GT627884

EST Overview


Name	GT627884
Unique Name	GT627884
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	480

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B7FIR2_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.574e-22
Identity = 50/67 (74.63%), Postives = 58/67 (86.57%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IEEFSC  K  SNALKEV+ V+K+LYD D LEEEHILQWYQEGLKGD K++QIWKNVKP ++WLQ+A
Sbjct:  157 IEEFSC--KSTSNALKEVALVLKALYDADALEEEHILQWYQEGLKGDTKNAQIWKNVKPFIEWLQSA 221

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B9H349_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_648073 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.538e-20
Identity = 43/59 (72.88%), Postives = 53/59 (89.83%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  S+A+KEV+ ++K+LYD DVLEEE+I+QWYQEGLKG NKDSQIWKN KPV++WLQNA
Sbjct:  387 KSGSSAMKEVALILKTLYDADVLEEEYIVQWYQEGLKGSNKDSQIWKNAKPVIEWLQNA 445

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B9N7N9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_584639 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.234e-18
Identity = 41/59 (69.49%), Postives = 51/59 (86.44%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  S+ALKEV+ V+K LYD DVLEEE+++QWYQEGL+G +KDSQIWKN KP ++WLQNA
Sbjct:  379 KSSSSALKEVALVMKDLYDADVLEEEYMVQWYQEGLEGPSKDSQIWKNAKPFIEWLQNA 437

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B9NCP7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_590689 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.431e-15
Identity = 39/59 (66.10%), Postives = 47/59 (79.66%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  S+AL+EV  V+K+LYD DVLEEE I+ WYQEGL   NKDSQIWKNV+P + WLQ+A
Sbjct:  390 KSSSSALREVGLVLKALYDADVLEEECIVHWYQEGLARGNKDSQIWKNVEPFIKWLQSA 448

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B9GV94_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_553039 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.431e-15
Identity = 39/59 (66.10%), Postives = 47/59 (79.66%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  S+AL+EV  V+K+LYD DVLEEE I+ WYQEGL   NKDSQIWKNV+P + WLQ+A
Sbjct:  390 KSSSSALREVGLVLKALYDADVLEEECIVHWYQEGLARGNKDSQIWKNVEPFIKWLQSA 448

[Close]

BLAST of GT627884 vs. TrEMBL
Match: A5AT95_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030619 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.416e-15
Identity = 39/67 (58.21%), Postives = 51/67 (76.12%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IE F    +P  +ALKEV+ V+K LYD DVLEEE+I+QWY+EG+ G NK SQIWKN +P + W+Q+A
Sbjct:  388 IEAFCGKSRP--SALKEVALVLKGLYDGDVLEEEYIVQWYEEGVSGANKKSQIWKNAQPFITWIQSA 452

[Close]

BLAST of GT627884 vs. TrEMBL
Match: B9RJC4_RICCO (Eukaryotic translation initiation factor, putative OS=Ricinus communis GN=RCOM_1033150 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.688e-14
Identity = 40/67 (59.70%), Postives = 47/67 (70.15%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IE F     P   A KEVS V+K LYD DVLEE+ IL WYQ+GL G NK+S +WK VKP V+WL+NA
Sbjct:  376 IESFFAKLSP--EATKEVSLVLKVLYDNDVLEEDFILAWYQKGLSGSNKNSAVWKYVKPFVEWLENA 440

[Close]

BLAST of GT627884 vs. TrEMBL
Match: D7KV46_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_476949 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.972e-13
Identity = 33/62 (53.23%), Postives = 45/62 (72.58%), Query Frame = 3

Query:   18 CSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            C       ALKEV+ V+K+LYD D++EEE +L WY++G  G +K S +WKNVKP V+WLQ+A
Sbjct:  371 CGKNGNEEALKEVALVLKALYDQDIIEEEFVLDWYEKGFTGVDKSSPVWKNVKPFVEWLQSA 432

[Close]

BLAST of GT627884 vs. TrEMBL
Match: A3RCW1_WHEAT (Translation initiation factor eIF5 OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.631e-11
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IE F   C   + ALKE+  V+K+LYD DVLEEE I+QWY E +    KDSQI KN KPVV+WLQ+A
Sbjct:  386 IEAFCGKCS--AEALKELPVVLKALYDGDVLEEETIVQWYNEAVAA-GKDSQIVKNAKPVVEWLQSA 449

[Close]

BLAST of GT627884 vs. TrEMBL
Match: C0P4L3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.057e-10
Identity = 35/67 (52.24%), Postives = 44/67 (65.67%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IE F   C P   ALKEV  V+K+LYD D+LEEE ++ WY   +    KDSQ+ KN KP V+WLQ+A
Sbjct:  382 IEAFGSKCNP--EALKEVPVVLKALYDGDILEEETVVDWYNAAVAA-GKDSQVVKNAKPFVEWLQSA 445

[Close]

BLAST of GT627884 vs. SwissProt
Match: IF5_PHAVU (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris GN=EIF5 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 7.973e-22
Identity = 48/67 (71.64%), Postives = 57/67 (85.07%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IEEF C  KP SNALKEV+ V+K+LYD DVLEEE I+ WYQ+GLKGDNK+S+IWKN +P +DWLQNA
Sbjct:  372 IEEFCC--KPNSNALKEVALVLKTLYDADVLEEEAIVLWYQKGLKGDNKNSKIWKNAQPFIDWLQNA 436

[Close]

BLAST of GT627884 vs. SwissProt
Match: IF5Z_ARATH (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana GN=At1g77840 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 9.440e-15
Identity = 34/62 (54.84%), Postives = 46/62 (74.19%), Query Frame = 3

Query:   18 CSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            C       ALKEV+ V+K+LYD D++EEE +L WY++GL G +K S +WKNVKP V+WLQ+A
Sbjct:  368 CGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLTGADKSSPVWKNVKPFVEWLQSA 429

[Close]

BLAST of GT627884 vs. SwissProt
Match: IF5_MAIZE (Eukaryotic translation initiation factor 5 OS=Zea mays GN=EIF5 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 9.769e-12
Identity = 35/67 (52.24%), Postives = 45/67 (67.16%), Query Frame = 3

Query:    3 IEEFSCSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            IE F   C P   ALKEV  V+K+LYD D+L+EE I+ WY + +    KDSQ+ KN KP V+WLQ+A
Sbjct:  379 IEAFGGKCNP--EALKEVPVVLKALYDGDILDEETIVDWYNDAVAA-GKDSQVVKNAKPFVEWLQSA 442

[Close]

BLAST of GT627884 vs. SwissProt
Match: IF5Y_ARATH (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.560e-9
Identity = 28/59 (47.46%), Postives = 41/59 (69.49%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  + A KEV+ V+K LYD D+L+E+ I++WY +G+    K S + KNV P ++WLQNA
Sbjct:  378 KASAEAAKEVALVIKGLYDEDILDEDVIVEWYNKGV----KSSPVLKNVTPFIEWLQNA 432

[Close]

BLAST of GT627884 vs. TAIR peptide
Match: AT1G77840.1 (| Symbols: | Translation initiation factor IF2/IF5 | chr1:29269087-29270400 FORWARD LENGTH=437)

HSP 1 Score: 79.337 bits (194), Expect = 1.095e-15
Identity = 34/62 (54.84%), Postives = 46/62 (74.19%), Query Frame = 3

Query:   18 CSCKPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            C       ALKEV+ V+K+LYD D++EEE +L WY++GL G +K S +WKNVKP V+WLQ+A
Sbjct:  368 CGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLTGADKSSPVWKNVKPFVEWLQSA 429

[Close]

BLAST of GT627884 vs. TAIR peptide
Match: AT1G36730.1 (| Symbols: | Translation initiation factor IF2/IF5 | chr1:13898706-13900025 REVERSE LENGTH=439)

HSP 1 Score: 62.003 bits (149), Expect = 1.809e-10
Identity = 28/59 (47.46%), Postives = 41/59 (69.49%), Query Frame = 3

Query:   27 KPPSNALKEVSYVVKSLYDVDVLEEEHILQWYQEGLKGDNKDSQIWKNVKPVVDWLQNA 203
            K  + A KEV+ V+K LYD D+L+E+ I++WY +G+    K S + KNV P ++WLQNA
Sbjct:  378 KASAEAAKEVALVIKGLYDEDILDEDVIVEWYNKGV----KSSPVLKNVTPFIEWLQNA 432

The following BLAST results are available for this feature:

BLAST of GT627884 vs. TrEMBL
BLAST of GT627884 vs. SwissProt
BLAST of GT627884 vs. TAIR peptide

BLAST of GT627884 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FIR2_MEDTR	4.574e-22	74.63	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9H349_POPTR	9.538e-20	72.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N7N9_POPTR	5.234e-18	69.49	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9NCP7_POPTR	2.431e-15	66.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GV94_POPTR	2.431e-15	66.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AT95_VITVI	5.416e-15	58.21	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9RJC4_RICCO	2.688e-14	59.70	Eukaryotic translation initiation factor, putative...	[more]
D7KV46_ARALY	2.972e-13	53.23	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A3RCW1_WHEAT	1.631e-11	58.21	Translation initiation factor eIF5 OS=Triticum aes...	[more]
C0P4L3_MAIZE	1.057e-10	52.24	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GT627884 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
IF5_PHAVU	7.973e-22	71.64	Eukaryotic translation initiation factor 5 OS=Phas...	[more]
IF5Z_ARATH	9.440e-15	54.84	Probable eukaryotic translation initiation factor ...	[more]
IF5_MAIZE	9.769e-12	52.24	Eukaryotic translation initiation factor 5 OS=Zea ...	[more]
IF5Y_ARATH	1.560e-9	47.46	Probable eukaryotic translation initiation factor ...	[more]

BLAST of GT627884 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G77840.1	1.095e-15	54.84	\| Symbols: \| Translation initiation factor IF2/IF...	[more]
AT1G36730.1	1.809e-10	47.46	\| Symbols: \| Translation initiation factor IF2/IF...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003307	eIF4-gamma/eIF5/eIF2-epsilon	PFAM	PF02020	W2	coord: 6..74 score: 1.8
IPR003307	eIF4-gamma/eIF5/eIF2-epsilon	SMART	SM00515	no description	coord: 1..70 score: 1.2
IPR003307	eIF4-gamma/eIF5/eIF2-epsilon	PROFILE	PS51363	W2	coord: 1..74 score: 18
IPR016021	MIF4-like, type 1/2/3	GENE3D	1.25.40.180	no description	coord: 10..74 score: 3.3
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 1..68 score: 3.5
None	No IPR available	PANTHER	PTHR23001	EUKARYOTIC TRANSLATION INITIATION FACTOR	coord: 12..74 score: 7.6
None	No IPR available	PANTHER	PTHR23001:SF1	EUKARYOTIC TRANSLATION INITIATION FACTOR 5	coord: 12..74 score: 7.6

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT627884 ID=GT627884; Name=GT627884; organism=Lens culinaris; type=EST; length=480bp
CAATTGAGGAATTTTCTTGTTCTTGCAAGCCCCCTTCCAACGCACTTAAG
GAGGTTTCCTATGTTGTAAAGTCGCTTTATGATGTTGATGTTTTGGAGGA
AGAACACATATTGCAGTGGTATCAGGAAGGGCTGAAAGGAGACAATAAGG
ACTCTCAGATTTGGAAGAATGTTAAGCCCGTCGTTGACTGGCTTCAGAAT
GCAGAATCAGAGTCGGAGGAAGAATAGTGCGTTGGAGGGTTTGTTTATGT
CTGCGACTTTCTCGTGTCTTTTTGTTAATAAAGGGTGTGGATTTCCCCTA
GTGTTGAGCGTTTGTTTTAAAACTTTAAATTGTGATGTTTGCCTTTTGTT
TATGTCTCTTAAAGTTATGTGATCTGAAACACTCTTCTGTGTTTGAGCTT
TGTCATAGAACATGGAATTCTGGTGTGTTTGTGTTTGTTTAACCGTTTCT
GGTATTATTATAATGTTATTTTATCTGGTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016070	RNA metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR003307	W2_domain
IPR016021	MIF4-like_sf
IPR016024	ARM-type_fold

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