GT623403

EST Overview
NameGT623403
Unique NameGT623403
TypeEST
OrganismLens culinaris (lentil)
Sequence length456
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Lens culinaris unigene v12010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Lens culinaris unigene v12011-01-06
Homology
[Close]
BLAST of GT623403 vs. TrEMBL
Match: B7FNC0_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.133e-28
Identity = 59/65 (90.77%), Postives = 65/65 (100.00%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAPDRKKFMEVIQGGGD+NADIE+FCT+FSPFLEENHKFLARFGLD++KAS
Sbjct:  138 HGWLASSSFTVAMKLAPDRKKFMEVIQGGGDINADIEQFCTSFSPFLEENHKFLARFGLDDMKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C6T3E3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.412e-25
Identity = 54/65 (83.08%), Postives = 61/65 (93.85%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTV MKLAPDRKKFM+VI G G+++ADIEKFCTTFSP LEENHKFLARFGLD++KAS
Sbjct:  138 HGWLASSSFTVVMKLAPDRKKFMDVIGGTGNISADIEKFCTTFSPLLEENHKFLARFGLDDMKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C6TBC2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.601e-25
Identity = 54/65 (83.08%), Postives = 59/65 (90.77%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAPDRKKFMEVIQG GD+ ADI+KFCT FSP  EENHKFLAR GLD++KAS
Sbjct:  138 HGWLASSSFTVAMKLAPDRKKFMEVIQGTGDIRADIQKFCTDFSPIFEENHKFLARCGLDDMKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C0PBG1_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 9.928e-25
Identity = 54/65 (83.08%), Postives = 60/65 (92.31%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAP+R+KFMEVI G GD+NADIEKFCTTFSPFL+ENH FLA  GLD+LKAS
Sbjct:   93 HGWLASSSFTVAMKLAPNREKFMEVISGTGDINADIEKFCTTFSPFLKENHNFLASVGLDDLKAS 157
[Close]
BLAST of GT623403 vs. TrEMBL
Match: B6T6M9_MAIZE (Pleckstrin homology domain containing, family A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 9.928e-25
Identity = 54/65 (83.08%), Postives = 60/65 (92.31%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAP+R+KFMEVI G GD+NADIEKFCTTFSPFL+ENH FLA  GLD+LKAS
Sbjct:  138 HGWLASSSFTVAMKLAPNREKFMEVISGTGDINADIEKFCTTFSPFLKENHNFLASVGLDDLKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: B4FX42_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=2)

HSP 1 Score: 116.316 bits (290), Expect = 9.928e-25
Identity = 54/65 (83.08%), Postives = 60/65 (92.31%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAP+R+KFMEVI G GD+NADIEKFCTTFSPFL+ENH FLA  GLD+LKAS
Sbjct:  138 HGWLASSSFTVAMKLAPNREKFMEVISGTGDINADIEKFCTTFSPFLKENHNFLASVGLDDLKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C5XXS7_SORBI (Putative uncharacterized protein Sb04g026420 OS=Sorghum bicolor GN=Sb04g026420 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.889e-24
Identity = 53/65 (81.54%), Postives = 59/65 (90.77%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAMKLAP+R KFMEVI G GD+NADIEKFCTTFSPFL+ENH FLA  GLD++KAS
Sbjct:  138 HGWLASSSFTVAMKLAPNRDKFMEVISGTGDINADIEKFCTTFSPFLKENHNFLASVGLDDMKAS 202
[Close]
BLAST of GT623403 vs. TrEMBL
Match: B9HSG5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_565782 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.773e-24
Identity = 53/65 (81.54%), Postives = 59/65 (90.77%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FT+AMKLAPDRKKFMEVI G GDV AD+E+FCTTF PFLEENHKFLA  GLD++KAS
Sbjct:  143 HGWLASSSFTIAMKLAPDRKKFMEVIAGTGDVAADMEEFCTTFPPFLEENHKFLASVGLDDMKAS 207
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C6SZA8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.927e-24
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKA 194
            HGWLASS+F+VAMKLAPDRKKFMEVIQG GD++ADI+KFCT FSP  EENHKFLAR GLD++KA
Sbjct:  138 HGWLASSSFSVAMKLAPDRKKFMEVIQGTGDISADIQKFCTDFSPIFEENHKFLARCGLDDMKA 201
[Close]
BLAST of GT623403 vs. TrEMBL
Match: C5Z4D9_SORBI (Putative uncharacterized protein Sb10g003870 OS=Sorghum bicolor GN=Sb10g003870 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.927e-24
Identity = 52/65 (80.00%), Postives = 60/65 (92.31%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASS+FTVAM+LAP+R KFMEVI G GD+NADIEKFCTTFSPFL+ENH+FLA  GLD++KAS
Sbjct:  138 HGWLASSSFTVAMRLAPNRDKFMEVISGTGDINADIEKFCTTFSPFLKENHEFLASVGLDDMKAS 202
[Close]
BLAST of GT623403 vs. TAIR peptide
Match: AT2G33470.2 (| Symbols: GLTP1 | glycolipid transfer protein 1 | chr2:14176599-14177950 REVERSE LENGTH=202)

HSP 1 Score: 103.605 bits (257), Expect = 4.869e-23
Identity = 44/65 (67.69%), Postives = 56/65 (86.15%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASSTF++A+KLAPDRKKFM+VI G G++ AD+E+FC  F PFL +NHKFLA  G+D++KAS
Sbjct:  138 HGWLASSTFSMALKLAPDRKKFMDVISGSGNIQADMERFCAEFGPFLHDNHKFLASVGMDDMKAS 202
[Close]
BLAST of GT623403 vs. TAIR peptide
Match: AT2G33470.1 (| Symbols: GLTP1, ATGLTP1 | glycolipid transfer protein 1 | chr2:14176599-14177950 REVERSE LENGTH=202)

HSP 1 Score: 103.605 bits (257), Expect = 4.869e-23
Identity = 44/65 (67.69%), Postives = 56/65 (86.15%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVIQGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKAS 197
            HGWLASSTF++A+KLAPDRKKFM+VI G G++ AD+E+FC  F PFL +NHKFLA  G+D++KAS
Sbjct:  138 HGWLASSTFSMALKLAPDRKKFMDVISGSGNIQADMERFCAEFGPFLHDNHKFLASVGMDDMKAS 202
[Close]
BLAST of GT623403 vs. TAIR peptide
Match: AT1G21360.1 (| Symbols: GLTP2 | glycolipid transfer protein 2 | chr1:7481365-7483237 FORWARD LENGTH=223)

HSP 1 Score: 51.6026 bits (122), Expect = 2.196e-7
Identity = 25/68 (36.76%), Postives = 39/68 (57.35%), Query Frame = 3 
Query:    3 HGWLASSTFTVAMKLAPDRKKFMEVI----QGGGDVNADIEKFCTTFSPFLEENHKFLARFGLDELKA 194
            HGW+AS+ F V +KL PD K FME I    +    +  DI+   +  +P L+E +  L ++GL  L++
Sbjct:  155 HGWIASAAFKVCLKLVPDNKTFMEAIGARDESYDTLREDIDTLSSLLTPILKEIYFVLEQYGLSRLRS 222
The following BLAST results are available for this feature:
BLAST of GT623403 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FNC0_MEDTR1.133e-2890.77Putative uncharacterized protein OS=Medicago trunc... [more]
C6T3E3_SOYBN3.412e-2583.08Putative uncharacterized protein OS=Glycine max PE... [more]
C6TBC2_SOYBN7.601e-2583.08Putative uncharacterized protein OS=Glycine max PE... [more]
C0PBG1_MAIZE9.928e-2583.08Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
B6T6M9_MAIZE9.928e-2583.08Pleckstrin homology domain containing, family A OS... [more]
B4FX42_MAIZE9.928e-2583.08Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
C5XXS7_SORBI2.889e-2481.54Putative uncharacterized protein Sb04g026420 OS=So... [more]
B9HSG5_POPTR3.773e-2481.54Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
C6SZA8_SOYBN4.927e-2481.25Putative uncharacterized protein OS=Glycine max PE... [more]
C5Z4D9_SORBI4.927e-2480.00Putative uncharacterized protein Sb10g003870 OS=So... [more]
back to top
BLAST of GT623403 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT2G33470.24.869e-2367.69| Symbols: GLTP1 | glycolipid transfer protein 1 |... [more]
AT2G33470.14.869e-2367.69| Symbols: GLTP1, ATGLTP1 | glycolipid transfer pr... [more]
AT1G21360.12.196e-736.76| Symbols: GLTP2 | glycolipid transfer protein 2 |... [more]
back to top
InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014830Glycolipid transfer protein domainGENE3D1.10.3520.10no descriptioncoord: 1..63
score: 2.6
IPR014830Glycolipid transfer protein domainPFAMPF08718GLTPcoord: 1..28
score: 5
IPR014830Glycolipid transfer protein domainSUPERFAMILY110004Glycolipid transfer protein, GLTPcoord: 1..63
score: 3.8

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT623403 ID=GT623403; Name=GT623403; organism=Lens culinaris; type=EST; length=456bp
GGCATGGATGGCTTGCTAGTTCAACTTTCACTGTAGCGATGAAGCTTGCT
CCTGATAGGAAGAAATTCATGGAGGTGATTCAGGGAGGTGGTGATGTTAA
TGCTGACATAGAGAAATTTTGTACCACCTTTTCTCCTTTCCTTGAAGAGA
ATCACAAGTTTCTGGCTCGTTTTGGCTTGGATGAATTGAAGGCTTCATGA
AGCATGGATTCAGTGTCTATTGATGAATGGCTCTTGCATGCTCAACCCTC
TTTTTTGGTTGTGTCTTTGTGTTTATGCTCCTTGTTTCTCTTTTGGCATC
CAAAAGAATAAATATGATAACAGAAAAATCAATGACTTACAGTCATTTTA
CTTCACATATGACTTGTTTTCTGCTTGGTGGTATGGAATGTTGAAACATA
TGCAACTTTGTTTGACTTGAAGTAAATTTAATGAACTATATAGTTTTGCA
TGTGGT
back to top
Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0017089glycolipid transfer activity
GO:0051861glycolipid binding
Vocabulary: Biological Process
TermDefinition
GO:0046836glycolipid transport
Vocabulary: INTERPRO
TermDefinition
IPR014830Glycolipid_transfer_prot_dom