMKDMAENPKKWEGRKILFVHTGGLLGLYDKVDQMSSLVKNWSRMDVDESVPRNAGTGKMF 427
[Close]
BLAST of GT627899 vs. TrEMBL
Match: B2MWN0_SOLLC (D-cysteine desulfhydrase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.598e-33
Identity = 62/83 (74.70%), Postives = 75/83 (90.36%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K++AE TGV+LDPVYSGKAAY M+KD+ ENP KWEGRKILFIHTGGLLGLYDK D++GS +G W++MD++ES+PRQDG GKMF
Sbjct:  343 KQVAETTGVILDPVYSGKAAYGMMKDMGENPTKWEGRKILFIHTGGLLGLYDKADEIGSLMGKWRKMDINESIPRQDGIGKMF 425
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BLAST of GT627899 vs. TrEMBL
Match: Q6ZHE5_ORYSJ (Os02g0773300 protein OS=Oryza sativa subsp. japonica GN=OJ1611_C08.26 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.684e-32
Identity = 64/83 (77.11%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K+IA ATG+VLDPVYSGKAAY M+KD+  NP KWEGRKILF+HTGGLLGLYDKVD+L S  G+W+RMD+ ESVPR+DGTGKMF
Sbjct:  303 KDIATATGIVLDPVYSGKAAYGMLKDMGANPAKWEGRKILFVHTGGLLGLYDKVDELSSLSGSWRRMDLEESVPRKDGTGKMF 385
[Close]
BLAST of GT627899 vs. TrEMBL
Match: B8AJJ5_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09120 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.684e-32
Identity = 64/83 (77.11%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K+IA ATG+VLDPVYSGKAAY M+KD+  NP KWEGRKILF+HTGGLLGLYDKVD+L S  G+W+RMD+ ESVPR+DGTGKMF
Sbjct:  303 KDIATATGIVLDPVYSGKAAYGMLKDMGANPAKWEGRKILFVHTGGLLGLYDKVDELSSLSGSWRRMDLEESVPRKDGTGKMF 385
[Close]
BLAST of GT627899 vs. TrEMBL
Match: A6N1I5_ORYSI (1-aminocyclopropane-1-carboxylate deaminase (Fragment) OS=Oryza sativa subsp. indica PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.684e-32
Identity = 64/83 (77.11%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K+IA ATG+VLDPVYSGKAAY M+KD+  NP KWEGRKILF+HTGGLLGLYDKVD+L S  G+W+RMD+ ESVPR+DGTGKMF
Sbjct:   74 KDIATATGIVLDPVYSGKAAYGMLKDMGANPAKWEGRKILFVHTGGLLGLYDKVDELSSLSGSWRRMDLEESVPRKDGTGKMF 156
[Close]
BLAST of GT627899 vs. TrEMBL
Match: B9I4W9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_569406 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 2.200e-32
Identity = 66/83 (79.52%), Postives = 73/83 (87.95%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            KEIA ATGVVLDPVYSGKAAY M+KD+ ENPK WEGRK+LFIHTGGLLGL+DKVDQ+ S V NW RM+V ESVPR+DG GKMF
Sbjct:  305 KEIATATGVVLDPVYSGKAAYGMMKDMAENPKNWEGRKVLFIHTGGLLGLFDKVDQMSSLVENWGRMEVQESVPRKDGIGKMF 387
[Close]
BLAST of GT627899 vs. TrEMBL
Match: D7KD10_ARALY (D-cysteine desulfhydrase OS=Arabidopsis lyrata subsp. lyrata GN=D-CDES PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 4.900e-32
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            KE+A +TGV+LDPVYSGKA Y ++ +I+++PK WEGRKILFIHTGGLLGLYDKVDQ+ S +GNW RMDVSESVPR+DG GKMF
Sbjct:  320 KEVASSTGVILDPVYSGKAVYGLINEISKDPKNWEGRKILFIHTGGLLGLYDKVDQMASLMGNWCRMDVSESVPRKDGVGKMF 402
[Close]
BLAST of GT627899 vs. TrEMBL
Match: Q9SX74_ARATH (F11A17.2 protein OS=Arabidopsis thaliana GN=F11A17.2 PE=4 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 6.400e-32
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K++A +TGV+LDPVYSGKAAY ++ +I ++PK WEGRKILFIHTGGLLGLYDKVDQ+ S +GNW RMDVSESVPR+DG GKMF
Sbjct:  332 KKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRKILFIHTGGLLGLYDKVDQMASLMGNWSRMDVSESVPRKDGVGKMF 414
[Close]
BLAST of GT627899 vs. TrEMBL
Match: Q8W4C7_ARATH (Putative uncharacterized protein At1g48420 OS=Arabidopsis thaliana GN=At1g48420/F11A17.2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 6.400e-32
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K++A +TGV+LDPVYSGKAAY ++ +I ++PK WEGRKILFIHTGGLLGLYDKVDQ+ S +GNW RMDVSESVPR+DG GKMF
Sbjct:  300 KKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRKILFIHTGGLLGLYDKVDQMASLMGNWSRMDVSESVPRKDGVGKMF 382
[Close]
BLAST of GT627899 vs. TrEMBL
Match: A9PHW2_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 6.400e-32
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            KEIA  TGVVLDPVYSGKAAY M+KD+ ENPK WEGRK+LFIHTGGLLGL+DKVDQ+ S V NW RM+V ESVPR+DG GKMF
Sbjct:  305 KEIATTTGVVLDPVYSGKAAYGMMKDMAENPKNWEGRKVLFIHTGGLLGLFDKVDQMSSLVENWGRMEVQESVPRKDGIGKMF 387
[Close]
BLAST of GT627899 vs. TAIR peptide
Match: AT1G48420.1 (| Symbols: D-CDES, ATACD1, ACD1 | D-cysteine desulfhydrase | chr1:17896767-17898803 REVERSE LENGTH=401)

HSP 1 Score: 140.198 bits (352), Expect = 5.148e-34
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 2 
Query:    2 KEIAEATGVVLDPVYSGKAAYAMVKDINENPKKWEGRKILFIHTGGLLGLYDKVDQLGSFVGNWQRMDVSESVPRQDGTGKMF 250
            K++A +TGV+LDPVYSGKAAY ++ +I ++PK WEGRKILFIHTGGLLGLYDKVDQ+ S +GNW RMDVSESVPR+DG GKMF
Sbjct:  319 KKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRKILFIHTGGLLGLYDKVDQMASLMGNWSRMDVSESVPRKDGVGKMF 401
The following BLAST results are available for this feature:
BLAST of GT627899 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9SNW9_RICCO4.009e-3483.131-aminocyclopropane-1-carboxylate deaminase, putat... [more]
B2MWN0_SOLLC2.598e-3374.70D-cysteine desulfhydrase OS=Solanum lycopersicum P... [more]
Q6ZHE5_ORYSJ1.684e-3277.11Os02g0773300 protein OS=Oryza sativa subsp. japoni... [more]
B8AJJ5_ORYSI1.684e-3277.11Putative uncharacterized protein OS=Oryza sativa s... [more]
A6N1I5_ORYSI1.684e-3277.111-aminocyclopropane-1-carboxylate deaminase (Fragm... [more]
B9I4W9_POPTR2.200e-3279.52Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7KD10_ARALY4.900e-3274.70D-cysteine desulfhydrase OS=Arabidopsis lyrata sub... [more]
Q9SX74_ARATH6.400e-3274.70F11A17.2 protein OS=Arabidopsis thaliana GN=F11A17... [more]
Q8W4C7_ARATH6.400e-3274.70Putative uncharacterized protein At1g48420 OS=Arab... [more]
A9PHW2_POPTR6.400e-3278.31Putative uncharacterized protein OS=Populus tricho... [more]
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BLAST of GT627899 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G48420.15.148e-3474.70| Symbols: D-CDES, ATACD1, ACD1 | D-cysteine desul... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001926Pyridoxal phosphate-dependent enzyme, beta subunitSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 1..60
score: 7
NoneNo IPR availableGENE3D3.40.50.1100no descriptioncoord: 1..57
score: 1.9
NoneNo IPR availablePANTHERPTHR10314SER/THR DEHYDRATASE, TRP SYNTHASEcoord: 1..83
score: 1.9
NoneNo IPR availablePANTHERPTHR10314:SF61-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASEcoord: 1..83
score: 1.9

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT627899 ID=GT627899; Name=GT627899; organism=Lens culinaris; type=EST; length=475bp
AAAAGAAATTGCTGAAGCTACAGGGGTTGTTCTTGACCCGGTATACAGTG
GTAAGGCTGCATATGCAATGGTGAAAGACATAAATGAAAATCCAAAGAAA
TGGGAAGGGAGGAAGATCCTATTCATACATACAGGCGGGCTCTTGGGATT
GTATGACAAGGTGGATCAGCTGGGTTCATTTGTAGGGAATTGGCAGCGAA
TGGATGTTAGTGAATCTGTTCCTAGGCAGGATGGAACAGGAAAGATGTTC
TAGATGTTTTTCAATTACATTTTATTTTATTTCACTGAATCCTCCAGTTC
ACATGTGTTACTATAAATAAGTGACTCGGTTATTTGATTTAGGGTTCAAC
TTTCGGTGAATATGTCTTGTGTTTTTTCTCTTTATCAAAGTTGGATCATG
GATGTAGAATAAGTTCTATGTCAAGTTGGATCATGAAGGTAGAATAAAAT
TAAATAAAATATATATTTTTGGGCA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR001926TrpB-like_PALP