GT627380

EST Overview


Name	GT627380
Unique Name	GT627380
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	804

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT627380 vs. TrEMBL
Match: C6TEZ0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 5.203e-64
Identity = 137/271 (50.55%), Postives = 166/271 (61.25%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQTDINQHTGTHQ--VSRLLEQGXXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVATNRSESRXXXXXXXXXXXXXXXXXXXXXXXSGSEIQTTDRNIQ--ISPLDP 803
            FYDCNICLD ARDPVLTCCGHLFCWPCFYQ+  VYS A+ECPVCKGEVTE+GI PIYGN +A G C+  +K AGLR+PPRP APRIES RQ+LI+QGASSS ++ +IRRFH+ IGG G + Q ++P N   DRNN  L +SR  +  ++ TG+ +  +S LL QG                  ERLV+DLESY H +   G    +T   N ++T S  A + S                           GSEIQTTD NIQ   S LDP
Sbjct:  120 FYDCNICLDRARDPVLTCCGHLFCWPCFYQVQIVYSNARECPVCKGEVTETGIFPIYGNSSADGSCESGLKGAGLRIPPRPAAPRIESFRQQLISQGASSS-VILNIRRFHHLIGGLGARVQSQNP-NAATDRNNGLLAQSRPPSSNDRGTGSSRTPISTLLVQGAASFSSLSSALNSAMDSAERLVEDLESYFHNHQTNGVNTSSTHNGNVAATDSTPAASTSPFSRNVDTTANI---------------GSEIQTTDNNIQTETSTLDP 373

[Close]

BLAST of GT627380 vs. TrEMBL
Match: C6TIN8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 4.404e-63
Identity = 139/272 (51.10%), Postives = 166/272 (61.03%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPT-NTRADRNNRTLVRSRTQTDINQHTGTHQ--VSRLLEQGXXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVATNRSESRXXXXXXXXXXXXXXXXXXXXXXXSGSEIQTTDRNIQ--ISPLDP 803
            FYDCNICLD ARDPVL CCGHLFCW CFYQ+  VYS A+ECPVCKGEVTE+GIIPIYGN +A G  +  +K AG+R+PPRP APRIES RQ+LI+QGA SSS++Q+I RFH+ IGG G + Q +S T N   DRNN  L +SR QT  ++ TG+ Q  +S LL QG                  ERLV+DLESYIH +   G    +T   N ++T S  A + S                           G EIQTTD NIQ   SPLDP
Sbjct:  120 FYDCNICLDRARDPVLACCGHLFCWQCFYQVQIVYSNARECPVCKGEVTETGIIPIYGNSSADGSRESGLKGAGMRIPPRPAAPRIESFRQQLISQGA-SSSVIQNIWRFHHLIGGLGARVQSQSQTPNAATDRNNGLLAQSRLQTGNDRGTGSSQTPISTLLVQGAASFSSLSSALNSAMDSAERLVEDLESYIHNHPTNGVNTNSTHDGNVAATDSTPAASTSH---------------FSRNVDTTADIGLEIQTTDSNIQTETSPLDP 375

[Close]

BLAST of GT627380 vs. TrEMBL
Match: B9HPS3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_558151 PE=4 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 1.521e-47
Identity = 102/230 (44.35%), Postives = 140/230 (60.87%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSR---------TQTDINQHTGTHQVSRLLEQG----XXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVA 656
            +DCNICLD+A+DPVLTCCGHLFCWPCFYQLSYVYS  KECPVC  EVT++ IIPIYGNGN+  + +  +KE+GL+VPPRP A R+ES+RQ+LI +G  SSSI + +R F N +   GE  + E   +T  +    +L+ ++         T  +++Q   +  VSRLL QG                      ERLV+DLE+ +H +    +   ++R  +R S  S+ A
Sbjct:  129 FDCNICLDMAQDPVLTCCGHLFCWPCFYQLSYVYSNVKECPVCMEEVTDTSIIPIYGNGNSNDNNKHRLKESGLKVPPRPSAQRVESVRQQLINRGVFSSSIEERMRHFGNVLIAMGEIPRSEDLDDTHLESERISLLENQAITSLALPSTGAEVSQDYHSVHVSRLLFQGAASLSSFSSAVNSAMNSAVESAERLVEDLEAVLHNHRGRRNHHQSSRPADRDSFSSIAA 358

[Close]

BLAST of GT627380 vs. TrEMBL
Match: B9N6I1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_672684 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.868e-46
Identity = 102/237 (43.04%), Postives = 142/237 (59.92%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQT---------DINQHTGTHQVSRLLEQG----XXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVATNRSESR 677
            +DCNICLD+A+DP+LT CGHLFCWPCFYQLSYVYS  KECPVC  EVT++ IIPIYGNGN+  + ++++KE+GL+VPPRP A R+ES+RQ+LI  GA SSSI + +R   N +   GE    E       + +  + + +RT T         D +QH  + QVSRLL QG                      ERL +DL + +H +    + + ++R  +R S  S+ A  + +S+
Sbjct:  130 FDCNICLDMAQDPILTSCGHLFCWPCFYQLSYVYSNVKECPVCVEEVTDTSIIPIYGNGNSYDNKKLKLKESGLKVPPRPSAQRVESVRQQLINHGAFSSSIEERMRYIGNVLIAMGEIPPSEGLDGVPLESDRISFLANRTSTSQALPSIGADSSQHHRSVQVSRLLFQGAASLSSFSSAVNSAMESAMESTERLFEDLGAILHSHRGRRNHQQSSRPADRDSFSSIAAVIQPDSQ 366

[Close]

BLAST of GT627380 vs. TrEMBL
Match: A5APA2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024463 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 4.011e-40
Identity = 91/218 (41.74%), Postives = 127/218 (58.26%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQTDINQHTGTHQVSRLLEQGXXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVA 656
            F+DCNICLDVARDP+LTCCGHLFCWPCFYQL  V+S  KECPVC GEV E+ I PIYG+G+   + ++   + G++ PPRP A RIES+RQ+ + +G  S    +++R   +RI     +   E  +N    +N+ + V   ++ + +Q   + Q SRLL QG                  ERLV+DLE YIH +    +R   + A N   T + +A
Sbjct:  134 FFDCNICLDVARDPILTCCGHLFCWPCFYQLPNVHSNVKECPVCNGEVIETHITPIYGHGS--NNHKVATGDLGVKAPPRPHAHRIESMRQQRVARGIPSFPSEETLRNISSRISRQQARTTTER-SNVLPSQNSTSQVPPGSEAEPSQGLRSVQFSRLLSQGTASFSSLSSALNTALNSAERLVEDLEEYIHSHL---TRSHASSAVNERETLTTIA 345

[Close]

BLAST of GT627380 vs. TrEMBL
Match: D0VEA4_TOBAC (Putative uncharacterized protein (Fragment) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 9.880e-39
Identity = 76/163 (46.63%), Postives = 109/163 (66.87%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQTDINQHTGTHQVSRLLEQG 491
            F+DCNICLD+A++P+LTCCGHL+CWPCFYQL YV S  KECPVCKGEV +  + P+YGNG+  G+   E+ E+GL++PPRPKA R+ES+RQ+ +T+G S   + +++RR    I G G QAQ +       +    + V     T  ++   +  +SR+L +G
Sbjct:   86 FFDCNICLDMAKEPILTCCGHLYCWPCFYQLPYVDSTTKECPVCKGEVADGNVTPVYGNGD--GESITEL-ESGLKIPPRPKARRVESVRQQRVTRGLSHIPVAEALRRIRTSI-GLGHQAQRQDAGGVNLNFVRSSHVLQTADTLSSRRLRSRLISRVLSEG 244

[Close]

BLAST of GT627380 vs. TrEMBL
Match: D7TNU5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_156.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001365001 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.875e-38
Identity = 88/218 (40.37%), Postives = 126/218 (57.80%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQTDINQHTGTHQVSRLLEQGXXXXXXXXXXXXXXXXXXERLVQDLESYIHGNNIVGSRELNTRADNRSSTFSVVA 656
            F+DCNICLD+ARDP+LTCCGHLFCWPCFYQL  V+S  KECP C GEV E+ I PIYG+G+   + ++   + G++ PPRP A RIES+RQ+ + +G  S    +++R   ++I     +   E  +N    +N+ + V   ++ + +Q   + Q SRLL QG                  ERLV+DLE YIH +    +R   + A N   T + +A
Sbjct:   93 FFDCNICLDMARDPILTCCGHLFCWPCFYQLPNVHSNVKECPECNGEVIETHITPIYGHGS--NNHKVATGDLGVKAPPRPHAHRIESMRQQRVARGIPSFPSEETLRIISSQISRQQARTTTER-SNVLPSQNSTSQVPPGSEAEPSQGLRSVQFSRLLSQGTASFSSLSSALNTALNSAERLVEDLEEYIHSHL---TRSHASSAVNERETLTTIA 304

[Close]

BLAST of GT627380 vs. TrEMBL
Match: Q6NLR3_ARATH (At2g44410 OS=Arabidopsis thaliana GN=At2g44410 PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 1.058e-32
Identity = 62/120 (51.67%), Postives = 85/120 (70.83%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQG-----ASSSSIVQSIRRFHNRIG 347
            F+DCNICL+ A DP+LTCCGHLFCW CFYQL  +Y   KECPVC GEVT++ +IPIYGNG+     + ++++ G+ +PPRP A R+ES+RQK+I +G        +  ++ IRR  + IG
Sbjct:  122 FFDCNICLEKAEDPILTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVTDAEVIPIYGNGDDCDGTKPKLEDCGISLPPRPNAKRVESVRQKIINRGNPFFPGHENEAIEHIRRTIDSIG 241

[Close]

BLAST of GT627380 vs. TrEMBL
Match: O64875_ARATH (Putative uncharacterized protein At2g44410 OS=Arabidopsis thaliana GN=At2g44410 PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 1.058e-32
Identity = 62/120 (51.67%), Postives = 85/120 (70.83%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQG-----ASSSSIVQSIRRFHNRIG 347
            F+DCNICL+ A DP+LTCCGHLFCW CFYQL  +Y   KECPVC GEVT++ +IPIYGNG+     + ++++ G+ +PPRP A R+ES+RQK+I +G        +  ++ IRR  + IG
Sbjct:  113 FFDCNICLEKAEDPILTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVTDAEVIPIYGNGDDCDGTKPKLEDCGISLPPRPNAKRVESVRQKIINRGNPFFPGHENEAIEHIRRTIDSIG 232

[Close]

BLAST of GT627380 vs. TrEMBL
Match: D7LLL1_ARALY (Protein binding protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_903861 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 4.021e-32
Identity = 61/121 (50.41%), Postives = 81/121 (66.94%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQA 365
            F+DCNICL+ A DP+LTCCGHLFCW CFYQL  +Y   KECPVC GEVT++ +IPIYGNG+     + +++  G+ +PPRP A R+ES+RQK+I +        ++I      I   G QA
Sbjct:  122 FFDCNICLEKAEDPILTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVTDTEVIPIYGNGDDCDGTKPKLETCGISLPPRPNAKRVESVRQKIINRAIPFPGHDETIEHIRRTIDSIGLQA 242

[Close]

BLAST of GT627380 vs. SwissProt
Match: RMA1_CAPAN (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.072e-18
Identity = 55/165 (33.33%), Postives = 85/165 (51.52%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKE--------CPVCKGEVTESGIIPIYG-NGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQAQLESPTNTRADRNNRTLVRSRTQTDINQHTGTHQVS 473
            +DCNICLD   +PV+T CGHL+CWPC Y+  Y  S + E        CPVCK EV+E  +IP+YG  G +    + +    G+ +P RP +PR       L T  ++ S ++Q  RR      G+ +Q+Q   P    +  ++  L       ++ QH+   +V+
Sbjct:   39 FDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGG-HFLLPTTDSNPSQLLQ--RR------GYQQQSQTRQPAYQGSYMSSPMLSPGGATANMLQHSMIGEVA 194

[Close]

BLAST of GT627380 vs. SwissProt
Match: RMA2_ARATH (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana GN=RMA2 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.321e-18
Identity = 40/96 (41.67%), Postives = 57/96 (59.38%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKE-------------CPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAP 254
            +DCNICLD  RDPV+T CGHLFCWPC ++ +Y  + +++             CPVCK +V+E+ ++PIYG G        +  ++G  VP RP  P
Sbjct:   19 FDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDVSEATLVPIYGRG-------QKAPQSGSNVPSRPTGP 107

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_RAT (RING finger protein 185 OS=Rattus norvegicus GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.641e-17
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   37 FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 116

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_PONAB (RING finger protein 185 OS=Pongo abelii GN=RNF185 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.641e-17
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   37 FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 116

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_MOUSE (RING finger protein 185 OS=Mus musculus GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.641e-17
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   37 FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 116

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_HUMAN (RING finger protein 185 OS=Homo sapiens GN=RNF185 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.641e-17
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   37 FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 116

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_CHICK (RING finger protein 185 OS=Gallus gallus GN=RNF185 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.641e-17
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   39 FECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 118

[Close]

BLAST of GT627380 vs. SwissProt
Match: RN185_DANRE (RING finger protein 185 OS=Danio rerio GN=rnf185 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.883e-17
Identity = 37/86 (43.02%), Postives = 54/86 (62.79%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD ++D V++ CGHLFCWPC +Q        + CPVCK  ++   +IP+YG G+ G   Q + +E   + PPRP+  R E
Sbjct:   39 FECNICLDTSKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG---QQDPRE---KTPPRPQGQRPE 118

[Close]

BLAST of GT627380 vs. SwissProt
Match: RMA3_ARATH (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana GN=RMA3 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.883e-17
Identity = 44/124 (35.48%), Postives = 63/124 (50.81%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVY-------SRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRR------FHN 338
            +DCNICLD A DPV+T CGHLFCWPC Y+  +V             CPVCK  +T + ++P+YG G +        K+        P+ P   ++R  +     S+SS+  S++       FHN
Sbjct:   42 FDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPI----TSASSLNPSLQHQTLSPSFHN 161

[Close]

BLAST of GT627380 vs. SwissProt
Match: RNF5_CAEEL (RING finger protein 5 OS=Caenorhabditis elegans GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.712e-16
Identity = 38/86 (44.19%), Postives = 50/86 (58.14%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIE 263
            ++CNICLD A+D V++ CGHLFCWPC  Q        + CPVCK  +  + ++PIYG G    D +        +VPPRPK  R E
Sbjct:   24 FECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCPVCKSAIDGNKVVPIYGRGGDSSDPRK-------KVPPRPKGQRSE 102

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT2G44410.1 (| Symbols: | RING/U-box superfamily protein | chr2:18328873-18330114 FORWARD LENGTH=413)

HSP 1 Score: 144.436 bits (363), Expect = 6.795e-35
Identity = 62/120 (51.67%), Postives = 85/120 (70.83%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQG-----ASSSSIVQSIRRFHNRIG 347
            F+DCNICL+ A DP+LTCCGHLFCW CFYQL  +Y   KECPVC GEVT++ +IPIYGNG+     + ++++ G+ +PPRP A R+ES+RQK+I +G        +  ++ IRR  + IG
Sbjct:  122 FFDCNICLEKAEDPILTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVTDAEVIPIYGNGDDCDGTKPKLEDCGISLPPRPNAKRVESVRQKIINRGNPFFPGHENEAIEHIRRTIDSIG 241

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT3G58030.4 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 120.168 bits (300), Expect = 1.372e-27
Identity = 60/114 (52.63%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEA-GLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNR 341
            F+DCNICLD++++PVLTCCGHL+CWPC YQ   + S AKECPVCKGEVT   + PIYG GN     + E++E+   +VP RP A RIES+R   I +   +  + + IRR  NR
Sbjct:  136 FFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKECPVCKGEVTSKTVTPIYGRGNH----KREIEESLDTKVPMRPHARRIESLR-NTIQRSPFTIPMEEMIRRIQNR 243

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT3G58030.3 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 120.168 bits (300), Expect = 1.372e-27
Identity = 60/114 (52.63%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEA-GLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNR 341
            F+DCNICLD++++PVLTCCGHL+CWPC YQ   + S AKECPVCKGEVT   + PIYG GN     + E++E+   +VP RP A RIES+R   I +   +  + + IRR  NR
Sbjct:  136 FFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKECPVCKGEVTSKTVTPIYGRGNH----KREIEESLDTKVPMRPHARRIESLR-NTIQRSPFTIPMEEMIRRIQNR 243

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT3G58030.2 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 120.168 bits (300), Expect = 1.372e-27
Identity = 60/114 (52.63%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEA-GLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNR 341
            F+DCNICLD++++PVLTCCGHL+CWPC YQ   + S AKECPVCKGEVT   + PIYG GN     + E++E+   +VP RP A RIES+R   I +   +  + + IRR  NR
Sbjct:  136 FFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKECPVCKGEVTSKTVTPIYGRGNH----KREIEESLDTKVPMRPHARRIESLR-NTIQRSPFTIPMEEMIRRIQNR 243

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT3G58030.1 (| Symbols: | RING/U-box superfamily protein | chr3:21485527-21486837 FORWARD LENGTH=436)

HSP 1 Score: 120.168 bits (300), Expect = 1.372e-27
Identity = 60/114 (52.63%), Postives = 78/114 (68.42%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEA-GLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNR 341
            F+DCNICLD++++PVLTCCGHL+CWPC YQ   + S AKECPVCKGEVT   + PIYG GN     + E++E+   +VP RP A RIES+R   I +   +  + + IRR  NR
Sbjct:  136 FFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKECPVCKGEVTSKTVTPIYGRGNH----KREIEESLDTKVPMRPHARRIESLR-NTIQRSPFTIPMEEMIRRIQNR 243

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT2G42030.1 (| Symbols: | RING/U-box superfamily protein | chr2:17539069-17540346 REVERSE LENGTH=425)

HSP 1 Score: 103.99 bits (258), Expect = 1.018e-22
Identity = 52/120 (43.33%), Postives = 73/120 (60.83%), Query Frame = 3

Query:    3 FYDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSSSIVQSIRRFHNRIGGFGEQ 362
            F+DC ICLD+++DPV+T CGHL+CW C YQ   V S AKECPVCKGEV+   + PIYG G    + +   + +  ++P RP+A R ES+R  L   G   + I + ++    R    GE+
Sbjct:  138 FFDCYICLDLSKDPVVTNCGHLYCWSCLYQWLQV-SEAKECPVCKGEVSVKTVTPIYGRGIQKRESE---EVSNTKIPSRPQARRTESLRTTLNRSGHIPTEIFRHLQDRLERESSTGER 253

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT1G19310.1 (| Symbols: | RING/U-box superfamily protein | chr1:6676424-6677104 REVERSE LENGTH=226)

HSP 1 Score: 103.99 bits (258), Expect = 1.018e-22
Identity = 41/87 (47.13%), Postives = 62/87 (71.26%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIES 266
            ++CNICLD+A+DP++T CGHLFCWPC Y+  +++S++K+CPVCK  + E  ++P+YG G +  D + +    GL VP RP   R E+
Sbjct:   21 FECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCKAVIEEDRLVPLYGRGKSSADPRSK-SIPGLEVPNRPSGQRPET 106

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT1G74990.1 (| Symbols: | RING/U-box superfamily protein | chr1:28159837-28160250 REVERSE LENGTH=137)

HSP 1 Score: 103.605 bits (257), Expect = 1.329e-22
Identity = 44/100 (44.00%), Postives = 66/100 (66.00%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIESIRQKLITQGASSS 305
            + CNICL++AR+P++T CGHLFCWPC Y+  + +S++  CPVCK  V E  ++P+YG G    D + ++  +G+ VP RP A R E+ R +L  +   SS
Sbjct:   17 FGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALVKEDTLVPLYGMGKPSSDPRSKL-NSGVTVPNRPAATRTETARPRLEQRHHGSS 115

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT2G23780.1 (| Symbols: | RING/U-box superfamily protein | chr2:10123551-10124234 REVERSE LENGTH=227)

HSP 1 Score: 97.4413 bits (241), Expect = 9.526e-21
Identity = 39/87 (44.83%), Postives = 60/87 (68.97%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKECPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAPRIES 266
            ++CNIC ++A+DP++T CGHLFCWPC Y+  + +S ++ECPVCK  V +  ++P+YG G    D + + +  GLR+P RP   R E+
Sbjct:   26 FECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQECPVCKAVVQDDKLVPLYGRGKNQTDPRSK-RYPGLRIPNRPTGQRPET 111

[Close]

BLAST of GT627380 vs. TAIR peptide
Match: AT4G28270.1 (| Symbols: ATRMA2, RMA2 | RING membrane-anchor 2 | chr4:14007614-14008195 REVERSE LENGTH=193)

HSP 1 Score: 91.6633 bits (226), Expect = 5.227e-19
Identity = 40/96 (41.67%), Postives = 57/96 (59.38%), Query Frame = 3

Query:    6 YDCNICLDVARDPVLTCCGHLFCWPCFYQLSYVYSRAKE-------------CPVCKGEVTESGIIPIYGNGNAGGDCQMEMKEAGLRVPPRPKAP 254
            +DCNICLD  RDPV+T CGHLFCWPC ++ +Y  + +++             CPVCK +V+E+ ++PIYG G        +  ++G  VP RP  P
Sbjct:   19 FDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDVSEATLVPIYGRG-------QKAPQSGSNVPSRPTGP 107

The following BLAST results are available for this feature:

BLAST of GT627380 vs. TrEMBL
BLAST of GT627380 vs. SwissProt
BLAST of GT627380 vs. TAIR peptide

BLAST of GT627380 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TEZ0_SOYBN	5.203e-64	50.55	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TIN8_SOYBN	4.404e-63	51.10	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9HPS3_POPTR	1.521e-47	44.35	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N6I1_POPTR	2.868e-46	43.04	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5APA2_VITVI	4.011e-40	41.74	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D0VEA4_TOBAC	9.880e-39	46.63	Putative uncharacterized protein (Fragment) OS=Nic...	[more]
D7TNU5_VITVI	2.875e-38	40.37	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q6NLR3_ARATH	1.058e-32	51.67	At2g44410 OS=Arabidopsis thaliana GN=At2g44410 PE=...	[more]
O64875_ARATH	1.058e-32	51.67	Putative uncharacterized protein At2g44410 OS=Arab...	[more]
D7LLL1_ARALY	4.021e-32	50.41	Protein binding protein OS=Arabidopsis lyrata subs...	[more]

BLAST of GT627380 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RMA1_CAPAN	1.072e-18	33.33	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum ann...	[more]
RMA2_ARATH	5.321e-18	41.67	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis th...	[more]
RN185_RAT	2.641e-17	44.19	RING finger protein 185 OS=Rattus norvegicus GN=Rn...	[more]
RN185_PONAB	2.641e-17	44.19	RING finger protein 185 OS=Pongo abelii GN=RNF185 ...	[more]
RN185_MOUSE	2.641e-17	44.19	RING finger protein 185 OS=Mus musculus GN=Rnf185 ...	[more]
RN185_HUMAN	2.641e-17	44.19	RING finger protein 185 OS=Homo sapiens GN=RNF185 ...	[more]
RN185_CHICK	2.641e-17	44.19	RING finger protein 185 OS=Gallus gallus GN=RNF185...	[more]
RN185_DANRE	5.883e-17	43.02	RING finger protein 185 OS=Danio rerio GN=rnf185 P...	[more]
RMA3_ARATH	5.883e-17	35.48	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis th...	[more]
RNF5_CAEEL	1.712e-16	44.19	RING finger protein 5 OS=Caenorhabditis elegans GN...	[more]

BLAST of GT627380 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G44410.1	6.795e-35	51.67	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.4	1.372e-27	52.63	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.3	1.372e-27	52.63	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.2	1.372e-27	52.63	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT3G58030.1	1.372e-27	52.63	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT2G42030.1	1.018e-22	43.33	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT1G19310.1	1.018e-22	47.13	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT1G74990.1	1.329e-22	44.00	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT2G23780.1	9.526e-21	44.83	\| Symbols: \| RING/U-box superfamily protein \| chr...	[more]
AT4G28270.1	5.227e-19	41.67	\| Symbols: ATRMA2, RMA2 \| RING membrane-anchor 2 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	no description	coord: 4..44 score: 3
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 4..45 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	no description	coord: 3..55 score: 1.5
None	No IPR available	PANTHER	PTHR12313	RNF5	coord: 1..207 score: 2.1
None	No IPR available	SEG	seg	seg	coord: 164..181 score: NAcoord: 226..248 score
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 4..65 score: 8.6
None	No IPR available	SEG	seg	seg	coord: 40..58 score
None	No IPR available	SEG	seg	seg	coord: 31..47 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627380 ID=GT627380; Name=GT627380; organism=Lens culinaris; type=EST; length=804bp
GTTTTTATGACTGTAATATATGTTTGGATGTCGCGAGAGATCCTGTTTTG
ACGTGTTGTGGGCATTTGTTTTGTTGGCCGTGTTTCTATCAGTTATCGTA
TGTTTATTCGAGAGCGAAGGAGTGTCCTGTTTGTAAAGGAGAGGTTACCG
AATCGGGTATTATTCCGATTTATGGGAATGGTAATGCCGGTGGTGATTGT
CAGATGGAAATGAAAGAGGCCGGTTTGCGAGTTCCTCCTCGACCTAAGGC
TCCTAGAATTGAGAGTATTAGACAGAAGTTGATAACCCAAGGTGCATCTT
CTTCTTCTATTGTCCAAAGTATTCGGCGGTTTCATAACCGGATTGGTGGA
TTTGGAGAACAAGCTCAGTTAGAAAGCCCTACTAATACCAGAGCTGATAG
AAATAATCGTACGCTCGTCCGTTCTCGCACGCAGACTGATATTAATCAAC
ATACTGGCACTCATCAGGTTTCAAGGTTGCTAGAACAAGGAGCTTCTTCA
TTTTCATCGCTGTCATCAGCATTAAATTCGGCAATGGATTCTGCAGAAAG
ACTAGTTCAGGACCTTGAATCTTATATCCACGGCAACAATATAGTAGGAA
GTAGAGAGCTAAACACTCGTGCTGATAATAGAAGTTCAACGTTTAGTGTT
GTTGCTACGAACCGCTCAGAGAGTCGTGCTCAAGATGTTGTTGATGTGGC
CAATTCTGCTGCAGCAGCAGCTAGGAATGTTGACAATATTGCTGTTAGTG
GCTCAGAAATTCAGACAACTGATAGAAACATACAGATTAGTCCGTTAGAC
CCTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

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